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Biosystematics studies on the greens used by local inhabitants of Pothamalai Hills In, Namakkal District, Tamil Nadu, India

100%

Sathiyaraj G., Kumuthakalavalli R.

International Letters of Natural Sciences

2014

tom 11

nr 1

The present study mainly focuses on the ethno-botanical importance of greens, used by the Pothamalai, Puduppatti, Vadugam, Malaiyampatti, Seerappalli and Rasipuram local people in Namakkal districts. From this investigation 25 species of green belonging to19 genera and 13 families are recorded. This information regarding on correct botanical identity with family, vernacular name, systematic description and medicinal uses was observed. During the survey brought out some popular medicinal plants frequently used by the local villagers for minor ailments such as felt by the respondents were greens are good for health, increase the blood level, clear vision, to cure sore in the stomach and mouth, prevent the jaundice, cure the heart diseases, release the knees pain, and sugar disorder. The greens are good food for children and give essential nutrient for pregnant women.

Molecular characterization of a cysteine proteinase inhibitor, PgCPI, from Panax ginseng C. A. Meyer

51%

Jung D. Y., Lee O. R., Kim Y.-J., Lee J. L., Pulla R. K., Sathiyaraj G., Shim J. S., Yang D. C.

Acta Physiologiae Plantarum

2010

tom 32

nr 5

Phytocystatins are plant-derived cysteine proteinase inhibitors implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cysteine proteinase inhibitor, PgCPI, was isolated and characterized from Panax ginseng. Sequence analysis revealed that the coding cDNA sequence of PgCPI is 609 base pairs in length with a predicted molecular mass of 22.5 kDa. A GenBank BlastX search revealed that the deduced amino acid sequence of PgCPI shares a high-degree homology with cysteine proteinase inhibitors from other plants. In this present study, we analyzed the expression patterns of PgCPI against various abiotic and biotic stresses at different time points using quantitative real time-PCR. Enzymatic activity of PgCPI was also determined against cysteine proteinase. Our results reveal that PgCPI is moderately induced by NaCl, chilling, CuSO₄, ABA, and jasmonic acid. However, high light, UV, MeJA, and wounding triggered a significant induction (more than fourfold) of PgCPI within 12-h post-treatment, especially PgCPI prominently accumulated by wounding (24-fold). In addition, increased transcripts of PgCPI were investigated in fungal- and nematode-infected roots. These results suggest that PgCPI is involved in defense responses to biotic and abiotic stresses.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 International Letters of Natural Sciences

2 Sathiyaraj G.

1 Jung D. Y.

1 Kim Y.-J.

1 Kumuthakalavalli R.

1 Lee J. L.

1 Lee O. R.

1 Pulla R. K.

1 Shim J. S.

1 Yang D. C.

1 2014

1 2010

Wyniki wyszukiwania

Biosystematics studies on the greens used by local inhabitants of Pothamalai Hills In, Namakkal District, Tamil Nadu, India

Molecular characterization of a cysteine proteinase inhibitor, PgCPI, from Panax ginseng C. A. Meyer