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The effect of fruit maturity on UV-B-induced post-harvest anthocyanin accumulation in red Chinese sand pear (Pyrus pyrifolia Nakai) cultivar ‘Mantianhong’ was evaluated. During the irradiation, compared with the fruit harvested at 20 days before harvest (DBH) and 10 DBH, the mature fruit (harvested at commercial harvest date) had higher soluble solids content, soluble sugars concentration but lower firmness and starch content. In addition, higher content of anthocyanin has been detected in mature fruits than in immature fruits due to the significant increase in the expression of genes related to anthocyanin biosynthesis, especially PpCHS, PpF3H, PpANS, PpUFGT, PyMYB10 and PpbHLH in red Chinese sand pears. Hierarchical clustering analysis suggested that most genes related to anthocyanin biosynthesis showed a coordinate expression pattern. These findings are helpful in understanding the molecular mechanism of anthocyanin biosynthesis and regulation, which could lead to the development of new technologies for improving fruit color in Chinese sand pears and other fruits.
Emissions trading schemes (ETS) have been treated as a cost-effective mitigation measure to effectively control carbon emissions. Industrial carbon emission quota allocation is prior to the implementation of ETS. This study takes industrial sectors in China as a case to apportion carbon emission quotas. An informational entropy and multiple-factor mixed weighting allocation model (IEMMA) was established by considering fairness, efficiency, and feasibility from 4 aspects, i.e., emission reduction responsibility, emission reduction potential, emission reduction capacity, and industrial features. The allocation results among industrial sectors present many differences, and averaging a weighting allocation scheme is more feasible than other allocation schemes considering the fairness, efficiency, and feasibility. This study not only advances the existing literature on the issue of sectoral carbon emission quota allocation, but also provides a significant reference for China’s policymaking in ETS implementation.
The current study evaluated appetite-related gene expression of neuropeptide Y (NPY), orexin, type 2 orexin receptor (OrexinR2), proopiomelanocortin (POMC), melanocortin-4 receptor (MC4R), AMP-activated protein kinase α2 subunit (AMPKα2) and long-form leptin receptor (LeptinRb) in hypothalamus in response to copper (Cu) supplementation. One hundred crossbred pigs were assigned to four groups of 25 pigs, each comprising five replicates of 5 animals. Groups were then randomly assigned to treatments consisted of 1. control (10 mg/kg CuSO4), 2. 100 mg/kg CuSO4, 3. 175 mg/kg CuSO4, 4. 250 mg/kg CuSO4. On d 21 of the experiment 5 pigs from each group were slaughtered and the hypothalami were collected for determination of appetite-regulating genes mRNA expression level. The results showed that average daily feed intake and average daily gain were higher (P<0.05) in 250 and 175, 250 mg/kg Cu supplemented groups, respectively, than in the 10 mg/kg group. Feed:gain ratio was lower in pigs fed the diets with 250 mg/kg Cu (P<0.05) than in the 10 mg/kg group. Furthermore, the abundances of NPY mRNA in hypothalamus were higher in 250 mg/kg Cu supplemented groups (P<0.05), whereas the abundances of POMC and LeptinRb mRNA were significantly reduced (P<0.05) in 170, 250 mg/kg and 100, 175, 250 mg/kg Cu supplemented groups, respectively. No difference was found for AMPKα2, MC4R, orexin, OrexinR2 mRNA expression (P>0.05). These data suggest that down-regulation of LeptinRb mRNA expression might contribute to the stimulation of feeding of high dietary Cu supplementation via regulation of NPY and POMC mRNA expression.
Although some genes involved in heavy metal detoxification and tolerance have been identified in plants, the mechanisms underlying heavy metal resistance remain to be elucidated further. To investigate its molecular mechanisms, a forward genetic screen was performed for identifying Arabidopsis (Arabidopsis thaliana) cadmium (Cd)-sensitive mutants. Here we report a novel cms1-1 (cadmium-sensitive) mutant, which defines a genetic locus involved in the regulation of cadmium and lead (Pb) resistance in Arabidopsis. cms1-1 plants were hypersensitive to Cd but resistant to Pb as well as oxidative stress mediated by hydrogen peroxide and methyl viologen. Genetic analysis indicated that cms1-1 is a recessive mutation in a single nuclear gene. When subjected to Cd or Pb stress, higher Cd or lower Pb content was detected in cms1-1 plants than in wild-type plants, respectively. The cms1-1 mutation altered the expression pattern of some Cd/ Pb stress-related genes, including AtPDR8 and AtPDR12, which was associated with changes of Cd and Pb contents. In addition, Cd hypersensitivity/enhanced Pb resistance mediated by cms1-1 is glutathione (GSH)- independent. The results suggest that CMS1 plays distinct roles in the regulation of Cd and Pb resistance mainly through a GSHindependent AtPDR12/AtPDR8-mediated mechanism.
The aim of the studies was to develop an alternative method which could overcome the lack of sampling to improve the efficiencx of control efforts for bovine endemic fluorosis. The spatial distribution characteristics of the disease were analysed and a prediction model for the estimation of fluorosis distribution in some districts in northwest Liaoning province in China was established. The model used ordinary kriging, and was evaluated using cross-validation. Analysis showed that the distribution of the disease was spatial autocorrelation. The prediction error of the cross-validation (ME = -0.0092, PMSE = 0.627, AKSE = 0.597, and RMSP = 1.007) and comparison with the actual disease distribution indicated that the prediction map accurately distributed bovine endemic fluorosis. It is feasible to predict bovine endemic fluorosis in the area by using ordinary kriging and limited data.
The objective of the study was to evaluate the effects of increasing dietary standardized ileal digestible (SID) lysine (Lys) level on the expression of jejunal amino acids (AAs) transporters and the microflora in the hindgut of weaned pigs. One hundred and twenty weanling pigs weighing 8.10 ± 0.48 kg were randomly assigned according to body weight and sex to 5 treatments with 6 replicates per treatment and 4 pigs per replicate. Pigs were fed diets with 0.98, 1.11, 1.23, 1.35 or 1.48% of SID Lys for 28 days. The mRNA expression of cationic amino acids transporter 1 (CAT1) in jejunum was higher in groups fed with 1.23, 1.35 and 1.48% SID Lys addition (P < 0.05). There was stated a linear increase in the mRNA expressions of CAT1, excitatory amino acids carrier 1 (EAAC1) and peptide transporter T1 (PEPT1) (linear, P < 0.05). In the caecum, the populations of bacteria and the content of butyric acid were significantly influenced (P < 0.05) by dietary SID Lys. In animals fed diet with 1.35% SID Lys a content of butyric acid was the highest (P < 0.05). Moreover, the populations of Lactobacillus and Bifidobacterium in caecum and colon increased (linear, P < 0.05; quadratic, P < 0.05) as dietary SID Lys level increased. The obtained results showed that dietary SID Lys level may influence AAs absorption and promote the hindgut health. The suggested supplementation of SID Lys to the weaned pigs’ diet is 1.35%.
The objective of this study was to evaluate the effect of different fiber sources on jejunal digestive and absorptive physiology in weaned piglets. One hundred weaned piglets were allotted according to body weight, gender and litter to four dietary treatments. Each treatment was replicated in 5 pens of 5 pigs each. The experimental diets contained 10% of a fiber source: wheat bran (WB), maize fiber (MF), soyabean fiber (SF), or pea fiber (PF). Piglets were fed ad libitum for 30 d. Reduced villus height and villus height-to-crypt depth ratio (P < 0.05) were observed in pigs fed diet MF compared with pigs fed diet WB. In the mid-jejunal mucosa, lower activities of sucrase (P < 0.05) were found in pigs fed diets MF and SF compared with WB. In mid-jejunal digesta, inclusion of SF and PF decreased the activities of trypsin and lipase (P < 0.05) compared with inclusion of WB. Supplementation with PF resulted in higher facilitated glucose transporter 2 (P < 0.05) and lower excitatory amino acid carrier 1 mRNA levels compared with supplementing WB. The apparent faecal digestibility of gross energy, dry matter, organic matter, neutral detergent fiber and acid detergent fiber declined in the following order: diet SF > PF > MF > WB. Our study indicates that SF and PF could promote apparent faecal digestibility in piglets, but this is not accompanied by better jejunal morphology or digestive enzyme activities as compared with WB.
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