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The aim of our study was to examine the efficacy of various disinfectants originally recommended for use in the food industry or medicine, in reducing bacteria Clavibacter michiganensis ssp. sepedonicus. Disinfecting preparations: Pro-Tech, Bakterierent, Bacto Detsan, Mexept, Diesis Forte, Apesin AP 300 were tested taking into account various parameters such as concentration of working solution, environment temperature and duration of treatment. Studies showed, that all tested substances were effective in reducing the growth of bacteria C. michiganensis ssp. sepedonicus in concentrations recommended by the manufacturers (both at 4°C and 20°C), only if duration of procedure was no shorter than 15 minutes. For Mexept, Diesis Forte and Apesin AP 300 shorter disinfection time did not affect the efficacy in reducing the growth of bacteria Cms. Other experiments included the impact of metal surfaces on the effectiveness of selected disinfectants. Bactericidal activity against bacteria C. michiganensis ssp. sepedonicus showed: Bakterierent 2%, Bacto Detsan 1%, Mexept 1%, Apesin AP 300 2%, Pro-Tech 0.5%, Diesis Forte 0.5% (sometimes concentrations active against Cms were higher than those recommended by the manufacturer of the preparation). A prolonged for 15 minutes disinfection procedure was sufficient to obtain a bactericidal effect. As a result of the chosen method of disinfection the type of different treated surfaces such as metal, steel, wood or concrete did not affect significantly the effectiveness of antimicrobial preparations with exception of Mexept and Pro-Tech.
The aim of the performed research was to investigate the occurrence of bacterial diseases of maize in Poland and also a characterization and diagnostics of maize pathogenic bacterial species. Using biological, biochemical, serological and molecular methods a collection of nearly 200 isolates was analyzed. It was found that bacterial isolates belonged to the following genera: Erwinia, Pantoea, Xanthomonas, Xylophilus, Acidovorax, Enterobacter, Pseudomonas i Bacillus. By all used diagnostics methods the presence of 4 isolates of Pantoea ananatis species was confirmed. This species is well known bacterial plant pathogen of maize and many other plants. However, its presence was recorded in Poland for the first time. A bacterial population naturally existing together with plant pathogenic Pantoea ananatis was also characterized.
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