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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Application of silymarin in human and animal medicine

100%
Radko L., Cybulski W.
Journal of Pre-Clinical and Clinical Research
|
2007
|
tom 01
|
nr 1
2

Jakość pasz dla zwierząt laboratoryjnych jako czynnik otrzymanych wyników badań

100%
Radko L., Stypula-Trebas S.
Pasze Przemysłowe
|
2018
|
tom 27
|
nr 2
3

Srodki farmaceutyczne z grupy beta-agonistow - aspekty farmakologiczne, toksykologiczno-higieniczne i legislacyjne

100%
Cybulski W., Radko L.
Życie Weterynaryjne
|
2006
|
tom 81
|
nr 11
760-763
4

Enrofloksacyna - aspekty stosowania u zwierzat i jej wplyw na srodowisko naturalne

100%
Cybulski W., Radko L.
Magazyn Weterynaryjny
|
2009
|
tom 18
|
nr 02
166-168
5

Fipronil i pyriprol - zagrozenia w terapii i dla srodowiska

100%
Cybulski W., Radko L.
Magazyn Weterynaryjny
|
2010
|
tom 19
|
nr 01
78
6

In vivo study of the oestrogenic activity of milk

100%
Radko L., Posyniak A.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 3
7

Cytotoxicity of some nitroimidazole derivatives - comparative studies on human and rat hepatoma cell lines

100%
Radko L., Minta M.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The cytototoxic potential of metronidazole, tinidazole, ronidazole, and ornidazole, using human and rat hepatoma cell lines (HepG2 and FaO) in culture was assessed. The cells were treated with drugs for 24, 48 and 72 h at 37 °C in 5% CO₂ at concentrations of 0.1 to 200 µg/mL. Following the treatment period, the cells were assayed by four independent assays: MTT reduction, neutral red uptake (NRU), total protein content (TPC), and LDH leakage. The results suggest that nitroimidazoles are of low cytotoxic potential (EC₅₀ >200µg/mL). The exception was ronidazole, which demonstrated a distinct endpoint sensitivity related to the species. EC₅₀ (µg/mL) in human cells were: in MTT assay - 196±5.5 and 122±9.3 at 24 and 48 h, respectively, and in NRU assay - 150±1.25 at 72 h. Based on minimal toxic concentrations (EC₂₀) for ronidazole, determined by all methods used in HepG2 cells, it could be concluded that their sensitivity was as follows: MTT>NRU>LDH>TPC.
8

Zatrucia zwierzat jadami owadow blonkoskrzydlych

100%
Radko L., Cybulski W.
Magazyn Weterynaryjny
|
2005
|
tom 14
|
nr 02
59-62
9

Sylimaryna przydatnym i bezpiecznym fitofarmaceutykiem w weterynarii

100%
Cybulski W., Radko L.
Herba Polonica
|
2006
|
tom 52
|
nr 3
120-121
10

Zastosowanie sylimaryny u zwierzat

100%
Radko L., Cybulski W.
Magazyn Weterynaryjny
|
2007
|
tom 16
|
nr 02
52-54
11

Optymalizacja testu wzrostu masy macicy i testu Hershbergera na chomikach z użyciem związków modelowych

81%
Radko L., Minta M., Stypula-Trebas S., Zmudzki J.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2011
|
tom 48
12

Differential toxicities of albendazole and its two main metabolites to Balb/c 3T3, HepG2, and FaO lines and rat hepatocytes

81%
Radko L., Minta M., Stypula-Trebas S.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 4
13

Badania cytotoksycznosci monenzyny i narazyny w hodowli linii ciaglej hepatocytow szczura

81%
Radko L., Cybulski W., Wessely-Szponder J., Rzeski W.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 07
834-836
The aim of the work was to determine monensin, narasin hepatotoxicity and the nature of cell death. Rat hepatocyte model cell line (FAO) was used to investigate two ionophore antibiotic cytotoxic effects estimated by MTT, NRU and KB tests approved by INVITTOX. Additionally, the apoptotic/necrotic nature of cell death was determined by propiodine iodide and HO 342 staining of the cultured hapatocytes. IC₅₀ indices for monensin and narasine estimated by using the MTT test during a 24 hour incubation period were at a level of 0,027 ± 0,001 µM and 0,037 ± 0,001 µM, respectively. However, an incubation period of 48 hrs yielded an equal value - 0,02 µM - for both ionophores. Contrary to the MTT test, NRU and KB estimations demonstrated lower IC₅₀ values for narasine than for monensin. These results correlated to in-vivo acute toxicity and LD₅₀ indices in rats (data from references). The apoptotic nature of hepatocyte death dominated in the cultures. The article also discussed the mechanisms of ionophore induced cytotoxicity.
14

Cytotoksycznosc salinomycyny i lazolocydu wobec hepatocytow modelowej linii komorkowej szczura

81%
Radko L., Cybulski W., Rzeski W.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 07
839-842
The aims of the study were determining the median cytotoxicity indicate (IC50), nature of cell death (apoptosis/ necrosis), assessment and morphology of changes observed in FAO cell line culture of hepatocytes subjected to ionophore antibiotics, salinomycin and lasalocid, incubations. INVTTOX recommended MTT, NRU and KB cytotoxicity tests were used to research mitochondrial, protein synthesis and cell proliferation. In addition cell staining in order to reveal membrane destruction that established cell death character May-Grunwald & Giemsa staining were also conducted. Cytotoxicity indices (IC50) estimated by the 24 hour MTT test were at a level 2.41 ± 0.29 mM and 7.93 ± 0.01 mM; however, after a 48 hour incubation the values lowered to 0.112739 ± 0.01 mM and 0.59 ± 0.01 mM for salinomycin and lasalocid, respectively. In contrast to the MTT data, that of NRU and KB tests were higher, indicating mitochondria as the main subcellular target for the antibiotic action. The determined IC50 values were positively related to DL50 (the data from references). Hepatocytes death were established to be of an apoptosis nature. Cell morphology was changed from IC50 depending on manner; the lower value of the indicate corresponded to more pronounced cytopathologic findings. Summarizing, monolayer cell cultures of rat hepatocytes proved to serve as a useful model for cytotoxicity studies enabling to indicate subcellular targets for ionophore antibiotics
15

Influence of fluoroquinolones on viability of Balb/c 3T3 and HepG2 cells

81%
Radko L., Minta M., Stypula-Trebas S.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 4
The cytotoxic potential of fluoroquinolones (enrofloxacin, ciprofloxacin, difloxacin, sarafloxacin, danofloxacin, norfloxacin and marbofloxacin) was investigated using mouse fibroblasts Balb/c 3T3 and human hepatoma HepG2 cell lines. The cells were exposed for 24, 48, and 72 h to drugs at eight concentrations ranged from 0.78 to 100 µg/mL. Four independent cytotoxicity assays were applied, in which various endpoints were assessed: mitochondrial activity - MTT reduction, lysosomal activity - neutral red uptake, total protein content, and cellular membrane integrity - lactate dehydrogenase release. Mean effective cytotoxic concentrations (EC₅₀) calculated at different time points from concentration-response curves ranged from 10 to 100 µg/mL. The most affected endpoint in both cell lines was mitochondrial activity. The EC₅₀₋MTT₋₇₂ₕ <10 µg/mL was found for difloxacin, marbofloxacin (fibroblasts), sarafloxacin, and norfloxacin (HepG2). The data shows that cytotoxicity of the fluoroquinolones appears after longer exposure of both cell cultures to these compounds.
16

Ocena aktywności hormonalnej prenyloflawonoidu chmielowego w kontekście stosowania odpadów browarnianych w żywieniu zwierząt

81%
Stypula-Trebas S., Radko L., Posyniak A.
Pasze Przemysłowe
|
2018
|
tom 27
|
nr 2
17

Jakość pasz dla zwierząt laboratoryjnych jako czynnik otrzymanych wyników badań

81%
Radko L., Stypula-Trebas, Posyniak A.
Pasze Przemysłowe
|
2018
|
tom 27
|
nr 4
18

Ocena aktywności hormonalnej wybranych antybiotyków wykrywanych w paszach

81%
Stypuła-Trebas S., Radko L., Posyniak A.
Pasze Przemysłowe
|
2018
|
tom 27
|
nr 2
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cytoprotective effect of silybin against lasalocid-induced toxicity in HepG2 cells

81%
Radko L., Cybulski W., Rzeski W.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 2
Lasalocid is an ionophore coccidiostatic agent frequently used in poultry. Its extensive use causes the formation of residues in edible tissues and eggs which may pose a risk to consumers. Silybin is the main compound extracted from the herb milk thistle Silybum marianum and its hepatoprotective effect has been reported in literature. The aim of the study was to compare lasalocid and silybin cytotoxic effects followed by their combined use in HepG2 cell line. A cytoprotective effect resulting from the interaction of both pharmacologically active substances was measured. In this study, an MTT test, coomassie brillant blue binding test, and LDH release test determined the effective concentration (EC₅₀) of the compounds. The isobolograms and combination index were used to assess the nature of interaction. The lowest EC₅₀-value for lasalocid was established via the MTT test. This study revealed a lack of silybin cytotoxic effect on the cells. Co-actions of the two drugs led to a significant decrease of lasalocid cytotoxicity. The isobolograms and combination index showed a remarkable antagonistic effect in the course of silybin and lasalocid interaction. The results indicate that silybin revealed a cytoprotective effect when incubated with lasalocid since its cytotoxic impact on HepG2 cells has been significantly diminished.
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Estrogenic activity of commercial milk as revealed in immature hamster uterotrophic assay – pilot study

81%
Radko L., Minta M., Stypula-Trebas S.
Roczniki Państwowego Zakładu Higieny
|
2017
|
tom 68
|
nr 3
Background. The risk for public health posed by endocrine disruptors present in food is relatively new issue. Our current understanding of human exposure is mainly based on the residue analysis of selected compounds. With such approach potential, effects of mixtures, including so-far unidentified compounds are not taken into consideration. Therefore, the knowledge of overall hormonal activity in food samples is of big importance. Objective. Milk and dairy products are a rich source of estrogens but very rarely undergo testing for estrogenic activity. For this reason the rodent uterotrophic bioassay is one of the most useful tool. This preliminary study was conducted in immature hamsters to assess commercially available milk. The endpoint measured was uterine weight increase. Material and methods. Fifteen-day old females received ad libitum throughout 7 days commercially available milk i.e. raw goat’s, raw cow’s, processed 3.2% UHT, and for comparison soy milk. The animals of negative control group received water but positive control group got 17β - estradiol (E2) at the concentration of 100 ng/ml. Results. All samples of milk showed estrogenic activity as follow: goat’s >cow’s >soy >processed milk. Significant increase of uteri weights were recorded in goat’s (p<0.001) and cow’s milk (p<0.01). However, the activity was approximately 5-fold lower than induced by 17β-estradiol. The ratio uterine weight/body weight (%) in negative control was 0.096%, in milk experimental groups ranged from 0.112% to 0.153% and in positive control this value was 0.493%. Conclusion. The results suggest that commercially available milk has a weak uterotrophic activity. Further in vivo and in vitro studies are warranted to gain more insight into the estrogenic risk from milk and other dairy products.
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