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The present review deals with the bioactive compounds of the marine non-chordates. The potent medicinal usage of the bioactive compounds viz. steroids, terpenoids, isoprenoid and non-isoprenoid compounds, quinones, brominated compounds, nitrogen heterocyclics and nitrogen-sulphur heterocyclics from marine non-chordates have been compiled. Various literatures survey revealed that the bioactive compounds isolated in recent past from the marine poriferans, cnidarians, annelids, arthropods, molluscs and echinoderms could be rich sources of therapeutic agents having antibacterial, antiinflamatory, anticarcinogenic properties. In overall, the present study will be benefitted to know global drug discovery researches on bioactive compounds from marine organisms for students, scholars, scientists, pharmaceutical sector, and government regulating authorities as new challenging technology in clinical applications through medicines.
The present study aims to detect morphological abnormalities in Zygnema sp. because this test species have high adaptive capacity in unfavourable environmental stress. This alga is inhabited at the suburban bank of river Ganga, West Bengal, India. The study was done at experimental sites, downstream at Khardah near sewage water mixing area was compared with upstream control site Kalyani as no sewage water discharge. External and internal abnormal morphology such as undulating or wavy and extra outgrowth on cell wall, sometimes bending inwardly and outwardly as external deformities while internal anomalies such as pigment loss, nucleus was in circular, rectangular, oval and amoeboid shapes, also vacuolated and trilobed, broom like chloroplasts and central part of the thallas dried in between two segments. It was observed that this test species was surviving and well adapted with above mentioned deformities. The present preliminary study with Zygnema sp. can be utilized for in situ and ex situ indicator species for riverine biomonitoring. Further research may be relevant to know both tolerant and sensitive algae species inhabited at the river Ganga bank alongwith biochemical and genetic parameters. The present work is suggesting that other inhabiting algae and this adaptive test species can be used for bioremediation of pollutants.
Many information of biological study as stained cells analysis under microscope cannot be obtained rich information like detail morphology, shape, size, proper intensity etc. but image analysis software can easily be detected all these parameters within short duration. The cells types can be yeast cells to mammalian cells. An attempt has been made to detect cellular abnormalities from an image of metronidazole (MTZ) treated compared to control images of peripheral erythrocytes of fish by using non-commercial, open-source, CellProfiler (CP) image analysis software (Ver. 2.1.0). The comparative results were obtained after analysis the software. In conclusion, this image based screening of Giemsa stained fish erythrocytes can be a suitable tool in biological research for primary toxicity prediction at DNA level alongwith cellular phenotypes. Moreover, still suggestions are needed in relation to accuracy of present analysis for Giemsa stained fish erythrocytes because previous works have been carried out images of cells with fluorescence dye.
The trees support many bird and animal species while the survival of urban-dwelling species e.g. bird species dominate over others. This particular Nature park in Kolkata is the habitat of birds and fish species suggesting a better environment for food chain maintenance. The Asian open bill stork, Anastomus oscitans among other bird species is a common and provides ecosystem health indicator. The present study aims to understand the occurrence and population nos. of Asian open bill stork on particular tree species surrounding wetlands at Nature Park, Kolkata, India. Our results clearly indicate that a total population of this bird species having 671 nos. on the particular trees but not in all other available trees in the study area. In conclusion, present research emphasizes a novel occurrence and higher population of the A. oscitans being observed on the basis of the presence of particular tree species, though no attempt has been made on physico-chemical properties of present water and microclimate within the park. Therefore, further research planning should be oriented considering the physico-chemical impacts as well as local microenvironment along with human interaction as visitors in such a way so that these barriers cannot affect nesting and breeding of those species as eco-indicator. Also further relevant research is needed to emphasize on the conservation of these specific tree species to increase population of Asian open bill stork, A. oscitans in Nature park.
Enterococcus gallinarum strains isolated from some Nigerian fermented foods were found to produce bacteriocins. The bacteriocins had a broad spectrum of activity against both Gram-positive and negative bacteria. The effects of the bacteriocins and bacteriocinogenic organisms on Staphylococcus aureus infections in rats were evaluated. Sprague-Dawley rats were infected with S. aureus MTCC 737 and treated with E. gallinarum T71 and different concentrations of the bacteriocins from E. gallinarum W211 and T71. Staphylococcus aureus infection caused significant upregulation of aspartate aminotransferase and alanine aminotransferase levels in sera of the infected rats. Moreover, gelatin zymography revealed that infected gastric tissues showed elevated matrix metalloproteinase-9 activity. Bacteriocin treatments reduced the MMP-9 activity and inhibited the expressions of both Tumour Necrosis Factor Alpha (TNF-α) and Interleukin-1 Beta (IL-1β) dose dependently, pointing to a potential role of the bacteriocins in attenuating inflammatory responses to Staphylococcus aureus infection. Gastric and GIT damage caused by staphylococcal infection were reduced in the Enterococcus gallinarum T71 and bacteriocin-treated groups also dose dependently. We conclude that these bacteriocins may have useful biomedical applications.
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