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1
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Insecticidal efficacy of silica nanoparticles against Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val

100%
Ziaee M., Ganji Z.
Journal of Plant Protection Research
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2016
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tom 56
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nr 3
Bioassays were conducted to assess the effects of two silicon dioxide nanoparticles of Aerosil® and Nanosav against adults of Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val. Silica nanoparticles were applied at the rates of 50, 100, 200 and 300 mg · kg–1 on wheat and peeled barley. The mortality was counted after 1, 2, 3, and 7 days of exposure. Another experiment was carried out to evaluate the effect of food source on the survival of beetles after exposure to silica nanoparticles. Adults were exposed to silica nanoparticles at the rate of 0.2 mg · cm–2 for 1 and 2 days on filter paper inside plastic Petri dishes, respectively. After exposure, the initial mortality was counted and live individuals of both species were held for a week in empty glass vials or vials containing wheat and wheat flour, respectively. Silica nanoparticles have high toxicity on R. dominica and T. confusum adults. Rhyzopertha dominica was more susceptible than T. confusum. However, the mortality of both species increased with increasing concentrations and time exposed to each concentration. At low concentrations, Aerosil® was more effective than Nanosav. Silica nanoparticles were more effective in wheat grains than barley. Results indicated that the initial mortality was so high that the impact of food source on delay mortality was unclear in most cases. Silica nanoparticles were efficient against tested species and can be used effectively in a stored grain integrated pest management program.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Diatomaceous earth used against insect pests, applied alone or in combination with Metarhizium anisopliae and Beauveria bassiana

80%
Shafighi Y., Ziaee M., Ghosta Y.
Journal of Plant Protection Research
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2014
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tom 54
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nr 1
Laboratory bioassays were conducted to assess the insecticidal efficacy of the formulation SilicoSec® used alone or in combination with isolates of entomopathogenic fungi, Metarhizium anisopliae (Metschinkoff) Sorokin and Beauveria bassiana (Balsamo) Vuillemin. SilicoSec® is a commercial diatomaceous earth (DE) formulation. Wheat was treated with 200 mg/kg of DE, 400 mg/kg of each isolates alone or a combination of them, against Tribolium castaneum Herbst, Rhyzopertha dominica (F.), and Oryzaephilus surinamensis L. The experiments were carried out at 27±1°C and 65±5% relative humidity (RH) in continuous darkness. The pathogenicity of all isolates was significantly low even after 7 days of exposure, with the exception of R. dominica. The isolates were virulent to the beetles, but the efficacy of the isolates was enhanced in combination with the DE. Tribolium castaneum was the most resistant species, followed by R. dominica. The findings indicated that the addition of the DE to the isolates increased the pathogenicity especially at the highest exposure interval. The addition of DE may provide satisfactory control of the insect-pests of stored products.
3

Control of pulse beetle, Callosubruchus maculatus (F.) (Coleoptera: Bruchidae) in different cereals using spinosad dust in storage conditions

80%
Khashaveh A., Ziaee M., Safaralizadeh M. H.
Journal of Plant Protection Research
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2011
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tom 51
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nr 1
Effectiveness of spinosad dust formulation, that contains 0.125% spinosad, was evaluated against adults Callosubruchus maculatus (F.) on four commodities: chickpea, split pea, cowpea and lentil. Spinosad was applied at three dose rates: 0.1, 0.2 and 0.3 g/kg, corresponding to 0.125, 0.25 and 0.375 mg/kg of the active ingredient, respectively. The experiment was carried out at 27°C and 55±5% relative humidity. Adults mortality was measured after 1, 2, 5 and 10-days of exposure. After the 10-day mortality count, all surviving insects were removed and samples retained under the same conditions for a further 35 days to assess progeny reduction. Mortality of exposed individuals in all treated commodities was low at 1-day exposure even at 0.3 g/kg and did not exceed 20%. As expected, mortality increased with the increase of exposure interval and dose rates. A significant difference was observed among the four commodities. After 10 days of exposure, mortality reached 100% in all commodities except for split pea. The application of spinosad significantly reduced progeny production in four commodities tested in comparison with the untreated ones. High reduction in progeny production was recorded when spinosad was applied at the rate of 0.3 g/kg on split pea and cowpea (94.33 and 94.21%, respectively). The results of our study clearly revealed that spinosad dust could be successfully used as a grain protectant against C. maculatus. Further experimentations still need to be done to examine higher dose rates and long-term use in different commodities.
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