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The aim of the study was to analyse the content and profile of carotenoids, polyphenols content,the capacity to scavenge DPPH radical, the content of neutral detergent fibre, acid detergent fibreand hemicellulose, cellulose and lignin fractions in carrot pomace dried with different methods. Fresh carrot pomace obtained as a by-product during the extraction of juice from Nantejska carrot was usedin tests. Portions of pomace were frozen, dried by convection or lyophilized. The study demonstrateda significant loss of total carotenoids in convective dried (CPC) and lyophilized (CPL) carrot pomace.The greatest decrease was found for β-carotene. The content of phenolics increased by ca. 2.5% forCPC and by ca. 20% for CPL. The capacity to scavenge DPPH radicals was also higher in dry pomace.Significantly higher content of neutral detergent fibre, including hemicellulose fraction, was found inCPC. The analysis did not clearly indicate the optimal drying method. The choice of drying methods hould depend on the type of final product in which carrot pomace will be used
BACKGROUND AND AIMS: The aim of this study was to evoke plasticity in chosen structures of the rat visual system by visual training (sensory experience). METHODS: Local field potentials (LFPs) were recorded from the rat superior colliculus (SC) and primary visual cortex (VCx) of contralateral hemisphere to stimulated eye. LFPs were collected before, during and after visual training. The training consisted of series of 300 repetitions of light flashes separated by 2–3 s intervals. The series of stimuli were presented to the one eye every 15 minutes through three hours. RESULTS: Our data show that visual stimulation significantly enhanced magnitude of visual responses in both recorded structures. A significant increase of visual responses occurred after first hour of training (four stimulating series) both in SC and VCx. The largest increase of VEP amplitudes in the SC was observed after the third hour of stimulation and that was significantly different compared to the first and the second hour of training. Regarding the VCx, advanced alterations of VEPs were observed already after the first hour and then the amplitudes of cortical VEPs remained at a similar level to the end of training. To examine whether the above changes did not result from the changes in the level of anesthesia and global brain state, we considered the VCx LFP power ratio in delta (1–4 Hz) and beta (13–30 Hz) frequency range of the signal recorded for 30 s before each series of stimulation. Changes in the course of delta/beta ratio were similar for all channels in VCx during three hours of visual training and didn’t correlate with increase of VEPs. CONCLUSIONS: Repetitive visual stimulation enhance responses in the visual system, both at cortical and subcortical level, independently of the global brain state, thus may constitute a fundamental approach to improve visual functions. Supported by ERA-NET Neuron project REVIS.
Although the grey wolf was on the brink of extinction in Central Europe in the last century, it never became extinct in Slovakia and nowadays its population is considered stable. The wolf population in Slovakia is estimated to be around 400 individuals with seasonal variations, and due to these small numbers, studies on the parasite fauna of wolf are scarce. Of the 35 parasitic species recorded worldwide in grey wolves in temperate and mountain zones of the Palearctic region, 15 were detected in Slovakia. In our study, 256 grey wolf faeces samples taken from three protected areas in Slovakia were examined using the modified flotation method with a zinc sulphate solution. In total, 169 samples (66%) displayed propagative stages belonging to ten parasitic taxa (Isospora spp., Alaria alata, Taenia spp., Strongyloides stercoralis, Ancylostomatidae, Trichuridae, Toxocara canis, Toxascaris leonina, Spirocerca lupi, Angiostrongylus vasorum). The Trichuridae was the most prevalent group, with a prevalence of 17.7–60.3%. The parasitic species Isospora spp. (3.5%) and A. vasorum (0.8%) are reported for the first time in wolves in Slovakia. Considering the zoonotic potential of some parasites, and the increasing co-existence of human and wildlife in protected areas, the present study provides important findings for further epidemiology research in the grey wolf population.
BACKGROUNS AND AIMS: The study develops methodology of photothrombotic stroke (PtS) induction in cats visual cortex for future studies of post-stroke visual recovery. Considering its well known organisation and similarity to the human, cat visual system is a good model for spontaneous and supported brain reorganisation after ischemia. METHODS: Photothrombosis was used as a model of focal ischemic cortical stroke. Standardisation of the methodology of cortical PtS was performed on four experimental cats with parallel local field potential (LFP) recordings in and around the stroke core – before, during and after the infarct. Intravenously injected Bengal Rose was locally irradiated by cold light via an optic bundle placed on the skull, thinned skull or directly on dura surface. Different light source parameters and irradiation time were tested. Postoperatively isolated brains were preserved and frozen, cut and stained. Final position of the electrodes was monitored on dried 50 μm slices. The cytochrome oxidase (CO) activity and Nissl staining were used to monitor the state of the tissue injury. RESULTS: The aimed unilateral stroke was performed in the dorsal zone of the left marginal gyrus over the visual cortex on a border of the cortical areas 17 and 18. CO visualizes the areas of lower mitochondrial activity in the illuminated tissue of the irradiated cortex in comparison to the contralateral intact homotopic areas. The spontaneous LFP dynamic decreased for at least three hours within the irradiated cortex but not in opposite hemisphere or surrounding tissue. CONCLUSIONS: The 25 minutes of the unilateral irradiation directly to the dura surface with the light temperature 2750K resulted in the most accurate lesion covering all the width of the marginal gyrus and partially the sulcus area not spreading on adjacent gyri or further blood vessels. The infarct reaches the white matter without its pronounced injury. Supported by:ERA-NET:REVIS&MNiSW0292/IP1/2013/72.
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