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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The regulation of chloride balance in calves during the first week of life

100%
Dratwa-Chalupnik A., Herosimczyk A.
Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica
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2014
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tom 31
Badania przeprowadzono na 10 klinicznie zdrowych cieliczkach rasy polsko-fryzyjskiej odmiany czarno-białej, w ciągu pierwszych 7 dnia życia postnatalnego. Celem prezentowanych badań była analiza nerkowej regulacji bilansu chlorków podczas pierwszego tygodnia życia cieląt. Stężenie chlorków w osoczu krwi cieląt w pierwszym tygodniu życia wynosiło średnio 98,86 mmol · l–1 i mieściło się w granicach norm fizjologicznych. Do piątego dnia życia koncentracja tego elektrolitu była stabilna, a następnie w siódmym dniu życia nieznacznie się obniżyła. Przy ujednoliconym żywieniu (siarą i mlekiem matek) obserwowane zmiany w kolejnych dniach pierwszego tygodnia życia związane były głównie ze zmianami czynności nerek (przesączania w kłębkach i resorpcji kanalikowej). W pierwszym tygodniu życia cieląt obserwowano istotne zmiany wielkości ładunku przesączonego, resorpcji kanalikowej oraz wydalania chlorków z moczem. Analiza zmian wielkości ładunku przesączonego chlorków wskazuje, że zmiana stężenia chlorków w osoczu krwi nie miała wpływu na ten wskaźnik. Stwierdzono, że decydującym mechanizmem regulującym wydalanie chlorków z moczem są zmiany wielkości resorpcji tego elektrolitu w kanalikach nerkowych. Uzyskane wyniki wskazują, że nerki cieląt noworodków są dostatecznie przygotowane do regulacji stężenia chlorków w osoczu krwi.
2
Content available remote

Defining the blood plasma protein repertoire of seven day old dairy calves - a preliminary study

81%
Skrzypczak W. F., Ozgo M., Lepczynski A., Herosimczyk A.
Journal of Physiology and Pharmacology
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2011
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tom 62
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nr 3
During the early postnatal period in calves various adaptational changes occur. These functional, morphological and also metabolic alteration are reflected by blood plasma protein changes as they are secreted and shed from many cells and tissues. Blood plasma protein pattern of an adult cattle differs in some respect when compared with neonatal calves. There exist a very few data concerning 2-D maps of neonatal calves blood plasma. The above prompted us to establish protein pattern of this biological fluid characteristic of healthy, 7 day old, Polish Black-and-White (Polish Friesian) breed calves. Blood plasma proteins of the isoelectric point ranging from 4.0 to 7.0 were analyzed by the aid of high resolution two-dimensional electrophoresis (2-DE). Subsequently, 79 excised protein spots corresponding to 23 different gene products were identified using matrix-assisted laser desorption/ionisation mass spectrometer (MALDI-TOF MS). Protein map obtained in the present study may be useful in assessing the changes in the calves blood plasma protein profiles occurring in response to different physiological and/or pathophysiological factors.
3

Proteomika a fizjologia i patofizjologia nerek

81%
Ozgo M., Skrzypczak W. F., Herosimczyk A., Mazur A.
Medycyna Weterynaryjna
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2007
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tom 63
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nr 10
1146-1150
Proteomics is a new, dynamically developing branch of science. It uses specific research tools which enable analysis of a whole protein expression in definite time and a concrete biological system. The purpose of current applications of renal and urinary proteomics are to better understand renal physiology, to explore the molecular mechanisms of the occurrence and progression of diseases, and also to identify protein biomarkers. This review is intended to discus the present status of the contribution of proteomic analysis to nephrology.
4

Calves with diarrhea and water-electrolyte balance

81%
Dratwa-Chalupnik A., Herosimczyk A., Lepczynski A., Skrzypczak W. F.
Medycyna Weterynaryjna
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2012
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tom 68
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nr 01
Excessive water and electrolyte loss through the gastrointestinal (GI) tract during diarrhea is the most common cause of dehydration, which in acute cases can lead to death. Causes of diarrhea include environmental, nutritional and infective factors. During the course of diarrhea, electrolytes responsible for decreased water absorption accumulate in the GI tract. Together with an increasing degree of dehydration in calves the following are observed: hyponatremia, hyperkalemia and hypochloremia. Water and electrolyte imbalance implicates haematological and metabolic changes: decrease in glucose concentration, increase in urea and creatinine concentration in blood, loss of carbohydrates and accumulation of organic acid, which conduce to the appearance of metabolic acidosis. The kidneys are mainly responsible for maintaining a water and electrolyte balance. Water and electrolyte imbalance is relatively easily achieved in neonatal calves during diarrhea as a result of a limited renal function and lower than in adults efficiency of renal hormonal regulation.
5

Effect of age food intake on the selected blood plasma/serum proteins in calves during the early postnatal period

81%
Herosimczyk A., Lepczynski A., Ozgo M., Skrzypczak W. F.
Medycyna Weterynaryjna
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2012
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tom 68
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nr 05
The most intense adaptive changes to the extrauterine environment occur during the first week of calves’ lives. These changes involve many vital systems, including the gastrointestinal tract. Bovine colostrum contains various essential nutrients and supplies newborn calves with energy and also bioactive factors. Intestinal absorption of these molecules and its further passage into the bloodstream is only possible within the first 24 hours of life. Thus, the exact time of the first colostrum intake is crucial for the newborn calf, as it initiates a number of physiological processes, which results in, e.g., increased synthesis of endogenous proteins. The placenta of bovine species prevents the effective transfer of maternal plasma proteins to the conceptus, thus newborn calves are considered as a suitable model for the study of blood plasma protein profile changes with age and in response to the food intake. This review is intended to discuss the present state of knowledge within this subject.
6
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Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves

81%
Lepczynski A., Herosimczyk A., Ozgo M., Skrzypczak W. F.
Polish Journal of Veterinary Sciences
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2015
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tom 18
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nr 1
The study was undertaken to determine the effect of feeding milk or milk-replacer on the blood plasma proteome and lipid profile in calves during the second week of life. Feeding milk-replacer significantly decreased the expression of plasma apoA-I. Age of calves affected apoA-I expression, which was higher on the 8th than on the 11th and 14th day of life. A significant effect of interaction between diet and age was also observed. The expression of apoA-IV, was significantly affected by diet and was lower in calves fed milk replacer. Expression of this protein was significantly lower at the 8th day of life and was up-regulated in the calves fed milk-replacer at the second week of life. Calves fed milk-replacer had greater expression of haptoglobin, which differed significantly between days of blood sampling, being higher on the 8th than on the11th and 14th day. The interactive effect of diet and age affected haptoglobin expression, which was successively down-regulated in calves fed milk replacer. Diet had a significant effect on the plasma lipid profile. Animals fed milk had a greater concentration of TC, HDLC and LDLC. The composition of milk-replacer, especially fat source, is probably the main factor that affects expression of proteins involved in cholesterol metabolism and level of components of lipid profile in calves fed formula. We claim that the initially increased level of haptoglobin, followed by its decrease during the second week of life in calves fed milk-replacer may indicate the presence of short-term stress induced by changes in the feeding system.
7

Changes of copper concentration in blood plasma of kids in neonatal period. The influence of sex and litter size

81%
Ozgo M., Skrzypczak W. F., Herosimczyk A., Kowalska J.
Acta Scientiarum Polonorum. Medicina Veterinaria
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2007
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tom 06
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nr 1
8

Application of proteomic tools to identify changes in the protein composition of physiologically relevant fish tissues

71%
Colussi A., Ozgo M., Herosimczyk A., Michalek K., Skrzypczak W. F.
Medycyna Weterynaryjna
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2013
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tom 69
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nr 05
Over the past ten years, proteomic tools have been extensively used in various studies aimed at a better understanding of fish biology and aquaculture. A variety of post-genomic techniques have been developed and adapted for protein profiling of complex biological samples, such as tissues. This review will discuss the applications of expression proteomics in fish health monitoring with regard to nutritional and environmental changes, as well as bacterial or viral infection, in selected tissues relevant to fish physiology, i.e. the kidney, brain, blood plasma, and liver.
9
Content available remote

The current proteomic landscape of the porcine liver

71%
Ozgo M., Lepczynski A., Robak P., Herosimczyk A., Marynowska M.
Journal of Physiology and Pharmacology
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2019
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tom 70
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nr 3
10

Plasma proteomic profiling of African catfish (Clarias gariepinus)

61%
Herosimczyk A., Ozgo M., Lepczynski A., Ciechanowicz A., Colussi A., Seniuta P., Skrzypczak W. F.
Medycyna Weterynaryjna
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2013
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tom 69
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nr 10
This study was aimed at profiling plasma proteins of healthy 8-month-old African catfish. Plasma proteins within the isoelectric point ranging from 3.0 to 10.0 were separated by high resolution two-dimensional electrophoresis (2-DE). In total, 8 different gene products were identified with a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer. These included carrier proteins (fatty acid-binding protein 1, metallothionein, mitoferrin-2), a structural protein (vimentin), proteins involved in the regulation of transcription (thyroid hormone receptor alpha A, endothelial differentiation-related factor 1 homolog), and others (B-cell lymphoma 6 protein homolog, FGFR1 oncogene partner 2 homolog). This is the first study attempting to map the plasma proteome of Clarias gariepinus. Owing to the lack of information concerning catfish protein sequences in the relevant databases, protein spots were identified by matching peptide data to interspecies homology. The results of this preliminary study may encourage other authors to undertake further research on the plasma proteome of the African catfish.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Blood plasma protein and lipid profile changes in calves during the first week of life

61%
Herosimczyk A., Lepczynski A., Ozgo M., Dratwa-Chalupnik A., Michalek K., Skrzypczak W. F.
Polish Journal of Veterinary Sciences
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2013
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tom 16
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nr 3
The present study was undertaken to determine blood plasma protein and lipid profile changes in healthy Polish Holstein-Fresian calves of Black-and-White variety. Blood was drawn immediately after birth, before first colostrum intake and at the 3rd, 6th, 12th, 24th, 36th, 48th and 72nd hour of life. Subsequent four blood samples were collected at 24 hour intervals until the 7th day of life. Plasma proteins within the isoelectric point ranging from 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. Among the 74 protein spots detected and analyzed, 16 were significantly altered during the first week of life. Differentially expressed spots were excised from the gels and subjected to peptide mass fingerprinting using MALDI-TOF MS. In total, 12 spots were successfully identified, which correspond to three proteins, namely: apolipoprotein A-I, apolipoprotein A-IV and fibrinogen gamma-B chain. A gradual increase in plasma triglyceride, total cholesterol, HDL and LDL cholesterol values was shown during the first seven days of calves life. The lowest concentration of these indicators were observed at birth and was followed by a rapid increase during the first week of postnatal life. These changes appear to be related to the transition in energy sources, from a maternal nutrient supply comprising mainly carbohydrates and amino acids to a diet which was rich in fat – colostrum and milk. This was reflected by the intense up-regulation of plasma proteins related with lipid transport and lipoprotein metabolism during the first week of life.
12
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Dietary supplementation with dried chicory root triggers changes in the blood serum proteins engaged in the clotting process and the innate immune response in growing pigs

61%
Lepczynski A., Herosimczyk A., Ozgo M., Skomial J., Taciak M., Barszcz M., Berezecka N.
Journal of Physiology and Pharmacology
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2015
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tom 66
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nr 1
13

Renal regulation of potassium homeostasis in calves in the first week of life including the role of atrial natriuretic peptide

61%
Dratwa-Chalupnik A., Ozgo M., Herosimczyk A., Lepczynski A., Michalek K., Skrzypczak W.
Folia Biologica
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2011
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tom 59
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nr 3-4
14

Comparative study of selected blood biochemical components in milk or milk-replacer fed calves during the second week of life

61%
Lepczynski A., Herosimczyk A., Dratwa-Chalupnik A., Ozgo M., Michalek K., Malinowski E., Skrzypczak W. F.
Folia Biologica
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2011
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tom 59
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nr 3-4
15
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Plasma proteome analysis: 2D gels and chips

61%
Herosimczyk A., Dejeans N., Sayd T., Ozgo M., Skrzypczak W. F., Mazur A.
Journal of Physiology and Pharmacology. Supplement
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2006
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tom 57
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nr 7
81-93
The knowledge of concentration, modification and interaction of proteins is fundamental in determining the phenotype of living organisms. Plasma, the primary clinical specimen, contains numerous and diverse proteins. The functions of these proteins are as manifold as the diversity of the protein themselves. Many of them have been largely used for many years as biomarkers of diseases and indicators of the physiological functions. The study of plasma proteome promises to be a significant advance in various areas of biological and clinical research. Two-dimensional polyacrylamide gel electrophoresis is considered as a primary tool in separating thousand of plasma proteins. This approach enables comparing normal and diseased samples revealing differently expressed proteins. Other proteomic techniques suitable for plasma analysis such as protein microarrays are now either established or are still being improved. This article briefly reviews the application of two-dimensional electrophoresis and the current status of technical aspects for plasma proteome.
16

Modification of ileal proteome in growing pigs by dietary supplementation with inulin or dried chicory root

61%
Lepczynski A., Herosimczyk A., Ozgo M., Barszcz M., Taciak M., Skomial J.
Journal of Animal and Feed Sciences
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2019
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tom 28
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nr 2
Recently, numerous plant-based preparations have been used for health promotion or disease prevention in animals. Chicory is one of the plants that contain various nutraceutics, mainly inulin type fructans (ITFs) showing prebiotic character. In pigs, a significant proportion of ITFs is fermented in the distal part of the small intestine to lactate and short-chain fatty acids (SCFAs). Previous studies have shown that SCFAs are involved in structural rearrangement of the intestinal epithelial mucosa and stimulate intestinal barrier assembly. These changes should be accompanied by a modification of enterocyte protein composition and abundance. Thus, we hypothesized that ITFs, due to their direct or indirect effect, can modify ileal proteome. The experiment was performed on 24 castrated male pigs (PIC × Penarlan P76) assigned to 3 groups (n = 8) fed cereal-based diets: control or experimental: supplemented with 4% of dried chicory root or with 2% of inulin. Mucosa proteins were separated using two-dimensional electrophoresis, followed by the identification of statistically valid proteins with the aid of Matrix Associated Laser Desorption Ionization – Time of Flight mass spectrometry (MALDI-TOF MS). Experimental diets significantly altered expression of proteins involved in: glycolysis/gluconeogenesis, biosynthesis of amino acids, cytoskeleton rearrangement, protein synthesis and processing, cell proliferation and differentiation, and iron absorption. Changes in the expression of proteins associated with energetic metabolism, cell proliferation and cytoskeleton rearrangement may suggest an impact of dried chicory root on the functional maturation of the ileal mucosa. Additionally, changes in transferrin abundance suggest the significance of chicory root and inulin supplementation for intestinal iron absorption.
17

Kidney proteomic profiling of African catfish

61%
Robak P., Ozgo M., Herosimczyk A., Lepczynski A., Pyc A., Skrzypczak W. F.
Medycyna Weterynaryjna
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2015
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tom 71
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nr 05
The purpose of this study was to create a two-dimensional (2-D) profile of kidney proteins characteristic of African catfish (Clarias gariepinus). We prepared proteins from kidney tissues of healthy 8-month-old African catfish. Samples were subjected to separation using high resolution two-dimensional electrophoresis (2-DE) and stained with colloidal Coomassie Brilliant Blue G-250. Among the 96 protein spots detected on each 2-DE gel, 9 different proteins were identified by means of a matrix-assisted laser desorption/ionization (MALDI) time-of- -flight (TOF) mass spectrometer. These proteins belong to the following groups: structural (actin and tubulin), involved in fat transport (fatty-acid binding protein), involved in cell cycle regulation (ubiquitin-protein ligase), responsible for cell metabolism (ATP synthase, hypoxanthine-guanine phosphoribosyltransferase, enolase) and molecular chaperone (heat shock protein 70 kDa). The presented in the current study two-dimensional map of kidney proteins may provide a basis for further refinements in the proteomic analysis of fish, including African catfish. The final result of comprehensive proteomic analysis may be a better understanding of kidney physiology and pathology, as well as identification of health and disease-related biomarkers.
18

Age-related changes of selected blood biochemical indicators in dairy calves during their first week of life

61%
Herosimczyk A., Lepczynski A., Dratwa-Chalupnik A., Kurpinska A., Klonowska A., Skrzypczak W. F.
Folia Biologica
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2011
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tom 59
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nr 1-2
19
Content available remote

Urinary excretion of low molecular weight proteins in goats during the neonatal period

61%
Ozgo M., Skrzypczak W., Drzezdzon D., Lepczynski A., Dratwa-Chalupnik A., Michalek K., Herosimczyk A.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
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nr 3
119-125
20

Inulin-type fructans trigger changes in iron concentration and activity of bone metabolism biomarkers in blood plasma of growing pigs

61%
Lepczynski A., Herosimczyk A., Barszcz M., Ozgo M., Taciak M., Skomial J.
Journal of Animal and Feed Sciences
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2016
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tom 25
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nr 4
The aim of the study was to estimate the influence of dietary supplementation with dried chicory root and native chicory inulin on biochemical profile of blood plasma. In total 24 male piglets were divided into 3 groups (n = 8) and fed unsupplemented cereal-based diet (C diet), diet with 2% of native chicory inulin (T1 diet) or with 4% of dried chicory root (T2 diet). After 40 days of feeding pigs were sacrificed and blood plasma samples were taken for biochemical analysis. Plasma albumin concentration, as well as alkaline phosphatase and alanine aminotransferase activity, were significantly higher in the group of animals fed T2 diet. Plasma γ-glutamyltransferase activity was significantly lower in the pigs fed T2 diet when compared to animals fed C and T1 diets. Significant increase in plasma iron concentration in animals fed T2 diet and slightly increased calcium level in both treatment groups were observed. It can be concluded that dietary fructans and dried chicory root do not affect liver functioning. Feeding growing pigs diet supplemented with dried chicory root significantly improves plasma iron concentration and may increase blood plasma calcium concentration what, in turn, may enhance bone mineralization and growth in young animals.
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