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1
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Induced mutagenesis in Cicer arietinum

100%
Mullainathan L., Umavathi S.
International Letters of Natural Sciences
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2014
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tom 07
The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea.
2
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Mutagenic effect of gamma rays and EMS on seed germination, seedling height reduction and survivability of chick pea (Cicer arietinum L.) var. Co - 4

100%
Umavathi S., Mullainathan L.
International Letters of Natural Sciences
|
2014
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tom 11
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nr 1
The effect of gamma irradiation and EMS treatment on seed germination and seedling height of Chick pea (Cicer arietinum. L.). In this regard Co-4 variety of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50, and 60 kR) and EMS (10, 20, 30, 40 and 50 mM) for inducing mutation. The effect of mutagen was observed on the basis of percentage of seed germination, seedling height reduction at 15th day and survivability. From the result it was observed that, the percentage of seed germination, seedling height reduction at 15th day and survivability were significantly decreased with increasing doses/concentrations of mutagen. The effective doses/ concentrations which caused 50 % growth reduction were 40kR in gamma rays and 30 mM in EMS.
3
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Effect of gamma rays in induced morphological mutants on M2 generation of chilli (Capsicum annuum L.) var K1

100%
Mullainathan L., Aruldoss T.
International Letters of Natural Sciences
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2015
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tom 03
The present study was carried out to induce morphological mutation in chilli (Capsicum annuum L.) var k1. The seeds were subjected to different treatment levels of gamma rays are 20, 30, 40, 50, 60 and 70Kr along with control. The treated and untreated seeds were raised in a randomized block design to observe the different morphological characters in M2 generation. Several unique and interesting mutants were isolated in this investigation, these independent mutants have differ from control, the most distinct mutants included tall, dwarf, bushy , flower, sterile and pod mutant, was observed in M2 generation.
4
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Physical and chemical induced mutagenesis study for identifying lethality dose in chick pea (Cicer arietinum L.) Var. Co - 4

100%
Umavathi S., Mullainathan L.
International Letters of Natural Sciences
|
2015
|
tom 08
The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.
5
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Studies on effect of physical and chemical mutagens in sorghum (Sorghum bicolor (L.) Moench) in M2 generation

100%
Suthakar V., Mullainathan L.
International Letters of Natural Sciences
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2015
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tom 37
Seeds of Sorghum (sorghum bicolor (L.) Moench) Variety CSV – 23. Were mutagenesis with gamma rays and EMS to determine their mutagenic sensitivity in morphological and yield characters. The spectrum of Mutation and induced variability for various quantitative and yield traits were observed in M2 generation. Plant height (cm),total number of leaves, leaf length (cm), leaf width(cm), stem girth (cm),Days taken to first flowering (days),ear head length(cm),ear head width(cm), Grain weight(gm),100 seed weight(gm), Biomass weight(gm).
6
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The effect of ethyl methane sulphonate and diethyl sulphate on chilli (Capsicum annuum L.) in M1 generation

81%
Sanjai Gandhi E., Sri Devi A., Mullainathan L.
International Letters of Natural Sciences
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2014
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tom 05
In recent years, the demand of chilli has tremendously increased due to its attractive market price and multifarious used in cooked and processed forms. At present people are much concerned about the fruit quality and yield. Therefore, attention is being paid for development of genotypes having high yield potential with desirable fruit quality characters in a short period of time. For this purpose, seeds of chilli were mutagenised with ethyl methane sulphonate (EMS) and diethyl sulphate (DES) to determine their mutagenic sensitivity in M1 generation. The increasing concentration of EMS and DES decreased in morphological and yield characters. The spectrum of mutation and induced variability for various quantitative traits were observed in M1 generation such as germination (%), plant height, primary and secondary branches per plant, days to first flowering, fruit length (cm), fruit girth (cm), total number of fruits per plant, number of seeds per fruit, seed weight per fruit (g), 100 seed weight (g) and pericarp: seed ratio showed variability in chilli with the effect of EMS and DES. The percentage of chromosomal abnormalities in different mitotic stages was significantly higher than that of the control in all the treatment concentrations.
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Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker

81%
Mullainathan L., Sridevi A., Umavathi S., Sanjai Gandhi E.
International Letters of Natural Sciences
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2014
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tom 06
The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
8
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Cytological studies in cluster bean by the application of physical and chemical mutagens (Cyamopsis tetragonoloba L.)

81%
Mullainathan L., Aruldoss T., Velu S.
International Letters of Natural Sciences
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2014
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tom 16
The miotic and meotic behaviour is considered to be one of the most dependable indices to estimate the potency of mutagen cytological aberrations in plants serve as an excellent monitoring system for the determination of mutagens that may cause a genetic hazard in the present study cluster bean contains 7 bivalents (24 = 14) for the present investigation the plant exposed to dolf concentration of 60, 80, and 100kr gamma rays and 0.4, 0.6 and 0.8 % Mm ( EMS ) the present study reveals various chromosomal aberration includes stickiness precocisus movement ,single and anaphasis bridge laggards and synchronized movement of chromosomes at anaphase.
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