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A second species of michiakii species-group of Lycocerus Gorham is described, L. strictipennis sp. nov. (CHINA, Yunnan), and provided with illustrations of aedeagus. L. michiakii Okushima et Brancucci, 2008 is recorded from China for the first time. Habitus photos of both species are presented.
The fore- and hindlimb of yak have been studied by the gross anatomical methods and standard histological techniques. The artery of ungula was also determined by X-ray and acrylonitrile-butadiene-styrene casting. Anatomical features of the forelimb of yak resemble those of hindlimb, including hoof periphery, hoof coronal, hoof wall, hoof sole, and hoof sphere. The forelimb and hindlimb are almost the same in histological structure. The epidermis comprised all 5 strata: stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale. The papillary layers and dermal lamellae contained a variable amount of capillary as well as collagen and elastic fibres. Many venules and arteriovenous anastomosis were distributed among the reticular layer. Subcutaneous tissue was composed of rich adipose and connective tissue in hoof periphery, hoof coronal, and, especially, hoof sphere. Major arteria in forelimb of yak include arteria digitalis palmaris communis III and arteriae digitales palmares propriae III and IV axialis. Those in hindlimb include arteria digitalis plantaris communis III and arteria digitalis plantaris propria III and IV axialis. Our findings highlight the main morphological features of yak and provide a morphological basis useful to researchers using yak hoof. (Folia Morphol 2016; 75, 3: 400–408)
The plant hormone jasmonic acid (JA) is critical to plant defense and plant development. Various studies have focused on the regulation of plant biotic stress by JA, but few have assessed its roles in abiotic stress. This study investigated the components of the JA signaling pathway in its response to dehydration stress. coi1-2, 35Spro:JAZ1-ΔJas and myc2-2 mutants disrupted in key regulators of JA signaling were more sensitive to dehydration stress. This suggests that JA signaling plays a key role in resistance to dehydration. Furthermore, we found that the mediation of the dehydration process by JA occurs via the induction of ERD1 expression. And our genetic data showed that ERD1 acts as a positive factor in dehydration stress. The bHLH transcription factor MYC2 could bind to the promoter of its target gene ERD1 and activate its expression to activate the JA-induced response to dehydration stress. Genetic and molecular data indicate that ERD1 functions downstream of the JA-mediated signaling pathway in response to dehydration stress. Our study, thus, reveals the following JA-mediated dehydration signaling pathway: the receptor COI1 perceives the dehydration signal, after which the signal is transmitted to MYC2, which then binds to the promoter of ERD1 and induce its expression. MYC2-induced ERD1 expression then enhances the resistance to dehydration stress. Together, our data strongly suggest a role for the JA signaling pathway in response to dehydration stress.
Grinding materials as a pretreatment contribute to successful composting. Here the grinding ratio of 4 raw materials and compost samples with different maturity degrees were compared and discussed. Results revealed that the highest grinding ratio was obtained when drying the 3 straws into constant weight, but keeping water content around 10% for sewage sludge. The carbon-to-nitrogen ratio (C/N) was an important factor impacting the grinding ratio of raw materials except water content. Different straw materials had a decreased grinding ratio from the highest to the lowest in the following order: corn stover > rice straw > wheat straw. The grinding ratio of fermented compost significantly decreased lower than about 50% when the water content was higher than 30%, whereas no significant difference was observed when the water content was lower than 20%. Compared with original materials, mature compost had a better grinding ratio. Further regression analysis implies that grinding ratio had a negative linear orrelation with total organic carbon (TOC), C/N, hemicellulose, and cellulose, but no apparent correlation with pH, EC, GI, and lignin. Results reported here may provide an efficient way to save energy and investment in the industry production of pellet compost.
As an essential micronutrient, iron (Fe) is directly involved in several fundamental processes in the photosynthetic cells. However, it is not clear if photosynthetic traits affected by high ferrous level are associated with changes in fatty acid composition in chloroplast membranes. To accomplish this, the effects of excess Fe2+ on the fatty acid composition and the fluidity properties of the chloroplast membrane, photosynthesis rate and the chlorophyll fluorescence were investigated in pea (Pisum sativum L.) seedlings grown hydroponically in nutrient solutions with 100, 200, 400 and 600 lM Fe2+ supplied as FeSO4. Increased fluidity of the chloroplast membranes was found under higher Fe2+ treatments, and this might be attributed to the increase in relative content of unsaturated fatty acids (USFA). Excess Fe2+ decreased the chlorophyll content and the electron transport rate, deactivated reaction center of photosystem II, and declined plant net photosynthetic rate. Finally, the reduced plant dry weight was observed. The results indicate that the effects of excess Fe on photosynthesis and fluidity of chloroplast membrane depend on the stress strength and duration, and the increased fluidity of chloroplast membrane may be critical in maintenance of cellular integrity under excess but not lethiferous Fe2+ treatment.
APETALA1 plays a crucial role in floral transition from vegetative to reproductive phase and in flower development. In this study, a comprehensive analysis of AP1 homologues in poplar was performed by describing the gene structure and chromosomal location. The phylogenetic relationship of the deduced amino acid sequences of Arabidopsis AP1 and AP1 homologues from Populus, to other AP1-like proteins was analyzed. The expression of PtAP1-1 and PtAP1-2 in Populus tomentosa was examined by RT-qPCR. Expression profiles were similar and both genes exhibited a high expression level in the reproductive phase. Seasonal expression profiles in floral buds indicated that the pattern of PtAP1-1 and PtAP1-2 expression in male and female floral buds was different. The trends of the PtAP1-1 and PtAP1-2 transcript levels in both sex floral buds were similar, but the peak of expression of the two genes in male buds was earlier than in female buds. This work would be of value to future functional analysis of AP1 homologues in poplar.
High floral abscission ratio in soybean (Glycine max L.) leads to serious yield loss in field culture condition. Ethylene is a phytohormone responsible for the regulation of developmental changes and floral abscission ratio in soybean. It is clear that different phytohormones affected overlapping physiological processes, and the physiological effects of phytohormone depended on specific hormone combination rather than the independent activity of each one. Little is known about how ethylene is integrated into the phytohormone metabolism and signal network. The results of phytohormone content analysis and three RNAseq libraries after silver thiosulfate (STS), ethephon (ETH) and control treatment showed that ethylene biosynthesis and signal pathway was affected by STS treatment according to suppress the mRNA abundance of 1-aminocyclopropane- 1-carboxylate (ACC) syntheses genes, while the ETH treatment induced both ethylene biosynthesis and signal pathway components in soybean. A number of genes involved in IAA, GA, CTK and ABA pathways components were activated or depressed correspondingly. The analysis of transcription factors (TFs) in differential transcriptome profiling and TFs prediction of the differentially transcription genes related to all phytohormone biosynthesis showed that TFs MYB played pivotal roles in the process of ethylene interplaying the whole phytohormones metabolism and signal network.
In this study, adventitious roots of Helicteres angustifolia L. (H. angustifolia) were generated on Murashige and Skoog salt mixture (MS) medium with auxin and ascorbic acid as additives. The root initiation ratio reached 100% on MS medium supplemented with 3.0 mg/L NAA and 0.4 mg/L ascorbic acid at 28 °C. The root number reached 15 ± 2 per leaf explant with a length of 1.70 ± 0.52 cm. Moreover, the cultured roots exhibited similar functional groups with wild plant roots and produced abundant classes of phenolic secondary metabolites. The contents of total phenolics and flavonoids of the cultured roots reached 9.68 mg GAE/g and 25.66 mg RE/g, respectively. Compared to the wild plant roots, the cultured roots produced more gallic acid (1.97 ± 0.09 mg/g), catechol (0.17 ± 0.00 mg/g), caffeic acid (0.34 ± 0.01 mg/g), and quercetin (0.84 ± 0.02 mg/g). Additionally, the adventitious root extract showed dose-dependent antioxidant capability (IC₅₀ of DPPH = 4.10 ± 0.27 mg/mL, IC₅₀ of hydroxyl = 1.59 ± 0.07 mg/mL) and inhibition activities on rat intestinal maltase and sucrase (IC₅₀ of maltase = 5.66 ± 0.20 mg/mL, IC₅₀ of sucrase > 8.0 mg/mL). The adventitious root cultures are promising alternatives to produce bioactive phenolic compounds and functional foods of H. angustifolia.
Crosstalk in wiring harness has been studied extensively for its importance in the naval ships electromagnetic compatibility field. An effective and high-efficiency method is proposed in this paper for analyzing Statistical Characteristics of crosstalk in wiring harness with random variation of position based on Polynomial Chaos Expansion (PCE). A typical 14-cable wiring harness was simulated as the object of research. Distance among interfering cable, affected cable and GND is synthesized and analyzed in both frequency domain and time domain. The model of naval ships wiring harness distribution parameter was established by utilizing Legendre orthogonal polynomials as basis functions along with prediction model of statistical characters. Detailed mean value, mean square error, probability density function and reasonable varying range of crosstalk in naval ships wiring harness are described in both time domain and frequency domain. Numerical experiment proves that the method proposed in this paper, not only has good consistency with the MC method can be applied in the naval ships EMC research field to provide theoretical support for guaranteeing safety, but also has better time-efficiency than the MC method. Therefore, the Polynomial Chaos Expansion method
To improve the efficiency of nitrogen use and to reduce the accumulation of nitrates in vegetables, an improved understanding of the mechanisms that regulate nitrate uptake and signaling is essential. Nitrogen use is regulated largely by the nitrate transporter genes, but few studies have examined the nitrate transporter genes in nonheading Chinese cabbage (Brassica rapa ssp. Chinensis Makino), one of the most important leafy vegetables in East Asia. In this study, the nitrate transporter gene BraNRT2.1 was isolated from non-heading Chinese cabbage. The cDNA for this gene contains an open reading frame of 1593 base pairs and encodes a predicted protein of 530 amino acid residues. Analysis of the BraNRT2.1 showed that BraNRT2.1 was expressed mainly in the roots and that the transcription of the gene was induced following exposure to 250 μM and 25 mM nitrate. In addition, GUS staining revealed that the BraNRT2.1 promoter directed expression to the roots. The BraNRT2.1-YFP fusion protein was observed to be localized to the plasma membrane. Finally, we observed that BraNRT2.1 could restore nitrate uptake in the presence of 200 μM nitrate in Arabidopsis thaliana plants lacking AtNRT2.1 function. Together, these results demonstrate that BraNRT2.1 encodes a high-affinity nitrate transporter that participates in nitrate uptake. These findings provide a foundation for future studies and plant breeding to improve the efficiency of nitrogen use and to reduce the accumulation of nitrates in vegetables.
We based our research on real-time monitoring data for PM2.5 at the Beijing Municipal Environmental Protection Monitoring Center of Haidian Beijing Botanical Garden (a vegetated area), and at Haidian Wanliu (a non-vegetated area). By combining these two data points with the PM2.5 and meteorological data from a separate monitoring station in Beijing Botanical Garden’s forest interior, we analyzed the daily fluctuation, regional variation, and foliar adsorption characteristics of PM2.5 in varied environments (Feb.-Dec. 2013 and Jan.-Feb. 2014). Our results show a double peak and valley pattern of PM2.5 daily variation and daytime values greater than nighttime measurements. Average annual PM2.5 concentration values at different monitoring stations were Haidian Wanliu (100.61±26.49 μg·m-3), greater than at the Beijing Botanical Garden forest interior monitoring station (89.72±23.49 μg·m-3), and both greater than at Haidian Beijing Botanical Garden (77.72±23.37 μg·m-3). The maximum PM2.5 concentrations during 12 months were all in Haidian Wanliu (non-vegetated area), while the minimums were all in Haidian Beijing Botanical Garden (vegetated), Haidian Wanliu being 83.33% of the time higher in PM2.5 concentration than Beijing Botanical Garden forest interior. Possibly because of the trees, PM2.5 concentrations in the forest area were lower than that in the non-vegetated area. We find an average PM2.5 adsorption capacity per unit leaf area of 0.048±0.031 μg·cm-2 - 0.645±0.034 μg·cm-2 in May, and 0.058±0.006 μg·cm-2 - 0.887±0.014 μg·cm-2 in June for the 10 tree species included in our study. Of these 10, incense Cedrus deodara evidences the greatest adsorption and Sophora japonica shows the minimum. As a whole, conifers adsorb at 1.32 times the rate of broadleaf tree species, according to our data. PM2.5 adsorption capacity was greater in June (0.294±0.227 μg·cm-2) than in May (0.215±0.184 μg·cm-2). Daily and annual variation in different regions falls into a pattern where Haidian Wanliu pollution is greater than Beijing Botanical Garden forest interior monitoring station, and both are greater than Haidian Beijing Botanical Garden, which confirms the forest ecosystem’s involvement. It appears that the forest has many functions, including atmospheric purification by adsorption of PM2.5 and other particulates as evidenced by better air quality in forest areas than in non-vegetated sites. Furthermore, forest clearings show better air quality than the forest interior.
Preclinical studies have suggested that increased adult neurogenesis in the hippocampus might have potential therapeutic effects for Alzheimer’s disease and depression; therefore, it is a target for the treatment of some brain diseases. In this technical communication, we propose a cell‑based fluorescence assay to study the neurogenesis of adult hippocampal progenitor cells that can be used for high‑throughput screening of drugs promoting neurogenesis. Three fluorescent dyes (DAPI, Alexa Fluor 488, and Alexa Fluor 594) and a fluorescence spectrophotometry reader were used, which confirmed that the mutual interference of the three fluorescent dyes is very low. We used this cell‑based fluorescence assay to evaluate the effects of three neurotrophic factors, ciliary neurotrophic factor (CNTF), insulin‑like growth factor 1 (IGF‑1), and IGF‑2 on the promotion of neurogenesis in adult hippocampal neural progenitor cells. The fluorescence intensity ratio of the neuronal marker, class III β‑tubulin, to the housekeeping protein, glyceraldehyde 3‑phosphate dehydrogenase, or nuclear staining dye, DAPI, in CNTF‑treated cells was significantly higher than in control cells. The ratios in IGF‑1‑ and IGF‑2‑treated cells were slightly higher under higher cell density conditions. These results are consistent with those in previous reports; therefore, this report proved the efficacy of this method. Taken together, the results showed that this simple, rapid, and economical cell‑based immunofluorescence assay could be a powerful tool for the rapid screening of drugs that promote adult neurogenesis.
The study was conducted to investigate the effects of fish oil and maize oil on nuclear factor kappa B (NFκB) gene expression and the downstream pathways of intracellular signaling in spleen of chickens after lipopolysaccharide (LPS) stimulation. Two hundred eighty eight chickens were assigned in a 2×2 factorial design. Factors were dietary fat type (4.5% maize oil or 4.5% fish oil) and immunological stimulation (LPS or saline). LPS increased levels of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) of spleen in chickens after the second LPS stimulation on 28 d of age. Fish oil alleviated the increase of EPA and DHA in spleen of chickens after LPS stimulation at 27 d of age. Fish oil supplementation decreased prostaglandin 2 (PGE2) production and the activity of cyclooxygenase 2 (COX2) after LPS stimulation. LPS stimulation increased the activity of phospholipase C (PLC) in spleen of chickens. And fish oil inhibited activity of PLC in spleen of chickens stimulated by LPS. Meanwhile fish oil decreased the production of inositol triphosphate (IP3) in spleen of chickens stimulated by LPS. Fish oil alleviated the mRNA abundance elevation of nuclear factor kappa B (NFκB) after LPS stimulation. These results showed that fish oil down-regulated the production of IP3 and PGE2 through inhibiting the activity of PLC and COX2 in spleen of chickens, respectively. The results of NFκB gene expression suggested fish oil might alleviate immune stress at the level of transcription.
The rapid global urban growth poses a great risk to the ecosystem services which are vital to sustaining and fulfilling human life. As an essential part of Fuzhou eco-planning task in south-eastern China, we identified the priority conservation areas for ecosystem services to allow a sustainable urban expansion. We modeled and mapped five ecosystem services (carbon storage, timber production, water yield, water-quality improvement and sediment retention) using InVEST and proposed a definition of priority areas for the conservation of ecosystem services. Priority areas for individual ecosystem services showed that 24% of the region was needed to produce 26% of water yield. Between 6 and 17% of the region was required to conserve at least 20% of other four services, depending on the ecosystem service of interest. In addition, scenarios for multiple ecosystem services conservation were developed using the ordered weighted averaging (OWA) method, a multicriteria evaluation method, to take the tradeoffs among ecosystem services into account. The results showed that, along with the decrease of the OWA risk, the overall areas and the areas at both of the conservative levels dropped gradually. Furthermore, two efficiency indices were created to evaluate the performance of different OWA scenarios. Study results suggested that the efficiency of scenarios was associated with the conservation threshold and OWA risk, as well as the spatial correlation among ecosystem services. In sum, identifying priority areas for ecosystem services in a spatially explicit manner, and analyzing tradeoffs between them, can help make land use and natural resource decisions more effective and efficient.
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