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This article discusses the environmental concerns regarding soil contamination by heavy metals and the possibility of growing a high biomass-yielding crop (i.e., rapeseed) as a tool of phytoremediation. The aim of our research was to investigate the growth parameters and the capacity of rapeseed to accumulate Cd, Cu, and Zn from the contaminated soil and to investigate the effects of the chelants (EDTA, EDDS) as potential heavy metal mobility-enhancing agents. A pot experiment was performed under greenhouse conditions where rapeseed was grown on heavy metal-contaminated soil taken from former septic drain fields. Chelants were applied twice using doses of 3 mmol kg-1 of wet soil weight. Plants from contaminated soil produced more biomass and heavier seeds. The highest Cd concentrations were detected in rapeseed stems and leaves: Cu in roots and Zn in seeds and stems with leaves. Rapeseed in some cases exhibited translocation factor values for single plant parts greater than unity, whereas the bioconcentration factor was always below unity. Detected concentrations of Cd, Cu, and Zn in the biomass indicate that rapeseed may be considered an excluder rather than accumulator. Chelant application did not provide the expected enhancing effect on heavy metal uptake by rapeseed.
While it is true that heavy metals accumulate in soil easily, their removal is difficult. The organic fraction of municipal solid waste can be recycled into compost, although frequent application of biowaste may lead to the accumulation of heavy metals in soil. There is no deep-rooted tradition in Lithuania to make and use various biowaste composts, including the ones produced from municipal waste. The objective of this study has been to compare the accumulation of heavy metals in different parts of oilseed pumpkin fruit depending on the chemical composition of the compost the pumpkin plants had been supplied. The content of heavy metals (HM) was determined in the soil before the plant-growing experiment, and in biowaste composts of different origin. Green waste (GW) and municipal solid waste (MSW) composts were applied to soil as fertiliser. Heavy metal concentrations in the skin, flesh and seeds of the analysed pumpkin fruits did not exceed the maximum permissible concentrations established by the EU norms. Seeds of the investigated pumpkin cultivar Olivia are more sensitive to the effect of heavy metal accumulation than the other morphological fruit parts (skin and flesh). The highest amounts of the investigated heavy metals, except Cr, were determined in the seeds, while the lowest ones, except Cu, in the flesh of oilseed pumpkin fruits. The amount of heavy metals in pumpkin biomass was not directly related to their concentrations in the soil, which proves the fact that the transfer of heavy metals from soil to plant is determined primarily by metal bioavailability and by a plant species.
The amounts of biogenic amines (putrescine, histamine, cadaverine, tyramine, spermidine, and spermine), lactic acid, pH, and number of Enterobacteriaceae, Escherichia coli, and coagulase producing Staphylococci were determined in healthy turkeys and in the fillets of turkey breasts with the signs of ascites and bursitis. The examination of the amines was performed by high performance liquid chromatography; the amount of lactic acid was determined by capillary isotachophoresis method; pH value and microbiological indicators - by standardised methods. All determinations were done after 24, 72, and 120 h after slaughter of the turkeys. The fillets of the breasts were stored in the refrigerator at 4°C. The significant differences in the total amounts of biogenic amines in carcasses of healthy turkeys and carcasses of turkeys with substantial lesions of bursitis and medium or substantial lesions of ascites were determined within 5 d after slaughter (P<0.00l). The amounts of biogenic amines in the breast fillets of turkeys with low lesions of ascites and bursitis were very similar to that of the control group during the whole period of maturation. The average amount of lactic acid throughout 5 d after slaughter increased only in the breast fillets of healthy turkey (maximum value was 1.032 mg/100 g) and insignificantly increased in the carcasses with low lesions of ascites and bursitis (maximum value was 0.983 mg/100 g). The results of investigation indicate the breast fillets of turkeys with medium and substantial lesions of ascites and substantial lesions of bursitis are not fit for human consumption. However, in the cases of low lesions of ascites and low and medium lesions of bursitis, the fillets are not fit for maturation, but could be thermally processed in the period of 24 h after slaughter.
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