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1

Adaptation of wheat cells to short-term ozone stress: the impact of α-tocopherol and gallic acid on natural and model membranes

100%
Rudolphi-Skorska E., Sieprawska A.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 04
2

Physicochemical techniques in description of interactions in model and native plant membranes under stressful conditions and in physiological processes

100%
Rudolphi-Skorska E., Sieprawska A.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01
3

Physiological effects of toxic doses of manganese on wheat development

81%
Sieprawska A., Filek M., Tobiasz A.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
4

Electrophysiological and structural properties of natural and model membranes as a tool for measurement of lipids/polyamines interaction

81%
Filek M., Rudolphi-Skorska E., Sieprawska A., Zembala M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
5

The influence of selenium on the accumulation of K and Ca ions in rape seedlings subjected to cadmium stress

81%
Ploskonka J., Rudolphi-Skorska E., Sieprawska A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 545
The aim of the experiment was to study the effects of selenium ions on the changes in membrane permeability for K and Ca in the leaves of rape during cadmium stress. The of stress level was determined by changes in the mass increment and lipid peroxidation (MDA concentration) in 3-week-old seedlings cultured in vitro on Murashige and Skoog [1962] medium (control) and on medium containing 600 µМ CdCl2. Selenium was added at the concentration of 2 µМ (Na2SeO4) to the control and medium containing cadmium ions. The level of K and Ca was determined with ionoselective electrodes after one hour of shaking leaf discs (membrane permeability) and heating to 100°C (total ion content). Cadmium present in the nutrient medium inhibited the mass increment and increased MDA concentration, and significantly decreased membrane permeability for K and Ca. In the mixtures of cadmium and selenium, selenium ions partially reduced the effects of the stress-inducing cadmium action on the studied physiological parameters and membrane permeability to the studied ions. The observed protective effect of selenium can be the result of the effect of this substance on both the activity of ion channels and peroxidation of membrane lipids.
6

Electron paramagnetic resonance (EPR) spectroscopy in the investigation of oxidative stress in plants

81%
Labanowska M., Filek M., Kurdziel M., Sieprawska A.
Current Topics in Biophysics. Supplement
|
2013
|
tom 36
|
nr 2A: Posters
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Involvement of selenium in protective mechanisms of plants under environmental stress conditions-review

81%
Sieprawska A., Kornas A., Filek M.
Acta Biologica Cracoviensia. Series Botanica
|
2015
|
tom 57
|
nr 1
In recent years there has been growing interest in selenium (Se) as an important micronutrient not only for animals and humans but also for plants. In particular, its protective effect in plants exposed to stress conditions has been suggested. In spite of many studies, the mechanism of Se action is not fully understood. In this review, possible ways of interaction of Se with stress factors leading to optimal growth and development of plants are presented. As the majority of experiments have focused on the effects of Se application under stress conditions induced by heavy metals, special attention is paid to the results obtained in such studies. Changes of physiological and biochemical properties of plant cells, with particular regard to the influence of Se on the activation of enzymatic and non-enzymatic antioxidants under this stress, are summarized. Experiments in which Se was used in some other environmental stresses (drought, UV, cold and high temperature) are also cited. On the basis of the presented literature it is suggested that a positive effect of Se depends on both its doses and on chosen plant genotypes and is mainly connected with activation of antioxidative defense in plant cells.
8

Antioxidative action of Alpha-tocopherol in ozone stress of varying intensity

81%
Rudolphi-Skorska E., Sieprawska A., Filek M., Zembala M.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
9

Anatomic nad biochemical changes of wheat seedlings in response to the excess of manganese

81%
Sieprawska A., Filek M., Bednarska-Kozakiewicz E., Tobiasz A.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 11-11th International Conference Plant Functioning under Environmental Stress...
10

The influence of selenium compounds on the permeability of wheat callus cell plasma membranes in the presence of cadmium ions

81%
Wrona M., Sieprawska A., Tejchman W., Filek M.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Biologically active substances in plant extracts from mistletoe Viscum album and trees: fir (Abies alba Mill.), pine (Pinus sylvestris L.) and yew (Taxus baccata L.)

81%
Barbasz A., Kreczmer B., Rudolphi-Skorska E., Sieprawska A.
Herba Polonica
|
2012
|
tom 58
|
nr 1
Taking into account the growing number of reports which confirm the medicinal effects of preparations of plant origin, the paper analyzed the biochemical composition of extracts obtained from mistletoe parasitizing on fir and extracts from fir, pine and yew trees was analyzed. The mistletoe samples were collected in winter, spring, summer and autumn. Mistletoe’s extract is a source of viscotoxines – substances used in a treatment of heart diseases. Yew is also used as a plant which tissues contain substances of high therapeutic value. Extracts obtained from tissues of this plant are used for cancer treatment. The content of protein, soluble sugars and proline were determined in all samples. The biological activity of mistletoe may be connected with high concentrations of almost all investigated metabolites. The level of these compounds were higher than that of extracts from trees. An increased level of proline and malondialdehyde in the tissues of spring mistletoe is probably related to summary effect of environmental stresses in this season (temperature, insolation intensity and duration). Among polyamines, the highest concentration of spermidine in extracts from mistletoe and of spermine in extracts from trees may be a sign of specific metabolism pathway in investigated plants. Among extracts from trees tissues, fir was the richest in studied bioactive substances. The less amounts of all analyzed substances were found in tissues of pine.
12

Electron paramagnetic resonance (EPR) spectroscopy studies of the mycotoxin-stimulation of radical species in wheat

81%
Filek M., Labanowska M., Kurdziel M., Sieprawska A.
Current Topics in Biophysics. Supplement
|
2016
|
tom 39
|
nr A: Posters
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Content of antioxidantes in extracts of mistletoe (Viscum album L.), yew (Taxus baccata L.), pine (Pinus sylvestris L.) and fir (Abies alba Mill.)

71%
Barbasz A., Kreczmer B., Rudolphi-Skorska E., Sieprawska A., Woznica D.
Herba Polonica
|
2012
|
tom 58
|
nr 1
Imbalance between the intensity of oxidative processes (that induce the formation of reactive oxygen species) and counteracting antioxidant system is called oxidative stress. Most of the pathological changes in living organisms is associated with the processes of carcinogenesis induced by free radicals. State of equilibrium is maintained due to the presence of antioxidant enzymes (e.g. superoxide dismutase, peroxidase) and other biologically active substances such as glutathione, ascorbic acid and beta-carotene. These compounds enable the removal of reactive oxygen species in cells. The purpose of this study was to investigate the oxidative activity of mistletoe extracts and their potential hosts: fir and pine, and yew trees, which also have therapeutic properties. The results of performed analysis of enzymatic antioxidants (superoxide dismutase and peroxidase) lead to the conclusion that their activity in the tissues of mistletoe is much lower than in the tissues of fir, pine and yew. It was found, however, a much higher content of non-enzymatic antioxidants such as ascorbic acid, glutathione or beta- carotene in the tissues of mistletoe compared to other plants analyzed. Thus, extracts from mistletoe are a rich source of antioxidants easily assimilated to organisms receiving them.
14

Response of chloroplasts of tolerant and sensitive wheat genotypes to manganese excess: structural and biochemical properties

61%
Sieprawska A., Filek M., Tobiasz A., Bednarska-Kozakiewicz E., Walas S., Dudek-Adamska D., Grygo-Szymanko E.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 01
15

Trace elements’ uptake and antioxidant response to excess of manganese in in vitro cells of sensitive and tolerant wheat

61%
Sieprawska A., Filek M., Tobiasz A., Walas S., Dudek-Adamska D., Grygo-Szymanko E.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 02
16

Does micro- and macroelement content differentiate grains of sensitive and tolerant wheat varieties?

61%
Sieprawska A., Filek M., Walas S., Tobiasz A., Mrowiec H., Miszalski Z.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 11
Environmental stresses are forcing breeders to produce new plant genotypes with higher resistance to stressors. Biochemical markers of stress tolerance would assist in the selection of tolerant cultivars on the early stages of plant development. The aim of these studies was to examine whether the concentration of micro and macroelements of embryos and/or endosperm could specify the wheat grains in terms of their tolerance to stress conditions. Two sensitive to drought (Radunia and Raweta), two tolerant (Nawra and Parabola) and one with intermediate tolerance (Manu) were chosen. After dividing embryos and endosperm, the microelements content (Mn, Fe, Cu, Zn and Mo) was analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and macroelements (K, Ca, Mg, P and S) by inductively coupled plasma optical emission spectrometry (ICP-OES). Independent of genotype, the concentration of all elements was higher in embryos than in endosperm. In both embryos and endosperm of tolerant plants, higher content of microelements (except for Cu in embryos) was detected. The accumulation of macroelements was lower in embryos of tolerant plants (except for K), however, in the case of endosperm, higher amounts of these elements, were registered. In embryos of Manu genotype, the content of microelements was more alike to sensitive and macroelements to tolerant plants but in endosperm, the level of both micro- and macroelements was more similar to tolerant ones. It was concluded that mineral composition of wheat grains, especially those in embryos, could inform about possible resistance of genotypes to stress conditions.
17

Membrane permeability and micro- and macroelement accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress

61%
Filek M., Walas S., Mrowiec H., Rudolphy-Skorska E., Sieprawska A., Biesaga-Koscielniak J.
Acta Physiologiae Plantarum
|
2012
|
tom 34
|
nr 3
The comparative responses of ten spring wheat cultivars to water stress were investigated. Wheat plants were cultured under hydroponics conditions (Hoagland nutrient) to the stage of three-leaf seedlings. Then, the water medium was supplemented with PEG (drought) or NaCl (salinity) to obtain a water status equal to -1.5 MPa. After a 2-day treatment, the changes in the following parameters were determined: fresh and dry weight, macroand microelement accumulation, membrane injury (electrolyte leakage, lipid peroxidation) and fatty acid content of the phospholipid fraction of plasmalemma (in comparison to plants not stressed, taken as a control). Generally, the plants were more significantly influenced by water stress stimulated by PEG than by NaCl treatment, as compared to the plants cultivated in the control media. The results of the decrease in water content in leaves and electrolyte leakage from cells corresponded well with the intensity of lipid peroxidation (determined by malondialdehyde—MDAcontent) and were chosen for the selection of investigated genotypes for tolerance to both stresses. The more tolerant genotypes exhibited the opposite changes in phospholipid fatty acid unsaturation for two applied stresses i.e. NaCl treatment caused a decrease in unsaturation whereas in PEG-treated plants an increase in unsaturation was observed. These changes were reversed for less tolerant plants, i.e. NaCl treatment influenced an increase in fatty acid unsaturation whereas in PEG-treated plants a decrease in unsaturation was measured. The ratio of U/S (unsaturated to saturated fatty acids) correlated with the total amount of accumulated macroelements. The content of Mg, Ca and S in leaves of plants undergoing both stress factors (NaCl and PEG) dropped whereas the K and P content increased in leaves of wheat seedlings cultured on media containing NaCl only. For microelements, a decrease in the accumulation of these nutrients was detected in all investigated seedlings. However, a greater reduction in the level of these elements occurred in seedlings grown on media with PEG in comparison to those grown on NaCl containing media.
18

Cultured endosperm versus endosperm in bread wheat caryopsis: biochemical, ultrastructural and molecular studies

51%
Popielarska-Konieczna M., Kleszcz I., Kwolek D., Marcinska I., Juhasz A., Makai S., Filek M., Sieprawska A., Kozieradzka-Kiszkurno M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2016
|
tom 58
|
nr 1
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