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Lupine diseases caused by pathogenic fungi constitute a serious problem in agriculture. They lead to partial yield loss and deterioration of crop quality through the changes in biochemical composition of seeds or their contamination with mycotoxins. Some of common lupine diseases are fusarioses caused by Fusarium oxyspomm f. sp. lupini. Morphometric and metabolic changes were investigated in yellow lupine seedlings infected with F. oxyspomm f. sp. lupini. It was found that infection caused temporary inhibition of seedling growth, overcome at later development, and activation of ß-glycosidase and peroxidases. The changes in enzymes activity indicate the induction of defense mechanism against F. oxyspomm f. sp. lupini and inhibition of pathogen spread.
In most fungal pathogen–plant systems, a high level of sugars in plant tissues enhances plant resistance. Several hypotheses have been proposed to explain the mechanisms of ‘‘high-sugar resistance’’. Sugars constitute the primary substrate providing energy and structural material for defense responses in plants, while they may also act as signal molecules interacting with the hormonal signaling network regulating the plant immune system. Sugars enhance oxidative burst at early stages of infection, increasing lignification of cell walls, stimulate the synthesis of flavonoids and induce certain PR proteins. Some sugars act as priming agents inducing higher plant resistance to pathogens.
The ever-increasing environmental pollution necessitates organisms to develop specific defense systems in order to survive and function effectively. Lead is taken up by plants mainly through roots and over 96% are accumulated there.Pea plants were cultivated hydroponically for 4 days with 0.1, 0.5 and 1 mM Pb(NO3)2. Uptake of lead ions from nutrient solution and accumulation in root stems and leaves during 96-h cultivation was estimated. The root tip cells were observed with transmission electron microscope to analyse their ultrastructure and lead localization. Pb was accumulated in the cell wall, cell membrane, vacuoles, mitochondria and peroxisomes. The fractions of mitochondria and peroxisomes were isolated from pea roots purified by means Percoll gradient, and were observed by means of electron microscope with the attachment for X-ray microanalysis. Visible deposits containing Pb were observed in both cell organelles.
Aphid feeding induces various defense signaling mechanisms in plants. The recognition of feeding activities by plants occurs through the use of transmembrane pattern recognition receptors (PRRS) or, acting largely inside the cell, polymorphic nucleotide-binding leucine-rich-repeat (NB-LRR) protein products, encoded by most R genes. Activation may induce defensive reactions which are the result of highly coordinated sequential changes at the cellular level comprising, among other changes, the synthesis of signaling molecules. The ensuing plant responses are followed by the transmission of defense response signal cascades. Signals are mediated by bioactive endogenous molecules, i.e. phytohormones, such as jasmonic acid (JA), salicylic acid (SA), ethylene (ET), abscisic acid (ABA), gibberellic acid (GA) and free radicals such as hydrogen peroxide (H₂O₂) and nitric oxide (NO) which independently provide direct chemical resistance. Plant-induced defenses are also regulated by a network of inter-connecting signaling pathways, in which JA, SA, and ET play dominant roles. Both synergistic and inhibitory aspects of the cross-talk among these pathways have been reported. This paper presents molecular mechanisms of plant response to aphid feeding, the precise activation of various endogenous bioactive molecules signaling in the response of many plant species and their participation in the regulation of numerous defense genes, which lead to a specific metabolic effect. Selected important points in signal transduction pathways were also discussed in studies on plant response to aphid feeding.
The aim of the studies was to examine whether carbohydrates being an important nutritious agent and releasing a metabolic signal may play an essential role in plant resistance to fungal infections. At the early stages of seed germination in legume plants there occurs an intensive carbohydrate consumption for respiratory processes. A significant loss of carbohydrates in the germinating seed axis leads to the so-called carbohydrate starvation, which lasts up to the moment before the developing seedling starts independent sugar synthesis. Contemporary breeding cultivars of yellow lupin are especially subjected to carbohydrate starvation, because their selection conducted with the aim to increase protein content, has consequently caused a simultaneous drop in soluble sugars in seeds. In the earliest period of development, unfavourable environmental conditions as well as deep sowing are conducive to the occurrence of fungal infections. The most common fungal diseases of seedlings are fusarioses, the first symptons of which are visible already at the time of plant emergence. So, it seems that the level of carbohydrates in seeds may play an important role in plant defense against diseases. Assuming that plants have developed a wide range of constitutive and induced defense mechanisms preventing or restricting pathogen invasion, the purpose of the present studies was to determine whether the level of carbohydrates influences plant response at the moment of pathogenic fungal attack. Since it is known that one of the first plant cell responses to pathogen invasion is the so- called oxidative burst generating free radicals, the present paper contains results of determinations of the level of free radicals and Mn⁺² ions in yellow lupin embryo axes infected with Fusarium oxyspomm f. sp. lupini. The experiments were performed on the embryo axes of yellow lupin, which after the infection were cultured in vitro for 24-96 hours on medium with 60 mmol・dm⁻³ sucrose and without sugar. Results of free radical measurements made by EPR method have showed the presence of free radicals the level of which was higher in the embryo axes infected with the spores of Fusarium oxysporum f. sp. lupini than in the noninfected material. Comparing also the free radical concentration in the material infected with Fusarium oxyspomm f. sp. lupini cultured under different trophic conditions, it has been found that the free radical level in the lupin embryo axes increased at the lack of carbohydrates. Lupin embryo axes cultured on medium with sucrose have been found to have an increase of Mn⁺² ions concentration under the influence of infection, particularly after 76 and 96 hours of culture. The obtained results suggest that the resistance mechanism of sugar-fed tissues consists in levelling free radical toxic effect.
Soluble carbohydrates, particularly oligosaccharides, can take part in defense responses preventing and restricting fungal pathogen invasion. Morphological changes were observed and β-glucosidase activity was studied in yellow lupin embryo-axes infected and uninfected with F. oxysporum and cultured for 24 lto 96 hours under conditions of a varying carbohydrate supply. The first disease, symptoms - necrotic pathological changes were observed 48 hours after the .inoculation. A higher intensity of the disease changes as well as growth restriction Cle found in 72- and 96-h embryo axes growing under carbohydrate deficiency. The activity of ậ-glucosidase increased during 24 - 96 hours after inoculation. An especially high increase of the activity was noted in 72- and 96-h embryo axes cultured under carbohydrate deficiency. We suggest that sugars are involved in mechanisms of yellow lupin embryo axis tissues resistance, since they constitute source of precursors for the synthesis of antimicrobial factors of plant defense response.
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