Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 33

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Badania nad wystepowaniem mykoplazmowego zapalenia pluc swin na terenie Polski

100%
Wasinski B.
Trzoda Chlewna
|
1995
|
tom 33
|
nr 05
52-53
2

Możliwości rozpoznawania zakażeń Leptospira spp. u świń z użyciem reakcji łańcuchowej polimerazy (PCR)

100%
Wasinski B.
Medycyna Weterynaryjna
|
2015
|
tom 71
|
nr 09
Leptospirosis can be an important problem and a cause of significant economic losses in swine herds. Because of a high susceptibility of leptospires to many factors, laboratory diagnosis of the disease (especially pathogen isolation and identification) is difficult and often based on serological methods. The aim of this study was to show the possible use of PCR for detection and partial identification of Leptospira spp. in clinical samples from swine. Four aborted fetuses and 2 serum samples from sows reared on a farm infected by the serovar Pomona and 4 fetuses from a farm infected by leptospires from the serogroup Sejroe were submitted for examination. Additionally, urine and serum samples from 8 aborting sows reared on 2 farms infected by the serogroup Sejroe were investigated. Serum samples were examined by the microagglutination test (MAT). Samples of urine and tissue samples from fetuses were examined by PCR with pairs of primers detecting DNA sequences specific to a) genus Leptospira, b) species L. borgpetersenii, c) two selected groups of serovars of the species L. interrogans, d) serogroup Sejroe. Serological findings showed in all examined sows the presence of titers to the serogroup Pomona or Sejroe, depending on the serogroup causing infection on a given farm. DNA of the genus Leptospira was detected in tissues of all fetuses from sows infected by the serovar Pomona, in 3 fetuses from the farm infected by the Sejroe serogroup, and in all urine samples. The presence of the DNA sequence specific for the group of L. interrogans serovars including the serovar Pomona was found in tissues of all 4 fetuses from dams presenting titers to the serogroup Pomona. DNA of the serogroup Sejroe was detected in 6 out of 8 urine samples examined, and DNA of the species L. borgpetersenii (including the serovar Sejroe) was found in 5 urine samples. No DNA of the species L. borgpetersenii or of the serogroup Sejroe was found in tissues of fetuses from the farm infected by the serogroup Sejroe. This study demonstrated the usefulness of PCR in confirming the presence of Leptospira spp. in clinical samples from swine. Furthermore, PCR confirmed the presence of the serovar Pomona in tissues of aborted fetuses and the presence of L. borgpetersenii and/or the Sejroe serogroup in samples of urine. A conclusive evaluation of the usefulness of PCR in identifying DNA of L. borgpetersenii and the serogroup Sejroe in tissue samples requires further investigations.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Extra-intestinal pathogenic Escherichia coli – threat connected with food-borne infections

100%
Wasinski B.
Annals of Agricultural and Environmental Medicine
|
2019
|
tom 26
|
nr 4
4

Nowe metody laboratoryjnej diagnostyki leptospirozy

100%
Wasinski B.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 03
266-270
5

Contribution of Clostridium perfringens type A with beta2 toxin gene in aetiology of porcine enteric diseases. A case report

100%
Wasinski B.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 4
6

Wlasciwosci i chorobotworczosc toksyny beta-2 Clostridium perfringens

100%
Wasinski B.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 11
1275-1279
Described recently and investigated intensively over the last years, β2 toxin (CPB2) is produced by all toxino-types of Clostridium perfringens. The ability of CPB2 production was found in C. perfringens strains isolated from humans and many species of domestic animals and wildlife. The cpb2 gene encoding CPB2 was found in strains isolated from pathological cases and from animals without clinical symptoms. Apart from the detection of cpb2 presence, the demonstration of the expression of the gene is critical for laboratory diagnosis. CPB2 is postulated to participate in the development of differing in intensity enteric inflammatory changes. Its contribution to other C. perfringens toxins through the facilitating of their absorption is also suggested. Current data concerning regulation of cpb2 expression, CPB2 structure, its properties and presumptive role in pathogenesis of C. perfringens infections were reviewed in the present paper. Additionally some case reports concerning the putative role of CPB2 were reviewed.
7

Wystepowanie zakazen bakteriami z rodzaju Leptospira u swin w latach 2002-2003

100%
Wasinski B.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 01
46-49
8

Infections of swine caused by Leptospira serovars of serogroup Sejroe - possibilities of recognition with the use of PCR

100%
Wasinski B.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 4
The aim of this study was to confirm the presence of leptospires from the Sejroe serogroup in aborting seropositive sows and to identify which serovars of this serogroup are responsible for the infection. Serum and urine samples from 20 aborting sows, reared in five farms infected with the Sejroe serogroup, were submitted for examination. Serum samples were examined by the microscopic agglutination test (MAT) and urine samples by polymerase chain reaction (PCR) with primer sets specific for genus Leptospira, species L. borgpetersenii, the Sejroe serogroup, and the Hardjo-bovis genotype. All the examined serum samples showed titres specific to the Sejroe serogroup, ranging from 100 to 6400. PCR indicated the presence of Leptospira genus-specific DNA sequences in all urine samples examined. DNA sequences specific for both L. borgpetersenii and the Sejroe serogroup were found in 12 of these samples. Additionally, the sequences specific only for L. borgpetersenii were found in five urine samples and sequences for the Sejroe serogroup in two samples. The DNA sequence specific for the Hardjo-bovis genotype was not found in the urine samples. PCR confirmed the results of the MAT, displayed the presence of Leptospira sp. DNA in the urine of all the sows examined, proved the affinity of the detected leptospires to the Sejroe serogroup or at least to L. borgpetersenii, and excluded the Hardjo serovar as the reason for the infections described in the sows.
9

Uwazajmy na leptospiroze

100%
Wasinski B.
Trzoda Chlewna
|
1999
|
tom 37
|
nr 08-09
126
10

Occurrence of Leptospira sp. antibodies in swine in Poland

100%
Wasinski B.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
The aim of the study was to evaluate the prevalence of antibodies specific to different serogroups of Leptospira sp. in swine populations in Poland. The serological survey was conducted in 2006. A total of 7 252 swine serum samples coming from all provinces of the country were investigated. The samples were examined by the use of a microscopic agglutination test. As antigens, six Leptospira serovars were used: Leptospira interrogans serovars - Icterohaemorrhagiae, Pomona, and Canicola, L. borgpetersenii serovars - Sejroe and Tarassovi, and L. kirschneri serovar Grippotyphosa. Altogether, 97 (1.34%) of the examined serum samples were found to be positive. They were obtained from 12 provinces. The highest number of samples among positive sera demonstrated titres to serovar Sejroe (44.33%) followed by Pomona (37.11%), Icterohaemorrhagiae (8.25%), Canicola (7.22%), and Tarassovi (3.09%). No samples with titres to serovar Grippotyphosa were found. The data concerning the highness titres to particular serovars and the prevalence of animals demonstrating antibodies to different serovars in particular provinces are presented and analysed in the article.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Leptospirosis - current risk factors connected with human activity and the environment

63%
Wasinski B., Dutkiewicz J.
Annals of Agricultural and Environmental Medicine
|
2013
|
tom 20
|
nr 2
Leptospirosis is a widespread although recently neglected zoonosis recognized worldwide. The disease seems to be underestimated, especially in countries located in the temperate climatic zone. The presented article concerns the main characteristics of leptospirosis and describes formerly known and recently observed environmental, occupational and recreational risk factors significant in the spreading and pathogenesis of the disease. The aspects of epidemiology significant in the temperate climatic zone are emphasized. The majority of cited articles present cases of the disease reported from Europe or North America. Climatic changes (warming) and extreme weather events such as floods are potential risk factors of leptospirosis. Also, some socio-economic phenomena, such as the intensive migration of people resulting in the transfer of the infections acquired in tropical countries, or worsening of economic status in the cities, increase the probability of disease. Apart from the danger connected with rodents, which are the main vectors of leptospires, occurrence of the disease in dogs and cats can generate a higher risk of infection for humans. Infections may also be acquired during various types of agricultural work and during recreational activities, such as swimming. The results of recent investigations show that ticks are also potential vectors of leptospires. The more frequent emergence of leptospirosis in countries located in the temperate climatic zone emphasize the need to verify knowledge related to the risk of its appearance, and to consider this disease during diagnostic processes.
12

Cechy fenotypowe i genotypowe szczepow Clostridium perfringens izolowanych od prosiat ssacych

63%
Wasinski B., Pejsak Z.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 06
791-795
The aim of present study was to determine α and β toxin expression as well as the prevalence of α, β, ε, ι, β2 toxin genes and the enterotoxin gene of Clostridium perfringens strains isolated from suckling piglets with diarrhea. Rectal swabs, feces samples and intestine sections originating from 15 farms were examined during the study. All isolated through ELISA Cl. peringen strains demonstrated β toxin expression and none of them demonstrated β toxin. All isolates were positive for the β and negative for the β, ε, ι and enterotoxin gene, implying that only non-enterotoxigenic type A strains were detected. Over 41% of the isolates demonstrated β2 toxin gene. The β2 positive strains were isolated from samples originating from 9 farms. The lack of type C strains among isolates collected during the study was also noted, as well as the different intensity of pathological changes in the intestines of piglets from which β2 positive strains were isolated.
13

Skutecznosc tiamuliny i tetracykliny w terapii zakazen mieszanych ukladu oddechowego swin

63%
Pejsak Z., Wasinski B.
Medycyna Weterynaryjna
|
1996
|
tom 52
|
nr 10
637-640
The purpose of this work was to evaluate under experimental conditions the clinical and economic of the combined use of tiamulin and tetracycline in the treatment of mixed infections of the respiratory system. Sixteen piglets, approximately 12 kg, were used in the experiment. The animals were free of Mycoplasma hyopneumoniae (M.hp.), Bordetella bronchiseptica (B.b.), Pasteurella multocida (P.m.) and Actinobacillus pleuropneumoniae (A.pp). The piglets were infected at day „0” with an M.hp. suspension at a dose of 10⁷ cfu/ml. One ml of this suspension was given into each nostril. After 14 days the animals were randomly divided into 2 groups (experimental I and control II) and infected with a suspension of dermonecrotoxic strain of P.m. The density of this culture was 10⁹ cfu/ml. Two ml of this suspension was administered into each nostril. On the same day treatment of the experimental group was started using doses of tiamulin and tetracycline recomended by the producer. After 10 days treatment was finished. Six weeks after the P.m. infection all sows were killed. The efficacy of the therapy was evaluated on the basis of bacteriological and serological examinations and post mortem findings: estimation of lesions in the lungs, isolation of M.hp. and P.m. from lung tissue. The dynamics of body weight gains and feed conversion were also determined. After investigation it was determined that administration of the above mentioned antibiotics eliminated M.hp. and P.m. in all animals from the experimental group. In the control group M.hp. was isolated in 3 cases and P.m. in 5 cases. There were considerable visible differences in the spread of lesions in lungs from treated and untreated sows. Mean body weight at the day of slaughter in the experimental group was about 6.5 kg higher than in the control. There were significant differences in dynamics of body weight gains visible in the whole period between P.m. infection and the day of slaughter. Feed conversion was also about 20% better in the treated group. It was concluded that tiamulin and tetracycline combination was effective in the therapy of experimentally induced, mixed infection of the respiratory system of pigs.
14

Fizjologiczne i patologiczne aspekty okresu noworodkowego prosiat

63%
Tarasiuk K., Wasinski B.
Przegląd Hodowlany
|
1994
|
tom 62
|
nr 09
9-11
15

Nowoczesne metody badania składu gatunkowego mięsa i produktów mięsnych

63%
Wasinski B., Osek J.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 06
Despite the permanent development of laboratory techniques, various types of adulterations are still a problem in the food industry. An important group among different frauds is adulterations connected with meat species authenticity. Uncovering of adulterated meat products is important inter alia for allergic individuals, for those who can’t intake certain species because of religious beliefs, and for maintaining fair-trade. More subtle techniques used for the mentioned adulterations generate a need for the elaboration of better analytical methods to provide effective control of meat and meat products. The aim of this review is to present currently used laboratory methods applied for meat species identification and detection of adulterations in the declared composition of meat products. The first group of the described methods enables identification and analysis of proteins and the second presented group contains techniques of DNA analysis. Apart from their short characteristics, some disadvantages and potential problems found during work with certain methods are described.
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Occurrence of leptospiral infections in swine population in Poland evaluated by ELISA and microscopic agglutination test

63%
Wasinski B., Pejsak Z.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 4
Swine are one of significant reservoirs and sources of Leptospira infections for man. Serological screenings help to effectively control the epidemiological situation in swine herds and to prevent transmission of Leptospira from animals to man. The purpose of this study was to investigate, by the use of serological methods, the prevalence of infections caused by selected Leptospira serogroups in swine population in Poland. A total of 7112 swine serum samples were examined. The samples were collected from January to October 2008 and came from 280 counties situated in all 16 provinces of Poland. All sera were examined preliminary by enzyme-linked immunosorbent assay (ELISA) using heat-stable antigenic preparation. The samples positive or doubtful in ELISA were investigated by microscopic agglutination test (MAT) with use of serovars Icterohaemorrhagiae, Pomona, Canicola, Sejroe, Tarassovi and Grippotyphosa. Of the collected sera examined by ELISA 73 (1.02%) samples were positive, 85 (1.20%) – doubtful and 6954 – negative. Among ELISA-positive and doubtful sera 64 samples (coming from 14 provinces) were recognized in MAT as positive. Among MAT positive samples 42.19% of sera demonstrated titres with serovar Pomona, 32.81% – with Sejroe, 14.06% – with Icterohaemorrhagiae, 6.25% – with Tarassovi, 3.13% – with Grippotyphosa and 1.56% with Canicola.
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Reactivity of heat-stable Leptospira antigenic preparation used in enzyme-linked immunosorbent assay for detection of antibodies in swine serum

63%
Wasinski B., Pejsak Z.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 1
Serology plays an important role in laboratory diagnosis of leptospirosis. Apart from the most often used microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA) seems to be useful especially in screenings of animal herds. The ELISA used for detection of antibodies against selected Leptospira serogroups in swine serum samples was investigated during the study. An essential element of this test is heat-stable antigenic preparation from cultures of Leptospira interrogans serovars Icterohaemorrhagiae, Pomona and L. borgpetersenii serovar Sejroe. The aim of the present study was to identify and analyze ELISA heat-stable antigen fractions playing a role in the reaction with leptospiral antibodies indicated in swine serum. Reactivity of the three-component antigenic preparation was compared in immunoblotting with reactivity of six heat-stable antigenic preparations made from the following single serovars: L. interrogans serovars Icterohaemorrhagiae, Pomona, Canicola, L. borgpetersenii serovars Sejroe, Tarassovi and L. kirshneri serovar Grippotyphosa. All antigenic preparations were submitted to SDS-PAGE and transferred to a nitrocellulose membrane using a semidry system. After the transfer, the membrane was incubated with diluted swine serum containing antibodies specific for one of the six above mentioned Leptospira serovars. For the three-component antigenic preparation and antigens prepared from single serovars the immunoblot revealed reaction of sera with fractions of the 20-26 kDa region and around the 14.5 kDa region. The investigated heat-stable Leptospira antigenic preparation contains fractions demonstrating serogroup- and species-specificity. Fraction 20-26 kDa showed serogroup-specific activity, whereas the fraction around 14.5 kDa showed species-specific activity.
18

Porownanie skutecznosci doustnego i injekcyjnego preparatu zelazowego w zapobieganiu anemii prosiat

51%
Wasinski B., Rulka J., Pawlowski R., Golembiewski Z.
Medycyna Weterynaryjna
|
1995
|
tom 51
|
nr 06
354-356
The efficacy of two iron preparations: iron-dextran-Fer- rodex and Ane-Tahna was evaluated. Ferrodex was injected i.m., into piglets at the age of 2 days whereas Ane-Tahna was applied orally to 2 and 9 day-old piglets. A single dose of the examined preparations was the equivalent of 200 mg of iron. The experiments were monitored by hematological examinations performed every week until the 49th day of life. The hematological parameters at both groups of animals were similar. It has been found that contrary to controls the treated piglets had not shown any signs of anemia. Body weight gains, morbidity and mortality of piglets treated with Ferrodex and Ane-Tahna were also similar. One can conclude that both Ferrodex and Ane-Tahna applied according to the recommendations of the producer prevent anemia in piglets.
19

Antimicrobial resistance of ESBLand AmpC-producing Escherichia coli isolated from meat

51%
Wasinski B., Rozanska H., Osek J.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 4
In the present study, 25 Escherichia coli strains isolated from beef, pork, and poultry meat, and producing extended- spectrum ß-lactamases (ESBL) (18 strains) or AmpC-cephalosporinases (7 strains) were tested for antimicrobial resistance using the minimum inhibitory concentration method with 16 antimicrobial agents. All examined strains were resistant to ampicillin and the first-generation cephalosporins. Variable resistance to the third-generation cephalosporins (40%-100% among ESBL- producing strains and 0-72% among AmpC-producing strains) was noted. Less than 30% of examined strains were resistant to ciprofloxacin. All isolates were susceptible to the fourth-generation cephalosporins, cephalosporins connected with inhibitors of ß-lactamases, carbapenems, and gentamycin.
20

Ocena przydatnosci chemioterapeutykow w leczeniu doswiadczalnie wywolanego zapalenia pluc u swin na tle infekcji mieszanych

51%
Pejsak Z., Wasinski B., Trela T., Mokrzycka A.
Medycyna Weterynaryjna
|
1995
|
tom 51
|
nr 06
333-337
The objective of the work was to determine under experimental conditions, the clinical and economical effects of mixed infections of the respiratory system in pigs, and to estimate the therapeutical efficacy of selected chemiotherapeutics in the treatment of experimentally induced pneumonia. Forty-two piglets, about 25 kg each, were used for the experiment. The animals were free of Mycoplasma hyopneu- moniae (M.hp.), Pasteurella multocida (P.m.) and Bordetella bronchiseptica (B.b.). Pigs were randomly divided into 6 experimental groups and a control group. All the animals were infected on day 0 with a M.hp. suspension at a dose of 10 cfu/ml. One ml of the suspension was instilled into each nostril. Seven days later the animals were infected in the same way with B.b. at a dose of 10 cfu/ml; 24 hr later they received a suspension of a toxinogenic strain of P.m., which was given into each nostril in a dose of 10 cfu/ml. Seven days after the application of P.m. the treatment of the animals was started by administering to each group of animals feed or water containing one of the following chemiotherapeutics: enrofloxacin (Baytril), lincomycin + spectinomycin (Linco-Spectin 100), norfloxacin (Quin-Abic), chlortetracyc- line + Tiamutin (Tetramutin) or Tiamutin and Tylan. All the drugs were administered in doses recommended by the producers for 10 days. The efficacy of infection and treatment was determined on the basis of on the results of the following examinations: (1) bacteriological examinations; (2) post-slaughter determination of quantitative pathological lesions in the particular lung lobes; (3) dynamics of body weight gains, and (4) feed conversion per kg of body weight gain. The results of these investigations were as follows: 1. Almost in all cases the experimental infection of piglets appeared to be effective; 2. The administration of the antibiotics eliminated M.hp. in all piglets treated with Tetramutin and Linco-Spectin for 10 days; 3. The best results in the elimination of B.b. infections were obtained in piglets treated with a combination of lincomycin+spectinomycin and Tetramutin; 4. A combi­nation of chlortetracycline and Tiamutin proved to be most effective in the treatment of the P.m. infections in the examined piglets. This bacterium was not found in any nasal swabs collected from piglets 3 weeks after finishing the treatment. Five weeks after infecting of the animals the body weight losses in untreated piglets were about 6 kg higher in comparison with the infected and treated animals. Feed conversion was also significantly worse: in the control group feed conversion per kg of body weight gain was 4.97 kg and in piglets treated with Tetramutin and Tiamutin it was 3.68 and 3.69 kg, respectively. Comparison of the results obtained in bacteriological and post-mortem examinations with those of body weight gains and feed conversion showed a high correlation between the healthy state of piglets and effectiveness of production.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.