Wyniki wyszukiwania

1

Reconstitution of brain mitochondria inner membrane into planar lipid bilayer

100%

Kulawiak B., Bednarczyk P.

Acta Neurobiologiae Experimentalis

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2005

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tom 65

|

nr 3

Ion channels are present in the inner mitochondrial membrane. They play an important role in cellular processes. Potassium and chloride channels are involved in regulation of mitochondrial volume, membrane potential and acidification. The mitochondrial potassium channels have been suggested as triggers and end effectors in cytoprotection. In our study we measured single channel activities after reconstitution of submitochondrial particles from rat brain mitochondria into planar lipid membranes. After incorporation, two different potassium selective currents were recorded with single channel conductance from 260 to 320 pS and from 70 to 90 pS in gradient (cis/trans) 50/450 and 50/150 mM KCl solutions, respectively. We also observed activity of the chloride ion channel. The measured single channel conductance was from 80 to 90 pS in gradient (cis/trans) 50/450 mM KCl solution. Our results suggest that various ion channels are present in the inner mitochondrial membrane of brain mitochondria.

2

Regulation of ROS production by a mitochondrial Ca2+-regulated largeconductance potassium channel

81%

Kulawiak B., Kunz W., Szewczyk A.-M.

Acta Biochimica Polonica

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2007

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tom 54

|

nr Supplement 4

3

Large -conductance potassium channel activators in neuroprotection

81%

Kulawiak B., Glab M., Szewczyk A.

Acta Biochimica Polonica

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2006

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tom 53

|

nr Supplement 1

4

Mitochondrial regulation of mitochondrial potassium channels

81%

Szewczyk A., Kulawiak B., Bednarczyk P., Koprowski P.

Acta Biochimica Polonica

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2018

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tom 65

|

nr S2

5

BK-DEC splice variant forms a functional BKCa channel in the inner mitochondrial membrane

71%

Kucman S. K., Jedraszko J., Bednarczyk P., Szewczyk A., Kulawiak B.

Acta Biochimica Polonica

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2018

|

tom 65

|

nr S2

6

Single-channel properties of the ROMK2-pore-forming subunit of the mitochondrial ATP-regulated potassium channel

71%

Kulawiak B., Laskowski M., Augustynek B., Bednarczyk P., Szewczyk A.

Acta Biochimica Polonica

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2018

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tom 65

|

nr S2

7

Identification of potassium channels in the mitochondria of human bronchial epithelial cells

71%

Sek A., Kampa R., Kulawiak B., Szewczyk A., Bednarczyk P.

Acta Biochimica Polonica

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2018

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tom 65

|

nr S2

8

Cardioprotective flavoniods as natural modulators of mitochondrial potassium channels

61%

Kampa R., Sek A., Kicinska A., Kulawiak B., Jarmuszkiewicz W., Szewczyk A., Bednarczyk P.

Acta Biochimica Polonica

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2018

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tom 65

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nr S2

9

Large-conductance K+ channel openers induce death of human glioma cells

51%

Debska-Vielhaber G., Godlewski M. M., Kicinska A., Skalska J., Kulawiak B., Piwonska M., Zablocki K., Kunz W. S., Szewczyk A., Motyl T.

Journal of Physiology and Pharmacology

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2009

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tom 60

|

nr 4

27-36

Large-conductance Ca2+-activated K+ channels (BKCa channels) are highly expressed in human glioma cells. It has been reported that BKCa channels are present in the inner mitochondrial membrane of the human glioma cell line LN229. In the present study we investigated whether BKCa-channel openers, such as CGS7181 (ethyl 2-hydroxy-1-[[(4-methylphenyl)amino]oxo]-6-trifluoromethyl-1H-indole-3-carboxylate) and CGS7184 (ethyl 1-[[(4-chlorophenyl) amino]oxo]-2-hydroxy-6-trifluoromethyl-1H-indole-3-carboxylate), affect the functioning of LN229 glioma cell mitochondria in situ. In the micromolar concentration range CGS7181 and CGS7184 induced glioma cell death. Morphological and cytometric analyses confirmed that both substances trigger the glioma cell death. This effect was not inhibited by the pan-caspase inhibitor z-VAD-fmk. Lack of DNA laddering, PARP cleavage, and caspase 3 activation suggested that glioma cell death was not of the apoptotic type. We examined the effect of CGS7184 on mitochondrial membrane potential and mitochondrial respiration. Potassium channel opener CGS7184 increased cell respiration and induced mitochondrial membrane depolarization. The latter was dependent on the presence of Ca2+ in the external medium. It was shown that CGS7184 induced an increase of cytosolic Ca2+ concentration due to endoplasmic reticulum store depletion. In conclusion, our results show that CGS7181 and CGS7184 induce glioma cell death by increasing the cytosolic calcium concentration followed by activation of calpains.

10

Mitochondrialne kanaly jonowe

51%

Skalska J., Debska-Vielhaber G., Glab M., Kulawiak B., Malinska D., Koszela-Piotrowska I., Bednarczyk P., Dolowy K., Szewczyk A.

Postępy Biochemii

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2006

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tom 52

|

nr 2

137-144

Ograniczanie wyników

Reconstitution of brain mitochondria inner membrane into planar lipid bilayer

Regulation of ROS production by a mitochondrial Ca2+-regulated largeconductance potassium channel

Large -conductance potassium channel activators in neuroprotection

Mitochondrial regulation of mitochondrial potassium channels

BK-DEC splice variant forms a functional BKCa channel in the inner mitochondrial membrane

Single-channel properties of the ROMK2-pore-forming subunit of the mitochondrial ATP-regulated potassium channel

Identification of potassium channels in the mitochondria of human bronchial epithelial cells

Cardioprotective flavoniods as natural modulators of mitochondrial potassium channels

Large-conductance K+ channel openers induce death of human glioma cells

Mitochondrialne kanaly jonowe