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Wydalanie 17-ketosterydów w moczu mężczyzn z przewlekłym alkoholizmem

100%

Konarska L., Gorecki T.

Diagnostyka Laboratoryjna

|

1971

|

tom 07

|

nr 4

2

Pomiar zawartości błonnika w kale

81%

Tomaszewski L., Tadeusiak W., Konarska L., Czarnecka H.

Diagnostyka Laboratoryjna

|

1981

|

tom 17

|

nr 4-5

3

Can arginase be a marker of the large bowel neoplasia?

80%

Konarska L., Kolasa T., Albrecht P., Regula A.

Acta Biochimica Polonica

|

1993

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tom 40

|

nr 1

4

Arginase activity alterations in peripheral blood lymphocytes in the human chronic lymphocytic leukemia

80%

Konarska L., Widzynska I., Zienkiewicz H., Sulek K.

Acta Biochimica Polonica

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1993

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tom 40

|

nr 1

5

Beta-glukuronidaza

80%

Konarska L.

Postępy Biochemii

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1971

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tom 17

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nr 1

6

Comparative studies of nuclear DNA content in benign and malignant thyroid lesions

61%

Konarska L., Skierski J., Ellert A., Steinbrich J., Wozniak A., Kalczak M.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 3

Currently available data suggest that DNA aneuploidy is associated with aggressive behavior of and unfavorable prognosis in several malignant human tumors as compared with diploid malignancies. However, the diagnostic and prognostic importance of flow cytometric DNA measurements in the case of thyroid neoplasms remains controversial. Therefore, the aim of our study was to evaluate utility of DNA index (DI) and proliferative index (PI) in distinguishing benign from malignant thyroid lesions taking into account the possible influence of intra-tumor heterogeneity and tissue preparation mode on DNA flow-cytometry measurements. A retrospective study was performed on 71 paraffin-embedded specimens from 57 patients with benign and malignant thyroid pathologies: 13 colloid goitres, 12 parenchymatous goitres, 19 adenomas and 13 carcinomas. In 14 of 57 cases two separate specimens taken from different areas of the same lesion were analysed and DNA parameters were compared. Additionally, flow cytometry DNA analysis was parallelly performed on 3 adjacent but differently processed tissue sections (fresh, formalin-fixed and paraffin-embedded) taken from each of 26 surgically excised thyroid lesions. DNA content was also analysed in both fresh and formalin-fixed twin specimens of normal pig thyroid glands (N = 6). We demonstrated that all tumors diagnosed as thyroid carcinomas were associated with abnormal nuclear DNA content although aneuploidy was not found specific to malignant thyroid tumors. Aneuploid samples of benign thyroid lesions exhibited higher proliferative activity, expressed as mean PI values, than diploid ones. In carcinomas the mean PI values were significantly higher than in benign lesions, independently whether they concerned aneuploid or diploid tissues. Considering intra-tumor heterogeneity, the flow cytometric DNA parameters can be assumed as reproducible despite differences in the mode of tissue fixation and preparation for analysis.

Ograniczanie wyników

3 Acta Biochimica Polonica

2 Diagnostyka Laboratoryjna

1 Postępy Biochemii

6 Konarska L.

1 Albrecht P.

1 Czarnecka H.

1 Ellert A.

1 Gorecki T.

1 Kalczak M.

1 Kolasa T.

1 Regula A.

1 Skierski J.

1 Steinbrich J.

1 Sulek K.

1 Tadeusiak W.

1 Tomaszewski L.

1 Widzynska I.

1 Wozniak A.

1 Zienkiewicz H.

1 2001

2 1993

1 1981

2 1971

Wydalanie 17-ketosterydów w moczu mężczyzn z przewlekłym alkoholizmem

Pomiar zawartości błonnika w kale

Can arginase be a marker of the large bowel neoplasia?

Arginase activity alterations in peripheral blood lymphocytes in the human chronic lymphocytic leukemia

Beta-glukuronidaza

Comparative studies of nuclear DNA content in benign and malignant thyroid lesions