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1

Multivariate analysis and heavy metals pollution evaluation in Yellow River surface sediments

100%
Ren J., Shang Z., Tao L., Wang X.
Polish Journal of Environmental Studies
|
2015
|
tom 24
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nr 3
2
Content available remote

Significant, but limited collateral blood flow increases occur with prolonged training in rats with femoral artery occlusion

100%
Prior B. M., Ren J., Terjung R. L., Yang H. T.
Journal of Physiology and Pharmacology
|
2011
|
tom 62
|
nr 2
This study examined the capacity of collateral dependent blood flow induced by a prolonged treadmill training program, as compared to a low collateral resistance model created by femoral artery to vein (A-V) shunt. Sprague-Dawley rats, with bilateral femoral artery occlusion were confined to cage activity (Sed, n=9) or trained by daily treadmill exercise (Tr, n=15; up to ~350 min/d) for 15 weeks. Another set of animals received a femoral A-V anastomosis in one limb and treated with (n=4) or without VEGF165 (n=9) infusion for 2 weeks. The contralateral side was used as control. Blood flow (BF) was measured with isotope labeled microspheres. Maximal calf muscle BF increased by 15 week training (up to 100±5.0 ml·min-1·100g-1 (p<0.05); 0.71±0.04 ml·min-1·100g-1·mmHg-1), a response better (20-25%) than the less demanding training programs used previously. In contrast, femoral A-V shunt with VEGF165 increased calf muscle conductance to 1.70±0.3 ml·min-1·100 g-1·mmHg-1 that is similar to blood flows observed in non-occluded rats during maximal running. Our data indicate that the collateral circuit development is related to the driving stimulus and that exercise training, does not provide a maximal stimulus for adaptation that is possible. Nonetheless, exercise training results in profound increases in exercise capacity associated with this enhanced collateral blood flow. Our results illustrate that vascular adaptations can be much greater when physiologically induced stimuli are enhanced at the time of therapeutic angiogenesis.
3

Prediction of ship motions via a three-dimensional time-domain method following a quad-tree adaptive mesh technique

100%
Zhang T., Ren J., Liu L.
Polish Maritime Research
|
2020
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tom 27
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nr 1
A three-dimensional (3D) time-domain method is developed to predict ship motions in waves. To evaluate the FroudeKrylov (F-K) forces and hydrostatic forces under the instantaneous incident wave profile, an adaptive mesh technique based on a quad-tree subdivision is adopted to generate instantaneous wet meshes for ship. For quadrilateral panels under both mean free surface and instantaneous incident wave profiles, Froude-Krylov forces and hydrostatic forces are computed by analytical exact pressure integration expressions, allowing for considerably coarse meshes without loss of accuracy. And for quadrilateral panels interacting with the wave profile, F-K and hydrostatic forces are evaluated following a quad-tree subdivision. The transient free surface Green function (TFSGF) is essential to evaluate radiation and diffraction forces based on linear theory. To reduce the numerical error due to unclear partition, a precise integration method is applied to solve the TFSGF in the partition computation time domain. Computations are carried out for a Wigley hull form and S175 container ship, and the results show good agreement with both experimental results and published results
4
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Factors affecting asparagus (Asparagus officinalis L.) root development in vitro

100%
Ren J., Chen W., Knaflewski M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2012
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tom 11
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nr 6
Parental lines are propagated in vitro in asparagus breeding. Additionally, in order to obtain super males which are needed for breeding all-male hybrids, anther culture is applied. Healthy and viable asparagus parental plants developed from in vitro culture should contain well formed storage roots. There are many factors affecting asparagus rooting. Only with the healthy explant materials, proper culture medium and growth conditions can asparagus plantlets be produced and later successfully grown in the greenhouse. In this article, obstacles and factors affecting asparagus rooting in vitro as well as trends in developing rooting technique are summarized.
5

Effects of cadmium on seed germination, coleoptile growth, and root elongation of six pulses

100%
Tao L., Guo M., Ren J.
Polish Journal of Environmental Studies
|
2015
|
tom 24
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nr 1
The ecotoxicological effects of Cd²⁺ on germination and early seedling growth of six pulses were investigated. Seeds of these plants were exposed to seven different concentrations of Cd (0, 0.1, 0.2, 0.4, 0.8, 1.6, and 3.2 mM). The results indicated that root and coleoptile growth of six pulse plants were more sensitive than seed germination for measurement of toxic Cd²⁺ pollution. Different species show different levels of tolerance to Cd²⁺ pollution. V. angularis and Dumasia villosa are the most sensitive to Cd²⁺, and their germination percentage, and root and coleoptile growth were significantly lower than other tested species. By contrast, Vigna radiata and Lablab purpureus are the most resistant species, their germination and seedling growth almost were not influenced by Cd²⁺ pollution significantly compared to control. There were significantly negative correlations between seedling growth and increasing concentrations of Cd²⁺ for V. angularis. V. angularis and Dumasia villosa are the most sensitive to Cd²⁺. By contrast, Vigna radiata and Lablab purpureus are the most resistant species.
6

Pb removal using mixed substrates in a constructed laboratory-scale unvegetated vertical subsurface-flow wetland

100%
Ren J., Gao S., Tao L., Li H.
Polish Journal of Environmental Studies
|
2016
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tom 25
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nr 1
In this study, five mixed substrates – SSFGF, FSSGF, FSSFF, FSSFG, and FSFGF – consisting of fly ash, sludge, soil, fine cinder, gravels, and fine sand, were made in order to test the removal effect of lead. Multiple comparisons and analysis of variance were used to analyze physicochemical properties and the removal efficiency of lead. The test results showed that for five substrates, the effluent concentrations were not significantly different among 10 mg/L and 40 mg/L initial concentrations. For the other three initial concentrations (20 mg/L, 80 mg/L and 160 mg/L) from five substrates, it was significantly different. Moreover, when decomposition time was 0.25 h~48 h, the removal efficiency of Pb from the solution was better for substrate SSFGF-based wetland than those of the other substrate-based wetlands at low and medium initial concentrations (10 mg/L, 20 mg/L, 40 mg/L, and 80 mg/L), and then it was slightly better for substrate FSSFF-based wetland. At relatively high initial concentrations (160 mg/L), the removal efficiency was better for substrate FSSFF-based wetland than those of the other substrate-based wetlands, and then it was slightly better for substrate SSGFF-based wetland. Our study further suggested that SSFGF and FSSFF were the two relatively ideal substrate materials suitable for removal of Pb from a constructed wetland system.
7

Sex-specific physiological and growth responses to water stress in Hippophae rhamnoides L. populations

100%
Li C., Ren J., Luo J., Lu R.
Acta Physiologiae Plantarum
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2004
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tom 26
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nr 2
8

Effect of sludge biochars obtained at different pyrolysis temperatures on the adsorption of Cd(II)by loess in northwestern China

88%
Dai L., Ren J., Tao L., Li H., Hao J. X.
Polish Journal of Environmental Studies
|
2017
|
tom 26
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nr 4
The effect of sludge biochars obtained at different temperatures on the adsorption of Cd(II) by loess in northwestern China was investigated using the batch equilibrium method. The results showed that the pseudo second-order kinetic model can describe the kinetics of Cd(II) adsorption on the sludge biocharamended loess very well. The adsorption of Cd(II) by loess reached equilibrium in 6h. However, with the addition of biochar, the adsorption time of the Cd(II) by loess decreased. In addition, the adsorption equilibrium time decreased significantly with increasing pyrolysis temperatures. When the pH increased from 2 to 7, the adsorption capacity of biochar-amended loess increased, while the adsorption capacity was in equilibrium or even slightly decreased in the pH range from 7 to 8. The Langmuir model can describe the adsorption isothermal behaviours of Cd(II) by biochar-amended loess. The adsorption capacity of biochar-amended loess samples for Cd(II) at 25°C follows the order of LSB700 (28.74 mg/g)>LSB500 (22.42 mg/g)>LSB300 (17.67 mg/g). The addition of sludge biochars can significantly improve the saturation adsorption capacity of loess for Cd(II). Therefore, the addition of sludge biochars can effectively improve the adsorption-immobilization capability of loess for Cd(II).
9

Specific role of the tight junction proteins occludin and claudin-5 on the blood–brain barrier during Listeria monocytogenes infection

88%
Ren J., Yang M., Wang P., Jiang J., Yan G.
Medycyna Weterynaryjna
|
2020
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tom 76
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nr 06
To investigate the blood-brain barrier (BBB) permeability of mice after Listeria monocytogenes infection for further study on the mechanism of L. monocytogenes crossing the BBB, a mouse model was established and Evans blue assay was performed to assess the BBB disruption. Using relative quantitative real-time PCR, the RNA expression of Zonula occludens-1 (ZO-1), occludin and claudin-5 were detected. In addition, the protein expression level of ZO-1, occludin and claudin-5 were detected by immunohistochemistry and western blot. The extravasation of Evans blue dye was significantly different between 24 h and 96 h (P < 0.05). The mRNA expression of occludin and claudin-5 were down-regulated than that of the control group at each sampling point (P < 0.05) and ZO-1 showed a significant change at 96 h (P < 0.05). In addition, the protein expression level of occludin and claudin-5 decreased significantly at 48 h and 96 h (P < 0.05) by immunohistochemistry and western blot, compared with the control, while ZO-1 was almost unchanged (P > 0.05). All results indicating that the tight junction integrity of endothelial cells was destroyed and BBB permeability was enhanced in the process of L. monocytogenes infection, and this change was related to the decrease of the expression occludin and claudin-5.
10

Quantitative research on the relationship between salinity and crack length of soda saline-alkali soil

88%
Ren J., Li X., Zhao K., Zheng X., Jiang T.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 2
The Songnen Plain in China has a typical type of soda saline-alkali soil that frequently shrinks and cracks under natural conditions, especially during water evaporation. This study aims to study the effects of salt contents on the cracking process of soda saline-alkali soils and to make a quantitative analysis between crack length and some primary salt parameters of soda saline-alkali soils. To achieve the objectives, 57 soil samples with different salinity levels were selected and a laboratory-controlled desiccation cracking test was then conducted. Next, correlation analysis was carried out between extracted crack lengths and salt parameters of all the specimens. The results showed that there are clear linear dependencies between crack length and salinity, Na⁺, Cl⁻, and electrical conductivity (EC), and that CO₃²⁻ is also quite linear with crack length. The fitting results conducted from the verification experiment showed that Na⁺ content, Cl⁻ content, EC, and total salinity showed high prediction performance (R²>0.91 and RPD>2.5); that the CO₃²⁻ content had quite reasonable accuracy (R²= 0.78 and RPD = 1.82) but with less stability and poor reliability; that the prediction accuracy for pH, however, was very poor, with R² and RPD only 0.42 and 1.06, respectively.
11

Crosstalk between ABA, auxin, MAPK signaling, and the cell cycle in cadmium-stressed rice seedlings

76%
Zhao F. Y., Wang K., Zhang S. Y., Ren J., Liu T., Wang X.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 07
We studied the link between abscisic acid (ABA), auxin, MAPK (mitogen-activated protein kinase) signaling, and the cell cycle in cadmium (Cd)-stressed rice (Oryza sativa L. cv. Zhonghua No. 11) roots. ABA can partially compensate for root growth inhibition and counteract over-accumulation of auxin caused by Cd. GUS staining of rice plants harboring DR5-GUS showed that ABA affects auxin distribution in Cd-stressed roots. Detection using DRB (5,6-dichlorobenzimidazole 1-β-ᴅ-ribofuranoside, an RNA synthesis inhibitor), MG132 (a protein degradation inhibitor), BFA (brefeldin A, a protein transport inhibitor), and TIBA (2,3,5-triiodobenzoic acid, a polar auxin transport inhibitor) revealed that ABA regulates the distribution of auxin via transcription, protein degradation, and transport pathways under Cd stress. Several genes related to ABA and MAPK, key components of the auxin signaling pathway, and the cell cycle were differentially regulated by Cd plus ABA vs. Cd plus tungstate (TS) (a ABA biosynthesis inhibitor) at 7 days or 11 days of treatment in roots, indicating that ABA levels affect the transcription of these genes, and that these genes are differentially regulated by ABA in rice seedlings at different developmental stages. Furthermore, the expression of some of these genes differed between Cd + ABA/TS-treated plants and plants treated with TS or ABA alone, suggesting that ABA signaling serves specific functions in the regulation of gene expression under Cd stress. Overall, these results suggest that ABA coordinates auxin and MAPK signaling and the cell cycle in response to Cd stress. The ABA signal transduction pathways in Cd-stressed rice plants are discussed.
12

A comparison of Su and Lu modeling of hydro-thermal coupling model using field temperature records

76%
Ren J., Zhang W., Chen B., Men L., Ma C., Gan L.
Polish Journal of Environmental Studies
|
2021
|
tom 30
|
nr 1
13

Physiological characterization and transcriptome analysis of a chlorosis mutant in pak choi

76%
Ye X., Ren J., Huang S., Chi M., Zhang Y., Feng H., Liu Z.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 07
Leaf color mutants are not only ideal resources for studying the physiological mechanisms of plant photosynthesis, but they are also selection markers for hybrid production. In this study, the chlorosis mutant ‘564Y’ and its wild-type counterpart ‘564’ were used to explore physiological characteristics and gene expression in pak choi. Compared with those of wild type, the dry weight and fresh weight of ‘564Y’ were significantly reduced, but the hypocotyl length of ‘564Y’ was longer. Pigment analysis showed that the chlorophyll a and carotenoid contents of ‘564Y’ were lower than those of wild type. However, there was no significant difference in chlorophyll b content between these two varieties. The photosynthetic capacity of ‘564Y’ was lower than that of ‘564’, and the photosynthetic electron transfer ability and light energy conversion efficiency were weaker in the former. Transmission electron microscopy showed that the chloroplast structure of ‘564Y’ was not compact, the thylakoid structure was irregular, and the number of matrix, grana, and stacking layers was decreased. Transcriptome analysis revealed 2958 differentially expressed genes (DEGs), and 78 gene ontology terms and 35 Kyoto Encyclopedia of Genes and Genomes pathways were significantly enriched. Among the DEGs, nine genes were associated with the chlorophyll synthesis pathway (Bra031690, Bra012595, Bra005677, Bra022628, Bra026410, Bra032155, Bra039206, Bra029875, and Bra012511) and one gene was associated with photosynthesis (Bra038011). Among these genes, 3 were identified as related to auxin metabolism too (Bra032155, Bra039206, and Bra029875). Their differential expression in the ‘564Y’ mutant and wild-type plant was confirmed by qRT-PCR. These results lay the foundation for future genetic and functional genomic studies in pak choi.
14

Isolation and molecular characterization of the porcine SLC6A14 gene excludes it as a candidate gene for fat deposition and growth

64%
Yang G. L., Ren J., Zhang S. H., Huang W. B., Guo Y. M., Duan Y. Y., Liu M. Z., Huang L. S.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 3
The gene encoding solute carrier family 6 member 14 (SLC6A14) has been considered as a candidate gene affecting human obesity. In this study, full-length cDNA (2237 bp) and DNA sequence (24 541 bp) of the porcine SLC6A14 gene were isolated. The porcine SLC6A14 cDNA contains a 5'-untranslated region of 57 bp, a 3' -untranslated region of 254 bp, and an open reading frame of 1926 bp, encoding a deduced protein of 642 amino acids with a molecular mass of 72. 475 kDa and an isoelectric point of 7.82. The genomic structure of the porcine SLC6A14 gene is similar to mammalian orthologs, particularly in terms of exon size and exon/intron boundaries. It comprises 14 exons and 13 introns. A semi-quantitative RT-PCR showed that the porcine SLC6A14 mRNA expression was tissue-specific. Four SLC6A14 single-nucleotide polymorphisms (SNPs) were identified, and 3 informative SNPs were chosen for genotvping in a White Duroc × Erhualian resource population with phenotvpe data of growth and fatness traits. The association analysis showed that the c. 1438 G>A nonsynonymous polymorphism was associated with birth weight and 21-day body weight (P < 0.05), while g.7944 A>T was associated with 46-day body weight. Linkage and radiation hybrid mapping assigned SLC6A14 to a region around SW1522 on SSCXp13, which did not fall in the confidence interval of the quantitative trait locus (QTL) for growth and fatness traits on SSCX in the resource population. These results indicate that SLC6A14 is not a positional candidate gene for the QTL affecting fatness and growth traits in pigs.
15

VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance

64%
Dong C., Zhang Z., Qin Y., Ren J., Huang J., Wang B., Lu H., Cai B., Tao J.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 10
To understand cold acclimation and cold tolerance in Vitis, we isolated a C-repeat binding factor 1 (CBF1) transcriptional activator from cold-sensitive Vitis vinifera ‘Manicure Finger’ and cold-tolerant wild Vitis amurensis. Under cold stress, the CBF1 transcript accumulation of V. amurensis increased, whereas that of V. vinifera showed no significant change. The transcript levels of VaCBF1 in the roots, stems, leaves, and petioles under cold stress were up-regulated in a time-dependent manner. The transcript level of VaCBF1 in the leaves was induced by salinity stress or by exogenous abscisic acid and salicylic acid. The presence of the cis-elements MBS, MYB, and MYC in the VaCBF1 promoter suggests that this promoter is a component of the CBF transduction pathway, which is involved in plant response to cold stress. The overexpression of VaCBF1 increased the cold tolerance of transgenic tobacco at −4 °C. The transcript level of the downstream target gene NtERD10D appeared in the transgenic lines under normal conditions, whereas that of NtERD10D and NtDREB3 improved under low temperature. We suggest that VaCBF1 enhances stress tolerance by increasing antioxidant activities and promoting downstream target gene expression.
16

A genome-wide association study identifies genomic loci associated with backfat thickness, carcass weight, and body weight in two commercial pig populations

64%
Guo Y., Qui H., Xiao S., Wu Z., Yang M., Yang J., Ren J., Huang L.
Journal of Applied Genetics
|
2017
|
tom 58
|
nr 4
17

Transcriptional profile of the spring freeze response in the leaves of bread wheat (Triticum aestivum L.)

64%
Kang G., Li G., Yang W., Han Q., Ma H., Wang Y., Ren J., Zhu Y., Guo T.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 02
Measurement of the electrolyte leakage rates in wheat leaves indicated that there was no significant difference in susceptibility to -5 C spring freeze stress among five bread wheat cultivars at the floret primordiumdifferentiating stage of spike development. A global transcriptional profile was created using the Affymetrix Wheat GeneChip microarray for one wheat cultivar (Yumai 34) under -5 C freeze stress. After assaying genes with significant regulation at 1 and 3 days after -5 C freeze stress, we identified 600 genes that were previously annotated as showing changes in expression of at least than two-fold at one or both of the time points. Among these genes, we further analysed 102 genes whose expression levels changed at least eight-fold after 1 or 3 days of freeze stress. These genes encoded an ice recrystallization protein, cold-related proteins, CBF transcription factors, calcium-dependent protein kinases, Na?/H? antiporters, aquaporins, and many metabolic enzymes. The results of this study were compared with those of a previous study on the sub-freeze hardening response in wheat and spring freeze stress in wheat and barley. Many genes, including those encoding WCOR413, LEA, glycine-rich RNA-binding protein, ferritin, aquaporin 2, and a pathogen-induced protein, showed similar expression levels in these studies. Spring freeze stress is a complex phenomenon involving physiological mechanisms and multiple genes that had not been previously characterised.
18

IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes

64%
Xiong W., Chen H., Lu J., Ren J., Nie C., Liang R., Liu F., Huang B., Luo Y.
Folia Histochemica et Cytobiologica
|
2021
|
tom 59
|
nr 3
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