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1

Identification of microRNAs potentially involved in male sterility of Brassica campestris ssp. chinensis using microRNA array and quantitative RT-PCR assays

100%
Jiang J., Jiang J., Yang Y., Cao J.
Cellular and Molecular Biology Letters
|
2013
|
tom 18
|
nr 3
microRNAs (miRNAs) are a class of newly identified, noncoding, small RNA molecules that negatively regulate gene expression. Many miRNAs are reportedly involved in plant growth, development and stress response processes. However, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. Pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. This study aimed to identify miRNAs involved in pollen development. A microarray assay was conducted using the known complementary sequences of plant miRNAs as probes on inflorescences of a sterile male line (Bcajh97-01A) and a fertile male line (Bcajh97-01B) of the Brassica campestris ssp. chinensis cv. ‘Aijiaohuang’ genic male sterility sister line system (Bcajh97-01A/B). The results showed that 44 miRNAs were differently expressed in the two lines. Of these, 15 had over 1.5-fold changes in their transcript levels, with 9 upregulated and 6 downregulated miRNAs in inflorescences of ‘Bcajh97-01A’ sterile line plants. We then focused on 3 of these 15 miRNAs (miR158, miR168 and miR172). Through computational methods, 13 family members were predicted for these 3 miRNAs and 22 genes were predicted to be their candidate target genes. By using 5’ modified RACE, 2 target genes of miR168 and 5 target genes of miR172 were identified. Then, qRT-PCR was applied to verify the existence and expression patterns of the 3 miRNAs in the flower buds at five developmental stages. The results were generally consistent with those of the microarray. Thus, this study may give a valuable clue for further exploring the miRNA group that may function during pollen development.
2

Fatty acid composition and PlFADs expression related to alpha-linolenic acid biosynthesis in herbaceous peony (Paeonia lactiflora Pall.)

72%
Meng J., Jiang J., Tao J.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 10
3
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Seed vigor, photosynthesis and early growth of saplings of different triploid Betula families

72%
Mu H., Jiang J., Li H., Liu G.
Dendrobiology
|
2012
|
tom 68
Breeding scientists have given extensive attention to triploids in trees because of their importance to forestry. Consequently, creating and breeding triploids of good quality has become one of purposes of tree breeding. We chose two autotetraploids (Betula platyphylla, named Q10 and Q65) as female parents and eight hybrid diploids (B. platyphylla × B. pendula, named F1 – F8) as male parents to obtain progenies through controllable pollination, resulting in triploid progenies. Germination rate and germination energy of triploid seeds of Q65 were significantly higher (P < 0.01) than in triploid seed s of Q10. Triploid families with Q65 as female parent had a large quantity of saplings, whereas triploid families with Q10 as female parent had a small quantity of saplings. Triploid families with Q65 as female parent were generally superior in base diameter and height to base diameter ratio when compared to a diploid family. Q65×F3 was preliminarily recognized as the superior family. These results demonstrate that the female parent has a major influence on triploid progenies, although the male parent also has a small influence. The results provided a reference to build seed orchards of triploid birch trees, choose tetraploids as female parents and forecast triploid families of good quality.
4

Interspecies competition between microcystis aeruginosa and Scenedesmus obliquus under phenanthrene stress

72%
Jin X., Jiang J., Sheng L., Jin M.
Polish Journal of Environmental Studies
|
2014
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tom 23
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nr 5
Interspecies competition is considered an important effector of community structure in ecosystems. Interspecies interactions may change due to changing environmental factors, including exogenous organic pollutants. In this paper, we measured the change in population density, based on the logistical growth model, of Microcystis aeruginosa and Scenedesmus obliquus when they were cultivated in single-species and mixedspecies cultures, under the stress of the polycyclic aromatic hydrocarbon (PAH) phenanthrene. The singlespecies culture experiment showed that S. obliquus could tolerate greater phenanthrene stress than could M. aeruginosa, and exhibited hormesis when the concentration of phenanthrene was 0.0625 mg/l. In the mixedspecies culture experiment, the toxicity of phenanthrene on the two algae changed. In the 0.0625 mg/l and 0.25 mg/l treatments, the population density of S. obliquus increased, whereas the population density of M. aeruginosa in each group decreased. Finally, the influence of different phenanthrene concentrations on the interspecies competition was evaluated.
5

Association of urine lead exposure with lipid metabolism risk factors and diseases in Xinxiang, China

63%
Dong X., Wang Y., Jiang J., Li Z., Wu W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
6

3-D simulation of vertical-axial tidal current turbine

63%
Zhang Z., Ma Y., Jiang J., Liu W., Ma Q.
Polish Maritime Research
|
2016
|
tom 23
|
nr 4
Vertical-axial tidal current turbine is the key for the energy converter, which has the advantages of simple structure, adaptability to flow and uncomplex convection device. It has become the hot point for research and application recently. At present, the study on the hydrodynamic performance of vertical-axial tidal current turbine is almost on 2-D numerical simulation, without the consideration of 3-D effect. CFD (Computational Fluid Dynamics) method and blade optimal control technique are used to improve accuracy in the prediction of tidal current turbine hydrodynamic performance. Numerical simulation of vertical-axial tidal current turbine is validated. Fixed and variable deflection angle turbine are comparatively studied to analysis the influence of 3-D effect and the character of fluid field and pressure field. The method, put the plate on the end of blade, of reduce the energy loss caused by 3-D effect is proposed. The 3-D CFD numerical model of vertical-axial tidal current turbine hydrodynamic performance in this study may provide theoretical, methodical and technical reference for the optimal design of turbine
7
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Characteristics of the staminate flower and pollen from autotetraploid Betula platyphylla

63%
Lin L., Yao Q., Xu H., Mu H., Jiang J.
Dendrobiology
|
2013
|
tom 69
Comparison of the staminate flower and pollen of Betula platyphylla between autotetraploid and diploid individuals may reveal the reason for cross sterility when an autotetraploid male parent is crossed with a diploid female parent. Staminate flowers and pollen from seven autotetraploid individual trees were examined. The morphologies of the staminate flower and pollen and pollen germination of these autotetraploid individuals were compared with diploid trees. The structure of the staminate flower did not differ between autotetraploids and diploids, whereas the size of the staminate flower was larger in the autotetraploids compared with the diploids. The mean diameter and pore depth of the pollen were significantly larger in autotetraploid individuals compared with diploids, and several abnormalities in pollen morphology were found commonly amongst pollen from the autotetraploid trees. The germination rate of pollen from autotetraploids was significantly inferior to that of diploids and there was a significant negative correlation between germination rate and the percentage of abnormal pollen. These results demonstrate that the autotetraploid should not be chosen as the male parent to obtain hybrid triploid progenies because the pollen is abnormal and results in low fertility despite its large size. The results provide important information for selecting and distributing parent strains with the aim of building seed orchards of triploid birch trees.
8

Specific role of the tight junction proteins occludin and claudin-5 on the blood–brain barrier during Listeria monocytogenes infection

63%
Ren J., Yang M., Wang P., Jiang J., Yan G.
Medycyna Weterynaryjna
|
2020
|
tom 76
|
nr 06
To investigate the blood-brain barrier (BBB) permeability of mice after Listeria monocytogenes infection for further study on the mechanism of L. monocytogenes crossing the BBB, a mouse model was established and Evans blue assay was performed to assess the BBB disruption. Using relative quantitative real-time PCR, the RNA expression of Zonula occludens-1 (ZO-1), occludin and claudin-5 were detected. In addition, the protein expression level of ZO-1, occludin and claudin-5 were detected by immunohistochemistry and western blot. The extravasation of Evans blue dye was significantly different between 24 h and 96 h (P < 0.05). The mRNA expression of occludin and claudin-5 were down-regulated than that of the control group at each sampling point (P < 0.05) and ZO-1 showed a significant change at 96 h (P < 0.05). In addition, the protein expression level of occludin and claudin-5 decreased significantly at 48 h and 96 h (P < 0.05) by immunohistochemistry and western blot, compared with the control, while ZO-1 was almost unchanged (P > 0.05). All results indicating that the tight junction integrity of endothelial cells was destroyed and BBB permeability was enhanced in the process of L. monocytogenes infection, and this change was related to the decrease of the expression occludin and claudin-5.
9
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Wpływ wstrząsów sejsmicznych na przyrost radialny i anatomię drewna wybranych gatunków drzew (Podhale, Polska i Wenchuan, Chiny)

54%
Gawior D., Wistuba M., Malik I., Tie Y., Michalowicz P., Jiang J.
Studia i Materiały Centrum Edukacji Przyrodniczo-Leśnej
|
2016
|
tom 18
|
nr 3[48]
10

Molecular cloning and expression analysis of a squalene synthase gene from a medicinal plant, Euphorbia pekinensis Rupr.

54%
Zheng Z., Cao X., Li C., Chen Y., Yuan B., Xu Y., Jiang J.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 10
Euphorbia pekinensis Rupr., which is also known as a medicinal plant, produces a large amount of alkaloids, phytosterols and triterpenes. In this study, we reported on the cDNA cloning and characterization of a novel squalene synthase (SQS) from E. pekinensis. Squalene synthase catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to produce squalene (SQ), the first committed precursor for sterol and triterpene biosynthesis. The full length cDNA named EpSQS (Genbank Accession Number JX509735) contained 1,614 bp with an open reading frame of 1,236 bp encoding a polypeptide of 411 amino acids. The deduced amino acid sequence of the EpSQS named EpSQS exhibited a high homology with other plant SQSs, and contained a single domain surrounded by helices. Phylogenetic analysis showed that EpSQS belonged to the plant SQS kingdom. Tissue expression analysis revealed that EpSQS expressed strongly in roots, weakly in stems and leaves, implying that EpSQS was a constitutive expression gene. The recombinant protein was expressed in Escherichia coli and detected by SDS-PAGE and western blot. The high performance liquid chromatography (HPLC) analysis showed that EpSQS could catalyze the reaction from farnesyl diphosphate (FPP) to squalene.
11
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Ocena zagrożenia osuwaniem na postawie analizy przyrostów rocznych olszy nepalskiej (Syczuan, Chiny)

54%
Malik I., Tie Y., Wistuba M., Jiang J., Owczarek P., Hapczyk M.
Studia i Materiały Centrum Edukacji Przyrodniczo-Leśnej
|
2016
|
tom 18
|
nr 3[48]
12

Functional distribution of bacterial community under different land use patterns based on FaProTax function prediction

54%
Liang S., Deng J., Jiang J., Wu S., Zhou J., Zhu W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 2
13

Improvement of bioactive compound accumulation in adventitious root cultures of an endangered plant species, Oplopanax elatus

54%
Jiang X.-L., Piao X.-C., Gao R., Jin M.-Y., Jiang J., Jin X.-H., Lian M.-L.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 10
14

Comparison of physiological and methylational changes in resynthesized Brassica napus and diploid progenitors under drought stress

54%
Jiang J., Yuan Y., Zhu S., Fang T., Ran L., Wu J., Wang Y.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 04
Brassica napus is a polyploid of certain research and economical value. Resynthesizing B. napus with diploid B. rapa and B. oleracea is essential for Brassica research because of the limited genetic background of B. napus. Considering that polyploids possess better agronomic traits and resistance compared with the corresponding diploids, we investigated drought tolerance after polyploidization of B. napus and revealed the epigenetic differences between polyploids and diploids. After drought stress, B. rapa and first-generation of synthesized hybrids (F1) were more wilted than B. oleracea and F2–F4 generations. However, the relative water content and water retention in F1 were better than others after drought stress. The increased number of partially opened and closed stomata in F1 was not as significant as that in F2 and F3, but stomata density in F1 was lower than F2, and the stomatal size in F1 was significantly reduced than F3. Physiological parameters varied among different generations of B. napus and diploid parents, and most of these parameters in hybrids were higher than B. rapa and lower than B. oleracea. However, the peroxidase activity in F3 and F4 was significantly higher than both parents, and the malondialdehyde content in F3 and F4 was lower than both parents, indicating that F3 and F4 might be more adaptive to oxidative stresses than other generations. DNA methylation level was decreased in F2 and F3 compared with F1, and then increased in F4. Methylation-sensitive amplified polymorphism analysis revealed that DNA methylation and demethylation broadly happened after drought stress. The methylation and demethylation level was F1 > F4 > B. oleracea > F2 > F3 > B. rapa and B. rapa > F4 > F3 > F2 > B. oleracea > F1, respectively. The epigenetic changes under drought stress might be related to the different stress tolerances during B. napus polyploidization.
15

Cell suspension culture of Orostachys cartilaginous in bioreactor systems for bioactive compound production and evaluation of their antioxidant properties

54%
Piao X.-C., Zhang W.-B., Jiang J., Jin Y.-H., Park P.-J., Kim S.-E., Lian M.-L.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 03
16

Cloning and expression of Perilla frutescens FAD2 gene and polymorphism analysis among cultivars

54%
Xue Y., Zhang X., Wang R., Chen B., Jiang J., Win A. N., Chai Y.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 03
17

Examination of molecular mechanism for the color mutation in Chinese wild grapevine (Vitis davidii)

54%
Niu S., Hao F., Mo H., Jiang J., Liu C., Wang H.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 08
18

Molecular cloning and functional analysis of an organ-specific expressing gene coding for farnesyl diphosphate synthase from Michelia chapensis Dandy

54%
Yin T., Cao X., Miao Q., Li C., Chen X., Zhou M., Jiang J.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 1
Farnesyl diphosphate synthase (FPS; EC 2.5.1.1/EC 2.5.1.10) catalyzes the synthesis of farnesyl diphosphate, a key intermediate in the biosynthesis of sesquiterpenes. This present study described the cloning and characterization of a cDNA encoding FPS from leaves of Michelia chapensis Dandy (designated as McFPS, GenBank accession number: GQ214406) for the first time. McFPS was 1,432 bp and contained an open reading frame (ORF) of 1,056 bp, encoding a protein of 351 amino acids with a calculated molecular mass of 40.52 kDa. Bioinformatic analysis revealed that the deduced McFPS had high homology with FPSs from other plant species. Phylogenetic tree analysis indicated that McFPS belonged to the plant FPS group and had the closest relationship with FPS from Chimonanthus praecox. Southern blot analysis revealed that there were at most two copies of McFPS gene existed in M. chapensis genome. The organ expression pattern analysis showed that McFPS expressed strongly only in leaves, and there were no expression in stems and roots, implying that McFPS was an organ-specific expressing gene. Functional complementation of McFPS in a FPS-deficient yeast strain demonstrated that cloned cDNA encoded a farnesyl diphosphate synthase.
19

Transgenic poplar ‘‘NL895’’ expressing CpFATB gene shows enhanced tolerance to drought stress

54%
Zhang L., Liu M., Qiao G., Jiang J., Jiang Y., Zhuo R.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 02
Acyl-ACP thioesterases are responsible for the export of fatty acids produced by the de novo fatty acid synthesis system from the plastid. A fatty acyl–acyl carrier protein thioesterase (CpFATB) was isolated from a Chimonanthus praecox (linn.) Link. (wintersweet) cDNA library. This gene was subsequently transferred into Populus deltoides CL 9 P. euramericana CL ‘‘NL895’’ through Agrobacterium tumefaciens-mediated leaf disc transformation. The transgenic plants were confirmed by polymerase chain reaction (PCR) analysis and Southern hybridization. Three positive transgenic lines each with a single T-DNA insertion were obtained. Reverse transcription PCR (RT-PCR) confirmed CpFATB expression in transformed plants after short-term exposure to drought. When exposed to drought stress, transgenic plants showed higher superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, chlorophyll a content, chlorophyll b content and total chlorophyll content compared with untransformed plants. Malondialdehyde (MDA) content, electric conductivity and net photosynthetic rate of the transgenic plants were lower than untransgenic plants. The results of physiological indices showed that the overexpression of CpFATB in plants exhibited higher drought stress tolerance under drought stress condition compared to the untransformed control plants. Our data further confirmed and highlighted the functions of CpFATB in enhancing plants drought tolerance and may provide a plausible approach to breed plants combating drought stress and enlarging living scope.
20

Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress

54%
Fang Y., Li J., Jiang J., Geng Y., Wang J., Wang Y.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 06
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