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Specificity of mass spectrometry (MALDI-TOF) in the diagnosis of Babesia canis regarding to other canine vector-borne diseases

100%

Dziegiel B., Adaszek L., Banach T., Winiarczyk S.

Annals of Parasitology

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2016

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tom 62

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nr 2

The canine vector-borne diseases (CVBD) is a term, which describes a range of infectious or/and parasitic diseases whose etiological agents are transmitted by vectors. CVBD are becoming more widely in the world in relation to global warming and the increasing number of infected vectors. The aim of this study was to assess rapid mass spectrometry (MS) – based proteomics analyses for diagnosis of Babesia canis, Anaplasma phagocytophilum and Borrelia burgdorferi infections in dogs. The study was conducted on four groups of dogs – healthy dogs (group 1, n=10) and dogs infected with B. canis (group 2, n=20), A. phagocytophilum (group 3, n=20) and B. burgdorferi (group 4, n=20) which demonstrated symptoms of the diseases. The MALDI-TOF (Matrix Assisted Laser Desorption Ionization with Time of Flight detector) MS technique revealed the presence of specific protein fraction of 51–52 kDa only in the blood serum of all the animals infected with the B. canis protozoa. The proteins are suspected to be disease markers, whereas the MALDI-TOF technique itself has high specificity and sensitivity and can be applied in the diagnosis of canine babesiosis.

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Autonomic regulation of gastric myoelectrical activity in postmenopausal women supplemented with ovarian hormones

100%

Banach T., Thor P. J., Milewicz T., Krzysiek J.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

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nr 2

3

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

88%

Marek A., Pyzik E., Stepien-Pysniak D., Hauschild T., Banach T.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

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nr 2

The aim of the study was to identify the affinity of 10 Staphylococcus strains isolated from table chicken eggs to specific species. Preliminary analysis performed by API ID32 Staph test identified these strains as S. aureus, but they exhibited a negative reaction in the tube coagulase test. Thus, the analysed strains were initially characterised as Staphylococcus aureus-like (SAL). Further characterisation was performed by genoty pie methods, using restriction fragment length polymorphism (RFLP) of the coagulase gene (coa) and sequencing of the gene rpoB. An attempt was also made to identify the isolated Staphylococcus strains by MALDI-TOF mass spectrometry. The results indicated that none of the strains tested belonged to the species S. aureus. The rpoB sequences of five isolates showed the highest sequence similarity to S. haemolyticus, three isolates to S. chromogenes, and one isolate to S. epidermidis. One strain (SAL4) remained unidentified in this analy sis. The results obtained using mass spectrometry were comparable to those based on gene sequence analysis. Strain SAL4, which could not be identified by sequencing, was identified by MALDI-TOF as Staphylococcus chromogenes.

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Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

88%

Banach T., Adaszek L., Wylupek D., Winiarczyk M., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2013

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tom 16

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nr 3

Proteomics including the studies of the structure, function and dependences between proteins is more and more extensively applied in human medicine and veterinary medicine. The analysis of protein profiles of tissues and body fluid from healthy and ill individuals allows to identify diagnostic, prognostic and predictive markers in various pathological states in people and animals. This paper presents preparation of urine samples for analysis in the mass spectrometer MALDI-TOF (Ultraflextreme, Bruker, Bremen, Germany) by means of two methods: liquid chromatography based on the system Nano-LC (PROTEINER FC II, Bruker Daltonics, Bremen Germany). and two-direction electrophoresis 2DE (GE Healthcare, United Kingdom). Both methods enable separation of the mixture under consideration into individual fractions of high purity indispensable for obtaining readable mass spectra. The purpose of this paper is to determine applicability of these methods in analysis of protein composition of urine samples.

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The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

76%

Adaszek L., Jankowska M., Kalinowski M., Banach T., Wulupek D., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2013

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tom 16

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nr 1

The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

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Peripheral mechanisms of intestinal dysmotility in rats with salsolinol induced experimental Parkinson's disease

76%

Banach T., Zurowski D., Gil K., Krygowska-Wajs A., Marszalek A., Thor P. J.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

Gastrointestinal dysmotility in Parkinson's disease (PD) has been attributed in part to peripheral neurotoxine action. Our purpose was the evaluation of the salsolinol effect on intramuscular interstitial cells of Cajal (ICC), duodenal myoelectrical activity (DMA) and vagal afferent activity (VAA) in rats with experimental PD. Twenty rats were divided into 2 equal groups. Experimental PD was produced in one group by 3 weeks of the intraperitoneal salsolinol injections (50 mg/kg/day), whereas the 2-nd group served as control. DMA and VAA were recorded in both groups during fasting and stepwise - gastric distension (GD) of 10 ml. Subsequently fragments of duodenum were removed and intramuscular ICC were assessed as c-Kit antigen percentage in the duodenal muscular zone. Analyses of the fasting DMA and VAA recordings didn't reveal differences between the compared groups. During GD increase of DMA dominant frequency (p=0.04) and VAA frequency (p<0.01) was observed in the controls whereas in the salsolinol group both parameters remained unchanged. Image analysis of duodenum revealed decreased c-Kit expression in the salsolinol-injected animals (p=0.05). The results of our study may suggest the direct effect of salsolinol on both ICC and neuronal pathways of gastro-duodenal reflexes.

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Peripheral mechanisms of intestinal dysmotility in the morphine tolerant and dependent rats

76%

Banach T., Zurowski D., Gil K., Weisbrodt N. W., Rosenfeld G., Thor P. J.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 1

Changes of intestinal motility and transit produced by tolerance to and dependence upon morphine have been partly attributed to peripheral mechanisms. We evaluated the effect of chronic peripheral morphine administration and peripheral µ-receptor blockade on vagal afferent activity (VAA) and c-Kit positive intramuscular cells of Cajal (ICCs). Ten rats were subjected to chronic subcutaneous morphine infusion for 72 h with subsequent VAA recording. Potential frequency was evaluated within recordings before and after µ receptor blockade by D-Phe -Cys -Tyr -D-Trp -Orn -Thr -Phe -Thr (CTOP) i.p. injections. Afterwards the rats were sacrificed and intramuscular c-Kit antigen expression was assessed by image analysis within removed fragments of duodenum and ascending colon. An equal group of rats served as a control for VAA and c-Kit expression. Analysis of VAA revealed similar frequencies of potentials in morphine tolerant / dependent rats before CTOP and in the controls. CTOP increased potential frequency in the morphine group which effect was visible mostly within the first 20 minutes (p=0.01). The morphine infused animals presented also higher c-Kit expression in both the duodenum (p<0.001) and the ascending colon (p<0.001) in comparison to the control group. Results of our study may indicate the involment of both the intestinal wall and the long vago-vagal reflexes in tolerance to and dependence upon opioids.

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Występowanie zakażeń Bordetella bronchiseptica u psów na Lubelszczyźnie - badania wstępne

64%

Garbal M., Adaszek L., Lyp P., Surma-Kurusiewicz K., Luft-Deptula D., Frymus J., Banach T., Winiarczyk S.

Medycyna Weterynaryjna

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2016

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tom 72

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nr 08

The aim of the study was to present and develop a comprehensive diagnosis of B. bronchiseptica infections in dogs from Lublin Voivodeship and to determine whether these animals were infected with one or multiple genotypes of the bacteria. Thirty-five dogs with bordetellosis confirmed by bacteriological and molecular tests were included in the study. Samples of blood for hematology and biochemistry, as well as bronchial alveolar lavage for cytological and molecular tests, were taken from all animals. Seven dogs showed a decreased hematocrit and a decreased number of red blood cells. In 16 dogs, leukocytosis was found. Increased AST activity, increased ALT activity, elevated serum concentrations of urea, and increased creatinine concentrations were observed in 3, 5, 3, and 3 dogs, respectively. Endoscopy showed inflammation in the trachea and bronchi in 15 dogs. Cytological examination of bronchoalveolar lavages revealed a large number of macrophages (in 18 dogs), lymphocytes (in 8 dogs), and eosinophil granulocytes (in 2 dogs). The genetic material of the bacteria, a fragment of Bordetella spp flaA gene, was detected in nasal and pharyngeal swabs taken from all 35 dogs, and in bronchial alveolar lavage from 17 dogs. A comparison of the nucleotide sequences of the gene showed that the homology between the bacterial isolates obtained in our study was 100%. This suggests the presence of only one B. bronchiseptica genotype in the dog population in Lublin Voivodeship. The sequence similarity of our isolates with sequences available in the gene bank (NCBI PubMed: EU 327790 from China, AJ 012319 from Argentina, L 13034 from the US) was also very high, ranging from 98.2% to 100%. The results of our study indicate that a comprehensive diagnosis of the disease and its constant monitoring are necessary for recognizing the disease, for determining the relationship between the genetic structure of pathogens and the course of the disease, and for studying the immunoprophylaxis of B. bronchiseptica infections in dogs.

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Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of coagulase-negative staphylococci isolated from milk of cows with subclinical mastitis

64%

Banach T., Bocniarz M., Lyp P., Adaszek L., Wawron W., Furmaga B., Skrzypczak M., Zietek J., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 3

The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81.8%). The most frequent cause of subclinical mastitis was found to be Staphylococcus sciuri (39%), while S. vitulinus was detected in 15% of the milk samples. The results obtained indicate that MALDI-TOF MS can be used for the identification of CNS isolated from bovine mastitis as a method supplementary to biochemical tests.

Ograniczanie wyników

Specificity of mass spectrometry (MALDI-TOF) in the diagnosis of Babesia canis regarding to other canine vector-borne diseases

Autonomic regulation of gastric myoelectrical activity in postmenopausal women supplemented with ovarian hormones

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

Peripheral mechanisms of intestinal dysmotility in rats with salsolinol induced experimental Parkinson's disease

Peripheral mechanisms of intestinal dysmotility in the morphine tolerant and dependent rats

Występowanie zakażeń Bordetella bronchiseptica u psów na Lubelszczyźnie - badania wstępne

Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of coagulase-negative staphylococci isolated from milk of cows with subclinical mastitis