Wyniki wyszukiwania

1

Liderzy wiejscy w procesie aktywizacji społeczności lokalnych

100%

Kalemba M.

Zagadnienia Doradztwa Rolniczego

|

1997

|

nr 2

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The estrogenic status and the COMT genotype of female blood donors influence the protective ability of the Mediterranean plant extracts against the hydrogen peroxide-induced DNA damage in lymphocytes

51%

Kapiszewska M., Zajac G., Kalemba M., Soltys E.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 1

199-217

The extent of oxidative DNA damage in lymphocytes can be used as a biomarker of the level of oxidative stress in the body. The comet assay has been widely used to measure such damage. The aim of our study was to evaluate: i) the extent of the oxidative DNA damage in lymphocytes isolated from blood of female donors taken in early and late follicular phases [low (LE) and high (HE) concentration of 17bestradiol, respectively], ii) the susceptibility of these lymphocytes to hydrogen peroxide exposure, and iii) the protective ability of five plant extracts against the hydrogen peroxide-induced DNA damage. The effect of the catechol-Omethytransferase genotype (wild COMT H/H and mutated homozygote COMT L/L) of female donors was also analyzed. The amount of endogenous DNA damage was higher in HE lymphocytes as compared with LE ones, independently of the genotype. When lymphocytes were stratified by COMT genotype, the level of DNA damage was higher in L/L donors. The protective effect of pretreatment with plant extracts (1 and 10 µg/ml for 1 h) against the H2O2 (25 µM, 5 min. at 4°C)-induced oxidative DNA damage was observed only in H/H HE lymphocytes. In contrary, the plant extract pre-incubation enhanced the DNA damage in L/L HE lymphocytes. The plant extracts alone did not induce the DNA damage. The results showed that concentration of the circulating 17b-estradiol influenced the extent of endogenous oxidative DNA damage while the beneficial or hazardous effects of the plant extracts might depend on the COMT genotype and the estrogen level.

3

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women

51%

Kapiszewska M., Kalemba M., Grzesiak A., Kocemba K.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

The aim of this study was to evaluate whether the differences in plasma 17β-estradiol concentration in early and late follicular phases of the menstrual cycle can affect the level of endogenous DNA damage in lymphocytes assessed by comet assay, and whether the extent of this damage in the follicular phase is associated with the genotype of catechol-O-methyltransferase (COMT). The level of DNA damage was positively correlated with 17β-estradiol concentration only in the late follicular phase. Subjects with the COMT L/L homozygous mutated variant revealed more DNA damage as compared to individuals with the COMT wild-type and heterozygous (H/L+HH) genotype.

4

Wieś w nowej rzeczywistości

26%

Kalemba M.

Przegląd Wielkopolski

|

1996

|

tom 10

|

nr 3-4

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology. Supplement

1 Przegląd Wielkopolski

1 Zagadnienia Doradztwa Rolniczego

4 Kalemba M.

2 Kapiszewska M.

1 Grzesiak A.

1 Kocemba K.

1 Soltys E.

1 Zajac G.

2 2005

1 1997

1 1996

Liderzy wiejscy w procesie aktywizacji społeczności lokalnych

The estrogenic status and the COMT genotype of female blood donors influence the protective ability of the Mediterranean plant extracts against the hydrogen peroxide-induced DNA damage in lymphocytes

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women

Wieś w nowej rzeczywistości