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Further evidence for cryptic north-western refugia in Europe? Mitochondrial phylogeography of the sibling species Pipistrellus pipistrellus and Pipistrellus pygmaeus

100%

Boston E. S. M., Puechmaille S. J., Clissmann F., Teeling E. C.

Acta Chiropterologica

2014

tom 16

nr 2

The geographic ranges of European plants and animals underwent periods of contraction and re-colonisation during the climatic oscillations of the Pleistocene. The southern Mediterranean peninsulas (Iberian, Italian and Balkan) have been considered the most likely refugia for temperate/warm adapted species. Recent studies however have revealed the existence of extra-Mediterranean refugia, including the existence of cryptic north-west European refugia during the Last Glacial Maxima (24–14.6 kyr BP). In this study we elucidated the phylogeographic history of two sibling bat species, Pipistrellus pipistrellus and P. pygmaeus in their western European range. We sequenced the highly variable mtDNA D-loop for 167 samples of P. pipistrellus (n = 99) and P. pygmaeus (n = 68) and combined our data with published sequences from 331 individuals. Using phylogenetic methodologies we assessed their biogeographic history. Our data support a single eastern European origin for populations of P. pygmaeus s.str., yet multiple splits and origins for populations of P. pipistrellus s.str., including evidence for refugia within refugia and potential cryptic refugia in north western Europe and in the Caucasus. This complex pattern in the distribution of mtDNA haplotypes supports a long history for P. pipistrellus s.str. in Europe, and the hypothesis that species with a broad ecological niche may have adapted and survived outside southern peninsula throughout the LGM.

The status of the cryptic bat species, Myotis mystacinus and Myotis brandtii in Ireland

64%

Boston E. S. M., Buckley D. J., Bekaert M., Gager Y., Lundy M. G., Scott D. D., Prodohl P. A., Montgomery W. I., Marnell F., Teeling E. C.

Acta Chiropterologica

2010

tom 12

nr 2

The recent identification of Myotis brandtii in Ireland raised the possibility that many roosts previously identified as M. mystacinus had the potential of being misidentified M. brandtii. Thus, the distribution and population estimates for M. mystacinus may have been over-estimated, while M. brandtii may have been under-estimated. Results from an all Ireland genetic survey of known M. mystacinus maternity roosts confirm that no long term misidentification has taken place. All specimens caught and sampled were M. mystacinus. Additonally, no further records of M. brandtii were found during six nights of woodland trapping using the acoustic lure. While the status of M. mystacinus in Ireland is now listed as ‘least concern’ in the Irish Red List, M. brandtii is listed as ‘data deficient’ and cannot currently be considered a resident species.

Empirical assessment of non-invasive population genetics in bats: Comparison of DNA quality from faecal and tissue samples

64%

Boston E. S. M., Puechmaille S. J., Scott D. D., Buckley D. J., Lundy M. G., Montgomery I. W., Prodohl P. A., Teeling E. C.

Acta Chiropterologica

2012

tom 14

nr 1

Non-invasive population genetics has become a valuable tool in ecology and conservation biology, allowing genetic studies of wild populations without the need to catch, handle or even observe the study subjects directly. We address some of the concerns regarding the limitations of using non-invasive samples by comparing the quality of population genetic information gained through DNA extracted from faecal samples and biopsy samples of two elusive bat species, Myotis mystacinus and Myotis nattereri. We demonstrate that DNA extracted from faeces and tissue samples gives comparable results for frequency based population genetic analyses, despite the occurrence of genotyping errors when using faecal DNA. We conclude that non-invasive genetic sampling for population genetic analysis in bats is viable, and although more labour-intensive and expensive, it is an alternative to tissue sampling, which is particularly pertinent when specimens are rare, endangered or difficult to capture.

Ograniczanie wyników

3 Acta Chiropterologica

3 Boston E. S. M.

3 Teeling E. C.

2 Buckley D. J.

2 Lundy M. G.

2 Prodohl P. A.

2 Puechmaille S. J.

2 Scott D. D.

1 Bekaert M.

1 Clissmann F.

1 Gager Y.

1 Marnell F.

1 Montgomery I. W.

1 Montgomery W. I.

1 2014

1 2012

1 2010

Wyniki wyszukiwania

Further evidence for cryptic north-western refugia in Europe? Mitochondrial phylogeography of the sibling species Pipistrellus pipistrellus and Pipistrellus pygmaeus

The status of the cryptic bat species, Myotis mystacinus and Myotis brandtii in Ireland

Empirical assessment of non-invasive population genetics in bats: Comparison of DNA quality from faecal and tissue samples