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Kcnb1 plays a role in the development and function of the ear in zebrafish

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Jedrychowska J., Gasanov E., Kuznicki J., Korzh V.
Acta Neurobiologiae Experimentalis
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2019
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tom 79
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nr Suppl.1
INTRODUCTION: Voltage-gated potassium channels (Kv) are selective transmembrane proteins that allow transport of K+ across the plasma membrane, where they play an important role in establishing the resting potential. They are involved in a variety of biological processes crucial for proper functioning of the cells. In this study we are focused on zebrafish Kcnb1 (Kv2.1), a member of the electrically active Kv2 subfamily of voltage-gated potassium channels. Previously, it has been shown that this protein is expressed in mammalian, Xenopus laevis, and zebrafish inner ear. Based on this, Kv2.1 could be important for development of this organ, where it may be required for proper hearing and spatial orientation. These functions include activity of the mechanosensory cells, which develop a long kinocilium used to export Ca2+ required to form and tether the “hearing stones” – the otoliths (in fish) or otoconia (in mammals). AIM(S): The aim of this study is to investigate the role of Kcnb1 during ear development using zebrafish as a model. METHOD(S): We used morphometric and behavioral analyses to study development of the ear and check hearing and spatial orientation in Kcnb1 mutants and morphants. Using qRT‑PCR we checked level of expression of ear marker genes. Immunohistochemistry was used to stain cilia of the hair cells. RESULTS: Ears of developing zebrafish kcnb1‑/‑ are affected resulting in abnormal morphology and function. Mutants show a significant reduction in size of ears and otoliths as compared to wild‑types. We found changes in the morphology of the hair cells in the ear of kcnb1‑/‑ with changes in orientation of kinocilia. Upregulation and downregulation of some of ear marker genes has been confirmed. Behavioral tests showed defects in hearing and balance in kcnb1‑/‑ and knockdowns. CONCLUSIONS: Our results support a hypothesis that Kcnb1 is important during development and function of the zebrafish ear.
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Przyżyciowe badania nad funkcjonowaniem mózgu przy użyciu mikroskopii SPIM

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Bazala M., Jedrychowska J., Kuznicki J.
Wszechświat
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2016
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tom 117
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nr 10-12
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Voltage-gated potassium channel Kcnb1 plays a role in development of hearing and vestibular function in zebrafish

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Jedrychowska J., Kuznicki J., Korzh V.
Acta Neurobiologiae Experimentalis
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2017
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tom 77
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nr Suppl.1
INTRODUCTION: Voltage-gated potassium channels selectively regulate transport of potassium ions along electrochemical gradient in plasma membrane. They are involved in many crucial biological processes in both excitable and non-excitable cells, including action potential, apoptosis, cell proliferation and differentiation, neurotransmitter or hormone release and cardiac activity. Hence, their dysfunctions lead to severe human disorders, e.g. deafness or epilepsy. In our study we focused on the zebrafish Kcnb1 (Kv2.1), a member of the Kv2 subfamily of voltage-gated potassium channels. Recently, a novel function of Kcnb1 in formation of the brain ventricular system in zebrafish has been found. This study demonstrated that during development Kcnb1 is expressed also in the ear and eye. Based on its expression pattern we suggest that Kcnb1 is important for development of the ear, where it may be required for hearing and spatial orientation. AIM(S): The aim of this study was to use the zebrafish mutant of Kcnb1 to investigate a role of Kcnb1 during development of the ear. METHOD(S): We used morphometric and behavioral analyses to study development of the ear and check hearing and spatial orientation in Kcnb1 mutant embryos and larvae. RESULTS: The otic vesicle and otoliths of Kcnb1 mutant develop relatively normal. We observed significant length reduction of the otic vesicle in mutant embryos. Mutants demonstrate significant hearing defects and uncoordinated balance movements. CONCLUSIONS: Unlike clear morphological abnormality of the brain ventricular system, development of the otic vesicle in Kcnb1 zebrafish mutant is relatively uneventful. Kcnb1 requirement in the ear manifests itself in mutants as abnormal hearing and vestibular function. FINANCIAL SUPPORT: This work was supported by funds from OPUS grant to Vladimir Korzh from National Science Centre (OPUS UMO-2016/21/B/N23/00354).
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