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1
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Evaluation of the udder health status in subclinical mastitis affected dairy cows through bacteriological culture, somatic cell count and thermographic imaging

100%
Bortolami A., Flore E., Gianesella M., Corro M., Catania S., Morgante M.
Polish Journal of Veterinary Sciences
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2015
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tom 18
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nr 4
Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson’s correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.
2
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Evaluation of venous blood gas levels, blood chemistry and haemocytometric parameters in milk fed veal calves at different periods of livestock cycle

100%
Giambelluca S., Flore E., Sadocco A., Gianesella M., Vazzana I., Orefice T., Morgante M.
Polish Journal of Veterinary Sciences
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2016
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tom 19
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nr 4
An evaluation of blood chemistry profile in relation to specific stages of livestock cycle can help better understand variations in physiological conditions in order to adjust management systems to animal needs. In addition to basal hematological investigation, the acid-base balance and blood gases are essential tools in evaluating metabolism in calves. The relationship between blood gas parameters, diet and growth should be further investigated. The aim of this study was to evaluate changes in acid-base status, blood gases, serum chemistry and hematological parameters in veal calves at different periods of livestock cycle. One hundred twenty-eight healthy cross breeding calves were enrolled in a farm in North-East Italy. Blood samplings were carried out from the jugular vein on day 1 (t1), 60 (t2) and 150 (t3) after arrival. Blood gas analysis was performed and hematological parameters were evaluated. One-way ANOVA and Tukey-Kramer post-hoc test were performed to assess differences between blood parameter values at the different periods. The main differences in blood gas parameter levels during the livestock cycle concerned pH, Base Excess and HCO3 with higher values recorded in t3. Urea, creatinine, gamma-glutamyl transpeptidase and bilirubin mean values were significantly higher in t1 than in t2 and t3. Aspartate aminotransferase increased from t1 to t2 and t3. Alkaline Phosphatase was higher in t2. Fe levels severely dropped in t2 and in t3, and the decrease led to a restrained but significant reduction in haemoglobin values. A correspondent decrease in the other haemocytometric parameters was found.
3
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Infrared thermography as a rapid and non-invasive diagnostic tool to detect inflammatory foot diseases in dairy cows

100%
Gianesella M., Arfuso F., Fiore E., Giambelluca S., Giudice E., Armato L., Piccione G.
Polish Journal of Veterinary Sciences
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2018
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tom 21
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nr 2
4

Glucose infusion response to some biochemical parameters in dairy cows during the transition period

84%
Fiore E., Barberio A., Morgante M., Rizzo M., Giudice E., Piccione G., Lora M., Gianesella M.
Animal Science Papers and Reports
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2015
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tom 33
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nr 2
The aim of this study was to evaluate the effect of pre-partum versus post-partum periods on the biochemical profile in dairy cows. The effect of glucose tolerance test (GTT) on glucose (Gluon-farm) and β-Hydroxybutyrate (BHBon-farm) levels was also assessed. 42 Holstein cows from two different farms (farm A and farm B) were enrolled in the study. Body Condition Score (BCS) was determined for both groups. The GTT was carried out 7±5 days pre-partum and 7±5 days post-partum. In both periods blood samples were taken at T0 (pre-glucose administration), at T10 and T80 (10 and 80 minutes post-glucose administration). All samples were analysed for total protein, albumin, globulin,glucose, urea, aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca),phosphorus (P), sodium (Na), chloride (Cl), potassium (K), magnesium (Mg) and BHBon-farm. Twoway ANOVA was applied to determine significant effects of the transition period (pre-partum vs.post-partum) and farm (A vs. B) on the studied parameters as well as the effect of time before and after the GTT on Gluon-farm. The average values of BCS, glucose, urea, Ca, Cl and K are significantly lower in the post-partum when compared to the pre-partum period (P<0.05). The increase in AST concentrations during the post-partum period (P<0.05) is considered a suitable indicator of hepatic steatosis. The glucose tended to increase significantly at T10 (P<0.05) and returned to pre-infusion concentrations at T80. These results confirm that the transition period is an important metabolic challenge to high-yielding dairy cows. Therefore, this study provides useful information in order to prevent the outset of nutritional imbalance that typically occurs in high production dairy cows.
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