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Five species of beans were chosen to observe the effects of intermittent periods and doses of cell phone electromagnetic field radiation (EMR) on germination seeds. Beans were grown under the same environment and the EMR was different. The treatment included two intermittent periods (4 and 24 h) and three radiation intensities (high, low, and check). The frequency of EMR was 1805-1850 MHz. The mean power density was 0.4809 mW/cm2 for high treatment and 1.455 mW/cm2 for low treatment. The specific absorption rating (SAR) values were measured and expressed as units of W/kg for the five beans. The effects of EMR on the germination rates of seeds were inconsistent. Germination differed under EMR treatment for red bean, soybean, and Mologa bean but not mung and Hyacinth bean. The 24 h intermittent exposure had a significant effect on the height and fresh weight of mung beans. Results differed under high, low, and with check. The 4 h intermittent exposure did not significantly affect the height or fresh weight. Hyacinth and mologa beans showed similar results. Only 24 h intermittent exposure with high treatment had a significant effect on height and fresh weight. Under the same EMR and exposure times the received dose was the same. However, bean species had different germination.
Tsunami disasters have frequently occurred in recent years. More and more researchers are focusing on this topic. To investi-gate the tsunami bore impact mechanism on a container model, a multi-functional slope-changing tsunami flume is built in this study. To simulate a tsunami bore, a dam-break wave was generated by a free-falling gate in a reservoir. A needle water level gauge and a high-speed camera were used to measure the tsunami wave heights and velocities for different storage water levels in the test flume, and the corresponding Froude numbers of tsunami waves were also calculated. The factors af-fecting the movement distance of the tsunami wave impacting the container model are explored in this experiment, and the results show that the movement distance is positively correlated with the storage water level, and negatively correlated with the container density and the coast slope
Recent physiological studies indicate that weightlessness reliably alters ocular structure and function, as well as the ability to process visual‑spatial information. The posterior parietal cortex (PPC) and lateral geniculate nucleus (LGN) are two key brain areas implicated in the processing of visual‑spatial information. Here, we used the modified tail‑suspension rat model to simulate the physiological effects of microgravity. Rats were divided into four groups, which exposed to the simulated microgravity environment for 0 (Control group), 7, 14, or 28 days. We found a significant increase in cellular apoptosis in the PPC and the LGN after 7 days of simulated microgravity. In addition, there was an increase in expression of c‑Fos protein in the PPC, and a repression of the PI3K/Akt signaling pathway in the LGN after 7 days. Based on these results, we conclude that short‑term simulated microgravity may induce cell apoptosis in the PPC and LGN, and reflect a neural adaptive process to accommodate a microgravity environment
Large scaled projects are conducted in South Yellow Sea in recent years. Topographic effect and tidal current are key issues to the coastal engineering and the ocean engineering. In this study, field surveys were conducted to investigate the tidal level, current velocity, and current direction in South Yellow Sea. A numerical model was developed to simulate the radial current field based on the field data. To investigate the mechanism of the radial current field, the actual topography and a smoothed topography were applied in the numerical model, respectively. Results show that, the current field appeares radial because of the tidal system rather than the submarine topography. Local topography centralized the radiation centre and shifted the high-velocity zones. The actual topographic effect is proposed, and results show that local topography increases the flood tide velocity and decreases the ebb tide velocity. Lagrangian residual currents are calculated to illustrate possible sediment sources and transport routes
It will be a new approach that BIM’s capital project lifecycle management (CPLM) applied to the yacht industry. This paper explored the feasibility of applying the principles and rationales of BIM for capital project lifecycle management in luxury yacht design, engineering, fabrication, construction and operation. The paper examined the premises and backbone technology of BIM. It then evaluated leading naval engineering and shipbuilding software applications and their development trends from the functional lens of BIM. To systematically investigate a BIM-based approach for capital project lifecycle management (CPLM) in the luxury yacht industry, the paper proposed and outlined an implementation framework. A case study and a student competition use case were discussed to delineate the core constituents and processes of the proposed framework. The case of BIM was reviewed. Through the domestic custom luxury yacht design and prototyping student competition, the application of this framework in educational research is demonstrated and the initial quantitative assessment of the framework is carried out. Conclusions: a BIM-based CPLM implementation framework can help the luxury yacht industry capitalize on the global transformation to an information-centric and data-driven new business paradigm in shipbuilding with integrated design, manufacturing and production
As energy crises and environmental pollution become increasingly prominent, people are beginning to explore the ocean to exploit its renewable energy. Based on hydrodynamic principles, an analytical model for unstable hydrofoil motion has been developed. The software ANSYS Fluent was employed to perform a simulation of hydrofoil motion, and the effect of hydrofoil motion on the surrounding flow field was analyzed. The hydrodynamic characteristics and energy-capturing efficiency of the hydrofoil were obtained, and the influence of complex flows on hydrofoil hydrodynamics was elucidated. The energy dissipation mechanism during hydrofoil motion was characterized. The results indicate that the vortex generation and shedding inevitably dissipates part of the tidal energy captured by the hydrofoil, which leads to abrupt changes in hydrofoil hydrodynamics and reduces energy-capturing efficiency. When the frequency of the abrupt hydrodynamics change matches the hydrofoil natural frequency, it may result in hydrofoil resonance and damage. Also, it is observed that larger pitch amplitude leads to larger optimalreduced frequency corresponding to the peak power cycle-averaged coefficient. The results also provide a theoretical guide on how to improve hydrofoil energy-capturing efficiency of the power generation system with control valves and extend hydrofoil life.
Safflower varieties were commonly divided into high, low and middle linoleic acid (LA) types according to their LA relative percentage contents in the seed oil. Fatty acid desaturase 2 (FAD2) plays a key role for LA content in seed. The sequence variations of FAD2 (CtFAD2-2, CtFAD2-10, CtFAD2-11) genes which could express in developmental seed of safflower were analyzed in 15 different LA-type materials. The results revealed that the CtFAD2-2 sequences were the same in all materials, and the CtFAD2-10, CtFAD2-11 sequences formed into two haplotypes independent of the LA-type of safflower seed. Yeast expression analysis revealed that two haplotypes of CtFAD2-10 had the function of oleic acid desaturase. The real-time PCR analysis of FAD2 in high and low LA-type materials at different seed developmental stages revealed that CtFAD2-2, CtFAD2-10 and CtFAD2-11 expressed mainly at 10 days after flower (DAF) for two different LA-type materials and the accumulation of few mRNA was detected in 14–22 DAF. The expression pattern of CtFAD2-1 in two different LA-type safflower materials was significantly divergent. For high-LA type, the accumulation of CtFAD2-1 mRNA was extremely low in 10 DAF, and its expression level increased sharply between 14 and 18 DAF and decreased slightly after 18 DAF. For low-LA type, the accumulation of CtFAD2-1 mRNA was extremely low during seed development stages. In conclusion, the gene structure and expression level of CtFAD2-1 may be the main factor affecting the differentiation of LA-type for safflower materials.
Phosphorus (P) is an essential macronutrient for plant growth and development; however, soil P available for plant absorption is often limited, putting constraints over agricultural sustainability. Understanding the physiological and molecular responses to P deficiency can help design strategies for diagnosis and mitigation of P deficiency in crop plants. The advent of the next-generation sequencing technologies has made it possible to characterize genome-wide molecular responses to P deficiency in plants. However, such research efforts are very limited for woody crops. In this study, RNA-seq was used to investigate P starvation-induced transcriptomic changes in roots of a frequently used citrus rootstock, Poncirus trifoliata (L.) Raf. A total of 1,135 genes showed differential expression in response to P deficiency. The transcriptomic data were further validated by real-time quantitative RT-PCR. Interestingly, at least one or more P-responsive cis-elements (P1BS) were found in the promoter regions of 76 differentially expressed genes. Functional annotation revealed that the predicted proteins of 117 of the differentially expressed genes were assigned to the categories of transporters, transcription factors or components involved in plant hormone signal regulation, suggesting that these genes may play important roles in response to P starvation. A comparative analysis of the citrus- and Arabidopsis-responsive transcripts under P deficiency also identified 174 commonly regulated genes, including those involved in P metabolism. Taken together, our transcriptomic data revealed changes of genome-wide gene expression in responses to P starvation in Poncirus, which should provide a solid basis for future identification and characterization of key genes involved in nutritional stress response in citrus rootstocks.
This study investigated heavy metals in soils and agricultural products near an industrial district in Dongguan City. The concentrations of Cu, Zn, Pb, Cd, Hg, and As in soil and agricultural products from vegetable and banana fields were determined. Results indicated that except for Zn in one sample and Cd in five samples, the majority of the samples were notably enriched by heavy metals compared with background values. The concentrations of heavy metals (mg·kg⁻¹ dry soil) in agricultural soils in the study area ranged from 22.2 to 93.0 for Cu, 31.2 to 213.6 for Zn, 47.6 to 133.5 for Pb, 0.01 to 0.67 for Cd, 0.15 to 0.56 for Hg, and 20.5 to 28.9 for As. Among these soil samples, concentrations of Cu, Zn, Cd, and Hg in 20.6%, 8.8%, 29.4% and 38.2% soil samples, respectively, exceeded Chinese maximum allowable concentrations (MAC) for agricultural soil. The concentrations of Zn, Pb, and Cd were significantly higher in vegetable than in banana fields. Cu, Zn, Pb, Cd, and Hg accumulated in the topsoil of vegetable fields, but only Pb and Hg accumulated in banana fields. Zn, Cd, and Hg accumulated more easily in flowering cabbage than other vegetables. Cu, Zn, and As were accumulated more easily in banana than lettuce, bunching onion and eggplant. Therefore, the findings suggest more attention should be focused on the accumulation of heavy metals in banana. This study presents a practical methodology for screening crops with lower bio-concentration factors for heavy metals to reduce metal contaminants in the general food supply chain.
Bio-oxidation of methane in a landfill environment is important for mitigating global methane emissions into the atmosphere. In the present study, the characteristics of methane bio-oxidation and methane-oxidizing microorganisms were studied by enrichment cultivation using fresh and aged leachate (collected from Qizishan Landfill, Suzhou, China). Both aerobic and anaerobic methane oxidation were detected, methane oxidation capacities of the culture liquid were 5.24–7.26 µmol/mL/d under aerobic conditions and 4.41-3.70 µmol/mL/d under anaerobic conditions. The stoichiometry of anaerobic oxidation of methane (AOM) showed the complexity of AOM mechanisms in the leachate culture, with the types of sulfate-dependent, denitrification-dependent and iron-dependent AOM. The 16S rRNA gene sequence analysis and SEM analysis results showed that the genus Methylocystis was the dominant bacteria in aerobic cultures (relative abundance 35.96–78.37%). Genus Moheibacter (41.38%) and Cupriavidus (43.08%) were the most dominant taxa in anaerobic cultures, with aerobic methanotrophs Methylocaldum and Methylocystis in low abundance, and no anaerobic methane-oxidizing archaea (ANME) was found. Further research is needed to confirm whether aerobic methanotrophs can oxidize methane under anaerobic conditions.
The mitogen-activated protein kinase kinases (MKK) represent a small gene family that is located at the center of the MAPK cascade and play an important role in responses to biotic and abiotic stresses and in plant growth and development. Here, we report the cloning of an MKK gene from Brassica napus, BnMKK1 (GenBank Accession No. HQ916282), by RT-PCR. The BnMKK1 cDNA is 1,447 bp in length with an open reading frame of 1,092 bp. The gene encodes a putative MKK protein that contains a conserved motif S/TxxxxxS/T (where x represents any amino acid) and a MPK docking domain in its N-terminal extension. The orthologues of the BnMKK1 protein are highly conserved among mosses, ferns, dicotyledons and monocotyledons. Southern hybridization revealed the presence of more than two copies of the BnMKK1 homologues in the genome of B. napus. Quantitative reverse transcription–polymerase chain reaction analyses showed that the BnMKK1 transcripts accumulated in response to cold, ABA (abscisic acid) and MeJA (methyl jasmonate) but declined in response to mannitol, NaCl, H₂O₂, and SA (salicylic acid). The inhibitors of MAPK activation, PD98059 and U0126, did not inhibit BnMKK1 transcription. BnMKK1 transgenic tobacco plants grew slower and showed significantly delayed flower times compared to the wild type. Their root development was insensitive to treatment with 100 mM IAA (indole-3-acetic acid). The detached leaves from the transgenic BnMKK1 tobacco plants strengthened the inhibition to bacterial development at later growth stages. The overexpression of BnMKK1 leads to rapid water loss and enhanced sensitivities to drought stress in transgenic tobacco plants. These results show that BnMKK1 plays an important role in the response of plants to pathogenic bacteria and drought stress.
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
Ecological risks of heavy metal toxicity such as Cd, Cu, Pb, Zn, Cr, As, and Hg through crops (wheat and rice) grown in the Tianjin sewage irrigation area in northern China were studied in this paper. Wheat and rice samples as well as related soil samples from 77 select blocks were collected. The second grade of standards for Tianjin soil environmental quality was used for soil risk assessment. Chinese National Food Safe Criterion was used for health risk assessment of wheat and rice grains. Daily intake rate and Target hazard quotient were used for the potential health risk assessment of local population through the intake of wheat and rice grown in the sewage-irrigated site. The results showed that continuous application of wastewater has led to accumulation of heavy metals in the soil, and Cd, Zn, and Hg were the main pollutants. Zn and Cd were more mobile than other metals. Pb in wheat and rice had an ecological risk to human health. As and Hg in some rice samples as well as Cd, Zn, and As in some wheat samples had potential risk. Target hazard quotient (THQ) of individual metal was below 1.0, meaning the relative absence of health risks associated with intake of a single heavy metal through intake of either wheat or rice. THQs of As for wheat and rice would sum up to above 1.0, indicating As may pose a risk to the local population by intake of wheat and rice.
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