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Allelopathy is defined as mechanism of plant-plant, plant-microorganisms, plant-virus, plant-insect, and plant-soil-plant interactions mediated by plant- or microorganism-produced chemicals released to the environment. The majority of allelochemicals are secondary metabolites and among others belong to terpenoids, phenolic compounds, organic cyanides and longchain fatty acids. The action of allelochemicals in target plant is diverse and affects a large number of biochemical reactions resulting in modifications of different physiological functions. Thus the results of allelochemical action can be detected at different levels of plant organization: molecular, structural, biochemical, physiological and ecological. Enzyme activities, cell division and ultrastructure, membrane permeability, ion uptake and as a consequence plant growth and development are modified by allelochemicals. Significant effects on photosynthesis and respiration are the best-characterized results of allelopathic interactions. Moreover allelopathic compounds seem to induce a secondary oxidative stress expressed as enhanced free radical production and induction of cellular antioxidant system. Plant survival under allelopathy stress conditions depends on plant defense leading to allelochemical detoxication, the process which may go on in parallel to cell defense reaction to oxidative stress. The article presents some aspects of the current knowledge regarding mechanisms of the allelopathy phenomenon. The allelopathy is a complex problem, thus comprehensive understanding of allelochemical mode of action requires further investigation and still remains an open question.
Nitric oxide (NO) and cyanide (HCN) are small gaseous molecules that have been intensively studied to explain their role in plant development, metabolism and reaction to stresses. Cyanide and NO are known to be produced endogenously during early phase of seed germination or are present in the environment. Both molecules regulate breakage of seed dormancy and accelerate seed germination. Regulatory role of cyanide in breaking of dormancy seems to be understood to some extend, while the NO mode of action is much less explained. However, some similarities could be suggested. The mechanisms involved in HCN-dependent dormancy breakage in apple embryos are summarized in relation to NO-donor mediated stimulation of germination.
Recent biochemical and genetic studies on hydrogen cyanide (HCN) metabolism and function in plants were reviewed. The potential sources of endogenous cyanide and the pathways of its detoxification are outlined and the possible signaling routes by which cyanide exerts its physiological effects are discussed. Cyanide is produced in plant tissues as the result of hydrolysis of cyanogenic compounds and is also released as a co-product of ethylene biosynthesis. Most cyanide produced in plants is detoxified primarily by the key enzyme P-cyanoalanine synthase. The remaining HCN at non-toxic concentration may play a role of signaling molecule involved in the control of some metabolic processes in plants. So, HCN may play a dual role in plants, depending on its concentration. It may be used in defense against herbivores at high toxic concentration and may have a regulatory function at lower concentration. Special attention is given to the action of HCN during biotic and abiotic stresses, nitrate assimilation and seed germination. Intracellular signaling responses to HCN involve enhancement of reactive oxygen species (ROS) generation and the expression of cyanide-insensitive alternative oxidase (AOX) and ACC synthase (ACS) genes. The biochemical and cellular mechanisms of these responses are, however, not completely understood.
We examined the response of plants of various crop and weed species to cyanamide in order to evaluate allelochemical- mediated interactions between the species. We studied germination and seedling growth in the common weeds Galium aparine L. and Amaranthus retroflexus L., and the crops Zea mays L., Triticum aestivum L., Lactuca sativa L., Solanum lycopersicum L. and Sinapis alba L. as acceptor plants. Concentration-dependent phytotoxic effects of cyanamide were noted during seed germination and in the root and shoot growth of the tested plants. The monocotyledonous plants generally were less sensitive to cyanamide treatment. Seed germination and seedling growth of the dicotyledonous plants were strongly inhibited by the allelochemical at both tested concentrations (1.2 mM, 3 mM). We conclude that cyanamide has potential for use as a natural herbicide only in specific field systems of cyanamide-tolerant monocotyledonous crops accompanied by cyanamide-sensitive dicotyledonous weeds.
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