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1

Scanning electron microscopy study of infective juveniles Heterorhabditis bacteriophora [Nematoda: Heterorhabditidae]

100%
Skrzypek H., Kazimierczak W., Kreft A.
Annales Universitatis Mariae Curie-Sklodowska. Sectio C, Biologia
|
2004
|
tom 59
2

Effectiveness of infection of Cameraria ohridella [Lepidoptera: Gracillariidae] larvae by entomopathogenic nematodes Steinernema sp. and Heterorhabditis sp. [Nematoda: Rhabditida]

100%
Kreft A., Skrzypek H., Kazimierczak W.
Annales Universitatis Mariae Curie-Sklodowska. Sectio C, Biologia
|
2004
|
tom 59
3

Efektywność porażania larw chrabąszcza majowego (Melolontha melolontha L.) przez entomopatogeniczne nicienie

88%
Kreft A., Skrzypek H., Kazimierczak W., Lech-Pituch E., Skrabucha A.
Progress in Plant Protection
|
2011
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tom 51
|
nr 2
Experiments done and presented below aimed at proving that applied species and Polish strains of entomopathogenic nematodes (EPNs) can be used to reduce the number of Melolontha melolontha. Invasive larvae of Steinernema arenarium Bys, S. arenarium Lub, S. feltiae Rap, Heterorhabditis bacteriophora Sie, H. megidis Pla, H. megidis Lub i H. megidis Pla, H. megidis C3 were used in the experiments as biocontrol agents against the larvae L1, L2 and L3 of M. melolontha. The experiments were done at 20°C, in 25 ml boxes filled with pasteurized (3 h at 70°) and air-dried soil. Pathogen doses were 100 IJ/insect. The result showed that susceptibility of M. melolontha to EPNs depends on the insect's developmental stage. Larvae L1 were the most susceptible. S. arenarium Bys and H. megidis strains were more effective in infecting M. melolontha larvae than S. feltiae Rap and H. bacteriohora Sie.
4
Content available remote

Mechanisms involved in the development of the small intestine mucosal layer in postnatal piglets

76%
Skrzypek T. H., Kazimierczak W., Skrzypek H., Valverde Piedra J. L., Godlewski M. M., Zabielski R.
Journal of Physiology and Pharmacology
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2018
|
tom 69
|
nr 1
5

Characterization of nematodes isolated from cockchafer Melolontha sp. (Coleoptera: Scarabaeidae) grubs

76%
Kreft A., Kazimierczak W., Sierpinska A., Skrzypek H., Skrabucha A., Dybala M., Dzwierzynska M.
Progress in Plant Protection
|
2012
|
tom 52
|
nr 4
The main aim of the research was characterization of nematode species isolated from Melolontha sp. larvae. European cockchafer grubs were collected from the forest territory of Krasnystaw, Kozienice, Opoczno, Brzeziny, from areas with different light conditions, humidity and temperatures. The isolated nematodes were described based on light and scanning electron microscopy, supplemented with SSU rDNA (ribosomal deoxyribonucleic acid) sequence data. The obtained results revealed that grubs were infected by the following nematode species: Diplogasteroides magnus, Koerneria sp. and Rhabditis sp. Most of the grubs infected by nematodes were collected on tree-covered areas, where the surface was shadowed and humidity was the highest.
6
Content available remote

Gradual disappearance of vacuolated enterocytes in the small intestine of neonatal piglets

76%
Skrzypek T., Valverde Piedra J. L., Skrzypek H., Kazimierczak W., Biernat M., Zabielski R.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
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nr 3
87-95
7

The contribution of vacuolated foetal-type enterocytes in the process of maturation of the small intestine in piglets Invited review

76%
Skrzypek T., Szymanczyk S., Ferenc K., Kazimierczak W., Szczepaniak K., Zabielski R.
Journal of Animal and Feed Sciences
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2018
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tom 27
|
nr 3
In neonates the vacuolated foetal-type enterocytes (VFE) play a key role in the transport of intact colostral and milk proteins from the lumen of the small intestine into the circulation and/or in the intracellular digestion of intestinal nutrients. The absorption of intact colostral macromolecules (including immunoglobulins, hormones and bioactive peptides) is important in the development of the immune and digestive systems of newborn piglets. The digestion of the intestinal content inside the VFE supports the luminal digestion of nutrients. The presence of apical canalicular system, which produces both the transport and the digestive vacuoles, is a key feature of VFE. The VFEs are gradually replaced by adult-type enterocytes. VFEs disappear gradually from the proximal part of the small intestine to the ileum. VFEs containing large (also referred to as giant) transport vacuoles disappear within the first 2–3 days after birth. VFEs containing digestive vacuoles are present for up to week 3 of life. In contrast, VFEs of intrauterine growth retarded piglets show abnormalities in their development of the apical area. The loss of VFEs is a good marker of the small intestine epithelium maturation.
8

Rozwoj jelita cienkiego u prosiat karmionych mlekiem matki i preparatem mlekozastepczym w pierwszym tygodniu zycia

76%
Skrzypek T., Valverde Piedra J. L., Skrzypek H., Kazimierczak W., Wolinski J., Zabielski R.
Folia Universitatis Agriculturae Stetinensis. Zootechnica
|
2006
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tom 48
67-80
9
Content available remote

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of gram-positive and gram-negative bacteria to the gut epithelium - in vitro study

64%
Jankowska A., Wrzesinski M., Laubitz D., Kazimierczak W., Skrzypek H., Bardowski J., Zabielski R., Grzesiuk E.
Journal of Physiology and Pharmacology
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2008
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tom 59
|
nr 4
795-810
The adhesion of six different Lactobacillus and Lactococcus and three pathogenic Escherichia and Salmonella strains was studied using Caco-2 cell line. In this in vitro model system the influence of weak electric field (EF) on bacterial adhesion was tested. The EF source was the in vitro reconstruction of spiking potentials recorded in the duodenum of a healthy calf during one myoelectrical migration complex (MMC) cycle. The ability to adhere to Caco-2 cells of bacteria belonging to two groups, Gram-positive lactobacilli and lactococci, and Gram-negative Escherichia and Salmonella differed considerably. The pathogenic bacteria adhered better to well-differentiated Caco-2 cells whereas lactobacilli and lactococci displayed better adhesion to non-differentiated Caco-2 cells. In the presence of MMC-related EF an increased adhesion of Lactobacillus and Lactococcus but not of Salmonella enterica s. Enteritidis and E. coli 269 to Caco-2 cells was observed. Two later strains adhered even less in the presence of EF. The same tendency was found in the presence of pancreatic juice in a cell medium. In conclusion, the myoelectric component of the small intestinal motility, the MMC-related EF, and pancreatic juice may increase the ability of lactic acid bacteria to adhere to GI epithelial cells, creating better environmental conditions for colonization of the intestine and competition with Gram-negative pathogens.
10
Content available remote

Light and scanning electron microscopy evaluation of the postnatal small intestinal mucosa development in pigs

64%
Skrzypek T., Valverde Piedra J. L., Skrzypek H., Wolinski J., Kazimierczak W., Szymanczyk S., Pawlowska M., Zabielski R.
Journal of Physiology and Pharmacology. Supplement
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2005
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tom 56
|
nr 3
71-87
Modifications in the structure of gastrointestinal mucosa is often used to evaluate gut function for instance during the development or in response to particular food components. Scanning electron microscopy (SEM) gives a chance to observe the surface of the gut epithelium in three dimensions. However, this technique is seldom used due to technical difficulties. The present study attempted to investigate the intestinal mucosa structure changes in the postnatal pig using light and scanning electron microscopy technique. Experiments were carried out on sow reared piglets from birth until 38 days of age. Piglets were sacrificed at birth and at the 3rd, 7th, 21st and 38th day of life. The entire gastrointestinal tract was immediately harvested and the whole thickness tissue samples were taken from the duodenum, jejunum and ileum for optical and scanning electron microscopy. SEM analyses corroborated with histometry made by optical microscopy. Moreover, a number of shape modifications of the villi and its surface have been observed. The development changes in small intestine mucosa during the first 3 weeks were manifested in shape, size and density of villi. In conclusion, the structure of small intestinal mucosa undergoes profound structural changes. SEM gives a new dimension in the investigation of gut mucosa.
11

Inwazja glist Baylisascaris transfuga u niedźwiedzia brunatnego (Ursus arctos) - opis przypadku

64%
Szczepaniak K., Tomczuk K., Listos P., Lopuszynski W., Skrzypek T., Kazimierczak W., Lojszczyk-Szczepaniak A., Lisiak B.
Życie Weterynaryjne
|
2014
|
tom 89
|
nr 05
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