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Eyespot belongs to most destructive of foot rot diseases of cereals, because it occurs on the best soils and owing to a great number of host plants. To assure a satisfactory chemical protection of cereals against Pseudocercosporella herpoirichoides (Fron) Delghton the knowledge of the etiology of this disease and the biology of the pathogenic agent is needed. Till now the benzimidazole fungicides are used on a large scale. Since 1982 in West Europe the resistance of the fungus - P.herpotrichoides to benzimidazole fungicides has been observed. Thus the necessity of exact investigation of this problem in agroecological conditions of Poland is important.
The activity of picoxystrobin and its mixtures with standard fungicides against isolates of eyespot cereal disease agents was estimated in laboratory in vitro tests. The tested Ramulispora herpotrichoides and R. acuformis isolates differed in response to carbendazim. Picoxystrobin at concentration from 0.05 to 50 mg/L not totally inhibited the mycelium growth of tested pathogens on PDA medium. But carbendazim sensitive R. herpotrichoides isolates were a little more susceptible to strobilurin derivate than carbendazim resistant R. herpotrichoides isolate and R. acuformis strains. The effect of picoxystrobin at concentration 0.5 mg/L as partner in mixture with standard fungicide at concentration 0.1 mg/L (carbendazim, prochloraz or cyprodinil) was assessed as the additive. It was relatively most noticeably in tests with R. acuformis and picoxystrobin plus cyprodinil mixture that was for mixture recommended as tank-mix or as manufacture product.
Prom winter wheat and winter triticale stems with distinct disease lesions 23 isolates of eyespot agent - Tapesia yallundae fungus were obtained. Stems ere collected in 1999-2001, from no-treatment and protected with fungicides fields, which were situated in three provinces of Poland (małopolskie, mazowieckie, śląskie). Obtained isolates were c1assified on the basis of morphology on PDA, sporulation on water agar and sensitivity to carbendazim and prochloraz. 51 isolates 41,5% of a11) represent R- growth type of fungus. To this type belong 28% 71% strains obtained from wheat and triticale stems, respectively. Only seven isolates of R-type fungus (i.e. 13,7%) were resistant to carbendazim. These strains originated from two protected with carbendazim winter wheat field s and two, rotected winter triticale crops. All isolates of R-type were sensitive to prochloraz. This compounds appears in vitro more effective than flusilazole, propiconazole and azoxystrobin.
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