Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 14

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Medial septal NMDA receptor inhibition increases time spent in open arms in high and low responder rats submitted to the elevated plus-maze model of anxiety

100%
Podlacha M., Grzybowska M., Plucinska K., Wrona D.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: The limbic glutamatergic neurotransmission may be involved in the biological mechanisms underlying anxiety-related disorders. The purpose of the present study was to determine the influence of NMDA glutamatergic receptor antagonist – D-AP7 infusions into the medial septum (MS) on time spent in open arms in rats differing in behavioral characteristics, stress susceptibility and anxiety level measured by their locomotor response to novelty: high (HRs) or low (LRs) responders under the elevated plus maze (EPM) test paradigm which is a rodent model of anxiety. METHODS: Male Wistar rats prior categorized as HRs or LRs in the novelty test (2 h) were exposed to the EPM test (5 min) in the baseline and 15 min after injection of D-AP7 (DL-2-amino-7- phosphoheptanoate, receptor antagonist; 0.1 µg/rat in 0.5 µl saline solution; n=15) or saline (0.5 µl/rat; n=12) via implanted cannulae into the MS. Data are presented as mean±SD. RESULTS: Following the D-AP7 injection, a significant increase in time spent in the open arms in both HRs (46±6 s) and LRs (102±6 s) within the D-AP7 group, in comparison with the baseline value (HR: 19±9 s, LR: 11±5 s; P≤0.001) and the SAL control group (HR: 24±9 s, LR: 19±9 s; P≤0.001), was observed. In the LRs, time in the open arms was significantly longer (LR: 102±6 s), as compared to the HRs (HR: 46±6 s; P≤0.001). CONCLUSIONS: The obtained results indicate that blocking of MS NMDA glutamate receptors decreases the expression of anxiety – like behavior, indicating by increased time spent in open arms in rats. This effect is more pronounced in rats with higher anxiety level but lower behavioral activity and stress susceptibility, which are attributed to the low responders. This study was supported by Gdańsk University grant for young scientists from Poland: 538-L124-B597-14.
2

Influence of glutamate injection into unilateral nucleus accumbens shell on behavioral response elicited by ipsilateral stimulation of the mesolimbic system

100%
Plucinska K., Jerzemowska G., Podlacha M.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Food intake is regulated not only by homeostatic requirements but also by emotional factors. The nucleus accumbens, particularly its shell region (AcbSh) is a part of the mesolimbic dopaminergic system which is responsible for a positive emotional aspect of various homeostasis-relevant stimuli. In the present work, we tested the AcbSh involvement in feeding behavior using an experimental paradigm specifically designed to assess motivational vs. motor aspect of food ingestion. METHODS: In rats (n=4), feeding was evoked by electrical stimulation of the midbrain ventral tegmental area (a somatodendritic region of mesolimbic system) and assessed quantitatively with the use of the latency to feed/stimulation frequency curve shift paradigm before and after ipsilateral glutamate injection (dose 2.0 µg dissolved in 0.5 µl of distillated water) into AcbSh (distillated water injection as a control, volume: 0.5 µl). RESULTS: Effect of ipsilateral glutamate injection into the AcbSh on behavioral response following the VTA stimulation was varied. In three rats the percentage reaction threshold did not change significantly and was approximated the baseline (not exceed ±10%). We observed an increase/decrease in the reaction threshold by only +0.31%, +2.85% and −1.90% in comparison to the water injection. In one rat the feeding threshold reaction was changed by significantly more than 10%. This significant increase was about +19.40% as compared to the baseline (water injection). CONCLUSIONS: Glutamate AcbSh activation – the major terminal area of the mesolimbic system does not affect the behavior induced by stimulation of the dopaminergic cells at the level of VTA. Presumably a different effect observed in one rat is dependent on the injected place within the AcbSh area. Research was financed by the Polish National Science Centre (NCN); decision no: DEC-2013/09/N/NZ4/02195
3

Medial septal NMDA receptor inhibition affects pro-inflammatory response in rats differing in their stress susceptibility

100%
Podlacha M., Myslinska I., Wrona D., Wegrzyn G.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
4

Effects of electrical stimulation of the central nucleus of the amygdala on stress-related behaviors and plasma corticosterone level in rats

76%
Rucinski J., Czerwiec K., Wadolowska A., Kurowska E., Podlacha M., Myslinska D., Jurkowlaniec E.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: The central nucleus of the amygdala (CeA) has efferent projections to regions involved in autonomic, endocrine, and behavioral responses to emotional stimuli. Due to its role in the regulation of the activity of corticosterone releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus (PVN), the CeA is considered a key modulator of behavioral responses to stress. Examine how electrical stimulation of the CeA, which is known to participate in the integration of sensory and emotional informations with neuroendocrine responses, influences stress-related behaviors and plasma corticosterone level in rats. METHODS: Male Wistar rats implanted under standard stereotaxic surgery with stimulating electrodes into the CeA were divided into the following groups: CeA 14–day electrical stimulation (n=12) and CeA sham (n=10) – control, no stimulation. Behavioral activity during the 5-min testing period in the elevated plus maze (EPM) was recorded and analyzed (e.g. number of entries into closed arms, time spent in open arms). Additionally episodes of grooming were measured. Blood samples were collected by heart puncture (isoflurane anesthesia) seven days before (basal), one hour and seven days after the last stimulation. The plasma corticosterone level (CORT) was determined by radioimmunoassay. RESULTS: Stress-related activity in the EPM and grooming episodes were increased in stimulated rats after last stimulation. Whereas the control group showed gradual adaptation to the testing conditions which was manifested by decreased behavioral activity. Effects of CeA stimulation were also confirmed by an increase in plasma corticosterone level. CONCLUSIONS: Electrical stimulation of the CeA induces stressrelated behavior which is reflected by active avoidance of stressful situation (escape behavior) and adaptive activities to stressful conditions (grooming). Research was supported by The National Science Centre, grant number: NN303819040.
5

Subthalamic nucleus deep brain stimulation influence on erythrocyte number and hemoglobin levels in a rat model of early Parkinson's disease

76%
Grembecka B., Koriat P., Podlacha M., Majkutewicz I., Ptaszek K., Plucinska K., Wrona D.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Subthalamic nucleus deep brain stimulation (STN-DBS) is most effective treatment for Parkinson’s disease (PD) motor symptoms. A number of epidemiological studies have recently highlighted the association between hemoglobin (HGB) levels and PD risk. Interestingly, several lines of evidence confirm that STN-DBS increases regional cerebral blood flow and oxygen concentrations in target brain areas. AIM(S): Considering the close association between oxygen concentration, red blood number (RBC), and HBG, we hypothesized that enhanced blood flow during STN-DBS may influence peripheral RBC parameters in a rat model of early PD. METHOD(S): Male Wistar rats were implanted unilaterally for STN-DBS and received intranigral (substantia nigra pars compacta, SNpc) infusion of 6‑OHDA. After recovery, rats were subjected to STN-DBS for 7 days (1h daily, n=6) or SHAM stimulation (control, n=6). Immediately after collection, peripheral blood samples were analyzed using automated hematology analyzer (Cell Dyn 3700). The RBC number, hematocrit percentage (HCT), HGB concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were measured. PD model was verified by the detection of tyrosine hydroxylase positive neurons in SNpc. For a statistical analysis of the results, SPSS 22.0 software was used. RESULTS: The Student’s t‑test showed that STN‑DBS rats had a significantly higher number of RBC in comparison to the SHAM rats (t(10)=‑2.912; p≤0.05). The HCT percentage slightly increased but differences did not reach statistical significance. Mann‑Whitney U tests showed that HGB level was higher in STN-DBS rats (Z=‑1.290; p≤0.05). CONCLUSIONS: The STN-DBS applied in a rat model of early PD has an influence on RBC number and HGB level. The obtained results suggest that there are peripheral compensation mechanisms for the increased oxygen demand during STN‑DBS in rats. FINANCIAL SUPPORT: Department of Animal and Human Physiology fund.
6

Dimethyl fumarate alleviates reference memory impairment and modulates brainderived neurothropic factor expression in streptozotocin-induced rat model Alzheimer's disease

76%
Kurowska E., Majkutewicz I., Myslinska D., Grembecka B., Grzybowska M., Podlacha M., Rucinski J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Intracerebroventricular (icv) injection ofstreptozotocin (STZ) induces brain glucose hypometabolism, memory impairment, progressive cholinergic deficit, activation of microglia, oxidative stress and neurodegeneration. It is used as an animal model of sporadic form of Alzheimer’s disease (AD). The aim of this study was to determine if dimethyl fumarate (DMF), oral anti-oxidative and immunosuppressive drug, alleviates spatial reference memory impairments in STZ-icv induced rat model of AD. Additionally, the expression of brain derived neurotrophic factor (BDNF) was measured. METHODS: There were four experimental groups: STZ DMF (n=8) – STZ-icv infused and fed with 0.4% DMF fodder for three weeks until spatial memory test of Morris, STZ CTR (n=8) – STZ-icv infused and fed with standard fodder, and VEH DMF and VEH CTR groups (n=10) – vehicle-icv infused and fed with 0.4% DMF or standard fodder, respectively. A three-day Morris water maze test (four trials per day with unchanged platform location) and the probe test on the fourth day were performed. Rats were sacrificed and brain subjected to immunofluorescent BDNF labeling. RESULTS: The latency to reach the platform in the second and third day of testing was significantly longer in the STZ CTR rats than in the remaining groups, which showed tendency to reduce the latency day by day. STZ DMF rats did not differ in the results of the spatial memory test of Morris from control VEH CTR and VEH DMF groups. All STZ rats showed reduced BDNF expression in the hippocampus, but in the hypothalamus STZ DMF showed more BDNF+ cells than STZ CTR rats. CONCLUSION: Oral medication with DMF alleviates spatial reference memory impaired after STZ-icv infusion. The decrease of BDNF expression after STZ-icv infusion was prevented by DMF in the hypothalamus. The study was financed by the National Science Centre Poland on the basis of decision DEC-2013/09/D/NZ4/01658.
7

Streptozotocin and dimenthyl fumarate decreases plasma tumor necrosis factor Alpha concentration in rats

76%
Grzybowska M., Podlacha M., Majkutewicz I., Kurowska E., Myslinska D., Grembecka B., Wrona D.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: This study aims to determine if dimethyl fumarate (DMF), antioxidant having immunosuppressive properties, taken orally for three weeks will affect plasma tumor necrosis factor  alpha (TNF-α) concentration in an animal model of sporadic form of Alzheimer’s disease (sAD) induced by intracerebroventricular (icv) injection of betacytotoxic drug, streptozotocin (STZ). METHODS: Blood samples from young, male Wistar rats divided into four groups: STZ DMF (subjected to icv injection of STZ, fed with 0.4% DMF fodder), VEH DMF (subjected to icv injection of vehicle, fed with 0.4% DMF fodder), STZ CTR (subjected to icv injection of STZ, fed with standard fodder), VEH CTR (subjected to icv injection of vehicle, fed with standard fodder) were taken one hour after Morris water maze test finishing. Then, TNF-α concentration was determined with ELISA method using a Rat TNF-α ELISA Kit. RESULTS: Injections of STZ in rats being on the control feed (STZ/ CTR) significantly decreased (P≤0.05) the  plasma concentration of  TNF-α (22±2 pg/ml; mean±SE) as compared to the controls (VEH/CTR: 33±3 pg/ml). Moreover, within the STZ/DMF group, a significant (P≤0.01) decrease in the concentration of  TNF-α (22±0.8 pg/ml)  as compared to the controls  (VEH/DMF: 30±2 pg/ ml), was observed. CONCLUSION: The obtained results indicate that streptozotocin injection and feeding with dimethyl fumarate of the streptozotocininduced sAD rats reduce such a peripheral blood pro-inflammatory cytokine level as TNF-α.
8

Dimethyl fumarate prevents spatial working memory impairment and does not affect brain IL-6 expression in streptozotocininducted rat model of Alzheimer's disease

76%
Majkutewicz I., Kurowska E., Myslinska D., Grembecka B., Grzybowska M., Podlacha M., Rucinski J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Animal model of sporadic form of Alzheimer Disease (AD) evoked by intracerebroventricular (icv) injection of betacytotoxic drug, streptozotocin (STZ), reflects memory impairments, brain hypometabolism, cholinergic deficit, activation of microglia and neurodegeneration found in AD patients. Brain inflammation is important factor contributing to exacerbation of AD symptoms, but some studies show neuroprotective properties of pro-inflammatory cytokine IL-6. The aim of this study was to determine if dimethyl fumarate (DMF), which has anti-oxidative and immunosuppressive properties, can alleviate spatial working memory impairments in STZ-icv induced rat model of AD and change the expression of IL-6 in the brains. METHODS: Four experimental groups were separated: STZ DMF (n=8) – STZ-icv infused and fed with 0.4% DMF fodder for three weeks until spatial memory test of Morris, STZ CTR (n=8) – STZicv infused and fed with standard fodder, and VEH DMF and VEH CTR groups (n=10) – vehicle-icv infused and fed with 0.4% DMF or standard fodder, respectively. Morris water maze testing was performed for three days, with four trials per day with unchanged platform location, and rats were then sacrificed and brains subjected to immunofluorescent IL-6 labeling. RESULTS: The latency to reach the platform in each trial was significantly longer in the STZ CTR rats than in the remaining groups. STZ CTR was the only group which did not decrease the latency and the distance swum to platform in the consecutive trials. STZicv infused rats  (STZ CTR and STZ DMF groups) had also lower number of the IL-6 expressing cells in the hippocampus and the hypothalamus than control VEH CTR rats. CONCLUSION: Oral medication with DMF prevents spatial working memory impairment evoked by STZ-icv infusion, but has no influence on the central expression of IL-6. The study was financed by the National Science Centre Poland on the basis of decision DEC-2013/09/D/NZ4/01658.
9

NMDA receptor modulation of the pedunculopontine tegmental nucleus decreases the number of tyrosine hydroxylase positive cells in the ventral tegmental area and substantia nigra pars compacta in rats

76%
Plucinska K., Piwka A., Ptaszek K., Podlacha M., Orzel-Gryglewska J., Jerzemowska G.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Two notable targets of the pedunculopontine tegmental nucleus (PPN) circuitry, the substantia nigra (SN) and the ventral tegmental area (VTA), are implicated in locomotion and reward processing. A dysfunction of these regions occurs in Parkinson’s and related disorders as well as in various psychiatric conditions, and over the course of normal aging AIM(S): In the present study, we were interested in understanding NMDA-receptors involvement in the interactions between the PPN and SN/VTA midbrain complex. In order to obtain more insight into this process, we analyzed the number and the distribution of midbrain tyrosine hydroxylase positive cells (TH+). METHOD(S): All rats were implanted with bilateral stimulating electrodes in the VTA and with bilateral guide cannulas for intracerebral injections into the PPN. Immunohistochemistry for TH+ was used to measure the number of active dopaminergic neurons in midbrain (VTA-SN) of rats subjected to unilateral VTA electrical stimulation and local injection of MK‑801 (5 μg) or NMDA (3 μg) to the contralateral or ipsilateral hemispheres into the PPN (4 experimental groups). The control brains were from rats in which only the 14‑day unilateral electrical VTA‑stimulation was performed (control group). RESULTS: Immunohistochemical analysis revealed a decrease in the number of TH+ cells in the midbrain. When the main subdivisions of the VTA/SN were subjected to a separate analysis, a significantly lower number of TH+ cells were found in all experimental groups in the PBP (parabrachial pigmented nucleus), PB (paranigral nucleus) and SNc (SN, pars compacta), as compared to the control group. CONCLUSIONS: The level of NMDA receptor arousal in the PPN regulates the activity of the midbrain dopaminergic cells. FINANCIAL SUPPORT: The research was funded by the Polish National Science Center; decision no: DEC‑2013/09/N/NZ4/02195 and by the Faculty of Biology, University of Gdansk, Poland.
10

Genistein-mediated lysosomal degradation of main patogenic factors of Alzheimer’s disease as a novel therapeutic strategy

76%
Pierzynowska K., Podlacha M., Gaffke L., Majkutewicz I., Mantej J., Myslinska D., Wegrzyn G.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
11

Brain-derived neurotropic factor (RDNF) expression and behavioral response during stimulation of bed nucleus of the stria terminalis (BST) in rats

64%
Myslinska D., Majkutewicz I., Podlacha M., Ciepielewski Z., Karnia M., Wadolowska A., Rucinski J., Siudak M., Zajaczkowski S., Wrona D.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUNDANDAIMS: The bed nucleus of the stria terminalis (BST) is a part of “the extended amygdala”, a formation responsible for emotional aspects of behavior. The BST is considered as a site of convergence of information from brain regions associated with the control of emotional, cognitive, autonomic and behavioral responsesto stress and noxiousstimuli. On the basis of our previousstudy we assumed that the BST also influenced the primary antitumor immune response. In the present study we investigated the influence of 14-day electrical stimulation of the BST on the brain-derived neurotrophic factor (BDNF) expression and behavioral response. METHODS: Male Wistar rats implanted with electrodes into the BST were divided into groups: BST stimulated and BST sham. The intensity of stimulation current (120–160 µA; 50 Hz) was determined individually for each stimulated rat to induce a behavioral response such a locomotor reaction. The current intensity was raised incrementally in 30 s trials (30 trials/day, 20 s rest between the trials). Behavioral reaction was measured in the Opto Varimex Minor actometer during stimulation procedure. BDNF was detected during immunofluorescence procedure. RESULTS: The stimulation of the BST caused induction of BDNF expression in brain cortical and subcortical motor structures: the frontal primary motor cortex (areas FR1 and FR2), prefrontal cortex, ventral tagmental area, as well as in the central amygdala nucleus and in the hypothalamus: the paraventricular and the supraoptic nuclei, the medial preoptic and the lateral areas positively correlated with the augmentation of the behavioral activity which appeared as locomotor activity (increase in the average number of movements in horizontal and vertical plane). CONCLUSION: This suggests that the behavioral outcome of the BST stimulation, imitating physical exercise, could be responsible for brain BDNF synthesis observed in the study. Supported by: NN303819040.
12

Behavioral responses to amygdaloid stimulation

64%
Myslinska D., Rocinski J., Czerwiec K., Wadolowska A., Ciepielewski Z., Kurowska E., Majkutewicz L., Plucinska K., Podlacha M., Ptaszek K.
Acta Neurobiologiae Experimentalis
|
2014
|
tom 74
|
nr 3
The central nucleus of the amygdala (CeA) is involved in fear conditioning and participates in euroendocrine response to stressful stimuli. In our previous study, we found that CeA also influences immune function, especially innate antitumore response. In the present study, we investigated the influence of 14-day electrical stimulation of the CeA on corticosterone level (CORT; determined by radioimmunoassay) and behavioral response: locomotor activity in the new environment (measured in the Opto Varimex Minor actometer), explorative and anxiety-like behavior [assessed in the openfield (OF) and in the elevated plus-maze (EPM) tests]. Male Wistar rats implanted with stimulating electrodes into the CeA were divided into groups: CeA 14-day electrical stimulation (n=20) and CeA sham (n=18). Current intensity (70–120 µA; 50 Hz) was raised incrementally in 30-s trials until behavioral reaction in the Opto Varimex Minor actometer was observed. OF and EPM tests were performed after termination of stimuation procedure. Electrical stimulation of the CeA caused augmentation of CORT level (P<0.001) correlated with an increase in the average number of movements in horizontal and vertical plane imitating escape behavior. These effects were accompanied by an increase in anxiety-related behavior in the EPM and decreased locomotor activity and exploration in the OF. We suggest the hormonal response to of the CeA stimulation plays a crucial role in the regulation of the behavioral response. Supported by The National Science Centre, grant number: NN303819040.
13

Stimulation of the bed nucleus of the stria terminalis and the medial septal nucleus influences natural killer cell cytotoxicity of rats differing in responsiveness to novelty

64%
Myslinska D., Plucinska K., Glac W., Grembecka B., Listowska M., Podlacha M., Ptaszek K., Wrona D.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
In our previous study we found that electrolytic lesion of the bed nucleus of the stria terminalis (BST) as well as the medial septal nucleus (MS) caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) in rats. In the present study we evaluated blood NKCC after 14 day electrical stimulation of the BST and the MS in conscious, freely behaving rats differing in responsiveness to novelty. Male Wistar rats divided into high (HR) and low (LR) responders to novelty, implanted with stimulating electrodes at the BST or at the MS, were subjected to 14 day electrical stimulation (constant current 0.1 ms duration cathodal pulses delivered at a frequency of 50 Hz during 30 min) of the BST and the MS. The chronic stimulation of the BST and the MS caused augmentation of blood NKCC in comparison to the sham operated group and to the baseline, which was more significant in HRs. A week after termination of the stimulation procedure NKCC returned to the baseline. The obtained results suggest that immunoenhancing effect on blood NK cell function is dependent on the behavioral outcome (intensive locomotor reaction) of the BST and the MS stimulation as well as on individual behavioral characteristics. This work was supported by a research grant NN303819040.
14

Proces autofagii indukowany genisteiną jako nowe podejście w leczeniu chorób neurodegeneracyjnych

64%
Pierzynowka K., Podlacha M., Myslinska D., Majkutewicz I., Mantej J., Niedzialek N., Wegrzyn A., Wegrzyn G.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.