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The present study was intended to facilitate ex situ conservation of Paphiopedilum venustum, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g Lˉ¹)] and inorganic [banana homogenate (10, 20, 30 g Lˉ¹) and potato powder (5.0, 10 g Lˉ¹)] growth supplements. Peptone at 1.0 g Lˉ¹ was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.
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Renin angiotensin aldosterone system (RAAS) is a hormone regulatory hormone system that regulate blood pressure. The two major genes ACE and AGT are the players of RAAS pathway. These genes codes for angiotensin convertase enzyme and angiotensinogen protein respectively. The angiotensin convertase enzyme convert inactive angiotensinogen into active angiotensin which further helps in the regulation of blood pressure. Due to imbalance in this pathway may cause hypertension. So in the present study we decided to perform the computational study of ACE and AGT gene. We evaluated the deleterious/damaging effect of SNPs of ACE and AGT gene by SIFT and I-Mutant2.0. The total number of SNPs predicted to be deleterious by both tools were 5 (1.83%) and 22 (6.07%) for AGT and ACE genes respectively. We also studied subcellular location of ACE and AGT genes and drugs targeting these genes from database GeneCards. Further the result output of both the softwares were also compared.
The mechanism imparting thermotolerance by salicylic acid (SA) and abscisic acid (ABA) is still unresolved using either spraying technique or in vitro conditions. Alternative way of studying these effects under near in vivo conditions is through the use of liquid culturing technique. Effects of SA and ABA (100 µM) on antioxidative enzymes, antioxidants and lipid peroxidation were studied in detached tillers of three wheat (Triticum aestivum L.) cultivars PBW 343, C 306 (heat tolerant) and WH 542 (heat susceptible) cultured in a liquid medium. Ears were subjected to heat shock treatment (45°C for 2 h) and then maintained at 25°C for 5 days. Heat shock treatment resulted in increased peroxidase (POD) activity, while superoxide dismutase (SOD) and catalase (CAT) activities were reduced compared to control. The decrease in CAT activity was more significant in susceptible cultivar WH 542. Concomitantly, content of α-tocopherol and lipid peroxides increased in heat-treated wheat ears, whereas contents of total ascorbate level were reduced. Following treatment with SA and ABA, activities of all three antioxidative enzymes increased in correspondence with an increase in ascorbate and α-tocopherol content. Apparently, lipid peroxide content was reduced by SA in heat tolerant cultivars (PBW 343 and C 306) whereas in susceptible cultivar it was decreased by ABA. The up-regulation of the antioxidant system by SA and ABA possibly contributes to better tolerance against heat shock-induced oxidative damage in wheat grains.
We investigated the dynamics of phytotoxicity and the quantitative changes in phenolics in decomposing root residues of Ageratum conyzoides over a 60-day period. A set of four treatments, viz. residues alone (R), residues mixed into soils (R + S, R + 3S), and soil alone (S), were maintained and the quantitative changes in phytotoxicity were monitored on 1, 5, 10, 15, 20, 25, 30, 45, and 60 day after decomposition (DAD). The phytotoxicity during the decomposition process was evaluated in a laboratory bioassay against radish (Raphanus sativus). The phytotoxicity of R, R + S, and R + 3S treatments increased during initial period of decomposition (up to 20-DAD), and declined afterwards (i.e., at >20-DAD). In general, the phytotoxicity was in the order R > R + S > R + 3S treatments. It was paralleled by a similar trend of changes in the amounts of water-soluble phenolics that increased up to 20-DAD and thereafter declined. The amount of phenolics was in the order R > R + S > R + 3S. At 1-DAD, the amount of water soluble phenolics in R, R + S, and R + 3S treatments was 765.3, 594.5, and 251.3 µg/ml, respectively. It enhanced to 1,266.76, 845.5, and 416.0 µg/ml at 20-DAD. However, at 60-DAD, the amounts of phenolics in R, R + S, and R + 3S treatments was 149.93, 142.6, and 100.0 µg/ml, respectively. The study concludes that the phytotoxicity of below-ground residues of A. conyzoides changes during decomposition andwas reduced upon the addition of soil to the residues.
Chickpea (Cicer arietinum L.) genotypes are sensitive to low temperature (<10°C) during its reproductive stage suffer from abortion of flowers, infertile pods and small shriveled seeds that resulted in a significant decrease in crop yield. In the present investigation seeds of a number of cold stress-tolerant and susceptible genotypes were evaluated for biochemical and molecular diversity with the purpose to categorize them. The activities of various antioxidative enzymes (superoxide dismutase, glutathione reductase, ascorbate peroxidase and catalase), content of H₂O₂ and malondialdehyde, enzymes involved in phosphate metabolism (acid and alkaline phosphatases), and content of phytic acid and proline were determined in seeds of 20 cold stress tolerant and seven cold stress susceptible genotypes. Higher activities of superoxide dismutase, ascorbate peroxidase, catalase and acid phosphatase and low content of malondialdehyde and phytic acid were observed in cold stress-tolerant genotypes as compared to cold stress susceptible genotypes. Seventeen chickpea genotypes comprising both cold stress-tolerant and susceptible ones were evaluated through 20 randomly amplified polymorphic DNA (RAPD) primers. The results of cluster analysis revealed two major groups. In the first group five tolerant (group 1a) and six susceptible genotypes (group 1b) clustered together whereas in second group all the tolerant genotypes clustered together (group 2). Out of 20 RAPD primers, 4 primers (Opa-13, Opa-14, Opa-15 and Opa-16) have been identified as markers for cold stress tolerance. In general high SOD activity, and H₂O₂ content and low MDA and phytic acid content are related with cold stress tolerance. The status of these markers was more pronounced in genotypes clustered in group 2 after RAPD analysis than in group 1a of cold stress-tolerant genotypes as compared to susceptible genotypes. The observed biochemical and molecular diversity could be useful for identifying and developing cold stress-tolerant genotypes of chickpea.
The cycloid scales reveal the presence of a distinct larval mark under the scanning electron microscope. A linear relationship between total fish length and lateral scale radius was observed. The exponent of the length-weight relationship was 2.519 and 3.094, the harvestable size 29.7 and 26.0 cm (total length), and the ultimate size based on Walford’s graph 76.0 and 64.5 cm in Gobindsagar Reservoir (Himachal Pradesh), and Harike Wetland (Punjab) respectively.
Six genotypes and thirty-eight advance breeding lines of lentil (Lens culinaris Medik.) were evaluated for carbohydrate composition, soluble proteins, mineral content and antinutritional traits such as phenolic compounds, tannins, trypsin inhibitors, saponins and phytic acid. The average content of total sugars, starch and proteins was found to be 47.18, 421.2, 236.24 mg/g, respectively. Lentil genotypes contained higher content of iron, followed by zinc and the least content of copper. The content of bound fructose, phytic acid, tannins and phenols showed significant variation in lentil genotypes. Seven genotypes namely LL-1277, LL-1302, LL-1306, LL-1310, LL-1317, LL-1325 and L-4147 have low phytic acid content and are nutritionally good. LL-1231, LL-1305 and LL- 1317 had higher content of both zinc and iron as well as protein content. LL-1328 could be important for pest resistance due to presence of higher trypsin inhibitor activity. Antioxidant potential of these genotypes estimated on the basis of free radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), total reducing power, hydroxyl radical scavenging activity, superoxide anion radical scavenging activity and nitric oxide radical scavenging activity showed significant variation among genotypes. Thirteen genotypes (LL-699, LL-1209, LL- 1223, LL-1277, LL-1279, LL-1302, LL-1304, LL-1306, LL-1309, LL-1311, LL-1315, LL-1321, LL-1325) showed high antioxidant activity. Advance breeding lines namely LL-1161, LL-1221, LL-1313, LL-1325, L-4147 are nutritionally important with high protein content, low/medium antinutritional factors, high antioxidant potential and good yield.
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