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1

Theaflavin-3, 3′- digallate attenuates rheumatoid inflammation in mice through the nuclear factor-kappaB and MAPK pathways

100%

Liu W., Li J.

Archivum Immunologiae et Therapiae Experimentalis

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2019

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tom 67

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nr 3

2

Experiment and modeling of soil-water characteristic curve of unsaturated soil in collapsing erosion area

81%

Liu W., Luo X., Fu M., Huang J.

Polish Journal of Environmental Studies

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2016

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tom 25

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nr 6

Soil erosion tends to occur with rainfall runoff, thus leading to grave soil and water loss. An increase of water content in soil caused by rain makes the loss of matrix suction and the decrease of shear strength obvious, and will promote soil erosion. The soil-water characteristic curve (SWCC) can be used to describe the relationship between the water content and the matrix suction in unsaturated soil. For this paper we studied the SWCCs of the granite residual soils in a collapsing erosion area in Jiangxi Province, China. A GEO-Experts pressure plate extractor was used to measure SWCCs for soils with different dry density, grain size, drying and wetting cycles, and lime content. The initial dry density has a significant impact on SWCC. With increasing dry density, the suction was decreased for the same water content. The larger the grain size, the greater the suction value for the same volumetric water content. Under the same suction, the volumetric water content decreases as the lime percentage increases and water stability improves. SWCCs of the drying and wetting cycle demonstrate the hysteresis phenomenon. The area of the hysteresis loop decreased with the increase of the dry density and drying and wetting cycle number. It also became small when the soils were mixed with lime. In this paper, the Van Genuchten model, the Fredlund and Xing model, and the Gardner model were used to fit the experimental data of SWCCs. The presented fitting parameters show that the residual sum of squares is less than 0.002. All the experimental data fit well to three models for SWCC. The results indicated that the simulated value of the Gardner model does provide best agreement with the measured value. These results will provide an important basis for the further study of the soil collapsing erosion process and soil cover design.

3

VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer

81%

Chen Y., Zhang L., Liu W., Wang K.

Cellular and Molecular Biology Letters

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2018

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tom 23

Background: Anti-angiogenesis therapy that targets VEGF is one of the important treatment strategies in advanced ovarian cancer. However, depending on the pharmaceutical agent, treatment can have undesirable side effects. SEMA4D has recently gained interest for its role in promoting angiogenesis. Here, we try to further understand the mechanism by which SEMA4D promotes angiogenesis in ovarian cancer. Methods: Correlation and western blot assaya were used to detect the relationship between VEGF and SEMA4D in clinical tissues and cells. Vasculogenic mimicry and transwell migration analyses were used to detect the roles of VEGF, SEMA4D and plexin-B1 on vasculogenic mimicry and migration. Vascular density and SEMA4D expression was determined using immunofluorescence staining in clinical tissues of EOC. Western blot was used to detect the expressions of CD31, MMP2 and VE-cadherin. We also analyzed the relationship between VEGF-SEMA4D and malignant tumor prognosis. Results: We found that knockdown of VEGF could suppress SEMA4D expression and that the expressions of VEGF and SEMA4D have a positive correlation in EOC cancer tissues. Vasculogenic mimicry and transwell migration analyses showed that SEMA4D and VEGF have a synergistic effect on the promotion of angiogenesis in A2780 and HUVEC cells. Soluble SEMA4D (sSEMA4D) could promote VM and migration in A2780 and HUVEC cells via the SEMA4D/plexin-B1 pathway, but the effect was not noted in stably transfected shR-plexin-B1 cells. In clinical tissues of EOC, the vascular density and SEMA4D/plexin-B1 expression were higher. When VEGF, SEMA4D and plexin-B1 was knocked down, the expression of CD31, MMP2 and VE-cadherin, which are the markers and initiators of angiogenesis and the epithelial–mesenchymal transition (EMT) process were reduced. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. Conclusions: VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer. Targeting VEGF and the SEMA4D signaling pathway could be important for the therapy for EOC.

4

Effect of including different levels of palm kernel cake to dairy cow concentrate on milk production and composition

81%

Jin S. G., Li M., Liu W., Zhou Y.

Journal of Animal and Feed Sciences. Supplement

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2007

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tom 16

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nr 2

5

Winter food availability limits winter survival of Mongolian gerbils (Meriones unguiculatus)

81%

Liu W., Wang G., Wan X., Zhong W.

Acta Theriologica

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2011

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tom 56

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nr 3

Food availability is important to the dynamics of animal social organizations or populations. However, the role of winter food availability in animal population dynamics is still controversial. We carried out an experimental study to test Lack’s hypothesis that reduced food in winter limits survival and spring numbers of breeding individuals of social groups, using the Mongolian gerbil (Meriones unguiculatus) as model species. We established 24 gerbil social groups in 24, 10 × 10 m, pens in September 2008. We provided wheat seeds as supplemental food in 12 enclosures from September 2008 to March 2009; the other 12 enclosures, not provided with supplemental food, served as controls. We live-trapped gerbils at a 2-week interval from September to April. Supplemental food during winter increased biweekly survival by 10% relative to that in control groups. Only four control social groups survived to the end of our study whereas all 12 food-supplemented social groups survived through our study period. Supplemental food also increased cumulative numbers of recruits and group sizes of gerbils. We conclude that winter food availability limits winter survival and spring social groups or population sizes of Mongolian gerbils.

6

Activated carbon prepared from semi-coke as an effective adsorbent for dyes

81%

Dong M., Zhou H., Liu W., He C.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 2

7

The port service ecosystem research based on the Lotka-Volterra model

81%

Li W., Liu W., Xu X., Gao Z.

Polish Maritime Research

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2017

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tom 24

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nr Special Issue S3

Under the new normal of China’s economy, the competition among the port enterprises is not only the competition of the core competence of the port, the port industry chain or the port supply chain, but also the competition of the port service ecosystem. In this paper, the concept and characteristics of the port service ecosystem is discussed, a hierarchical model of the port service ecosystem is constructed. As an extended logistic model, Lotka-Volterra model is applied to study the competitive co-evolution and mutually beneficial co-evolution of enterprises in the port service ecosystem. This paper simulates the co-evolution of enterprises in the port service ecosystem by using MATLAB programming. The simulation results show that the breadth of the niche of the enterprises is changing with the change of the competition coefficient and the coefficient of mutual benefit in the port service ecosystem. Based on that, some proposals are put forward to ensure the healthy and orderly development of the port service ecosystem

8

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A review of progress in current research on Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)

81%

Liu W., Liu Y., Kleiber T.

Journal of Elementology

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2021

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tom 26

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nr 1

9

Overexpression of Rosa roxburghii L-galactono-1,4-lactone dehydrogenase in tobacco plant enhances ascorbate accumulation and abiotic stress tolerance

81%

Liu W., An H.-M., Yang M.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 05

L-Galactono-1, 4-lactone dehydrogenase (GalLDH; EC 1.3.2.3) is the last key enzyme in the putative L-ascorbic acid (AsA) biosynthetic pathway of higher plants. To evaluate the effect of the gene on manipulating AsA accumulation, a cDNA encoding GalLDH(RrGalLDH, Acc. No. AY643403), isolated from Rosa roxburghii fruit known to be rich in AsA, was introduced into tobacco plants by Agrobacterium-mediated transformation under CaMV 35S constitutive promoter in the present study. Southern blotting revealed the stable integration of the transgene with single copy in four independent transgenic lines, among which, L3 and L4 showed the significantly enhanced RrGalLDH transcript levels, GalLDH activities and AsA accumulations as compared to untransformed (WT) plants. So, we developed the AsA-overproducing tobacco plant by overexpressing GalLDH. As exposed to salt stress (100 mM NaCl), these AsA-overproducing transgenic lines were found to grow better with increased shoot length and fresh weight than WT. Furthermore, L3, which demonstrated the highest AsA accumulation (2.1-fold higher than WT) and expression level of RrGalLDH, showed a higher resistance to oxidative stress caused by paraquat when compared to WT. These results further justify that the overexpression of GalLDH gene confers an elevated AsA accumulation and tolerance against environmental stresses.

10

A PSBM model for environmental efficiency evaluation and its application

81%

Song M., Peng J., Liu W., An Q.

Polish Journal of Environmental Studies

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2014

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tom 23

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nr 3

The traditional efficiency evaluation model considering undesirable outputs is only able to calculate the efficiency of the overall value of the decision-making units. This paper puts forward a new environmental efficiency evaluation based on the proper slack-based measurement (PSBM) model, which can give the efficiency values of the inputs, desirable outputs, and undesirable outputs. The results from the new model are not only highly correlated with SBM-based evaluation results, but also coincide with reality, which can be used to test the effectiveness of the new model. Our model provides more information for environmental efficiency evaluation analysis.

11

Identification and characterization of differentially expressed genes from tobacco roots after decapitation

81%

Qi Y., Ma L., Wang F., Liu W.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 2

Nicotine is the major alkaloid in tobacco and its biosynthesis is regulated by a variety of factors. Topping, as an important agronomic factor, can induce the nicotine biosynthesis increase. Some key nicotine biosynthesisrelated genes and the framework of nicotine biosynthesis pathway have been well studied, but the details of nicotine biosynthesis pathway are not well understood now. To investigate the genes expressed after tobacco topping, we constructed a suppression subtractive hybridization library using cDNA from control tobacco plants as driver and those from topped tobacco plants as tester. The insert size of positive clones was 200–1,000 bp confirmed by PCR. After differential screening, 560 significantly differently expressed clones among 1,950 positive clones were acquired, sequenced and 273 high quality expressed sequence tags (ESTs) were acquired. The results of nucleotide blast homological analysis indicated that these ESTs mainly involved in alkaloid biosynthesis (4%), plant hormone metabolism (3%), signaling/transcription (18%), stress/ defense (32%), protein metabolism (9%), carbon metabolism (6%), other metabolism (15%) and function unknown (13%). The expression of selected genes was analyzed by reverse transcription polymerase chain reaction and RNA gel blot hybridization, and the result indicated that their transcription amount increased after tobacco topping. NtNAC-R1was in silico cloned, and the expression level of NtNAC-R1 increased at 12 and 24 h in tobacco roots after topping, which indicated that NtNAC-R1 may play an important role in the signal transduction after tobacco topping. In addition to many previously reported nicotine biosynthesis-related genes, some new genes, such as transcription factors related to nicotine biosynthesis/regulation and the members of plant hormone pathway, were discovered in our library. The results contribute new data to the list of possible candidate genes involved in nicotine biosynthesis and regulation.

12

Quantitative analysis of the relationship between shear strength and fractal dimension of solidified dredger fill with different fly ash content under monotonic shear

81%

Yang S., Liu W., Liu H.

Polish Maritime Research

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2018

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tom 25

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nr Special Issue S2

The dredger fill of Shanghai Hengsha Island Dongtan is solidified by curing agents with different fly ash content, and the shear strength index of solidified dredger fill is measured by the direct shear test. The microscopic images of solidified dredger fill are obtained by using SEM. The microscopic images are processed and analyzed by using IPP, and the fractal dimension including particle size fractal dimension Dps, aperture fractal dimension Dbs and particle surface fractal dimension Dpr is calculated by fractal theory. The quantitative analysis of the relationship between shear strength index and fractal dimension of solidified dredger fill is done. The research results show that the internal friction angle and the cohesion are closely related to the fly ash content λ and the curing period T, and the addition of fly ash can improve the effect of curing agent; There is no obvious linear relationship between the internal friction angle and the three fractal dimensions; The smaller particle surface fractal dimension Dpr and particle size fractal dimension Dps, the larger aperture fractal dimension Dbs, the greater the cohesion, and the cohesion has a good linear relationship with three fractal dimensions, and the correlation coefficient R2 is above 0.91

13

Variation and correlations of leaf traits of two Taxus species with different shade tolerance along the light gradient

81%

Liu W., Li S., Su L., Su J.

Polish Journal of Ecology

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2013

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tom 61

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nr 2

The variations in leaf traits of Taxus species in different light conditions are still poorly understood. We sampled leaves of Taxus yunnanensis W. C. Chang L. K. Fu and Taxus chinensis var. mairei (Lemée and Lév.) W. C. Cheng and L. K. Fu along an illumination gradient (full daylight, 40–60% full daylight, <10% full daylight), and analyzed how seven leaf traits and their correlations changed under different light conditions. The leaf trait that showed the greatest variation was specific leaf area (SLA) for T. yunnanensis, followed by leaf dry mass (LM) for both taxa. The smallest variation was in leaf width (LW) for both taxa. Plasticities of all leaf traits in both taxa were higher than 50%, and those of leaf area (LA), LM and SLA were greater than 80%. The light gradient was positively correlated with leaf length (LL), LM, leaf dry matter content (LDMC), and leaf length to width ratio (LWR). LW and SLA were negatively correlated with the light gradient. Analyses of relationships among leaf traits showed that LM of T. yunnanensis, T. chinensis var. mairei and both taxa was positively correlated with LL, LW, LA, LDMC, and LWR, and negatively correlated with SLA under all light conditions. We concluded that leaf traits and their relationships were affected by light conditions.

14

3-D simulation of vertical-axial tidal current turbine

71%

Zhang Z., Ma Y., Jiang J., Liu W., Ma Q.

Polish Maritime Research

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2016

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tom 23

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nr 4

Vertical-axial tidal current turbine is the key for the energy converter, which has the advantages of simple structure, adaptability to flow and uncomplex convection device. It has become the hot point for research and application recently. At present, the study on the hydrodynamic performance of vertical-axial tidal current turbine is almost on 2-D numerical simulation, without the consideration of 3-D effect. CFD (Computational Fluid Dynamics) method and blade optimal control technique are used to improve accuracy in the prediction of tidal current turbine hydrodynamic performance. Numerical simulation of vertical-axial tidal current turbine is validated. Fixed and variable deflection angle turbine are comparatively studied to analysis the influence of 3-D effect and the character of fluid field and pressure field. The method, put the plate on the end of blade, of reduce the energy loss caused by 3-D effect is proposed. The 3-D CFD numerical model of vertical-axial tidal current turbine hydrodynamic performance in this study may provide theoretical, methodical and technical reference for the optimal design of turbine

15

Biomass allocation, compensatory growth and internal C/N balance of Lolium perenne in response to defoliation and light treatments

71%

Wang L., Wang J., Liu W., Gan Y., Wu Y.

Polish Journal of Ecology

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2016

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tom 64

|

nr 4

Light environments can have a considerable influence on how plants respond to defoliation through influencing the biomass allocation patterns and internal C/N ratio. Seedlings of Lolium perenne, a common perennial grass species, were grown for eight weeks under three different light environments (natural light, red light and shading) and two different defoliation treatments (no defoliation versus 50% aboveground biomass removal). This study was conducted to examine (1) the effects of light regimes and defoliation on biomass accumulation, biomass allocation and internal C/N ratio status in plants; (2) how the light regimes influence the pattern of compensatory growth after defoliation; and (3) the relationship between compensatory growth and the internal C/N ratio status. We found that red light altered the shoot-to-root allometry, enhanced the leaf C concentrations and induced N deficiency. By contrast, the leaf N concentrations of L. perenne were greater during shading treatment, which simultaneously enhanced shoot growth and stopped root growth. Under defoliation, red light increased shoot growth, not at the expense of root growth, which was not the same as in natural light and shading treatment. Moreover, regardless of the unclipped (no defoliation) and defoliation conditions, the L. perenne biomass partitioning between roots and shoots was significantly correlated with the leaf N concentrations and C/N ratio, indicating that allometric biomass allocation can be largely modulated by signals related to the C and N status of the plants. These results demonstrated that the leaf C and N status would be an appropriate indicator of compensatory growth after defoliation.

16

Bindarit attenuates pain and cancer-related inflammation by influencing myeloid cells in a model of bone cancer

71%

Liu S., Gao H., Gao C., Liu W., Xing D.

Archivum Immunologiae et Therapiae Experimentalis

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2018

|

tom 66

|

nr 3

17

Effects of salt stress and exogenous Ca2plus on Na plus compartmentalization, ion pump activities of tonoplast and plasma membrane in Nitraria tangutorum Bobr. leaves

71%

Liu W., Yuan X., Zhang Y., Xuan Y., Yan Y.

Acta Physiologiae Plantarum

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2014

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tom 36

|

nr 08

Nitraria tangutorum Bobr. is a typical halophyte with superior tolerance to salinity. However, little is known about its physiological adaptation mechanisms to the salt environment. In the present study, N. tangutorum seedlings were treated with different concentrations of NaCl (100, 200, 300 and 400 mmol L⁻¹) combined with five levels of Ca²⁺ (0, 5, 10, 15 and 20 mmol L⁻¹) to investigate the effects of salt stress and exogenous Ca²⁺ on Na⁺ compartmentalization and ion pump activities of tonoplast and plasma membrane (PM) in leaves. Na⁺ and Ca²⁺ treatments increased the fresh weight and dry weight of N. tangutorum seedlings. The absorption of Na⁺ in roots, stems and leaves was substantially increased with the increases of NaCl concentration, and Na⁺ was mainly accumulated in leaves. Exogenous Ca²⁺ reduced Na⁺ accumulation in roots but promoted Na⁺ accumulation in leaves. The absorption and transportation of Ca²⁺ in N. tangutorum seedlings were inhibited under NaCl treatments. Exogenous Ca²⁺ promoted Ca²⁺ accumulation in the plant. Na⁺ contents in apoplast and symplast of leaves were also significantly increased, and symplast was the main part of Na⁺ intracellular compartmentalization. The tonoplast H⁺-ATPase and H⁺-PPase activities were significantly promoted under salt stress (NaCl concentrations ≤ 300 mmol L⁻¹). PM H⁺-ATPase activities gradually increased under salt stress (NaCl concentrations ≤ 200 mmol L⁻¹) followed by decreases with NaCl concentration increasing. The tonoplast H⁺-ATPase, H⁺- PPase and PM H⁺-ATPase activities increased first with the increasing exogenous Ca²⁺ concentration, reached the maximums at 15 mmol L⁻¹ Ca²⁺, and then decreased. The tonoplast and PM Ca²⁺-ATPase activities showed increasing trends with the increases of NaCl and Ca²⁺ concentration. These results suggested that certain concentrations of exogenous Ca²⁺ effectively enhanced ion pump activities of tonoplast and PM as well as promoted the intracellular Na⁺ compartmentalization to improve the salt tolerance of N. tangutorum.

18

Optimized microwave-assisted extraction of 6-gingerol from Zingiber officinale Roscoeand evaluation of antioxidant activity in vitro

71%

Liu W., Zhou C.-L., Zhao J., Chen D., Li Q.-H.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2014

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tom 13

|

nr 2

Background. 6-Gingerol is one of the most pharmacologically active and abundant components in ginger, which has a wide array of biochemical and pharmacologic activities. In recent years, the application of microwave-assisted extraction (MAE) for obtaining bioactive compounds from plant materials has shown tremendous research interest and potential. In this study, an efficient microwave-assisted extraction (MAE) techniąue was developed to extract 6-gingerol from ginger. The extraction efficiency of MAE was also compared with conventional extraction techniques. Material and methods. Fresh gingers (Zingiber officinale Rosc.) were harvested at commercial maturity (originally from Shandong, laiwu, China). In single-factor experiments for the recovery of 6-gingerol, proper ranges of ratio of liquid to solid, ethanol proportion, microwave power, extraction time were determined. Based on the values obtained in single-factor experiments, a Box-Behnken design (BBD) was applied to determine the best combination of extraction variables on the yield of 6-gingerol. Results. The optimum extraction conditions were as follows: microwave power 528 W, ratio of liquid to solid 26 mL g1, extraction time 31 s and ethanol proportion 78%. Furthermore, more 6-gingerol and total polyphenols contents were extracted by MAE than conventional methods including Maceration (MAC), Stirring Extraction (SE), Heat reflux extraction (HRE), Ultrasound-assisted extraction (UAE), as well as the antioxidant capacity. Conclusion. Microwave-assisted extraction showed obvious advantages in terms of high extraction efficiency and antioxidant activity of extract within shortest extraction time. Scanning electron microscopy (SEM) images of ginger powder materials after different extractions were obtained to provide visual evidence of the disruption effect. To our best knowledge, this is the first report about usage of MAE of 6-gingerol extraction from ginger, which could be referenced for the extraction of other active compounds from herbal plants.

19

Research an a method of hull form design based on wave-making resistance optimization

71%

Sun J., Lv X., Liu W., Ning H., Chen X.

Polish Maritime Research

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2012

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tom 19

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nr 3

In this paper, we consider an optimization of the hull shape in order to minimize the total resistance of a ship. The total resistance is assumed to be the sum of the wave resistance computed on the basis of the thin-ship theory and the frictional resistance. Smoothness of hull lines is proved with mathematical procedure, in which differentials of the hull lines functions are analyzed. The wave-making resistance optimization, involving a genetic algorithm, uses Michell integral to calculate wave resistance. A certain hull form is generated by the method using cross section information of a modified DTMB model ship 5415 and a comparative experiment is carried out. Experimental and calculation result show that the method is of good adaptability for designing certain types of ships with excellent resistance performance

20

Molecular characterization and tissue transcription profile analyses of three novel pig genes - NDP, CLN3 and NCF1

71%

Wang G. P., He Y. Y., Hu S. F., Liu W., Liu G. Y.

Journal of Animal and Feed Sciences

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2009

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tom 18

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nr 1

Ograniczanie wyników

Theaflavin-3, 3′- digallate attenuates rheumatoid inflammation in mice through the nuclear factor-kappaB and MAPK pathways

Experiment and modeling of soil-water characteristic curve of unsaturated soil in collapsing erosion area

VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer

Effect of including different levels of palm kernel cake to dairy cow concentrate on milk production and composition

Winter food availability limits winter survival of Mongolian gerbils (Meriones unguiculatus)

Activated carbon prepared from semi-coke as an effective adsorbent for dyes

The port service ecosystem research based on the Lotka-Volterra model

A review of progress in current research on Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)

Overexpression of Rosa roxburghii L-galactono-1,4-lactone dehydrogenase in tobacco plant enhances ascorbate accumulation and abiotic stress tolerance

A PSBM model for environmental efficiency evaluation and its application

Identification and characterization of differentially expressed genes from tobacco roots after decapitation