Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 56

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Biosynthesis of raffinose family oligosaccharides and galactosyl pinitols in developing and maturing seeds of winter vetch [Vicia villosa Roth.]

100%
Lahuta L. B.
Acta Societatis Botanicorum Poloniae
|
2006
|
tom 75
|
nr 3
219-227
Changes in the accumulation of two types of α-D-galactosides: raffinose family oligosaccharides and galactosyl pinitols were compared with changes in the activities of galactosyltransferases during winter vetch (Vicia villosa Roth.) seed development and maturation. Occurrence of galactinol and raffinose in young seeds and changes in activities of galactinol synthase and raffinose synthase during seed development indicated that formation of raffinose oligosaccharides (RFOs) preceded synthesis of galactopinitols. Although transfer of galactose residues into raffinose oligosaccharides increased as seeds were maturing, at late stages of seed maturation the accumulation of galactopinitols was preferred to that of RFOs. In the present study, activities of enzymes transferring galactose moieties from galactinol to D-pinitol forming galactopinitol A, and further transfer of galactose moieties from galactinol to mono- and di-galactopinitol A were detected throughout seed development and maturation. This is a new observation, indicating biological potential of winter vetch seeds to synthesize mono-, di- and tri-galactosides of D-pinitol in a pathway similar to RFOs. The pattern of changes in activities of stachyose synthase and enzymes synthesizing galactopinitols (named galactopinitol A synthase and ciceritol synthase) suggests that formation of stachyose, mono- and di-galactopinitol A (ciceritol) is catalyzed by one enzyme. High correlation between activities of verbascose synthase and enzyme catalyzing synthesis of tri-galactopinitol A from galactinol and ciceritol (named tri-galactopinitol A synthase) also suggests that biosynthesis of both types of tri-galactosides was catalyzed by one enzyme, but distinct from stachyose synthase. Changes in concentrations of galactosyl acceptors (sucrose and D-pinitol) can be a factor which regulates splitting of galactose moieties between both types of galactosides in winter vetch seeds.
2

The effect of ABA and dehydration on the accumulation of raffinose family oligosacharides and galactosyl cyclitols in developing hairy tare (Vicia hirsuta L. S.F. Gray) seeds

100%
Lahuta L. B.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 3 suppl.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch [Vicia villosa Roth.] seeds

63%
Lahuta L. B., Goszczynska J.
Acta Societatis Botanicorum Poloniae
|
2009
|
tom 78
|
nr 3
203-208
Beside RFOs, which are commonly present in legume seeds, seeds of some species contain galactosyl pinitols (GPs). These carbohydrates, like RFOs, have been hypothesized to constitute an important energy and carbon skeletal source during germination. To test this hypothesis we have applied a specific α-galactosidase inhibitor (1-deoxygalactonojirimycin, DGJ) to germinating winter vetch (Vicia villosa Roth.) seeds, containing more galactosyl pinitols than RFOs. The breakdown of RFOs but not that of GPs was completely blocked in both embryonic axes and cotyledons tissues, during the first 18 h of imbibition in DGJ. The inhibitor only decreased the rate of GPs degradation. The inhibitory effect of DGJ on GPs degradation was partially alleviated by addition of sucrose or galactose to DGJ solutions. After three days of germination in water, RFOs and GPs disappeared in axial tissues of seeds imbibed in water, galactose or sucrose. Eighteen-hour imbibition of seeds in DGJ drastically reduced germination, by ca 50%, during the first three days. The inhibitory effect of DGJ decreased during the next seven days of germination. The presence of galactose or sucrose in imbibition solution initially stimulated seed germination, but later this effect was not statistically significant. Our study provides clear evidence that galactosyl pinitols play an important role in early winter vetch seeds germination. Additionally, we suggest that galactosyl pinitols can replace RFOs as reserve material necessary for early germination.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Wpływ niektórych zabiegów kulinarnych na zmiany poziomu węglowodanów rozpuszczalnych w nasionach soczewicy i ciecierzycy

63%
Wieczorek C., Lahuta L. B.
Żywność Nauka Technologia Jakość
|
2007
|
tom 14
|
nr 3
Celem badań była analiza składu węglowodanów rozpuszczalnych w nasionach soczewicy i ciecierzycy oraz ocena wpływu wybranych zabiegów obróbki kulinarnej na zmiany zawartości tych substancji w badanych nasionach. Nasiona soczewicy i ciecierzycy poddano obróbce technologicznej obejmującej moczenie i gotowanie. Badania fizykochemiczne obejmowały oznaczenie wodochłonności oraz zawartości suchej masy i węglowodanów rozpuszczalnych. Analizę otrzymanych wyników przeprowadzono w stosunku do nasion suchych bez obróbki kulinarnej. Wyjściowa średnia zawartość węglowodanów rozpuszczalnych w surowych, suchych nasionach soczewicy i ciecierzycy wynosiła odpowiednio 85,3-91,4 mg/g s.m i 98,4-109,8 mg/g s.m. W soczewicy dominowały oligosacharydy z rodziny rafinozy (38-40%), a w ciecierzycy galaktozylocyklitole (40-43%). Straty oligosacharydów z rodziny rafinozy w procesie moczenia i gotowania wynosiły w przypadku soczewicy 84%, a ciecierzycy 51%, natomiast zmniejszenie zawartości galaktozylocyklitoli odpowiednio 66 i 30%. Moczenie w porównaniu z gotowaniem spowodowało większe wymycie węglowodanów rozpuszczalnych z nasion roślin strączkowych.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Studies on soluble carbohydrates in yellow lupin [Lupinus luteus L.] seeds of different age

63%
Zalewski K., Lahuta L. B.
Acta Societatis Botanicorum Poloniae
|
2007
|
tom 76
|
nr 4
309-312
Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols), but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues). The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.
6

Desiccation tolerance of cereals grains with modified composition of alfa-D-galactosides

63%
Lahuta L. B., Goszczynska J.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1 Suppl.
7

Raffinose in seedlings of winter vetch ( Vicia villosa Roth.) under osmotic stress and followed by recovery

63%
Lahuta L. B., Gorecki R. J.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 3
During germination of winter vetch (Vicia villosa Roth.) seeds, the degradation of raffinose family oligosaccharides and galactosyl pinitols occurred faster in axis than in cotyledons. After 7 days of germination, all α-D-galactosides disappeared and the soluble carbohydrates in seedling tissues consisted of D-pinitol, sucrose, fructose, glucose and myo-inositol. Osmotic stress caused by incubation of seedlings in PEG 8000 solution (-0.5, -1.0, and -1.5 MPa) for 48 h induced the activity of crucial enzymes of the RFOs pathway, i.e. galactinol synthase and raffinose synthase, in both the root and epicotyl but not in cotyledons. The root and epicotyl accumulated elevated amounts of galactinol and raffinose as the osmotic potential was lowered. This process was transient because when PEG solution was replaced with water, galactinol and raffinose were degraded, thus confirming their direct involvement in the response of tissues to osmotic stress. Among other soluble carbohydrates, only sucrose accumulated in response to stress. The results did not show potential role of D-pinitol in the adjustment of winter vetch seedlings to osmotic stress.
8

Application of phytohormones during seed hydropriming and heat shock treatment on sunflower (Helianthus annuus L.) chilling resistance and changes in soluble carbohydrates

63%
Gornik K., Lahuta L. B.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 05
9

The metabolism of ageings seeds, changes in the raffinose family oligosaccharides during storage of field bean (Vicia faba Var. Minor Harz) seeds

63%
Zalewski K., Lahuta L. B.
Acta Societatis Botanicorum Poloniae
|
1998
|
tom 67
|
nr 2
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cyclitols in maturing grains of wheat, triticale and barley

63%
Lahuta L. B., Goszczynska J.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr 3
In the present study, the feeding of stem-flag leaf-ear explants of wheat, triticale and barley with d-chiro-inositol and d-pinitol was used for modification of the composition of soluble carbohydrates in grains without genetic transformation of plants. Maturing grains indicated ability to uptake exogenously applied cyclitols, not occurring naturally in cereal plants, and synthesized their a-d-galactosides. The pattern of changes in soluble carbohydrates during grain maturation and germination was not disturbed by the uptake and accumulation of cyclitols. Both, d-chiro-inositol and d-pinitol as well as their a-d-galactosides can be an additional pool of soluble carbohydrates accumulated by maturing grains, without decreasing seeds viability. This is the first report indicating the possibility of introduction of cyclitols with potentially human health benefits properties into cereal grains.
11

Wpływ egzogennych cyklitoli na skład cukrowców rozpuszczalnych i jakość fizjologiczną dojrzewających ziarniaków żyta ozimego (Secale cereale L. odmiany Picasso)

63%
Lahuta L. B., Goszczynska J.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 545
Badano wpływ dokarmiania dojrzewających ziarniaków żyta egzogennymi cyklitolami - D-pinitolem i D-chiro-inozytolem na akumulację cukrowców rozpuszczalnych podczas wysychania eksplantatów, desykacji ziarniaków i ich przyspieszonego starzenia się. Wykazano, że cyklitole, naturalnie nieobecne w zbożach, mogą docierać z tkanek wegetatywnych do ziarniaków, a D-chiro-inozytol może być włączany w szlak biosyntezy α-D-galaktozydów, osłabiając wytwarzanie rafinozy. Pobieranie i akumulacja cyklitoli nie wywołują zaburzeń w profilu zmian cukrowców rozpuszczalnych zarówno podczas dojrzewania jak i desykacji oraz starzenia się ziarniaków. Ziarniaki gromadzące cyklitole mogą wykazywać niższą tolerancję na szybką desykację, niż ziarniaki kontrolne. Nie wykazano istotnego wpływu cyklitoli na odporność ziarniaków na test przyspieszonego starzenia się.
12

Modifications of alpha-D-galactosides composition in legume seeds by feeding with exogenous cyclitols

63%
Lahuta L. B., Gorecki R. J.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
13

The occurrence and accumulation of d-pinitol in fenugreek (Trigonella foenum graecum L.)

51%
Lahuta L. B., Szablinska J., Ciak M., Gorecki R. J.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 08
14

What is the composition and content of soluble carbohydrates in developing and mature diaspores of Antarctic vascular plants?

51%
Kellmann-Sopyla W., Gielwanowska I., Lahuta L. B.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
15

Wplyw estru metylowego kwasu jasmonowego na formowanie polirybosomow i kielkowanie ziarna pszenzyta ozimego odmiany Ugo

51%
Widejko D., Zalewski K., Lahuta L. B., Socha A.
Folia Universitatis Agriculturae Stetinensis. Agricultura
|
2000
|
tom 82
321-324
16

Metabolite profiling of pea seedlings under desiccation stress

51%
Szablinska J., Ciak M., Lahuta L. B.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
17

The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

51%
Orlikowska T., Zawadzka M., Kucharska D., Lahuta L. B.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 2
We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4℃ in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.
18

Accumulation of raffinose family oligosaccharides in maturing seeds of pea (Pisum sativum L.)

51%
Lahuta L. B., Ciak M., Szablinska J., Pluskota W. E.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2018
|
tom 60
|
nr 1
19

The accumulation of raffinose in vegetative tissues of legume seedlings in response to cold and desiccation stress

51%
Lahuta L. B., Goszczynska J., Gorecki R. J.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
20

Changes in soluble carbohydrates in seedlings of Phaseolus vulgaris L. in response to osmotic stress

51%
Dzik T., Lahuta L. B., Gorecki R. J.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1 Suppl.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.