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The application of phenological data together with meteorological and pollen data in a comprehensive analysis gives an opportunity to draw conclusions on variability of the starting date of the pollen season and its dynamics in terms of meteorological factors. It is quite important especially due to the fact that studies conducted all over Europe have proved that species phenology responds to climate warming trends. There has been observed a tendency to an earlier onset of spring flowering and leafing as well as the lengthening of the growing season. Although phenological network studies differ with regard to regions, species, events observed and applied methods, their data show a clear temperature-driven extension of the growing season by up to 2 weeks in the second half of the 20th century in mid- and high northern latitudes; for example, in Germany changes in timing of phenological spring events have been estimated at about -1.6 days / decade, while in Switzerland: -2.3 days / decade. Despite interannual variability in flowering date, caused by specific meteorological conditions each year, long-time series of phenological data from the area of Poland have proved that hazel flowering occurred in the surroundings of Warsaw later in the 50’s (third decade of March) than it is observed at the beginning of the 21st century (second decade of March). There is a lack of such long time series of pollen data, but we can suspect that the hazel pollen season has changed similarly to the time pattern of its flowering. Plants are very sensitive to weather conditions, therefore it is important to know as precisely as possible the impact of meteorological conditions on a plant’s reactions. The determination of thermal thresholds for a specific plant’s reactions may be beneficial for this purpose. The estimated value of Positive Degree Days (PDD> 50), which caused the first Corylus flowers (F2 phenophase) to bloom in the study years, requires testing in future years to make the threshold values credible.
INTRODUCTION: The ketogenic diet (KD) is high fat and low carbohydrate diet type. Ketogenic metabolism is characterized by moderate hypoglycemia. It also leads to the use of ketone bodies as an alternative source of energy for the organism. In the last decade, the influence of various factors on the gut microbiota has been noticed. The gut microbiota includes bacteria as well as fungi, archaea, viruses, and protozoa. Gut microbiota has been shown to participate in the regulation of processes in the central nervous system via the gut-brain axis. Differences in the composition of gut microbiota can affect the proper functioning of the central nervous system. It seems that KD can affect the gut microbiota composition. AIM(S): The aim of the study was to evaluate the influence of two types of KD high in animal fat on the number of Enerococcus faecalis and Escherichia coli in faeces of 129SV mice. METHOD(S): Ten male 129SV mice were divided into two groups and fed for 28 days with a KD high in animal fat with different ratios (4: 1 and 6: 1). The assessment of the number of bacteria was performed on 0, 14, and 28 days of feeding with the KD by real time‑QPCR. Additionally, body mass and β‑hydroxybutyrate levels were measured. RESULTS: An increase of the amount of Enterococcus faecalis and Escherichia coli after 14 and 28 days in the faeces of 129SV mice fed with both KDs was observed. The results showed a decrease in the body mass of mice fed with KD with ratio of 4: 1. Changes in body mass of mice fed with KD with a ratio of 6: 1 has not been observed. The increase of β‑hydroxybutyrate levels in blood of mice fed with both KDs has been noticed. CONCLUSIONS: In conclusion, results of evaluation of the number of Enterococcus faecalis and Escherichia coli may suggest that a KD high in animal fat affects the gut microbiota composition in 129SV mice
INTRODUCTION: The ketogenic diet (KD) is used to manage drug‑resistant epilepsy in children. The KD directs the metabolism towards fat consumption as an alternative source of caloric demand, thus a state similar to starvation is created. The gut‑brain axis is associated with biochemical signaling between the large intestine and the nervous system via gut microbiota. It seems that, for an efficient functioning of the brain, a well-developed gut‑brain axis is required. Growing evidence supports the participation of gut microbiota in the regulation of cognitive functions, mood, anxiety, and pain. The probability of the KD affecting the composition of gut microbiota has been shown. One of the important aspects is the right choice of animal model for research. Animal models are not equal in metabolic, biochemical, physiological, and behavioral conditions. It is important to know the microbiota diversity in animal models. AIM(S): The aim of this study was a quantitative and comparative analysis of Enterococcus faecalis bacteria in the faeces of Wistar and Long Evans rats fed with KD of two different compositions. METHOD(S): Ten male Wistar rats and ten male Long Evans rats were divided into two groups. Animals were fed the KD based on animal or plant fat for 28 days. Subsequently, real‑time PCR was performed to assess the number of Enterococcus faecalis. Body mass and β‑hydroxybutyrate levels were measured as well. RESULTS: It has been observed that, after 28 days, the number of Enterococcus faecalis in faeces of rats fed with the KD was increased. Furthermore, results showed an increase in body mass and β‑hydroxybutyrate levels in both Wistar and Long Evans rats. CONCLUSIONS: In conclusion, analysis of changes in the number of bacteria Enterococcus faecalis has shown that the KD may alter gut microbiota composition in Long Evans and Wistar rats fed with the KD.
Phenology is primarily seen as an indicator of the impacts of climate change. The strongest biological signal of climatic change is revealed by phenological data from the period after 1990. Unfortunately, the Polish nationwide network of phenological monitoring was terminated in 1992, and was only reactivated in 2005. Here, we attempt to reconstruct late spring phenophases of flowering of Syringa vulgaris L. and Aesculus hippocastanum L. across several sites in Poland from 1951 to 2014 using the GIS-based approach (if observations from neighboring stations were available) and multiple regression modeling with stepwise screening and bootstrap resampling. It was found that the air temperature and its indices explain over 60% of the variance, giving an accuracy of 3.0-3.4 days (mean absolute error) and correlation coefficients of 0.83 and 0.78 for lilac and horse chestnut, respectively. Altogether, both plant species showed a statistically significant advancement in the onset of flowering with an average rate of 1.7 days per decade. We also found that the final trend is the result of rapid acceleration of the increase in air temperature after the 1990s, while most of the trends for late spring were ambiguous before that period.
INTRODUCTION: High fat diets are characterized by a high fat content with an adequate proportion of proteins and carbohydrates. For a long time, the ketogenic diet has been used in the treatment of epilepsy; however, its neuroprotective properties indicate its putative and broader application in the treatment of neurodegenerative diseases and neurodevelopmental disorders. AIM(S): The present study is focused on the selection of a proper animal model in the preclinical studies on ketogenic therapeutic potential. METHOD(S): The study involved 160 male mice from 129/SV and C57 BL/6 strains at the age of 9 weeks. The animals were divided into 4 groups. The control groups have been fed with a standard diet and the experimental groups were fed with three different types of a high fat diet: (A) a diet imitating a classic ketogenic diet composed mainly of saturated fats of animal origin, where the ratio of fat to carbohydrates and proteins is 4:1, (B) a diet imitating a modified ketogenic diet with a high content of unsaturated fat of vegetable origin, where the ratio of fat to carbohydrates and proteins is 4:1, or (C) a diet imitating ketogenic diet, containing fats of animal origin where the ratio of fats to carbohydrates and proteins is 6:1. On the 100th day of the experiment, the animals of all groups were subjected to motor and behavioral tests, including: an open field test, elevated plus maze, test on a raised treadmill, and a Grip Test. After testing, the animals were euthanized to collect material for further analysis. CONCLUSIONS: The collected samples will be used to determine the level of expression of selected genes and the level of neurogenesis in the hippocampal dentate gyrus.
Many studies on the changes in the rhythm of nature (animate and inanimate) revealed that plants are good indicators of climate change, because they are responsive to air temperature variations. There is a clear trend towards earlier onset of plant development in Poland, especially for spring phenological events as flowering and leafing. The main objective of this study was to identify phenological tendencies of selected plant indicator species in Poland and to explore the statistical relationship between mean phenological onset date against mean monthly air temperature. Phenological observations of 9 plant species: Corylus avellana L., Tussilago farfara L., Padus avium L., Taraxacum officinale F.H. Wiggers coll., Betula pendula Roth, Syringa vulgaris L., Aesculus hippocastanum L., Robinia pseudoacacia L., Tilia cordata Mill. from 25 locations acquired in the period 1951–1990 were used. The results proved that phenological changes were the reaction of plants to air temperature variations. For spring and summer phenophases, the mean correlation coefficient was negative — higher air temperature was related to earlier onset dates. The advance of spring and summer phenology was estimated at -2.2 days per 1°C. Majority of phenological events were the most significantly correlated with mean monthly air temperature of the preceding months. Phenological trends observed in Poland were mainly negative (73% of analysed stations), significant (22%) but diversified between the selected species. Linear regression analysis of the onset dates against year (including regression coefficient) showed the highest values for Corylus avellana flowering: -0.24 days per year (20% significant) and Betula pendula leaf unfolding: -0.18 days per year (33% significant). Robinia pseudoacacia showed significant advances (72% of negative trends were significant) in a summer season with an average rate of -0.3 days per year. Whereas in case of Tilia cordata no tendencies to advance has been proved.
INTRODUCTION: In the recent years, more attention is attributed to the impact of diet on central nervous system function. An increasing number of diseases, including neurological disorders, results from inadequate dietary habits. Diet, however, can affect the function of brain and mental processes in a negative as well as a beneficial way. The diet with well‑documented neuroprotective effects is a high-fat and low-carbohydrate ketogenic diet (KD). METHOD(S): We investigated two type of KD, one of them based on animal (KDA), while the other on vegetable fats (KDB). Both diets were applied to two groups of laboratory animals: mice (129S2/SvPasCrl) and rats (Long‑Evans Rat, Crl: LE) for 6 weeks. CONCLUSIONS: Interestingly, preliminary data indicate a unique anxiogenic action of the KD but only in mice.
INTRODUCTION: Autophagy is a cellular recycling mechanism essential for maintenance of cell homeostasis and viability, especially during stress conditions; hence, autophagy is involved in a number of physiological and pathological processes. Autophagy is thought to be involved in anti‑aging and neuroprotective effects of caloric restriction, Sirtuin 1 activation, inhibition of insulin/ insulin-like growth factor signaling, and administration of rapamycin, resveratrol, and metformin. The ketogenic diet mimics the biochemical actions of fasting and exerts many physiological and cellular responses similar to those evoked by intermittent energy restriction. Despite this, the relationship between nutritional ketosis and autophagy has been a largely unexplored field. AIM(S): The aim of this study was to verify the hypothesis that ketogenic diets affect the process of autophagosome formation in the hippocampus and/or cerebral cortex. METHOD(S): 9-week-old male mice were fed with one of two differently composed ketogenic chows – based on the fat of either animal or plant origin (KA, KP respectively) or with standard rodent chow (SD) – for 6 subsequent weeks. Western blotting, (LC3, p62), QRT‑PCR (LC3A, LC3B, p62), and confocal microscopy (LC3 puncta) were employed to monitor autophagy in hippocampal and cerebrocortical samples. RESULTS: Western blot results revealed increased levels of LC3 II protein – a marker of autophagosomes – in the hippocampus and frontal cortex of mice treated with the ketogenic diet. This observation was confirmed by the evaluation of a number of LC3 puncta with immunofluorescence microscopy. The size of this effect was dependent on the composition of the diet. CONCLUSIONS: This study reports, for the first time, an upregulation of autophagosome synthesis in the brain of animals fed with the ketogenic diet. Our results make a significant contribution to the understanding of the mechanisms of ketogenic diet action. FINANCIAL SUPPORT: This research is supported by the National Science Center grant no. 2017/01/X/ NZ3/00984.
In the pathogenesis of central nervous system (CNS) disorders, an increasingly important role is attributed recently to unhealthy lifestyle, which consists primarily of a high caloric diet (i.e., western), chronic exposure to stress, and lack of physical activity. However, the mechanisms responsible for energy metabolism impairment induced by unhealthy lifestyles compromising CNS functions are poorly understood. Research on the effects of physical activity on the CNS is especially important, because it may result in the development of new methods of therapy inspired by natural protective mechanisms. In our study we employed a new and rarely used approach – a forced running wheel. The lack of electrical stimulus in the aforementioned system successfully makes a breakthrough in the study of animal physical activity. Physiological and behavioral responses of the organism to stress are closely related to sex. Epidemiological studies indicate that women are more vulnerable to the adverse effects of stress and despite that, most of the experimental studies are conveyed on male animals. The investigations were carried on female rats. The main goal of our study was to verify the hypothesis that regular exercise may reduce the disturbances induced by lifestyle modifications, like western diet and/or stress exposure. Adult female rats were fed with the prepared chow reproducing the human western diet and/or subjected to a stress induced by social instability. This stress protocol is characterized by a low degree of invasiveness. To evaluate if regular physical activity may reduce the adverse effects caused by diet and stress, female rats were additionally subjected to the procedure of forced physical activity. A proteomic analysis was conducted on samples obtained from the frontal cortex – a region that plays an important role in cognitive processes as well as is involved in the mechanisms engaged in the response to stress.
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