Wyniki wyszukiwania

1

Jak korzystac z prognoz pogody?

100%

Koziel R.

Gazeta Obserwatora IMGW

|

1995

|

tom 44

|

nr 5

7-9

2

Przeplywy i powiazania migracyjne Wroclawia

84%

Huk J., Koziel R.

Czasopismo Geograficzne

|

1995

|

tom 66

|

nr 3-4

331-347

3

Effect of ciprofloxacin on mitochondrial energy metabolism, calcium signaling and viability of Jurkat T-cells

68%

Koziel R., Zablocki K., Duszynski J.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

4

Ciprofloxacin inhibits proliferation and promotes generation of aneuploidy in Jurkat cells

51%

Koziel R., Szczepanowska J., Magalska A., Piwocka K., Duszynski J., Zablocki K.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 2

Ciprofloxacin is widely used in antimicrobial therapy. However it also inhibits mitochondrial topoisomerase II and therefore affects cellular energy metabolism. At a concentration exceeding 80 µg/ml ciprofloxacin induces apoptosis, while at 25 µg/ml it inhibits proliferation of Jurkat cells without any symptoms of cell death. The aim of this study was to explain the mechanisms of ciprofloxacin-evoked perturbations of the cell cycle. Human lymphoidal cells (Jurkat) were exposed to ciprofloxacin (25 µg/ml) for 4-11 days and effects of the drug on cell proliferation (light microscopy), cell cycle (flow cytometry), cell size and morphology (confocal microscopy) as well as number of chromosomes (chromosomal spread analysis) were investigated. Exposition of Jurkat cells to ciprofloxacin inhibited cell proliferation, increased proportion of cells in the G2/M-phase of the cell cycle, compromised formation of the mitotic spindle and induced aneuploidy. These observations indicate that ciprofloxacin applied at concentrations insufficient for induction of apoptosis may stop cell proliferation by inhibition of mitosis. Chromosomal instability of such cells may, at least potentially, increase a risk of cancer development.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Czasopismo Geograficzne

1 Gazeta Obserwatora IMGW

1 Journal of Physiology and Pharmacology

4 Koziel R.

2 Duszynski J.

2 Zablocki K.

1 Huk J.

1 Magalska A.

1 Piwocka K.

1 Szczepanowska J.

1 2010

1 2003

2 1995

Jak korzystac z prognoz pogody?

Przeplywy i powiazania migracyjne Wroclawia

Effect of ciprofloxacin on mitochondrial energy metabolism, calcium signaling and viability of Jurkat T-cells

Ciprofloxacin inhibits proliferation and promotes generation of aneuploidy in Jurkat cells