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1

A note: an approach to estimate efficiency of energy utilization for milk production

100%
Chwalibog A.
Journal of Animal and Feed Sciences
|
1995
|
tom 04
|
nr 1
63-67
2

Effect of high doses of L-ascorbic acid on the antioxidative/oxidative state in the rats

51%
Niemiec T., Sawosz E., Chwalibog A.
Journal of Animal and Feed Sciences. Supplement
|
2005
|
tom 14
|
nr 1
3

Vitamin C affects the antioxidative/oxidative status in rats irradiated with ultraviolet (UV) and infrared (IR) light

51%
Niemiec T., Sawosz E., Chwalibog A.
Journal of Animal and Feed Sciences. Supplement
|
2006
|
tom 15
|
nr 1
4

High L-ascorbic acid content of diet modulates the non-specific immunity in rats

51%
Niemiec T., Sawosz E., Grodzik M., Chwalibog A.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 2
149-157
This study was designed to examine the effect of high dose of L-ascorbic acid (L-AA) as a dietary supplement for rats on the phagocytic activity and oxidative burst of non-specific immune cells. Thirty Wistar rats (225-245 g initial body weight) were divided into three groups fed diet supplemented with 0.0, 0.3, and 0.6% L-AA (0, 187 and 375 mg L-AA/kg feed) for 41 days. At the end of the experiment, blood samples were analysed for selected indicators of non-specific immunity. The application of 0.6% of LAA, as compared to the remaining two groups reduced phagocytic activity measured as a number of opsonized E. coli cells consumed by neutrophiles and monocytes. However, 0.6% of dietary AA supplementation led to increased phagocytic monocytes and neutrophiles percentage,which produced reactive oxygen species after stimulation with opsonized bacteria (E. coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP).These results indicate that megadose (0.6%) of dietary AA can reduce an oxygen-independent phagocytosis, but increase the number of neutrophiles and monocytes representing the oxygendependent mechanism of killing.
5

Effect of genetic line on energy and nitrogen metabolism during late gestation in twin-pregnant ewes fed restrictedly

51%
Kiani A., Chwalibog A., Nielsen M. O., Tauson A. H.
Journal of Animal and Feed Sciences. Supplement
|
2004
|
tom 13
|
nr 1
6

Incorporation of nitrogen isotope 15N into liver DNA during avian embryogenesis. A new approach for measuring the rate of DNA synthesis

45%
Chwalibog A., Metges C. C., Sawosz Chwalibog E., Grodzik M., Niemiec T.
Journal of Animal and Feed Sciences
|
2010
|
tom 19
|
nr 2
269-276
The objective of this experiment was to test the possibilities of measuring the rate of DNA synthesis in chicken embryos by applying a simple 15N tracer technique. We hypothesized that the rate of 15N incorporation into liver DNA depends on the type of labelled substance, reflecting precursor availability to provide substrates for nucleotide synthesis. Fertilized eggs were divided into 4 groups (4 × 15): control – not treated, and treated with 15N labeled glycine, ammonium chloride, or sodium nitrate. 15N labeled solutions were given in ovo by injection into albumen. After 20 days of incubation, the labeled substances had no effect on embryo development or morphology. Hepatic DNA was purified and 15N abundance was measured by isotope ratio mass spectrometry. There was significant enrichment of 15N in DNA from the glycine and ammonium chloride groups. We conclude that this simple technique of injecting 15N tracers into incubating eggs can be used to estimate the rate of DNA synthesis.
7

Influence of nanoparticles of gold on chicken embryos' development

45%
Zielinska A. K., Sawosz E., Grodzik M., Chwalibog A., Kamaszewski M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2009
|
tom 46
Influence of nanoparticles of gold on chicken embryos' development. The objective of this study was to examine potential effects of gold nanoparticles' hydrosols on growth and development of chicken embryo. Fertilized eggs (n = 120) were divided into 3 groups and injected in ovo; group I (control) - no treated; group II (placebo- 1) - physiological saline with 1 injection; group III (NanoAu) - hydrocolloid of Au nanoparticles - with 1 injection. Nanoparticles of Au alloy did not influence chicken embryo development. Injection in ovo with physiological saline increased level of lactate dehydrogenase in blood senim and decreased embryos' body weigh.
8

Effect of Nutrition on oxidative stress

45%
Sawosz E., Chwalibog A., Niemiec T., Kosieradzka I., Skomial J.
Journal of Animal and Feed Sciences. Supplement
|
2005
|
tom 14
|
nr 1
9

The effect of dietary energy concentration on the hormone profile and lipid metabolism in growing pigs

45%
Sawosz E., Chwalibog A., Skomial J., Kosieradzka I., Ziecik A. J.
Journal of Animal and Feed Sciences. Supplement
|
2005
|
tom 14
|
nr 1
10

Influence of nanoparticles of silver/palladium alloy on chicken embryos' development

45%
Studnicka A., Sawosz E., Grodzik M., Chwalibog A., Balcerak M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2009
|
tom 46
Influence of nanoparlicles of silver/palladium alloy on chicken embryos' development. The objective of this study was to examine potential effects of silver/palladium alloy nanoparticles' hydrosols on growth and development of chicken embryo. Fertilized eggs (n = 75) were divided into 5 groups and injected in ovo; group 1 (control) - no treated; group II (placebo-1) physiological saline with 1 injection; group III (Ag/Pd-1) - hydrocolloid of Ag/Pd nanoparticles with 1 injection; group IV (placebo-2) - physiological saline with 2 injections; group V (Ag/Pd- 2) - hydrocolloid of Ag/Pd nanoparticles with 2 injections. Nanoparticles of Ag/Pd alloy did not influence chicken embryo development. Injection in ovo with physiological saline increased activity of asparagine transferase in blood serum and caused hypertrophy of hepatocytes. Injection of nanoparticles Ag/Pd showed tendency to restore this negative effect.
11

Taurine protects DNA of lymphocytes against oxidative alteration in riding horses

39%
Sokol J. L., Sawosz E., Niemiec T., Majchrzak K., Chrzanowski S., Lojek A., Chwalibog A.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 1
33-40
The study aimed at evaluation the effect of dietary supplement of taurine on the oxidation-reduction status in riding horses, and especially on the extent of oxidative DNA degradation in lymphocytes.Ten Thoroughbred and half-bred geldings aged 6-13 years were classified according to breed and amount of work done into two groups – control (C, n=5) and experimental (E, n=5), the latter fed the diet with addition of 40 g taurine/horse/day. Blood samples were withdrawn from the horses’jugular vein before commencing the riding season and then after 30 days of working. In the blond some selected morphological and biochemical indicators were determined including the TBA-RS and 8-oxo-dG in lymphocyte DNA. It was found that physical effort of horses, being used for 30 days in recreation riding, affected homeostasis of redox status, and especially the rate of oxidative DNA degradation of lymphocytes. The addition of taurine to feed caused smaller oxidative stress,manifested by lower concentration of TBA-RS in plasma and of 8-oxo-dG in lymphocytes. The taurine lowered the lipid peroxidation intensity that occurred in horses due to the oxidative stress caused by physical effort. Furthermore, taurine revealed affinity to nucleic acids, counteracted the oxidative DNA degradation and decreased the damages of DNA in lymphocytes.
12

Oxidation of carbohydrates and fat in newly hatched chickens

39%
Chwalibog A., Tauson A.-H., Matthiesen C., Ali A., Thorhauge K., Sawosz E., Thorbek G.
Journal of Animal and Feed Sciences. Supplement
|
2004
|
tom 13
|
nr 2
13

Phagocytic and oxidative-burst activity of blood leukocytes in rats fed a protein-free diet

39%
Sawosz E., Winnicka A., Chwalibog A., Niemiec T., Grodzik M., Sikorska J.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 4
The objective of this study was to evaluate the effects of two weeks' protein deprivation on the cellular parameters of non-specific immunity in rats. Wistar rats (200-250 g) were divided into two groups (2x12) and were fed two isoenergetic (control and protein-free) diets. The phagocytic activity of neutrophils and monocytes, and the oxidative-burst activity of neutrophils of peripheral blood, were determined by flow cytometry after stimulation with E. coli and phorbol 12-mirystate 13-acetate. Feeding the protein- free diet for two weeks did not influence the phagocytic activity of neutrophils, monocytes or blood morphology. However, the oxidative burst of stimulated neutrophils was increased, indicating that two weeks' protein deprivation does not depress the oxygen- dependent killing mechanism in neutrophils, but may lead to the overproduction of reactive oxygen species.
14

Effects of substitution of dietary protein with carbohydrate on lactation performance in mink [Mustela vision]

39%
Fink R., Tauson A.-H., Chwalibog A., Hansen N. E., Kristensen N. B., Wamberg S.
Journal of Animal and Feed Sciences
|
2004
|
tom 13
|
nr 4
647-664
15

Nano-nutrition of chicken embryos. Effect of gold and taurine nanoparticles on muscle development

39%
Zielinska M. K., Sawosz E., Chwalibog A., Ostaszewska T., Kamaszewski M., Grodzik M., Skomial J.
Journal of Animal and Feed Sciences
|
2010
|
tom 19
|
nr 2
277-285
The objective of present experiment was to reveal influence of taurine, nanoparticles of gold or nanoparticles of gold conjugated with taurine on molecular and morphological status of muscle’s fibre proliferation activity at the end of embryogenesis. The expression of Proliferating Cell Nuclear Antigen (PCNA) was determined in embryonic chicken pectoral muscle using real time PCR and immunohistochemical method. Furthermore, we calculated the average number of nuclei within the muscle fibre fasciculus in cross sectional image. Taurine, supplemented in ovo to chicken embryo, enhanced organization of breast muscle by activating molecular mechanisms evaluated as an expression of PCNA. Nanoparticles of gold did not influence examined parameters.
16

Effect of vitamins EplusC and taurine on the oxidative state of DNA in the liver of growing pigs

39%
Sawosz E., Strawa A., Chwalibog A., Niemiec T., Kosieradzka I., Dudkowska I.
Journal of Animal and Feed Sciences. Supplement
|
2004
|
tom 13
|
nr 2
17

Can silver nanoparticles affect the mineral content, structure and mechanical properties of chicken embryo bones?

39%
Sikorska J., Szmidt M., Sawosz E., Niemiec T., Grodzik M., Chwalibog A.
Journal of Animal and Feed Sciences
|
2010
|
tom 19
|
nr 2
286-291
Biomedical application of silver nanoparticles has recently gained much attention. In this study, we investigated whether hydrocolloids of silver nanoparticles (nano-Ag) are deposited in chicken bones during embryogenesis and to what extent they can affect biochemical characteristics, mineral content, structure, and mechanical properties of bones. Hydrocolloids (0.3 ml), containing 50 ppm of nano-Ag, were injected in ovo prior to incubation of eggs. After 20 days of incubation, blood and thigh bones were isolated and analysed. Nano-Ag was deposited in embryo thigh bones, but did not affect the structure or mechanical properties of the bone. There was no effect of nano-Ag on the selected biochemical indices, but there was a tendency towards increasing mineral content, indicating that nanoparticles may influence bone mineralization. Considering that nano-Ag is absorbed by the embryo skeleton without affecting bone properties, these particles might be a good candidate for carriers of micronutrients or drugs into bones.
18

Influence of hydrocolloids of Ag, Au, and Ag-Cu alloy nanoparticles on the inflammatory state at transcriptional level

33%
Sawosz E., Grodzik M., Lisowski P., Zwierzchowski L., Niemiec T., Zielinska M., Szmidt M., Chwalibog A.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 1
81-85
The objective of this investigation was to evaluate the pro- or anti-inflammatory properties of nanoparticles of Ag, Au, and Ag/Cu alloy by examining the expression of NF-κB mRNA. The experiment was performed in ovo, on the chicken embryos' model. The nanoparticles had no effect on embryos' survival; the embryos from all groups were properly developed, without any abnormalities. Contrary to Ag and Au, nanoparticles of Ag/Cu increased NF-κB mRNA expression in embryo liver, indicating a proinflammatory effect. After treatment with LPS there was a significant decrease in NF-κB mRNA expression in the liver of embryos treated with Ag, compared to the placebo, Au, and Ag/Cu groups, indicating that Ag nanoparticles act as a potential anti-inflammatory factor. The results indicate the lack of influence of Ag and Au nanoparticles on NF-κB mRNA expression in chicken embryo liver. Contrary to Ag and Au, nanoparticles of Ag/Cu alloy may be considered as a pro-inflammatory factor. Nanoparticles of Ag, but not Au and Ag/Cu, can prevent over-expression of NF-κB mRNA after LPS stimulation.
19

Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

33%
Jaworski S., Sawosz E., Grodzik M., Kutwin M., Wierzbicki M., Wlodyga K., Jasik A., Reichert M., Chwalibog A.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 4
The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and l M 18 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.
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