Wyniki wyszukiwania

1

The role of focal adhesion kinase [FAK] in contact inhibition

100%

Miloszewska J., Trembacz H., Malecki M., Janik P.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 2

2

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

100%

Miloszewska J., Trembacz H., Janik P.

Acta Biochimica Polonica

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2001

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tom 48

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nr 1

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarithmic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK upstream elements we studied the integrin receptor gene transcript by RT-PCR and focal adhesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.

3

The role of FNIII/10 and FNIII/10 RGD fibronectin ragments in contant inhibition of C3H10T1/2 cells

88%

Miloszewska J., Gos M., Trembacz H., Przybyszewska M., Janik P.

Acta Biochimica Polonica

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2003

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tom 50

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nr Supplement 1

4

Cancer stem cells from mouse sarcoma cell line- L1: preliminary results

88%

Miloszewska J., Gos M., Przybyszewska M., Szaniawska B., Trembacz H.

Acta Biochimica Polonica

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2006

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tom 53

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nr Supplement 1

5

Metastatic capacity stimulated by cancer cells cultivation in non-adhesive conditions

88%

Gos M., Miloszewska J., Trembacz H., Przybyszewska M., Janik P.

Acta Biochimica Polonica

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2006

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tom 53

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nr Supplement 1

6

The role of focal adhesion kinase in the emigration of cells from confluent cultures

76%

Trembacz H., Miloszewska J., Szaniawska B., Malecki M., Przybyszewska M., Skorski T., Janik P.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.

7

Does VEGF165 and sFLT gene transfer prevent the radiation-induced lung fibrosis?

76%

Pszybyszewska M., Miloszewska J.-J., Rzonca S., Pysniak K., Malecki M., Trembacz H.

Acta Biochimica Polonica

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2007

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tom 54

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nr Supplement 4

8

Soluble TNF-alpha receptor 1 encoded on plasmid vector and its application in experimental gene therapy of radiation-induced lung fibrosis

64%

Przybyszewska M., Miloszewska J., Rzonca S., Trembacz H., Pysniak K., Kotlarz A., Swoboda P., Zalewska M., Malecki M.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 4

9

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Different mutations in Polish patients with HPRT deficiency - the Lesch-Nyhan and Kelley-Seegmiller syndromes

64%

Popowska E., Sulek A., Kubalska J., Pronicka E., Jezewska M., Trembacz H., Goryluk-Kozakiewicz B., Krajewska-Walasek M.

Journal of Applied Genetics

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1998

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tom 39

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nr 1

Five families with the Lesch-Nyhan syndrome (LNS) and two families with the Kelley-Seegmiller syndrome (KSS) were studied. Seven different mutations were identified. Two transitions, C526→ T (Prol76Ser) and G481→A (Ala161Thr), in patients with a milder form of hypoxanthine-guanine phosphoribo-syltransferase (HPRT) deficiency were detected. In patients with the Lesch-Nyhan syndrome two transitions, G569→A (Glyl90Glu) and C508→T (Arg170Ter), two transversions, C222→A (Phe74Leu) and C482→A (Ala161Glu), and a deletion of seven nucleotides (from A394 to G400) were observed. All except two of the identified mutations are novel. The C222→A substitution in exon III is located within one of the clusters of hot spots of the HPRT gene and has been previously described in four unrelated patients. The other recurrent mutation C508→T in exon VII has been reported in eight families.

Ograniczanie wyników

The role of focal adhesion kinase [FAK] in contact inhibition

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

The role of FNIII/10 and FNIII/10 RGD fibronectin ragments in contant inhibition of C3H10T1/2 cells

Cancer stem cells from mouse sarcoma cell line- L1: preliminary results

Metastatic capacity stimulated by cancer cells cultivation in non-adhesive conditions

The role of focal adhesion kinase in the emigration of cells from confluent cultures

Does VEGF165 and sFLT gene transfer prevent the radiation-induced lung fibrosis?

Soluble TNF-alpha receptor 1 encoded on plasmid vector and its application in experimental gene therapy of radiation-induced lung fibrosis

Different mutations in Polish patients with HPRT deficiency - the Lesch-Nyhan and Kelley-Seegmiller syndromes