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The aim of the research was to analyse the weaknesses of the pH differential method for strawberry anthocyanin determination. The work is based on practical experiments with 12 strawberry cultivars and on analysis of published papers. We used following molar absorption coefficients ( ƹ values): 26900 and 29600 M⁻¹ cm⁻¹ for cyanidin 3 glycoside (C3g) and 15600, 22400, 27300 and 36000 M⁻¹ cm⁻¹ for pelargonidin 3 glycoside (P3g). In order to show how the calculated value of total anthocyanins depends on the predominant anthocyanin used for the calculations, we compared the results of spectroscopic and chromatographic analysis. Present research demonstrated that different ƹ values may influence the results of total anthocyanins even more than cultivar properties. The most frequently used ƹ values 26900 M⁻¹ cm⁻¹ and 29600 M⁻¹ cm⁻¹ gave underestimated values. C3g was present in minor amounts in all cultivars. Conclusively, P3g with the ƹ = 15600 M⁻¹ cm⁻¹ should be used for ensuring most precise estimation of total anthocyanin content in strawberries.
The aim of the experiment was to determine the effect of two storage tempera­tures (+1 °C and +4 °C) and modified atmosphere packaging (MAP) on the posthar- vest quality of 'Glen Ample' raspberry. Fruits were picked into 450-g well-ventilated plastic punnets, cooled down for 24 hours and packed as follows: control - unpacked ventilated punnets covered with a lid; punnets wrapped in Xtend® film, punnets wrapped with PP30 - 30 ^m polypropylene film and punnets wrapped with OPP25 - 25 ^m oriented polypropylene film. One treatment consisted of eight replicate bags. After 3 days in cool storage, all packages were kept at +15 °C for 24 hours to simulate retail conditions. The O2 and CO2 content in the packages was measured daily. Soluble solids con­tent (SSC), titratable acidity (TA), fruit firmness, ascorbic acid content (AAC), antho- cyanins (ACY) and total antioxidant capacity (TAC) were determined at harvest and at the end of storage. The number of spoiled fruits was recorded and a sensory evalua­tion was carried out at the end of the experiment. Vol. 19(1) 2011: 145-153 During the first 24 hours, the concentration of CO2 increased to 10% in the PP30 and OPP25 packages at +4 °C, while at +1 °C it took 48 hours to reach that level. In the Xtend® packages, CO2 concentration increased above 10% only after the shelf life simulation. The environment in the PP30 and OPP25 packages became anaerobic in retail conditions. Fruit spoilage was significantly lower in the PP30 and OPP25 pack­ages, where the CO2 content increased rapidly. Raspberries had higher SSC, TA, SSC/TA and ACY at +4 °C than at +1 °C. Fruits in the Xtend® packages were significantly firmer than in the other treatments. The mean effect of MAP and the storage temperatures was such that they had no significant influence on raspberry fruit appearance, flavour and off- flavour. Raspberries kept at +4 °C tasted better to the evaluators.
The aim of the research was to find out how foliar selenium (Se) treatment in open field conditions affects plant nutrition, yield and bulb bioactive properties. 10, 50 or 100 μg·mL⁻¹ Se, referred to as Se10, Se50 and Se100 treatments, was applied to onion ‘Hercules’ in 2008 and 2009. Bulb weight, total yield, content of total Se, free selenomethionine and selenomethylselenocysteine, total S, N, P, K, Ca and Mg, content of total phenolics, pungency and total antioxidant capacity (TAC) were determined. All Se treatments significantly reduced bulb S content. The Se100 treatment had a tendency to decrease bulb size and yield. The Se50 treatment increased total Se content, total phenolics and TAC and had a tendency to increase the yield. A larger proportion of total Se was converted into organic compounds in the Se50 than in the Se100 treatment. In Se 10 treatment, bulb TAC was the highest among treatments in 2009. Considering both agronomic and human health benefits, Na2SeO4 solution at the rate of 50 ȝg·mL⁻¹ Se can be recommended for bulb onion.
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