Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 18

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

O odpornosci bez przeciwcial...

100%
Cytrynska M.
Postępy Biologii Komórki
|
2009
|
tom 36
|
nr 2
309-324
2

Protein kinase A activity in haemocytes of Galleria mellonella larvae upon immune challenge conditions

51%
Cytrynska M., Zdybcka-Barabas A., Jakubowicz T.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
3

Three Pseudomonas aeruginosa strains with different protease profiles

51%
Andrejko M., Zdybicka-Barabas A., Janczarek M., Cytrynska M.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 1
The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activity specific assays, it was concluded that the reference strain produced three proteolytic enzymes in the LB medium: protease IV, elastase B and elastase A, while alkaline protease was only produced in the M9 medium. The clinical isolates of P. aeruginosa produced elastase B and alkaline protease when grown in the LB medium and the minimal M9 medium, respectively. PCR analysis confirmed the presence of both the lasB gene encoding elastase B and aprA coding for alkaline protease in the genomes of the three P. aeruginosa strains analyzed. The expression of these genes coding for two important P. aeruginosa virulence factors was dependent on the growth conditions in all the strains studied. The contribution of the extracellular proteinases to the virulence of P. aeruginosa strains used in this study was investigated using an insect model, the greater wax moth Galleria mellonella.
4

Identification of antimicrobial peptides of greater wax moth Galleria mellonella

51%
Cytrynska M., Zdybicka-Barabas A., Jakubowicz T.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
5

Studies on lysozyme from different invertebrate species

51%
Fiolka M. J., Cytrynska M., Jablonski P. G., Jakubowicz T.
Polish Journal of Veterinary Sciences
|
2001
|
tom 04
|
nr 3
6

The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria

51%
Cytrynska M., Zdybicka-Barabas A., Jakubowicz T.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 1
The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live Gram-positive bacteria Micrococcus lysodeikticus and Gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than Gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.
7

The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris

51%
Frajnt M., Cytrynska M., Jakubowicz T.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 4
Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of cAMP such as 8-chloro-cAMP and 8-bromo-cAMP were as potent as cAMP in PKA activation while N6, 2'- O-dibutyryl-cAMP did not stimulate the enzyme activity. It was shown that protamine sulfate was almost equally phosphorylated in the presence of 1-2 X 10-6 M cAMP or cGMP while other substrates such as Kemptide, ribosomal protein S6 were phosphorylated to a lower extent in the presence of cGMP. It was demon­strated that pyruvate kinase is a substrate of PKA which co-purified with the P. pastoris enzyme. Moreover, pyruvate kinase was phosphorylated by PKA in the presence of cAMP and cGMP to comparable levels.
8

The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation

51%
Frajnt M., Cytrynska M., Jakubowicz T.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tol­erance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associ­ated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented re­sults it can be concluded that a protein kinase A signalling pathway(s) might be in­volved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
9

Phosphorylation of yeast ribosomal proteins by CKI and CKII in the presence of heparin

51%
Wojda I., Cytrynska M., Frajnt M., Jakubowicz T.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 1
We have found that heparin has a different effect on Trichosporon cutaneum ribosomal protein phosphorylation by CKI and by CKII. In the presence of heparin, modification of 13 kDa, 19 kDa and 38 kDa proteins catalyzed by CKII was inhibited, while in the case of CKI, in addition to protein of 15 kDa, phosphorylation of 20 kDa and 35 kDa proteins was detected. It was also found that, in the presence of heparin, phosphorylation of P proteins (13 kDa and 38 kDa) by ribosome-bound protein kinases was inhibited. Moreover at the same conditions modification of 40 kDa protein was observed in all four yeast species tested.
10

Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum

51%
Wojda I., Cytrynska M., Frajnt M., Jakubowicz T.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, un­like in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modifi­cation of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the pro­tein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was estab­lished at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly in­creased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis re­vealed the presence of CKI recognition motifs in the N-terminal fragment of the 40 kDa protein.
11

Phosphorylation of yeast ribosomal proteins by CKI and CHII in the presence of heparin

51%
Wojda I., Cytrynska M., Frajnt M., Jakubowicz T.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 3
12

Phosphorylation of A-proteins by protein kinases bound to yeast ribosomes

51%
Cytrynska M., Jakubowicz T., Gasior E.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 2
13

Phosphorylation of acidic ribosomal proteins by ribosome-associated protein kinases of Saccharomyces cerevisiae and Schizosaccharomyces pombe

51%
Jakubowicz T., Cytrynska M., Kowalczyk W., Gasior E.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 4
Two proteins of 13 kDa and 38 kDa, the components of 60S ribosomal subunits, were identified as phosphorylation substrates for protein kinases tightly associated with S. cerevisiae and Schizosaccharomyces pombe ribosomes. An enzyme with properties of multifunctional casein kinase II was detected in ribosome preparations from both yeast species. In S. cerevisiae another protein kinase with high substrate specificity toward those proteins was also identified. By using isoelectric focusing, the protein band of 13 kDa from S. cerevisiae and S. pombe was resolved respectively into three and four major forms of different charge. The same protein forms were phospho- rylated in the in vivo 32P-labelling experiments.
14

The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii

45%
Chmiel E., Palusinska-Szysz M., Zdybicka-Barabas A., Cytrynska M., Mak P.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 1
 Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.
15

Pseudomonas aeruginosa alkaline protease exhibits a high renaturation capability

39%
Andrejko M., Sieminska-Kuczer A., Janczarek M., Janik E., Bednarczyk M., Gagos M., Cytrynska M.
Acta Biochimica Polonica
|
2019
|
tom 66
|
nr 1
16

Alfa-1,3-glukan, składnik ściany komórkowej grzybów - nowy wzorzec molekularny patogenów?

33%
Staczek S., Wojda I., Wiater A., Pleszczynska M., Grygorczuk K., Koziej M., Brzana W., Zdybicka-Barabas A., Cytrynska M.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
17

Alpha-1,3-glucan, a fungal cell wall component, elicits humoral immune response in a model organism Galleria mellonella

33%
Staczek S., Wojda I., Wiater A., Pleszczynska M., Grygorczuk K., Koziej M., Zdybicka-Barabas A., Cytrynska M.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 1
18

Mechanizm przeciwgrzybowego działania defensyny Galleria mellonella

27%
Grygorczuk K., Staczek S., Sowa-Jasilek A., Zdybicka-Barabas A., Koziej M., Mak P., Derylo K., Tchorzewski M., Wydrych J., Skrzypiec K., Cytrynska M.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.