Wyniki wyszukiwania

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Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

100%

Xia Y., Lv Y., Yuan Y., Wang G., Chen Y., Zhang H., Shen Z.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 5

EhMT1, a type 1 metallothionein (MT) gene, was cloned from Elsholtzia haichowensis, a Cu-tolerant plant. Typical of plant type 1MTs,EhMT1encodes a putative peptide of 74 amino acid residues containing cysteine-rich domains. Sequence comparisons with various databases revealed strong similarities at the nucleotide and amino acid levels between EhMT1 and the type 1 MT of Mimulus guttatus. EhMT1 transcriptionwas greater in the roots than in the leaves, and was markedly increased by treatments with Cu, H₂O₂, and heat shock. EhMT1-GUS was localized to the cytoplasm of onion epidermal cells. Escherichia coli cells expressing pET-30a-EhMT1 were more tolerant to Cu and accumulated more Cu than control cells. Our results show that EhMT1 is involved in Cu tolerance and accumulation in E. haichowensis.

2

All-region human health risk assessment of Cr(VI) in a coal chemical plant based on Kriging

100%

Zhang K., Yang J., Wang Y., Xia Y., Liu S., Zhang R.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 1

3

Analysis of heavy metal sources and health risk assessment of typical coal mine collapsed lakes in Huaibei Coalfield, Anhui Province, China

84%

Li J., Gui H., Hu R., Chen L., Xia Y., Fan H., Yu H., Wang M.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 5

4

Downregulation of the lycopene ε-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum

60%

Shi J., Liu P., Xia Y., Wei P., Li W., Zhang W., Chen X., Cao P., Xu Y., Jin L., Li F., Luo Z., Wei C., Zhang J., Xie X., Qu L., Yang J., Lin F., Wang R.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 10

Lycopene ε-cyclase (ε-LCY) functions at a branch point of the carotenoid biosynthesis pathway and modulates the ratio of lutein to the β-carotenoids. RNA interference (RNAi) and overexpression (OE) of Ntε-LCY were used to evaluate the physiological roles of ε-LCY in Nicotiana tobacum. In leaves, strong accumulation of β branch carotenoids and high expression of carotenoid biosynthesis genes resulted from suppression of Ntε-LCY expression. RNAi plants showed enhanced salt and drought tolerance, while overexpression of the Ntε-LCY gene weakened tolerance to salt and drought stress, as compared to control. Further analysis revealed that RNAi plants exhibited less water loss and had lower reactive oxygen species levels than did WT plants after both the salt and drought treatments. Further, higher levels of ABA accumulated in the RNAi lines than in the WT lines under stress conditions. These results suggest that reduced Ntε-LCY expression can improve drought and salinity tolerance in Nicotiana tabacum by enhancing their ROS scavenging ability.

Ograniczanie wyników

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

All-region human health risk assessment of Cr(VI) in a coal chemical plant based on Kriging

Analysis of heavy metal sources and health risk assessment of typical coal mine collapsed lakes in Huaibei Coalfield, Anhui Province, China

Downregulation of the lycopene ε-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum