In Bydgoszcz and partially in Koszalin voivodeships the examinations made in small mammals (by trichinoscopic method) did not show any T. spiralis in 384 rodents from 23 species (Table 5). Toxoplasmosis was searched for by means of CFT in 584 persons (workers of State Agricultural Farms and Forestry) (Table 1); the percentage of positive results amounts to 15.5%. The use of intracutaneous test in 140 subject revealed positive findings in 25%. (Table 2). In dogs (241) the CFT test was positive in 17.01% (Table 3). Similar serologic studies in 284 small mammals showed them to be infected in 3.8% (Table 4).
Workers of a Fowl Farm and Slughter House (332 persons) were examined using complement fixation test with concurrent application of IDT in 84 subjects A positive complement fixation reaction was found in 44 persons (13.2%), slightly positive one in 9 (2.6%) and negative result in 279 subjects (84.0%). Parallel examinations by complement fixation and intradermal tests were comparable; negative results being obtained in 57 persons (67.8%). Moreover, complement fixation test was performed in cattle (64 heads) without any positive result and in 203 small mammals, where a positive result was established in 9 cases. Trichinoscopic examination for the presence of Trichinella spiralis revealed only in two cases of Sorex araneus out of 502 small mammals the occurrence of parasites, suggestive of T. spiralis. Details are given in enclosed tables.
The aim of this work was to determine the role of toxoplasmosis in the pathology of pregnancy among women in the Lublin province, and simultaneously the attempt to evaluate the correlation between three diagnostic methods: Frenkel's intradermal test, Sabin-Feldman's dye test and complement fixation reaction. 396 women were examined and 332 simultaneously submitted to serological and allergical investigations. In 64 cases the investigations were only carried out with the complement fixation and intradermal tests. The highest number of positive results was obtained by the intradernal reaction, i. e. 33.8%, the lowest by the complement fixation reaction 8.3%. The dye test gave 10.6%. The conformity of the results for all three tests amounts to 56.7% (225 cases). In the remaining cases (171) the conformity of results between the individual tests was studied, and so: for the complement fixation and the dye tests it amounted to 15.6%, for complement fixation and Frenkel's intradermal tests to 12.5%, for the dye test and Frenkel's intradermal test - 6.7%. The remaining 9,5% fell on the unconformity between the results of the complement fixation reaction and Frenkel's intradermal test. The highest percentage of infections has been observed in the group of premature deliveries; that is 56.6%. To this group belongs also a relatively great number of stillborn children and children with development defects. In the group of women who suffered abortion the percentage of positive results is the lowest – 21.05%. The percentage of positive results is almost identical in the goup of women who were not suspected of toxoplasmosis and in the group where this suspicion existed (32.1%-34,7%). An exact anamnesis concerning the pregnancies of 241 women (multiparae) has shown that the number of abortions in the group of women with positive results is by 50% higher than in the group with negative results. The number of nonviable fetuses is by 2.9 times higher and the number of premature deliveries by 30% higher. The number of infants with development defects is 3 times higher in the group of women who were infected with toxoplasmosis. The number, however, of stillborn children with positive results amounts to 3,8%, while in the group of women with negative results to 5.6%. In cases of habitual abortion, premature delivery or development defects the possibility of toxoplasma infection in the mother should be taken into consideration and laboratorial investigations carried out, such as: complement fixation reaction, Sabin-Feldman's dye test and Ftenkel's intradermal test. A positive results of even one of the serological test in comparison with clinical observations makes an anti-toxoplasmosis treatment unavoidable.
Rural children from Lublin regions were found to harbour: Trichuris trichiura in 52.5%, Enterobius vermicularis in 44.2%, Ascaris lumbricoides in 28.7% and Lamblia intestinalis in 1.1% of cases. The statistical analysis revealed a higher infection rate in boys (50.2%) than in girls (43.7%). Statistical evaluation was also concerned with the mutual correlation in the incidence of individual parasites.
287 sera were examined by means of the complement fixation reaction, applying the cold and warm method. 282 were human and 5 rabbit sera. The results of these examinations may be summarized as follows: 1) Positive results were obtained: by the warm method - 5,92%, by the cold method - 14,79%. 2) The titre with the cold method is always higher than the titre obtained with the warm method. 3) The conformity of the results of the complement fixation, Sabin-Feldman's dye test and Frenkel's intradermal test amounts to 61.5% for the warm method and to 66.6% for the cold method. The conformity of the ,results of the complement fixation and Frenkel's intradermal test amounts to 54.8% for the warm and 58.06% for the cold method. The conformity of the results of the complement fixation test and the dye test amounts to 84% for both methods.
Coprological examinations of 576 children were conducted. In 380 of the children infected were conducted examinations of the alimentary tract and in 100 control cases were conducted haematological examinations. Haematotocrit, SDE, SSHb were determined in 118 infected children and in 77 controls. The following conclusicns were drawn: 1. Rural children infected with intestinal parasites exhibit symptoms of anaemia more commonly and in a major degree (60 per cent), than the children from the same environment and of similar conditions of life, in whom no parasites were found (40 per cent). 2. The present investigations proved that the anaemia had the character of hypochromic macrocytary anaemia. 3. The changes of the white blood cells count, neutrophils and lymphocytes are too small to ascribe to sole responsibility for them to the influence of parasites. 4. An increase cosinophils count was diagnosed in 51,6 per cent of the infested children, but the normal count or even their absence do not exclude the infection of the alimentary tract with parasites. 5. The present observations did not prove that there is a major influense of the infection with intestinal parasites on the cells of the reticulo-endothelial system in the peripheric blood.
In 1960-1962 the authors conducted studies, namely laboratory and gynecological investigations of 591 women living in three rural settlements of the Lublin province. The aim of these investigations was to determine the percentage of Trichomonas vaginalis infections in the country women and an evaluation of the adapted diagnostic methods (direct preparations, stained preparations according to Gram and Giemza, Schneider's medium and Pavlova's medium). Among the 591 women supposed to be healthy only 19 of them were free from the complaints. The authors found that inflammations of the genital organs and post-parturition injuries were the most common diseases. The rural settlement Turobin showed 19 per cent of Trichomonas vaginalis infection, Józefów - 8,1 per cent and Rudnik - 20,4 per cent. Comparative investigations of the evaluation of the used methods for the diagnosis showed that the best methods are: the method of direct preparation and Schneider's medium. This medium secured the highest increase of the number of protozoons and the longest durability. According to the authors such investigations should be initiated in other provinces of Poland.
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