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Radiotoxic effects of cancer cells irradiated in vitro on neighbour nonirradiated cells

100%

Poloniak R., Szurko A., Widel M., Maniakowski Z., Birkner E., Przybyszewski W.

Acta Biochimica Polonica

2003

tom 50

nr Supplement 1

Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro

100%

Szurko A., Kramer-Marek G., Widel M., Ratuszna A., Habdas J., Kus P.

Acta Biochimica Polonica

2003

tom 50

nr 4

Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 ug/ml (42.14 uM) and P2 at concentrations up to 20 ug/ml (16.86 uM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Szurko A.

2 Widel M.

1 Birkner E.

1 Habdas J.

1 Kramer-Marek G.

1 Kus P.

1 Maniakowski Z.

1 Poloniak R.

1 Przybyszewski W.

1 Ratuszna A.

2 2003

Wyniki wyszukiwania

Radiotoxic effects of cancer cells irradiated in vitro on neighbour nonirradiated cells

Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro