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1

Expression profiles of MGMT, p16, and APC genes in tumor and matching surgical margin from patients with oral squamous cell carcinoma

100%
Strzelczyk J. K., Golabek K., Krakowczyk L., Owczarek A. J.
Acta Biochimica Polonica
|
2016
|
tom 63
|
nr 3
2

Nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates

88%
Strzelczyk J. K., Slemp-Migiel A., Rother M., Golabek K., Wiczkowski A.
Acta Biochimica Polonica
|
2013
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tom 60
|
nr 4
 One of the mechanisms of Candida albicans resistance to azole drugs used in antifungal therapy relies on increased expression and presence of point mutations in the ERG11 gene that encodes sterol 14α demethylase (14DM), an enzyme which is the primary target for the azole class of antifungals. The aim of the study was to analyze nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates. The Candida albicans isolates represented a collection of 122 strains selected from 658 strains isolated from different biological materials. Samples were obtained from hospitalized patients. Fluconazole susceptibility was tested in vitro using a microdilution assay. Candida albicans strains used in this study consisted of two groups: 61 of the isolates were susceptible to azoles and the 61 were resistant to azoles. Four overlapping regions of the ERG11 gene of the isolates of Candida albicans strains were amplified and sequenced. The MSSCP (multitemperature single strand conformation polymorphism) method was performed to select Candida albicans samples presenting genetic differences in the ERG11 gene fragments for subsequent sequence analysis. Based on the sequencing results we managed to detect 19 substitutions of nucleotides in the ERG11 gene fragments. Sequencing revealed 4 different alterations: T495A, A530C, G622A and A945C leading to changes in the corresponding amino acid sequence: D116E, K128T, V159I and E266D. The single nucleotide changes in the ERG11 gene did not affect the sensitivity of Candida albicans strains, whereas multiple nucleotide substitutions in the ERG11 gene fragments indicated a possible relation with the increase in resistance to azole drugs.
3
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The presence of interfering antibodies in selected control sera on the example of a set for determination of tPSA

76%
Hajdrowska B., Krzakiewicz K., Czubasiewicz Z., Strzelczyk J. K., Szewczyk M., Brus R.
Health Problems of Civilization
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2015
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tom 09
|
nr 2
The human sera often contain substances which interfere in the course of immunochemical reactions. It is essential for the control sera used to determine concentrations of antigen of the prostate not to contain such ingredients. The aim of this study was to test the control sera, three producers, containing low (N) and high (P) concentrations of prostate antigen (tPSA). As a factor blocking the interfering actions, the TRU Bloc Meridian Life Science, Inc. reagent was used which contains antibodies against mouse protein and rheumatoid factor. The sera with a low concentration of tPSA demonstrated the presence of interfering substances.
4

Aktywność dysmutazy ponadtlenkowej (SOD) oraz katalazy (CAT) w gruczolakoraku jelita grubego oraz w marginesie tkanki otaczającej

76%
Strzelczyk J. K., Wielkoszynski T., Krakowczyk L., Kasperczyk J., Gawron K., Hajdrowska B., Wiczkowski A.
Diagnostyka Laboratoryjna
|
2007
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tom 43
|
nr 3
5

Selected mechanisms of molecular resistance of Candida albicans to azole drugs

76%
Golabek K., Strzelczyk J. K., Owczarek A., Cuber P., Slemp-Migiel A., Wiczkowski A.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 2
6

The levels of sICAM-1, sELAM-1, TNFα and sTNFR1 proteins in patients with colorectal adenocarcinoma in tumor and corresponding normal mucosa

64%
Strzelczyk J. K., Cuber P., Bochon B., Gajdzik K., Strzelczyk J., Krakowczyk L., Wiczkowski A., Owczarek A. J., Ostrowska Z.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 4
7

The activity of antioxidant enzymes in colorectal adenocarcinoma and corresponding normal muscoa

64%
Strzelczyk J. K., Wielkoszynski T., Krakowczyk L., Adamek B., Zalewska-Ziob M., Gawron K., Kasperczyk J., Wiczkowski A.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 4
Oxidative stress is one of several factors which contribute to the development of colorectal carcinogenesis. The aim of the study was an assessment of the activity of antioxidant enzymes in tumour and corresponding normal distal mucosa in a group of patients with colorectal adenocarcinoma. Samples of tumour and corresponding normal mucosa were obtained during a resection of colorectal cancer from 47 patients aged between 26 and 82 years. The average distance of corresponding normal distal mucosa from the tumour was 4.49 cm. Activities of antioxidant enzymes: superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferase (GST), and catalase (CAT) were measured in tissue homogenates. The patients were grouped according to the tumour stage (Duke's staging), grading, localization, and size of tumour, as well as age and sex. Statistical analysis was performed. The activity of SOD and GPx was considerably increased, while the activity of GST decreased significantly in tumour as compared with normal mucosa. GR activity in colorectal cancer was evidently higher in tumours of proximal location compared with the distal ones. The distance of corresponding normal distal mucosa from the tumour was analyzed and related to all assayed parameters. A decreased GST activity was observed in corresponding normal mucosa more than 5 cm distant from the tumour in patients with CD Duke's stage. The higher activity of superoxide dismutase and glutathione peroxidase in tumour compared to corresponding normal mucosa could indicate higher oxidative stress in colorectal adenocarcinoma cells.
8

Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer

64%
Gazdzicka J., Golabek K., Strzelczyk J. K., Kiczmer P., Miskiewicz-Orczyk K., Krakowczyk L., Ostrowska Z., Misiolek M.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 2
9

Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from Southern Poland

52%
Strzelczyk J. K., Gazdzicka J., Cuber P., Asman M., Trapp G., Golabek K., Zalewska-Ziob M., Nowak-Chmura M., Siuda K., Wiczkowski A., Solarz K.
Acta Parasitologica
|
2015
|
tom 60
|
nr 4
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