Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 9

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Program do obsługi serwisu maszyn rolniczych

100%
Borusiewicz A., Drozyner P., Marczuk T., Winiarczyk M.
Problemy Inżynierii Rolniczej
|
2015
|
tom 23
|
nr 4
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Fusarium keratitis as a growing problem - case report and literature review

100%
Winiarczyk M., Borkowska A., Mackiewicz J.
Annals of Agricultural and Environmental Medicine
|
2021
|
tom 28
|
nr 3
3

The effect of fenitrothion on Spodoptera exigua larval tissues and the in vitro cell line: UCR-SE-1

100%
Adamski Z., Fila K., Winiarczyk M., Ziemnicki K.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
4

Azot bez strat

88%
Zalewska K., Jadwisienczak K., Cycan D., Winiarczyk M., Zarajczyj J.
Farmer
|
2012
|
nr 07
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

88%
Banach T., Adaszek L., Wylupek D., Winiarczyk M., Winiarczyk S.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 3
Proteomics including the studies of the structure, function and dependences between proteins is more and more extensively applied in human medicine and veterinary medicine. The analysis of protein profiles of tissues and body fluid from healthy and ill individuals allows to identify diagnostic, prognostic and predictive markers in various pathological states in people and animals. This paper presents preparation of urine samples for analysis in the mass spectrometer MALDI-TOF (Ultraflextreme, Bruker, Bremen, Germany) by means of two methods: liquid chromatography based on the system Nano-LC (PROTEINER FC II, Bruker Daltonics, Bremen Germany). and two-direction electrophoresis 2DE (GE Healthcare, United Kingdom). Both methods enable separation of the mixture under consideration into individual fractions of high purity indispensable for obtaining readable mass spectra. The purpose of this paper is to determine applicability of these methods in analysis of protein composition of urine samples.
6

Utility of urinary markers in the assessment of renal dysfunction in familial glomerulonephritis in Dobermann dogs

88%
Winiarczyk D., Adaszek L., Madany J., Winiarczyk M., Winiarczyk S.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 1
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Occurence of Bordetella bronchiseptica in domestic cats with upper respiratory tract infections

76%
Garbal M., Adaszek L., Lyp P., Frymus J., Winiarczyk M., Winiarczyk S.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 2
Bordetella bronchiseptica is a widespread Gram-negative pathogen occurring in different mammal species. It is known to play a role in the etiology of infectious atrophic rhinitis of swine, canine kennel cough, respiratory syndromes of cats, rabbits and guinea pigs, and sporadic human cases have also been reported. The aim of this article is to present the occurrence of infections caused by these bacteria in domestic cats with respiratory symptoms, as well as to conduct a molecular analysis of the flaA gene B. bronchiseptica for the purpose of ascertaining whether cats become infected with one or more bacteria strains. B. bronchiseptica was isolated from the respiratory system of 16 out of 35 domestic cats with symptoms of respiratory tract infections. Polymorphism analysis of polymerase chain reaction products of B. bronchiseptica flaA was performed to reveal the possible differences in nucleotide sequences of the flagellin gene. The phylogenetic analysis of nucleotide sequences obtained during PCR indicated that the isolates of bacteria from our own studies are characterised by 100% homology of the analysed fragment of the flaA gene, which suggests maintenance of a single genotype of these microorganisms in the cat population. Moreover, the bacteria revealed full homology with reference strain B. bronchiseptica ATCC 4617, and 99.4% homology with strain B. parapertussis ATCC 15311. This indicates that the PCR optimised for the Bordetella spp. flaA gene, combined with sequencing of amplicons obtained in PCR, is an effective diagnostic method allowing differentiation of Bordetella spp. type microorganisms.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The usefulness of briquettes and pellets from selected plant materials for energy purposes

64%
Kowalczyk J., Zarajczyk J., Sobczak P., Mazur J., Rudy S., Cycan D., Winiarczyk M., Jadwisienczak K., Zalewska K.
Teka Komisji Motoryzacji i Energetyki Rolnictwa
|
2012
|
tom 12
|
nr 2
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Novel potential of pancreatic-like enzymes of microbial origin in exocrine pancreatic insufficiency - study on a pig model

52%
Szwiec K., Goncharova K., Velverde-Piedra J. L., Ushakova G., Kovalenko T., Osadchenko I., Kardas M., Swieboda P., Podgurniak P., Winiarczyk M., Grochowska-Niedworok E., Lozinska L., Filip R., Pierzynowski S. G.
Journal of Pre-Clinical and Clinical Research
|
2015
|
tom 09
|
nr 1
Introduction. The standard porcine-derived pancreatic enzyme replacement therapy (PERT) is a lifesaving treatment for patients with diseases causing exocrine pancreatic insufficiency (EPI). An attempt to replace PERT with microbial enzymes were undertaken. The aim was to highlight whether the mode of application, mixed with food or applied directly to the stomach, of pancreatic-like enzymes of microbial origin (PLEM) can affect their activity along the gastrointestinal tract. Materials and method. The activity of amylase, lipase and proteinase in the stomach, duodenum and ileum were tested in EPI pigs (n=6) after supplementation of PLEM, either orally – before and during feed consumption – or via the stomach – before and during feed consumption. Healthy pigs not treated with PLEM (n=3) served as controls. Activity of the enzymes measured in the chyme were obtained together with the digesta pH. Activity of the enzymatic residues in the stool samples was also checked. Results. The highest pancreatic enzyme activities were found in the duodenum of the healthy pigs (amylase 162,68 kU/ mL, lipase 507,34 kU/mL and protolitic (trypsin) activity 357,60 kU/mL). Nevertheless, the microbial enzymes remained also active along the entire length of the GIT – including stomach in EPI pigs, regardless of their route of administration. However, activity level was significantly lower. Discussion. Results indicate that the activity pattern of PLEM in the small intestine mimics the activity of the natural endogenous pancreatic enzymes in healthy pigs. The most physiological features of PLEM were observed when enzymes were offered orally. The magnitude of PLEM activity in the stomach of EPI pigs was essential and significantly higher than that measured in healthy pigs, thus being somewhat not physiological, and for health reasons of the patients should be further explored. Interestingly, specific trypsin-like activity was measured in all parts of the GIT after PLEM application. However, proteolytic activity of the experimental proteaze in in vitro studies did not exhibit trypsin-like activity.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.