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1

Encapsulation of nodal segments of Cassia angustifolia Vahl. for short-term storage and germplasm exchange

100%
Parveen S., Shahzad A.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 03
The present study described the encapsulation of nodal segments of Cassia angustifolia Vahl. excised from 1-month-old in vitro raised cultures for short-term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were obtained through a combination of 3 % sodium alginate and 100 mM calcium chloride. The maximum conversion response (94 %) of encapsulated beads was obtained in Murashige and Skoog’s medium (MS medium) supplemented with 2.5 μM benzyladenine (BA) and 0.4 μM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated and nonencapsulated nodal segments were also stored at 4°C for different time periods (0, 1, 2, 4, 6 and 8 weeks). The regenerated microshoots were best rooted in optimized rooting medium that comprised half-strength MS + 1.0 μM indole-3-butyric acid (IBA) + 5.0 μM phloroglucinol (PG) for the production of complete plantlets. The regenerated plantlets were successfully hardened and acclimatized in natural conditions with 70 % survival rate.
2

A micropropagation protocol for Cassia angustifolia Vahl. from root explants

100%
Parveen S., Shahzad A.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 3
The aim of this study was to develop a new micropropagation system for Cassia angustifolia Vahl., an important medicinal legume using root explant as starting material. Root explants taken from 30-day-old aseptic seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators: 6-benzyladenine (BA), kinetin (Kn), and thidiazuron (TDZ). Organogenic nodular calli obtained on MS + TDZ (1.0 µM) were transferred to shoot regeneration medium supplemented with different cytokinins (BA, Kn or TDZ) either alone or in combination with auxin:indole-3-acetic acid or a-naphthalene acetic acid. Maximum shoot regeneration frequency (90%) was obtained on MS + BA (2.5 µM) + NAA (0.6 µM) wherein a maximum of 42.76 ± 1.47 shoot buds per explant were induced with a maximum conversion rate of 35.63 ± 0.75 shoots per explant and average shoot length of 5.43 ± 0.20 cm. Elongated microshoots were successfully rooted under ex vitro conditions by pulse treatment in 200 µM of indole-3-butyric acid for half an hour. Microshoots were rooted, acclimatized and hardened off simultaneously in sterilized soilrite inside the growth room and then established in pots containing sterilized soil and manure (1:1) and grown under greenhouse condition with 90% survival rate. The histological sections at different developmental stages of shoot buds revealed the organization of nodular meristematic zone leading to the orientation and differentiation of shoot buds in large number and thereafter conversion into healthy shoots.
3
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Fossilized pollination droplet in a new seed genus from the Middle Triassic of Nidpur, India

100%
Bhowmik N., Parveen S.
Acta Palaeontologica Polonica
|
2014
|
tom 59
|
nr 2
The present article reports a fossilized pollination droplet at the micropylar orifice in a compressed seed Gopadispermum papillatus gen. et sp. nov. from the Middle Triassic beds of Nidpur, Madhya Pradesh, India. The shapeless droplet forming a convexity above the micropylar orifice is comprised of a resinous crystalline substance. Entrapped within the droplet are a few saccate pollen grains. The seeds are small, oblong to widely elliptical in shape, about 3 mm long and generally 2 mm broad. The micropylar end shows a short straight beak-like micropyle often extended beyond a persistently adhering wrinkled tissue lying outside the seed coat. The seed is composed of four membranes excluding the adherent tissue. They are the outer and inner cuticles of integument, the nucellar cuticle distally modified to form a dark collar-like pollen chamber and the innermost megaspore membrane. Cuticles of the tissue adhering to seed coat are different from seed coat cuticles. The pollen grains inside the pollen chamber are frequently clumped together forming a pollen mass. Individual pollen grains appear spheroidal to ellipsoidal in shape and are saccate. This is the first report of the preservation of a pollination droplet in a compressed seed specimen from the Nidpur Triassic beds. Preservation of the droplet can be attributed to its supposed resinous constitution and the entrapped organic contents (pollen grains). Occurrence of clumped pollen grains inside the pollen chamber also indicated possibility of fluid feeding, pollinivory and insect pollination in the seeds.
4

Nickel toxicology with reference to male molecular reproductive physiology

81%
Rizvi A., Parveen S., Khan S., Naseem I.
Reproductive Biology
|
2020
|
tom 20
|
nr 1
5

Combined deficiency of nitrogen and iron increases senescence induced remobilization of plant immobile iron in wheat

81%
Parveen S., Ranjan R. K., Anand A., Singh B.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 12
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