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The aim of this study was to explore ecological adaptation of seed microsculptures of Saussurea from different altitudes. Scanning electron microscopy was used to observe the cypselae surface of 10 taxa of Saussurea collected from the eastern edge of Qinghai-Tibet plateau, China. The results indicated that the microsculptures had clear and consistent variations in some samples collected from high (4200 m a.s.l.) and low-altitude sites (2200 m a.s.l.). The thickness of stripes and distance between stripes are increasing while sulcus depth is increasing from a low to a high altitude (P < 0.01). The seed-coat surface was becoming rougher from low to high altitude. The surviving rate of seeds was lower at high altitude than that at low altitude in all the species. The surviving rate of the seeds collected from the high altitudes was higher than that of the seeds collected from low altitudes when sown at the same altitude. All the results suggested that there is selection pressure of the altitude on the development of the microsculpture patterns, which increases absorbed sunlight, the residence time of soil water and nutrition. The changes can make the seeds survive better at extreme environments (cold and dry). The cypselae microsculpture patterns should be used as potential adaptation biomarkers for the species of Saussurea from low- to high-altitude.
The efficiency of a microwave-assisted Fenton-like process using special supported catalysts was evaluated using total organic carbon (TOC) removal from pharmaceutical wastewater. No acidifi cation was required. The highest level of TOC removal efficiency from wastewater achieved was 65.88%. Moreover, the maximum number of consecutive uses with high activity was four. Under optimal conditions, the influent value of BOD5/COD was 0.25, and the effluent value of BOD5/COD was elevated to 0.40. In addition, colour was completely removed. This efficiency was compared with the same MW-Fentonlike process using common supported catalysts, where the highest achieved TOC removal efficiency from wastewater was 39.25%, the colour of the wastewater decreased from 50 to 20, and the value of BOD5/COD was elevated from 0.25 to 0.34. The maximum number of consecutive high activity uses was two. This advanced performance was attributed to no presence of copper carbonate or cerium carbonate on the surface of special supported catalysts. The preparation method for these catalysts combined the merits of the isometric impregnation method with some new improvements. Its advantages include high-efficiency performance, short preparation time, low reagent usage (cupric nitrate 3.6 g, cerium nitrate 1.2 g, ammonia 1 mL), and reusability. The properties of the catalyst with the most efficient performance were characterized by determining surface particle size, the relative amount of active components and promoters, and the stable crystal form of the active components.
Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.
Even though seeds are hidden inside fruit, they differ in color; an interesting, but puzzling, phenomenon. Here, the antioxidant capacities of white, yellow, red and purple seeds from a single variegated maize plant ear were measured and the effects of temperature (15, 30 and 40 C) on germination behavior assessed. The antioxidant capacity of the seeds followed the order: purple[red[yellow[ white. The most favorable temperature for all of the seeds was 30 C. However, the highest germination rates (GRs) were observed in purple seeds, compared to the other colored seeds, when exposed to 40 C, and white seeds, when exposed to 30 C. The germination speeds among the seeds remained consistent (white[yellow[ red[purple), irrespective of temperature. The impaired germination observed at 40 C was significantly improved by adding exogenous antioxidants, but was further impaired by adding H2O2, especially for the white seeds; similar effects on GR were observed at 30 C and 15 C. High negative (at 30 C) and positive (at 40 C) correlations were observed between antioxidant capacity and GR. However, only high negative correlations were detected between antioxidant capacity and germination speed. In addition, no significant difference in heat stress tolerance was observed in the seedlings produced by the different colored seeds. Thus, we speculate that adverse conditions favor the germination of deeper colored seeds, whereas favorable conditions favor lighter colored ones. We discuss the significance of color, from the perspective of seed antioxidant capacity and germination ability under different environmental conditions.
To gain a better understanding of the impact from the land-use change in the Huixian karst wetland system, we analyzed soil microflora, enzyme activities, and physicochemical properties from three land-use types (natural wetland, paddy field, and dry farmland). The results showed that soil pH, soil organic carbon, total nitrogen, cation exchange capacity, exchangeable Ca and Mg, and the cellulase and alkaline phosphatase activities in the dry farmland were significantly lower than those in the paddy field and natural wetland (p<0.05). However, soil pH, soil organic carbon, total nitrogen, cation exchange capacity, exchangeable Ca and Mg, and the cellulase and alkaline phosphatase activities made no significant difference to the paddy field and the natural wetland (p>0.05). Moreover, the soil microbial biomass carbon and nitrogen in the dry farmland were also lower than those in the paddy field and natural wetland, although no significant differences were observed (p>0.05). This suggests that, in the alkali condition, natural wetland with high biomass and weak microbial activity may be an important carbon sink. In the CCA biplot, it can be seen that the natural wetland cluster intersects with the paddy field cluster and the dry farmland cluster in the same quadrant (although the paddy field and the dry farmland clusters are separate). Therefore, we concluded that the natural wetland usually was reclaimed as paddy field or dry farmland directly for agricultural output in the Huixian karst wetland system. The paddy field has a waterlogged condition and shows the similar results to natural wetland, which can be regarded as artificial wetland. In view of the similar ecosystem services by paddy fields as substitutes of natural wetland, if the degradation trend of natural karst wetland can’t be reversed, the paddy field should be preserved in the Huixian karst wetland system for its ecosystem service.
The oofamily Dendroolithidae is a distinct group of dinosaur eggs reported from China and Mongolia, which is characterized by branched eggshell units and irregular pore canals. The ootaxonomic inferences, however, were rarely discussed until now. A colonial nesting site was recently uncovered from the Qinglongshan region, Yunxian, Hubei Province, China. More than 30 dendroolithid egg clutches outcrop on the Tumiaoling Hill, including an extremely gigantic clutch containing 77 eggs. All clutches were exposed in the Upper Cretaceous fluvial-deposited Gaogou For mation. In this study, we emend the diagnosis of the oogenus Placoolithus and assign all dendroolithid eggs from the Tumiaoling Hill to a newly emended oospecies Placoolithus tumiaolingensis that shows greatly variable eggshell microstructure. Moreover, our study also disentangles the previous vexing classification of dendroolithid eggs. We conclude that Dendroolithus tumiaolingensis, D. hongzhaiziensis, and Paradendroolithus qinglongshanensis, all of which were previously reported from Yunxian, should be assigned to the newly emended oospecies Placoolithus tumiaolingensis.
This study presents the effect of metal-enriched rice on concentrations of Pb, Cd, Zn, and Cu in the tissues of chicken. The experimental group of chicken (fed with contaminated rice) accumulated high concentrations of Pb (1.00, 0.73, 0.52, 0.99, and 13.8 mg/kg dw in kidney, liver, muscle, blood and feather, respectively), being 2, 1.2, 3.5, 3, and 3.5 times the corresponding tissue Pb in the control group of chicken. Liver seemed to be the primary tissue for Cd accumulation. High concentrations of Pb and Cu were found in feathers compared to other tissues. Lead concentrations in the muscle and liver of the experimental group and liver Cd concentrations in both experimental and control groups exceeded the maximum permissible limits of Pb and Cd in foods of PR China, respectively. The results indicated that there were significant effects of metalenriched rice on Pb and Cd accumulations in chicken, and this dietary exposure pathway poses a potential health risk to local residents.
The objective of this present investigation was to study the different response on drought tolerance and post-drought recovery for two different genotypes of white clover (a drought-tolerant small-leafed white clover and a drought-sensitive large-leafed white clover) associating with antioxidative enzyme and lignin metabolism. Two materials were exposed to 12 days of drought stress and 6 days of rewatering in a greenhouse. Significantly higher activities of superoxide dismutase, ascorbate peroxidase, catalase, guaiacol peroxidase and root activity as well as lower electrolyte leakage and malondialdehyde levels of the small-leafed in leaves and roots were observed as compared to the large-leafed under drought stress and rewatering. Lignification-related enzymes (polyphenol oxidase, cinnamyl alcohol dehydrogenase) activities of two materials in leaves gradually increased from 0 to 9 days of drought stress; then declined at 12 days of drought stress and after rewatering. After 12 days of drought stress, lignin content of two materials in roots was significantly higher relative to the well-watered. Lignin content of leaves in the small-leafed also increased significantly after 6 days of rewatering, but not in the large-leafed. The small-leafed exhibited more sensitive lignin metabolism and faster lignification in leaves and roots than the large-leafed regarding the response of drought stress. These results indicated that antioxidant efficiency and lignin metabolism played important roles in response to drought stress and rewatering for different white clovers. Comparatively higher reactive oxygen species scavenging system and more sensitive lignin metabolism could be associated with better drought tolerance and post-drought recovery in white clover.
A new agent-based model is proposed to support designers in assessing the evacuation capabilities of passenger ships and in improving ship safety. It comprises models for goal-driven decision-making, path planning, and movement. The goal-driven decision-making model determines an agent’s target by decomposing abstract goals into subgoals. The path-planning model plans the shortest path from the agent’s current position to its target. The movement model is a combination of social-force and steering models to control the agent in moving along its path. The utility of the proposed model is verified using 11 tests for passenger ships proposed by the Maritime Safety Committee of the International Maritime Organization
The influence of arbuscular mycorrhizal fungi (AMF) Rhizophagus irregularis on biomass, root morphological characteristics, water status, gas exchange and ion contents of Populus cathayana Rehder males and females under salt stress was studied in a greenhouse experiment. Sex had no influence on AMF colonization. Salt stress decreased the biomass, modified root characteristics, inhibited photosynthesis, relative water content (RWC), while increased the water use efficiency (WUEi), the ratio of root/shoot (RSR) and salt ion contents within the plants. Males and females differed in response to the salt stress and AMF inoculation. AMF inoculation increased the biomass of root, the biomass of shoot, and decreased RSR, especially in males. The root length, root volume (RV), root surface area, and root tip numbers (RTN) were increased with AMF inoculation in males, while RV and RTN were decreased with AMF inoculation in females. AMF had positive effects on RWC and WUEi in both sexes. Mycorrhizal cuttings also had higher gas exchange capacity, lower salt ion contents than non-mycorrhizal cuttings. The principal component analysis showed that males exhibited a better salt tolerance than females, and AMF inoculation had positive effects on both sexes, especially in males.
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