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1

Effects of copy number of an octopine synthase enhancer element and its distance from the TATA box on heterologous expression in transgenic tobacco

100%

Liu S., Bao Y.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 4

The relationship among motif copy number, its distance from the TATA box and expression level was analyzed in transformed tobacco plants. Single ormultiple copies of octopine synthase (ocs) enhancer elements from the ocs promoter were linked to the minimal ocs promoter and a β-glucuronidase (GUS) reporter gene, then transformed stably into tobacco. Reporter gene assays revealed that mere repetition of the ocs enhancer sequence is not sufficient for promoter activity. Increasing the number of copies of the ocs element elevated the level of gene expression in an additive manner that was dependent of the element’s distance fromthe TATA box. To our knowledge, this is the first report in which the regulation of transgene expression by interactions between these two factors has been documented.

2

Rosiglitazone ameliorates palmitic acid-induced endoplasmic reticulum stress and steroidogenic capacity in granulosa cells

100%

Wan J., Liu S.

Reproductive Biology

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2020

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tom 20

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nr 3

3

Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment

81%

Qin F., Wang L., Liu S., Wang Z.

Acta Ichthyologica et Piscatoria

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2013

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tom 43

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nr 2

Background. Endocrine disrupting chemicals (EDCs) are natural and anthropogenic compounds discharged into the environment known to disrupt the endocrine system of humans and animals by mimicking functions of steroids in vivo. Many important events occurring during early postembryonic development, in relation to the gene expression attracted our attention. Quantitative real-time PCR (qRT-PCR) is a sensitive and highly reproducible method for gene expression analysis, with gene expression levels quantified by normalization to reference gene. The aim of this study was to select the suitable reference gene after EDCs exposure and during early postembryonic development. Materials and Methods. For the study of the fish age effect, juveniles of Gobiocypris rarus Ye et Fu, 1983, were obtained at: 18, 22, 26, 30, 34, 38, 42, 46, and 50 days post fertilization (dpf). For mRNA expression analysis of the juvenile fish after EDCs treatment, the juveniles at 31 dpf were exposed to bisphenol A (BPA) (10 nM) and 17α-ethinylestradiol (EE2) (1 nM), respectively dissolved in dimethyl sulfoxide (DMSO) or solvent (0.001% DMSO, v/v) control group for 3 days. Cq values of the reference genes were obtained using qRT-PCR. The stability of these reference genes was analyzed by BestKeeper, geNorm, and NormFinder software, respectively. The expression of each reference gene was calculated using the 2–ΔCq method. In parallel, the mRNA expressions of cyp19a1b were normalized by the single most/least stable reference gene and the combinations of top-ranked reference genes. Results. In this study, six candidate reference genes, actb, ef1a, gapdh, g6pd, tbp, and tuba1, were chosen to analyze their expression stability in relation to fish age and in the juvenile fish exposed to BPA and EE2. During early postembryonic development of Gobiocypris rarus, actb，ef1a, and gapdh were identified as the most stably expressed reference genes. In the juvenile fish exposed to BPA and EE2 for three days, gapdh, and actb were the most stable. However, g6pd and tuba1 were identified as the least stably expressed genes during the early postembryonic development and under BPA and EE2 exposure. Conclusion. The presently reported study suggested that the mRNA expressions of the reference genes could be affected by chemical exposure or different physiological periods. In addition, it was indicated that stable reference gene should be selected to normalize the target gene expression to assure the correctness and accuracy of the experiment results. The last but not the least, we successfully obtained five commonly used reference genes of Gobiocypris rarus Ye et Fu, 1983, which can be applied in future studies serving as the stable reference gene and providing a broader range of selecting the stable reference gene.

4

Soil aggregate stability and associated structure affected by long-term fertilization for a loessial soil on the loess plateau of China

81%

Tuo D., Xu M., Li Q., Liu S.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 2

Agricultural management practices play an important role in soil structure and fertility. However, there is a lack of knowledge on the effects of long-term fertilization on aggregate structure in the Loess Plateau region in China. This study was devoted to examining the responses of soil aggregate stability and associated structure to fertilizers over 19 years under a soybean (Glycine max L.)-corn (Zea Mays) rotation. Treatments included unfertilized control (CK); nitrogen (N), phosphorus (P), and NP chemical fertilizers; and manure (M) and MN, MP and MNP organic/chemical combinations. The results showed that the water-stable aggregates, mean weight diameter, geometric mean diameter, aggregate state, and aggregate degree decreased in chemical fertilizers (N, P, and NP). However, it increased in all treatments containing manure (M, MN, MP, and MNP) compared to the CK. The changes in dispersion rate and fractal dimension were opposite to those indicators in all treatments. The applications of chemical fertilizers do guarantee an increase in crop yield, but only organic fertilizers significantly improved soil structure. These results suggest that manure’s incorporation into loessial soil is a preferred strategy for sustainable soil management.

5

Ectopic expression of PvSOC1, a homolog of SOC1 from Phyllostachys violascens, promotes flowering in Arabidopsis and rice

81%

Liu S., Ma T., Ma L., Lin X.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 07

6

Neuroprotective effects of ginsenosides

81%

Rausch W.-D., Liu S., Gille G., Radad K.

Acta Neurobiologiae Experimentalis

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2006

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tom 66

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nr 4

Ginseng, the root of the Panax species, is a well-known herbal medicine. Traditionally it has been used in Korea, China and Japan for thousands of years. Nowadays it has become a popular and worldwide known health drug. Current scientific studies demonstrate in vivo and in vitro its beneficial effects in a wide range of pathological conditions such as cardiovascular disease, cancer, immune deficiency and hepatotoxicity. Ginsenosides or ginseng saponins as the active ingredients have antioxidant, anti-inflammatory, anti-apoptotic and immunostimulant properties, which raised speculations that these compounds could positively affect neurodegenerative disorders and delay neuronal aging. Conclusive clinical data in humans are still missing. However, results from animal studies and neuronal cell culture experiments indicate that ginsenosides can counteract and attenuate factors promoting neuronal death as environmental toxins, excitotoxic action of glutamate and rises in intracellular calcium, excessive release of free radicals and apoptotic events. Thus, neuroprotective actions of ginsenosides could come about as a valuable option to slow down neurodegenerative diseases.

7

Evaluating the habitat quality of giant panda (Ailuropoda melanoleuca) using habitat suitability index (HSI)

72%

Jian J., Jiang H., Jiang Z., Zhou G., Yu S., Peng S., Liu S., Liu S., Wang J.

Polish Journal of Ecology

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2012

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tom 60

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nr 4

Habitat quality for many wildlife populations has a spatial component related to the arrangement of habitat elements across large geographic areas. With remote sensing and GIS technology, this paper presents an approach to calculate Habitat Suitability Index (HSI) for Giant Pandas to evaluate the habitat quality. In this paper, a buffer of a given distance (30 km or more) to the Giant Panda distribution area estimated in three national surveys (1974, 1989 and 2002), which is located in Sichuan, Gansu and Shanxi provinces in western China, was used as the study area. In order to study different species group’s habitat quality, the study area is divided into five parts: the Qinling mountain systems, located in the southeast in Shanxi province, the Minshan mountain systems, located in the south in Gansu province and northwest in Sichuan province, the Qionglai mountain systems, the Xiangling mountain systems and the Liangshan mountain systems, located in the west of Sichuan province, conforming to the five big Giant Panda species groups. Three physical environmental factors (elevation, slope and aspect), one ecological factor (vegetation distribution) and several human-influence factors (distances to highways, general roads, inhabitants and rural areas) are selected as the influence factors to calculate HSI. Each factor was reclassified by grid-cell (30 × 30 m per cell) to the suitability index scale from 0 to 1 based on habitat affinities before final calculation. After analyzing the HSI values on the most Giant Panda distribution area, 0.0144 was considered as the threshold habitat quality. Then, HSI was calculated for five mountain systems for three periods conforming to three national surveys (1974, 1989 and 2002). Several benefits to the approach can be highlighted. Firstly, HSI can be used as the standard to evaluate the quality of Giant Panda habitat. Secondly, by using HSI maps from 1974, 1989 and 2002, we can see that the Giant Panda habitat was the largest in 1974, and was then reduced much before 1989. However, by 2002, it had recovered to some extent, which conforms to the habitat data from the three national surveys. Thirdly, the habitat changes in the five mountain systems examined in the study are different. Finally, nature reserves play an important role in the protection of Giant Panda habitat; there are more suitable habitats in nature reserves than non-protected areas.

8

Identification and transcriptional analysis of dehydrin gene family in cucumber (Cucumis sativus)

71%

Zhou Y., Hu L., Xu S., Jiang L., Liu S.

Acta Physiologiae Plantarum

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2018

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tom 40

|

nr 08

9

Antioxidants and unsaturated fatty acids are involved in salt tolerance in peanut

71%

Liu S., Wang W., Li M., Wan S., Sui N.

Acta Physiologiae Plantarum

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2017

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tom 39

|

nr 09

10

A low-carbon generation expansion planning model considering carbon trading and green certificate transaction mechanisms

71%

Huang Y., Hu J., Yang Y., Yang L., Liu S.

Polish Journal of Environmental Studies

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2020

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tom 29

|

nr 2

11

Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa

71%

Qin L., Liang Y., Yang D., Xia G., Liu S.

Journal of Applied Genetics

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2015

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tom 56

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nr 1

12

Bindarit attenuates pain and cancer-related inflammation by influencing myeloid cells in a model of bone cancer

71%

Liu S., Gao H., Gao C., Liu W., Xing D.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

|

nr 3

13

LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53

71%

Liu S., Yue J., Du W., Han J., Zhang W.

Cellular and Molecular Biology Letters

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2018

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tom 23

Background: Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. Methods: We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosisassociated genes to identify the downstream gene(s) of LAMP3. Results: Knockdown of LAMP3 significantly inhibited OS cell viability and promoted apoptosis. TP53, which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of LAMP3. Overexpression of LAMP3 significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of TP53 partially suppressed the increased OS cell apoptosis induced by the inhibition of LAMP3, suggesting that TP53 is a key functional downstream gene of LAMP3. Conclusions: Our findings suggest that LAMP3 promotes OS cell viability and survival by regulating TP53 expression.

14

N-terminus of PutCAX2 from Puccinellia tenuiflora affects Ca2+ and Ba2+ tolerance in yeast

71%

Chen H., Wang L., Takano T., Liu S., Zhao X.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 04

15

Thallium, Arsenic, and Mercury contamination of soil near the world’s largest and longest- operating tungsten mine

71%

Cheng H., Duan X., Liu S., Lin C., Shao X.

Polish Journal of Environmental Studies

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2013

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tom 22

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nr 1

Little is known about soil thallium contamination near tungsten (W) mines. This study investigated thallium, arsenic, and mercury contents and enrichment in agricultural soils near the world’s largest and longestoperating W mine in China. Results show that the long-term W mining significantly elevated Tl from 0.77 to 1.61 mg·kg⁻¹ and As from 7.54 to 22.64 mg·kg⁻¹, with enrichment factors of 2.0 (1.4 to 3.7) and 2.9 (1.5 to 7.2), respectively. Hg contents were similar at the contaminated site (0.16 mg·kg⁻¹) and control site (0.19 mg·kg⁻¹), but were two to three times higher than the soil background content of Jianxi province. Thus, Hg contamination in the soil probably was mainly caused by coal and petrol burning instead of W mining.

16

Mitochondrial control region variability of baiji and the Yangtze finless porpoises, two sympatric small cetaceans in the Yangtze River

71%

Yang G., Liu S., Ren W., Zhou K., Wei F.

Acta Theriologica

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2003

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tom 48

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nr 4

Baiji Lipotes vexillifer (Miller, 1918) and the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis (Pilleri and Gihr, 1972) are two sympatric small cetaceans inhabiting the middle and lower reaches of the Yangtze River. In this study, a fragment (420-428 bp) of the mitochondrial control region was sequenced to provide the first comparative survey of genetic variability and population structure in these two endangered species, with samples of finless porpoises from the Yellow/Bohai Sea, East China Sea, and South China Sea also included. Low values of haplotype diversity and nucleotide diversity were found for both species, especially for the baiji and the Yangtze River and South China Sea populations of finless porpoises. The analysis of molecular variance (AMOVA) supported a high level of overall genetic structure among three porpoise populations in Chinese waters, with greatest differences found between either the Yangtze River population or the Yellow Sea population and the South China Sea population. The differentiation between the Yangtze and Yellow Sea populations was not significant, and the males have higher genetic differentiation than the females, suggesting a significant female-biased dispersal between these two populations. This study showed that the Yangtze finless porpoise, unlike the sympatric baiji, was not a genetically isolated population. The Yangtze and Yellow Sea porpoises should be included in the same management unit, but further studies using more samples and especially based on more molecular markers are urgently needed to confirm this.

17

Neurons derived from PC12 cells have the potential to develop synapses with primary neurons from rat cortex

61%

Zhou T., Xu B., Que H., Lin Q., Lv S., Liu S.

Acta Neurobiologiae Experimentalis

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2006

|

tom 66

|

nr 2

18

Physiological adaptations of Elymus dahuricus to high altitude on the Qinghai-Tibetan Plateau

61%

Cui G., Ji G., Liu S., Li B., Lian L., He W., Zhang P.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 07

The harsh natural environment on the Qinghai–Tibetan Plateau has a detrimental effect on the growth of vegetation. Elymus dahuricus, a widely distributed perennial herb on the Qinghai–Tibetan Plateau, is highly adapted to alpine regions. To unveil the mechanism of E. dahuricus adaptation to high altitude on the Qinghai–Tibetan Plateau, the relative photosynthetic characteristics and physiological indexes of wild E. dahuricus collected from different elevations in Huangzhong County and Minhe County of Qinghai Province were investigated. Increases in the maximum photochemical efficiency (Fv/Fm), potential activity (Fv/Fo), non-photochemical quenching (NPQ), total carotenoids content (Car), chlorophyll a to chlorophyll b ratio (Chl a/b) and total carotenoids to chlorophyll ratio (Car/Chl) were accompanied by decreases in photochemical quenching coefficient (qP), effective photochemical quantum yield of PSII (Y(II)) and chlorophyll a (Chl a) and chlorophyll b (Chl b) contents. Increases in Fv/Fm and Fv/F0 with altitude indicate that the photosynthetic capacity can be maintained with increases in altitude. As a photoprotective mechanism, decreases in chlorophyll content could lead to a reduction in the absorption of high energy light by leaves; therefore, the photosynthetic capacity of E. dahuricus was maintained on the Qinghai–Tibetan Plateau. Furthermore, the increasing malondialdehyde content along altitudinal gradients indicated that the alpine environments had an adverse effect on E. dahuricus growth. The increase in superoxide dismutase, peroxidase and catalase activities reflected a higher reactive oxygen species scavenging capacity, and the increases in soluble sugar and proline contents increased the osmotic adjustment substance contents, suggesting that the reactive oxygen species scavenging ability and osmotic pressure regulation play roles in maintaining the normal growth of E. dahuricus. In conclusion, the high altitude on the Qinghai–Tibetan Plateau negatively affected E. dahuricus growth, and the adaptation mechanism and survival strategies of E. dahuricus were ascribed to the comprehensive effects of photosynthetic capacity, reactive oxygen species scavenging and osmotic adjustments.

19

A simple and efficient method for obtaining transgenic soybean callus tissues

61%

Liu D., Liu S., Chang D., Wang D., Wang N. N., Wang L.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 07

In the present study, a simple and efficient method for obtaining transgenic callus tissues of soybean [Glycine max (L.) Merr.] was developed based on Agrobacterium-mediated transformation. Hypocotyl segments of soybean were used as the starting material. Several factors such as soybean genotype, Agrobacterium concentration, inoculation time, co-cultivation period and addition of antioxidants in co-cultivation medium affecting the transformation efficiency were examined. The explants were cultured on callus induction medium containing 0.5 mg L-1 6-benzylaminopurine and 2.0 mg L-1, 2,4- Dichlorophenoxyacetic acid for callus induction. Callus tissues were induced at both the acropetal and basipetal ends. CaMV35S::GUS and CaMV35S::GFP transgenic callus tissues were obtained using the optimized protocol. The average transformation efficiency reached up to 87.7 %based onGUS detection. From inoculation with Agrobacterium to obtaining transgenic soybean callus will take about 3 weeks. In order to validate this method for gene function investigation, GVG::GmSARK transgenic soybean callus tissues were obtained and their senescence-associated phenotypes were assessed. To our knowledge, this is the first report using hypocotyl segments as starting materials to obtain transgenic callus, and this system provides a method for high-throughput screening of functional genes of interest in transformed soybean callus.

20

Morphometric and pelage color variation of two sibling species of shrew (Mammalia: Soricomorpha)

61%

Chen S., Liu Y., Sun Z., Tu F., Fu C., Wang Q., Liu S.

Acta Theriologica

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2014

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tom 59

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nr 3

Pelage color variants have been documented in some small mammals, but there is not any reported about coat color variation in shrews. Here, pelage color variants of the two sibling species (Sorex cylindricauda and Sorex bedfordiae) were uncovered in different sampling sites. Our data may initiate new interest to pelage color variants in small mammals. Furthermore, the classification of two striped shrews has been controversial for several decades. We conducted a detailed examination of the morphometric characters for the two sibling shrews. Significant differences between the two species morphologically confirmed the two-species classification status.

Ograniczanie wyników

Effects of copy number of an octopine synthase enhancer element and its distance from the TATA box on heterologous expression in transgenic tobacco

Rosiglitazone ameliorates palmitic acid-induced endoplasmic reticulum stress and steroidogenic capacity in granulosa cells

Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment

Soil aggregate stability and associated structure affected by long-term fertilization for a loessial soil on the loess plateau of China

Ectopic expression of PvSOC1, a homolog of SOC1 from Phyllostachys violascens, promotes flowering in Arabidopsis and rice

Neuroprotective effects of ginsenosides

Evaluating the habitat quality of giant panda (Ailuropoda melanoleuca) using habitat suitability index (HSI)

Identification and transcriptional analysis of dehydrin gene family in cucumber (Cucumis sativus)

Antioxidants and unsaturated fatty acids are involved in salt tolerance in peanut

A low-carbon generation expansion planning model considering carbon trading and green certificate transaction mechanisms

Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa

Bindarit attenuates pain and cancer-related inflammation by influencing myeloid cells in a model of bone cancer

LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53

N-terminus of PutCAX2 from Puccinellia tenuiflora affects Ca2+ and Ba2+ tolerance in yeast

Thallium, Arsenic, and Mercury contamination of soil near the world’s largest and longest- operating tungsten mine

Mitochondrial control region variability of baiji and the Yangtze finless porpoises, two sympatric small cetaceans in the Yangtze River

Neurons derived from PC12 cells have the potential to develop synapses with primary neurons from rat cortex