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1

Morphology and ultrastructure of kiwifruits resulting from artifical pollination

100%
Abreu I., Oliveira M., Veloso A.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Important phytopathogenic airborne fungal spores in a rural area: incidence of Botrytis cinerea and Oidium spp.

100%
Oliveira M., Guerner-Moreira J., Mesquita M. M., Abreu I.
Annals of Agricultural and Environmental Medicine
|
2009
|
tom 16
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nr 2
197-204
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Annual variation of fungal spores in atmosphere of Porto: 2003

100%
Oliveira M., Ribeiro H., Abreu I.
Annals of Agricultural and Environmental Medicine
|
2005
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tom 12
|
nr 2
309-315
The seasonal distribution of fungal spore concentration in the city of Porto, Portugal, was continuously studied, from 1 January to 31 December 2003, using a 7-day volumetric Burkard trap. In Portugal, aerobiological studies are scarce, and to our knowledge there are no published data on the atmospheric concentration of fungal spores. The aim of this work therefore is to initiate the aeromycological studies in Porto. The total airborne spore concentration fluctuated between 2 and 2,198 spores/m3 per day with an daily mean of 403 spores/m3. The highest airborne spore concentration was found during the summer and the early autumn, while the lowest concentration was registered during the winter. A range of allergenic and phytopathogenic fungal spores was present in the atmosphere of Porto throughout the entire year, although in different concentrations. Among the 22 fungal spore types identified Cladosporium (74.5%), Ganoderma (11.7%), Aspergillaceae (2.9%), Ustilago (2.5%), Coprinus (1.5%), Alternaria (1.3%) and Botrytis (1.3%) were the most frequent. Seasonally, spore levels of Cladosporium and Alternaria peaked in the autumn and winter, Ganoderma peaked toward autumn, whilst spore levels of the basidiomycete Coprinus fluctuated throughout the year. The total spore concentration was negatively correlated with wind speed and positively correlated with temperature and relative humidity.
4

Differential gene expression analysis during embyrogenesis in maritime pine

100%
Goncalves S., Cairney J., Oliveira M., Miguel C.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
5
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Biofilm - formation by Staphylococcus aureus and Staphylococcus epidermidis isolates from subclinical mastitis in conditions mimicking the udder environment

88%
Seixas R., Varanda D., Bexiga R., Tavares L., Oliveira M.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
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nr 4
Staphylococcus is the genus most commonly isolated from bovine mastitis in many countries. It may express several virulence factors including biofilm formation, which may protect the bacterial community from antimicrobials’ action, preventing these compounds from reaching its interior, where they reach subinhibitory concentrations (subMIC). Most biofilm production assays are performed in static conditions, while studies regarding antimicrobial resistance usually do not resemble the udder environment because they are performed at high concentrations. In this study we evaluated the influence of dynamic conditions and media, including Mueller Hinton Broth (MHB) and UHT whole milk (WM), as well as the effect of subMIC concentrations of five different antimicrobial agents on biofilm formation by staphylococci isolated from subclinical mastitis. Results suggest that dynamic conditions and media may influence biofilm formation and revealed that milking simulation may significantly increase biofilm production. Sub- MIC concentrations decrease biofilm formation in MHB but increase in WM, suggesting a protective role of milk against antimicrobial compounds’ action. Therefore, in vitro conditions that simulate the udder environment and in vivo conditions should be included as one of the parameters in evaluation of biofilm producing strains, in order to provide more reliable results.
6
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Forecasting ARIMA models for atmospheric vineyard pathogens in Galicia and Northern Portugal: Botrytis cinerea spores

76%
Fernandez-Gonzalez M., Rodriguez-Rajo F. J., Jato V., Aira M. J., Ribeiro H., Oliveira M., Abreu I.
Annals of Agricultural and Environmental Medicine
|
2012
|
tom 19
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nr 2
Botrytis cinerea is the cause of the most common disease in the Galician and Portuguese vineyards. Knowledge of the spore levels in the atmosphere of vineyards is a tool for forecasting models of the concentration of spores in order to adjust the phytosanitary treatments to real risk infection periods. The presented study was conducted in two vineyards, one located in Cenlle (Spain) and other in Amares (Portugal), from 2005-2007. A volumetric trap, model Lanzoni VPPS-2000, was used for the aerobiological study. Phenological observations were conducted on 20 vines of three grape varieties in Cenlle (Treixadura, Godello and Loureira) and in Amares (Trajadura, Loureiro and Pedernã), by using the BBCH scale. The highest total spore concentrations during the grapevine cycle were recorded in 2007 in both locations (Cenlle:16,145 spores; Amares:1,858 spores), and the lowest, in 2005 in Cenlle (1,700 spores) and in Amares (800 spores) in 2006. In Cenlle, the best adjusted model was an ARIMA (0,2,2), including the relative humidity four days earlier, while in Amares there was an ARIMA (1,2,3), considering the relative humidity three days earlier and rainfall two days earlier. The t-test showed no significant difference between observed and predicted data by the model.
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