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Near-infrared spectroscopy (NIRS) can detect soil total phosphorus in agricultural environments. Considering the soil moisture and particle size on total phosphorus prediction, we applied NIRS to the detection of soil samples with different soil moistures and particle sizes. Thus the effect of soil moisture and particle size was analyzed quantitatively and qualitatively. The procedures to remove the effect of soil moisture and particle size on total phosphorus prediction were also described. First, the near-infrared reflectance spectra of soil samples with different soil moistures and particle sizes were obtained and the absorbance values were determined. Next, the original spectra were corrected by using moisture absorbance index (MAI) and hybrid correction to counteract the effects of soil moisture and particle size, respectively. Absorbance of soil samples showed high correlation with soil moisture at wavelengths of 1,450 nm and 1,940 nm. MAI is a tool for normalizing the original spectral data so as to correct for soil moisture. Hybrid correction is based on the superposition of NIR spectra, and a particle size different from that of the original soil samples is generated. This is an effective means of correcting for the effect of soil particle size. Finally, using the corrected absorbance values at eight wavelengths (655, 722, 1,055, 1,255, 1,467, 1,678, 1,890, and 2,246 nm), the soil total phosphorus prediction model was built based on LS-SVM. Compared with the model used for original spectral data, the new model exhibited higher accuracy and stability. Results showed that MAI and hybrid correction are effective for correcting for soil moisture and soil particle size during the prediction of soil total phosphorus.
In this study we investigated the role of NO in Pb-induced yeast cell death. We found that the rate of cell death increased with increasing concentrations of Pb(NO₃)₂ and prolonged exposure to Pb(NO₃)₂. NO production also increased significantly during Pb-induced yeast cell death. An exogenous NO donor increased Pb toxicity to cells, while NO synthesis inhibitors and NO-specific scavengers alleviated Pb toxicity. To further investigate the mechanism of NO in Pb toxicity, we measured the Pb content and mRNA expression of metal ion transport gene SMF1 in yeast cells. Our results showed that endogenous NO may enhance SMF1 expression, thereby increasing Pb content in yeast cells. Meanwhile, we found that the intracellular ROS levels increased with the increase of intracellular Pb content in yeast cells. Conclusion: Pb can promote the increase of intracellular nitric oxide levels. NO may promote intracellular Pb transport by enhancing the expression of SMF1 in Pb-induced yeast cell death. Intracellular accumulation of Pb further promotes the increase of intracellular ROS levels, and then lead to yeast cell death by oxidative damage.
A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.
This paper is concerned with sampled-data leader following consensus of multiple unmanned surface vehicle (MUSV) systems with random switching network topologies and wave-induced disturbance. By modelling the switching of network topologies with the use of a Markov process and considering the effect of wave-induced disturbance, a new sampled-data consensus control protocol is proposed. By employing an appropriate Lyapunov-Krosovskii function method and the weak infinitesimal operation, a novel stability criterion is derived, which ensures that the MUSV system can reach robustly leader-following consensus with H∞ performance satisfied. Based on this criterion, the Markov dependent switching consensus controller gains are obtained by solving a set of linear matrix inequalities. Finally, an illustrative example is given to verify the effectiveness of the proposed control scheme for MUSV systems
Background: We investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms. Methods: Hepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p. Results: The MTT assay showed that loureirin B treatment significantly inhibited the proliferation of HSCs in time- and dose-dependent manners. Loureirin B significantly promoted the apoptosis of HSCs, increased the expression of Bax and decreased the Bcl-2 level. Western blot analysis showed that the expressions of Wnt1 and β-catenin were obviously lower in the loureirin B treatment group than in the control group. We also found that loureirin B could decrease the Wnt1 mRNA level and increase miR-148-3p expression. Knockdown of miR-148-3p using inhibitor could reverse the effects of loureirin B on the proliferation and apoptosis of HSCs and the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Conclusion: Our results suggest that loureirin B inhibited the proliferation and promoted the apoptosis of HSCs, and suppressed the Wnt/β-catenin signaling pathway via regulation of miR-148-3p.
The provinces of China have suffered from severe PM2.5 pollution in recent years, presenting a significant threat to human health. Identifying associations between mortality rate and PM2.5 level is extremely useful for a range of purposes, such as the development of preventive measures, increasing health awareness, and establishing disaster warning systems. Based on remote sensing data, station monitoring data, and statistical data, this paper uses the exposure response function, regression analysis, and kriging to evaluate the number of deaths in China’s 31 provinces caused by PM2.5 pollution in 2015. Variations in the number of deaths and mortality rates in China under different PM2.5 concentration control standards have been simulated by a range of countries and organizations helping to develop optimal control standards for each province individually according to actual PM2.5 concentration. These results show that: 1) PM2.5 pollution has an important effect on the mortality rate in China. The rate caused by PM2.5 pollution in 2015 accounted for 1.75‰, or approximately 2.62 million people and 31.14% of all deaths in China. 2) Strict control standards for PM2.5 concentration can bring significant health benefits, with projections that if PM2.5 concentration in China’s provinces were controlled to the level set by China, the EU, Japan, USA, and Australia, the number of deaths caused by PM2.5 pollution would be reduced by approximately 0.95, 1.52, 2.02, 2.26, and 2.49 million people, respectively, or 36.24%, 58.08%, 79.91%, 86.47%, and 95.20% compared with baseline year data. 3) Choosing appropriate control targets for limiting PM2.5 concentrations in different provinces in China is an effective way to obtain optimal health benefits. Beijing, Tianjin, Hebei, Shandong, and Henan should adopt a 35 μg/m3 standard with a 25 μg/m3 standard appropriate for Shanxi, Liaoning, Jilin, Shanghai, Jiangsu, Zhejiang, Anhui, Hubei, Hunan, Chongqing, Shanxi, and Xinjiang; 13 provinces, including Inner Mongolia, Heilongjiang, Fujian, Jiangxi, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan, Tibet, Gansu, Qinghai, and Ningxia, should adopt the 15 μg/m³ standard; and Hainan should consider choosing a 12 μg/m³ standard.
The Arabidopsis Ethylene-Insensitive 2 (EIN2) gene has been shown to be involved in mediating the oxidative stress response; however, little is known about the underlying mechanisms involved. In this study, we found that the ethylene-insensitive mutant ein2-1 showed enhanced tolerance to oxidative stresses caused by both paraquat (PQ) and hydrogen peroxide as well as alleviated oxidative damage. Moreover, higher transcript levels of a choroplast Cu/Zn superoxide dismutase gene CSD2 and a catalase gene CAT3 and, consequently, higher activities of superoxide dismutase (SOD) and catalase (CAT), were detected in ein2-1 plants than in wild-type plants in the abtence or presence of PQ. These retults suggest that the ein2-1mutation results in constitutive activation of CSD2 and CAT3 genes and increases in the activities of SOD and CAT and, consequently, enhanced oxidative stress tolerance.
Bacteriocin E50-52, a class IIa bacteriocin with a wide antibacterial spectrum, and has a huge potential to be a substitute for conventional antibiotics. In this research, the bacteriocin E50-52 gene was cloned into the expression vector pET SUMO (small ubiquitin-related modifier) and introduced into Escherichia coli BL21 (DE3). The recombinant fusion protein SUMO-bacteriocin E50-52 expressed in a soluble form was purified to a purity of more than 90% by Ni-NTA sepharose column and 117 mg fusion protein was obtained per liter of fermentation culture. The fusion protein was cleaved with SUMO protease and re-applied to a Ni-NTA Sepharose column. Finally, about 16 mg recombinant bacteriocin E50-52 (rbE50-52) was obtained from a 1-liter fermentation culture with no less than 95% purity. The rbE50-52 had similar antimicrobial properties and molecular weight as the native bacteriocin E50-52 and showed very low hemolytic activity.
Based on specimen examination and previous records, we provide a checklist including 146 species from 51 genera and 10 tribes of the weevil subfamily Ceutorhynchinae in China. Of these species, the following 18 are reported in China for the first time: Amalus scortillum (Herbst, 1795), Ceutorhynchus filiae Dalla Torre, 1922, Dieckmannius ingensColonnelli, 1993, Dieckmannius sexnotatus (A. Schultze, 1899), Nedyus quadrimaculatus (Linnaeus, 1758), Zacladus stierlini (A. Schultze, 1902), Auleutes epilobii (Paykull, 1800), Hypurus bertrandi (Perris, 1852), Hypurus portulacae (Marshall, 1916), Neoplatygaster venusta (Faust, 1885), Belonnotus tenuirostris (Marshall, 1917), Mecysmoderes fulvus (Roelofs, 1875), Xenysmoderes stylicornis (Marshall, 1934), Mononychus ireos (Pallas, 1773), Rhinoncus jakovlevi Faust, 1893, Rutidosoma globulus (Herbst, 1795), Rutidosoma graminosum (Gistel, 1857) and Rutidosoma koreanumKorotyaev & Hong, 2004.
STAT5 is a key intracellular mediator of prolactin signaling and can activate transcription of milk proteins in response to prolactin. STAT5 genes are suggested to be candidate markers for milk protein yield and composition in dairy cattle. PCR-SSCP was applied to analyze the polymorphisms of two loci in STAT5A gene in 279 Chinese Holstein cattle. Genotype frequencies, allele frequencies and correlation coefficients between the polymorphic variants and milk production traits were estimated. Three genotypes were found at the two loci. At locus P1 the frequencies of genotypem AA/GG/AG were 0.240/0.147/0.613 and those of alleles A and G were 0.547 and 0.453, respectively. The A/G genotypes had significant effect on milk yield and milk protein content in lactations 1 and 2. At locus P2 the genotype frequencies of CC/TT/CT were 0.752/0.004/0.244 and C and T allele frequencies were 0.875 and 0.125, respectively. Different genotypes had remarkable effect on the milk protein content in lactation 2.
Soil respiration plays a crucial role in global carbon cycling of terrestrial ecosystems. Changes in atmospheric CO₂ and nitrogen (N) addition across the globe are likely to affect soil respiration. However, the effects of elevated CO₂, and N addition on soil respiration are not fully understood especially in wetland ecosystems. To evaluate the effects of atmospheric CO₂ and N availability on soil respiration, a paired, nested manipulative in situ experiment was performed, using CO₂ fumigation within Open-Top Chambers as the primary factor, and N (as NH₄NO₃) as the secondary factor in a temperate wetland in northeastern China in 2010 and 2011. CO₂ fumigation significantly enhanced soil respiration, according to repeated-measures ANOVA, and the stimulatory effect of CO₂ fumigation on soil respiration was sustained during the whole experimental period regardless of N addition. However, the positive soil respiration effect of N addition alone weakened over time. Moreover, there was a significant interaction between CO₂ fumigation and N addition. Soil temperature explained 50–66% of the variation in soil respiration. Moreover, soil respiration was positively correlated with the root N content and litter decomposition rate. The results suggested that elevated CO₂ concentrations will accelerate soil respiration and ecosystem carbon cycling, thus, limiting soil carbon sequestration, especially when coupled with increasing N deposition.
Considering the characteristics of rural domestic sewage in central China, an unpowered complex ecotechnology composed of anaerobic baffled reactor (ABR), composite soil filling (CSF) system, and free water surface (FWS) constructed wetland was developed to treat rural sewage. The Guogang Village sewage treatment demonstration project at Xinmi City showed that the complex ecosystem had remarkable pollutant removal efficiency. The removal rate was over 90% for chemical oxygen demand and total phosphorus, and over 70% and 80% for TN and NH4₄⁺-N, respectively. The effluent concentration met the integrated wastewater discharge standard of China (GB18918-2002) level 1B. The complex eco-technology could effectively purify water quality, and it was cheaper and had simpler operation. Thus, it could be considered as an effective method for treating rural domestic sewage.
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