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1

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

100%
Qin Y., Tian Y.-P.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 2
The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in humans. We determined the global gene expression profile of peripheral whole blood from normal adult male rats after long-term GH gene therapy using CapitalBio 27 K Rat Genome Oligo Arrays. Sixty one genes were found to be differentially expressed in GH gene-treated rats 24 weeks after receiving GH gene therapy, at a two-fold higher or lower level compared to the empty vector group (p < 0.05). These genes were mainly associated with angiogenesis, oncogenesis, apoptosis, immune networks, signaling pathways, general metabolism, type I diabetes mellitus, carbon fixation, cell adhesion molecules, and cytokine-cytokine receptor interaction. The results imply that exogenous GH gene expression in normal subjects is likely to induce cellular changes in the metabolism, signal pathways and immunity. A real-time qRT-PCR analysis of a selection of the genes confirmed the microarray data. Eight differently expressed genes were selected as candidate biomarkers from among these 61 genes. These 8 showed five-fold higher or lower expression levels after the GH gene transduction (p < 0.05). They were then validated in real-time PCR experiments using 15 single-treated blood samples and 10 control blood samples. In summary, we detected the gene expression profiles of rat peripheral whole blood after long-term GH gene therapy and screened eight genes as candidate biomarkers based on the microarray data. This will contribute to an increased mechanistic understanding of the effects of chronic GH gene therapy abuse/misuse in normal subjects.
2

Pontigulasia pentangulostoma nov.spec., a new testate amoeba from the Da Jiuhu peatland of the Shennongjia Mountains, China

81%
Qin Y., Xie S., Gu Y., Zhou X.
Acta Protozoologica
|
2008
|
tom 47
|
nr 2
155-160
3

Ecological service evaluation: an empirical study on the Central Loess Plateau, China

81%
Li Y., Zhang P., Qin Y.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 2
4

Supercooling points of Apis mellifera ligustica when performing different age-related tasks

81%
Li Y., Wang S., Qin Y.
Journal of Apicultural Science
|
2012
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tom 56
|
nr 1
5

Death effects assessment of PM2.5 pollution in China

81%
Xie Z., Qin Y., Zhang L., Zhang R.
Polish Journal of Environmental Studies
|
2018
|
tom 27
|
nr 4
The provinces of China have suffered from severe PM2.5 pollution in recent years, presenting a significant threat to human health. Identifying associations between mortality rate and PM2.5 level is extremely useful for a range of purposes, such as the development of preventive measures, increasing health awareness, and establishing disaster warning systems. Based on remote sensing data, station monitoring data, and statistical data, this paper uses the exposure response function, regression analysis, and kriging to evaluate the number of deaths in China’s 31 provinces caused by PM2.5 pollution in 2015. Variations in the number of deaths and mortality rates in China under different PM2.5 concentration control standards have been simulated by a range of countries and organizations helping to develop optimal control standards for each province individually according to actual PM2.5 concentration. These results show that: 1) PM2.5 pollution has an important effect on the mortality rate in China. The rate caused by PM2.5 pollution in 2015 accounted for 1.75‰, or approximately 2.62 million people and 31.14% of all deaths in China. 2) Strict control standards for PM2.5 concentration can bring significant health benefits, with projections that if PM2.5 concentration in China’s provinces were controlled to the level set by China, the EU, Japan, USA, and Australia, the number of deaths caused by PM2.5 pollution would be reduced by approximately 0.95, 1.52, 2.02, 2.26, and 2.49 million people, respectively, or 36.24%, 58.08%, 79.91%, 86.47%, and 95.20% compared with baseline year data. 3) Choosing appropriate control targets for limiting PM2.5 concentrations in different provinces in China is an effective way to obtain optimal health benefits. Beijing, Tianjin, Hebei, Shandong, and Henan should adopt a 35 μg/m3 standard with a 25 μg/m3 standard appropriate for Shanxi, Liaoning, Jilin, Shanghai, Jiangsu, Zhejiang, Anhui, Hubei, Hunan, Chongqing, Shanxi, and Xinjiang; 13 provinces, including Inner Mongolia, Heilongjiang, Fujian, Jiangxi, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan, Tibet, Gansu, Qinghai, and Ningxia, should adopt the 15 μg/m³ standard; and Hainan should consider choosing a 12 μg/m³ standard.
6

Optimal allocation of control targets for PM2.5 pollution in China’s Beijing-Tianjin-Hebei regions

81%
Xie Z., Li Y., Qin Y., Zheng Z.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
Based on the data of population size, industrial development, energy consumption and technological level from 2013 in the Beijing-Tianjin-Hebei region, the fairness of PM₂.₅ pollution emissions were analyzed using Gini coefficient and contribution coefficient. In addition, a reduction allocation plan of PM₂.₅ concentration by 2020 in 13 cities was determined according to a minimized model of Gini coefficient model. The results showed that: (1) Gini coefficients of industrial development and technological level were greater than 0.4, implying that the PM₂.₅ pollution emissions in the region were significantly unfair from the perspective of industry and technology, especially in the aspect of technological level. (2) The spatial distribution of industrial and technological contribution coefficients presented a downward trend from Beijing and Tianjin to the periphery, and cities in Hebei Province were central to the unfairness of PM₂.₅ pollution emissions across the whole region. (3) The reduction values of PM₂.₅ concentration were between 16.7 1μg/m³ and 57.49 μg/m³, and a reduction allocation plan of PM₂.₅ concentration could improve the overall fairness of PM₂.₅ pollution emissions in the Beijing-Tianjin-Hebei region, but the unfairness of PM₂.₅ pollution emissions would not change dramatically.
7

Effects of land use-cover change in the desert oasis system on topsoil carbon and nitrogen (middle of Heihe River basin, China)

81%
Niu R.-X., Zhao X.-Y., Liu J.-L., Qin Y.
Polish Journal of Ecology
|
2013
|
tom 61
|
nr 1
Land use/cover change (LUCC) is one of the main factors that influence the terrestrial carbon (C) and nitrogen (N) cycle. We examined the effects of land use/cover change on topsoil C, N, and microbial biomass C, N (MBC, MBN) and their relationship with other soil properties in the middle of Heihe river basin along a land use change gradient of 100-year farmland, 27-year farmland, 33-year pine forest, 28-year poplar forest, and 21-year shrubland, as well as native desert from which all the above cultivated systems are converted. Results revealed that land use conversion from native desert to the above cultivated ecosystems not only changed the basic eco-hydrological factors of the soil, such as improving the soil moisture and field capacity, decreasing the pH and salinity, but also altered the nutrient factors, such as improving the concentrations of soil organic C (SOC), total N (TN), MBC, MBN, NO₃⁻ -N and NH₄⁺ -N,. With the increase of cultivated years, land use conversion had an increasing impact on the C and N sequestration and soil nutrients stabilization.
8

Temporal-spatial variation characteristics of soil erosion in the Pisha sandstone area, Loess Plateau, China

81%
Li Y., Xie Z., Qin Y., Sun Y.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 4
Soil erosion in the Pisha sandstone area of the Loess Plateau in China has become a severe environment issue that has raised concerns globally. The projects of ecological restoration in this area and their impact on soil erosion have been analyzed using the unmanned aerial vehicle remote sensing system (UAVRSS) and the revised universal soil loss equation (RUSLE) in the Two-Tiger Valley Basin in 2013 and 2015. Our findings show that: 1) The volume of soil erosion and average soil erosion modulus decreased from 126.24 t year-1 and 6465.295 t km-2 year−1 to 114.7 t year-1 and 6333.19 t km-2 year−1 between 2013 and 2015, respectively. 2) Spatial-temporal variations of soil erosion are extremely significant. All erosion grades recorded different degrees of decline across the study period, except for the level of severe erosion. 3) There is a significant positive correlation between slope degree and soil erosion. When the slope degree was <5°, the soil erosion modulus was 51.355 t km-2 year−1, accounting for only 0.87 % of total erosion in this area. When the slope degree was >35°, the soil erosion modulus attained 2574.413 t km-2 year−1, and the erosion amount accounted for 43.52% of total erosion. Although anti-erosion and the promotion of plant growth measures have achieved noticeable ecological benefits, the present situation of preventing and controlling soil and water loss is still severe.
9

Latitudinal diversity gradients in free-living microorganisms – Hoogenraadia a key genus in testate amoebae biogeography

81%
Bobrov A., Qin Y., Wilkinson D. M.
Acta Protozoologica
|
2015
|
tom 54
|
nr 1
10

Effects of livestock exclusion on soil physical and biochemical properties of a desert rangeland

71%
Qin Y., Niu D., Kang J., Zhou Y., Li X.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 6
11

Morphological redescription and morphogenesis of Urosoma macrostyla (Wrześniowski, 1866) Berger, 1999 (Ciliophora, Hypotrichida)

71%
Qin Y., Qiu Z., Shao C., Warren A., Shen Z.
Acta Protozoologica
|
2011
|
tom 50
|
nr 3
12

Effects of gibberellins A4 on budbreak, antioxidant enzymes’ activity and proline content of flower buds in sweet cherry (Prunus avium)

61%
Cai B., Wang H., Liu T., Zhuang W., Wang Z., Qu S., Qin Y.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 06
Chilling accumulation is required for deciduous trees to maintain its normal growth and development. However, warm winters appeared more and more frequently, which hindered severely the normal growth and yield of fruit trees. To avoid these problems, artificial dormancy breaking agents are widely used. In our study, the effect of mineral oil, hydrogen cyanamide and gibberellins A₄ (GA₄) on budbreak were evaluated in sweet cherry (Prunus avium), which indicated that GA₄ treatment hastened the date of flower budbreak and increased the percentage of budbreak compared with the other treatments. To better understand the mechanism of GA₄-induced budbreak, the contents of antioxidant enzymes’ activity and proline in sweet cherry flower buds treated with GA₄ and water were evaluated. There was a sharp decrease of catalase (CAT) activity after 5 days of GA₄ treatment and then an increase of CAT activity after 10 days of GA₄ treatment. However, there was a sharp increase of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) after 5 days of GA₄ treatment and then a decrease of these enzyme activities after 10 days of GA₄ treatment. There was a transient increase in hydrogen peroxide (H₂O₂) content and proline content after 5 days of GA₄ treatment and a decrease after 10 days of GA₄ treatment. However, there were only small fluctuations after water treatment, which indicated that temporary oxidative stress may contribute to the dormancy release of flower buds in the sweet cherry treated with GA₄ through the regulation of H₂O₂ content coincided with the changes of antioxidant defense system.
13
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Rapid and specific detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation combined with loop-mediated isothermal amplification

61%
Qin Y., Puthiyakunnon S., Zhang Y., Wu X., Boddu S., Luo B., Fan H.
Polish Journal of Food and Nutrition Sciences
|
2018
|
tom 68
|
nr 2
Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplifi cation (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with antiEHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identifi ed by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specific gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specificity and sensitivity, with a positive detection rate of amplifi cation of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 101 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×101 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specific gene amplifi cation technology that is suitable for implementing as a screening assay in basic laboratory and field test for detecting food contamination.
14

How Chlorella sorokiniana and its high tolerance to Pb might be a potential Pb biosorbent

61%
Liang S., Kang Y., Zeng L., Qin Y., Wang L., Zhang Q., Luo J.
Polish Journal of Environmental Studies
|
2017
|
tom 26
|
nr 3
The link between the acute toxicity of heavy metals on algae and the bioadsorption capacity of heavy metals by algae has seldom been reported. In the present study, an acute toxicity experiment was carried out to assess the toxic effects of Pb, Cu, and Cd for Chlorella sorokiniana, and the 96 h IC₅₀ values were 0.249 mg/L, 0.485 mg/L, 46.108 mg/L, and 21.00 mg/L for Cu, Cd, Pb (total), and Pb (free ion), respectively, which implied that Chlorella sorokiniana showed high tolerance to Pb compared to Cu and Cd. Pb distribution analysis indicated that 73.40% to 98.15% of free Pb ions were accumulated on the algae cell wall to avoid further intracellular accumulation, resulting in irreversible metabolic disorders. Then the adsorption capacity of Chlorella was explored. It could be found that the Langmuir model (the R² were 0.988 and 0.962 for living and lifelss cells, respectively) was fit to explain the adsorption equilibrium data and the qₑ calculated by this model were 1.54 and 2.97 mg /10¹⁰ cells for living and lifeless cells, respectively, which was consistent with the experimental result. In the competitive adsorption, Chlorella exhibited a greater affinity for Pb with the higher removal rate compared to Cu and Ni. Therefore, the renewable Chlorella sorokiniana and its dramatic resistance to Pb may serve as a potential biosorbent for Pb in the future.
15

Reduction and enrichment of uranium after biosorption on inactivated Saccharomyces cerevisiae

51%
Zhang W., Dong F., Liu M., Song H., Nie X., Huo T., Zhao Y., Wang P., Qin Y., Zhou L.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 2
16

VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance

51%
Dong C., Zhang Z., Qin Y., Ren J., Huang J., Wang B., Lu H., Cai B., Tao J.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 10
To understand cold acclimation and cold tolerance in Vitis, we isolated a C-repeat binding factor 1 (CBF1) transcriptional activator from cold-sensitive Vitis vinifera ‘Manicure Finger’ and cold-tolerant wild Vitis amurensis. Under cold stress, the CBF1 transcript accumulation of V. amurensis increased, whereas that of V. vinifera showed no significant change. The transcript levels of VaCBF1 in the roots, stems, leaves, and petioles under cold stress were up-regulated in a time-dependent manner. The transcript level of VaCBF1 in the leaves was induced by salinity stress or by exogenous abscisic acid and salicylic acid. The presence of the cis-elements MBS, MYB, and MYC in the VaCBF1 promoter suggests that this promoter is a component of the CBF transduction pathway, which is involved in plant response to cold stress. The overexpression of VaCBF1 increased the cold tolerance of transgenic tobacco at −4 °C. The transcript level of the downstream target gene NtERD10D appeared in the transgenic lines under normal conditions, whereas that of NtERD10D and NtDREB3 improved under low temperature. We suggest that VaCBF1 enhances stress tolerance by increasing antioxidant activities and promoting downstream target gene expression.
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