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Biochar amendment may improve crop growth through its nutrients and indirect fertility. However, this improvement varies in a wide range of biochars, crops, and soils. Our objectives were to determine the response of crop growth to biochar amendment and to assess the N use efficiency relative to the biochar and the soil types. In this pot experiment, we investi-gated five typical agricultural soils in China amended with two biochars. Four treatments were designed: the soil itself as a control, the soil amended with 1% biochar, the soil with fertilizer NPK, and the soil with added biochar and fertilizer. Biochar amendment increased the maize biomass and the N use efficiency in the red soil (p<0.05) but not in the other four soils (p>0.05). In the red soil, the biomass under biochar+NPK was 2.67-3.49 times higher than that of only NPK, and 1.48-1.62 times higher than that of only biochar amendment, 21-36 and 35-42% of which were contributed from biochar fertility and indirect fertility, respectively. This study indicates that biochar amendment is very plausible for the red soil but has a minor or even negative effect on the other four soils in China.
Background: Osthole is a natural product that has multiple bioactive functions and has been reported to exert potent immunosuppressive effects. However, the therapeutic effect of osthole on arthritis has not been explored. In the present study, a collagen-induced arthritis rat model, IL-1β-stimulated SW982 cells, and RA-like fibroblast-like synoviocytes (FLS) were employed to investigate the effect and possible mechanism of osthole on arthritis in vivo and in vitro. Results: 20 and 40 mg/kg osthole significantly alleviated collagen-induced arthritic symptoms based on histopathology and clinical arthritis scores, and improved erosion using HE staining. 20 and 40 mg/kg osthole decreased the level of IL-1β, TNF-α and IL-6 in rats and ameliorated oxidative stress in serum evaluated using ELISA kits. In addition, treatment with 50 and 100 μM osthole for 48 h inhibited 10 ng/ml IL-1β-stimulated proliferation and migration of SW982, and significantly inhibited the expression of matrix metalloproteinases, such as MMP-1, MMP-3 and MMP-13, as detected by western blot. 50 and 100 μM osthole also blocked the generation of IL-6 and TNF-α in IL-1β-stimulated SW982 cells. The NF-κB and MAPK pathways were also inhibited by osthole in IL-1β-treated SW982 cells. Conclusion: These results collectively demonstrated that osthole improves collagen-induced arthritis in a rat model and IL-1β-treated SW982 cells through inhibiting inflammation and cellular stress in vivo and in vitro, and osthole might be a promising therapeutic agent for RA.
For this study we applied a biological restoration technology combined with introducing filterfeeding bivalves (Hyriopsis cumingii), stocking planktivorous fish (silver carp), and replanting submerged macrophytes (Ceratophyllum demersum L.) in order to treat eutrophic water. The effects of this combined biological restoration measure on remedying eutrophic water were investigated, and the main mechanisms of this combined measure for nutrient transportation and transformation were revealed. The results showed this combined biological restoration measure effectively reduced nutrient nitrogen (N) and phosphorus (P) loads in the water. The assimilation and absorption of submerged macrophytes and the nutrient transformation from water to the sediments were the main mechanisms for water N and P removal. In conclusion, the combination of different biological restoration measures may be a potential choice for water managers in eutrophic water restoration.
In order to understand how the nutrient elements were taken up during the cell growth as well as the production of metabolites, it was quite necessary to identify the dynamic change of metabolites and nutrients in suspension cells of Panax quinquefolium in bioreactor. In this study, dynamic accumulation of biomass and ginsenosides Re, Rb1 and polysaccharide as well as major nutrients consumption in cell suspension culture of P. quinquefolium in a 5-L stirred tank bioreactor were investigated. The dry cell weight and the contents of ginsenosides Re, Rb1 and polysaccharide reached the maximum peak simultaneously on about 21 days and the results showed that cell growth and metabolites synthesis related to nutrients consumption. For this reason, we supposed that the contents of metabolites can be increased through added nutrient at the right moment. These results provided theory reference for two-stage or continuous perfusion culture in suspension cells of P. quinquefolium in bioreactor.
Biomass growth, ginsenoside and polysaccharide production in different ginseng tissue cultures, including callus culture, adventitious root culture and hairy root culture, were studied, and the active component contents were compared with that of native ginseng roots. The adventitious root culture was confirmed to be a very nice system, which grew fast and contained a rather high content of ginsenosides. Then, the culture conditions of adventitious root culture were optimized. The results showed that salt strength, various sucrose concentrations, ammonia/nitrate ratios and phosphate concentrations had significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in ginseng. The best culture conditions for ginsenoside production seemed to be 0.75 salt strength Murashige and Skoog medium, 4% sucrose, 9 mM ammonia to 36 mM nitrate, and 1.25 mM phosphate, while the optimization for polysaccharide accumulation seemed to be 0.75 salt strength, 6% sucrose, 9 mM ammonia to 36 mM nitrate and 3.75 mM phosphate source. Appropriate conditions allowed for a maximum ginsenoside yield of up to 132.90 mg/L and polysaccharide yield of 407.63 mg/L to be obtained after 4 weeks of culture.
Lactoalbumin hydrolysate (LH) at 100 mg L⁻¹ with methyl jasmonate (MJ) at 2 mg L⁻¹ synergistically stimulated ginsenoside accumulation in Panax quinquefolium cells compared with 100 mg L⁻¹ LH. Combination elicitors led to higher ginsenoside productivity (45.93 mg L⁻¹) than single treatment of 100 mg L⁻¹ LH (31.37 mg L⁻¹). This present result will be helpful in providing a tool for enhancing the productivity of ginsenoside by Panax quinquefolium cell cultures on a commercial scale.
The fate of ammonium (NH₄+) was investigated using the 15N tracer stable isotope technique in integrated vertical-flow constructed wetlands (IVCWs). Two types of IVCW systems were built: unplanted IVCWs (control) and planted IVCWs. The results showed that a high NH₄+ removal efficiency (95.4%) in the planted IVCWs. Plants not only removed NH₄+ by direct uptake (13.6±0.7%) but also improved the NH₄+removal capacity of IVCWs, compared with the control. Besides plant uptake, microbial conversion was the dominant mechanism of NH₄ + removal in IVCWs, and a large proportion (75.2-85.6%) of added NH₄+ may be permanently removed via anammox and nitrification-denitrification processes in IVCWs.
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