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In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

100%

Tabbaa S., Corso T. D., Jenkins L., Tran R. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

2005

tom 64

nr 4

273-281

This study shows that an ICP4– replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the β-galactosidase gene can be used as a very effective vector for delivering the β-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed β-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

68%

Tabbaa S., Goulah C., Tran R. K., Lis A., Korody R., Stachowski B., Horowitz J. M., Torres G., Stachowiak E. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

2000

tom 59

nr 4

Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors have been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS.

Ograniczanie wyników

2 Folia Morphologica

2 Bloom D. C.

2 Stachowiak M. K.

2 Tabbaa S.

2 Tran R. K.

1 Corso T. D.

1 Goulah C.

1 Horowitz J. M.

1 Jenkins L.

1 Korody R.

1 Lis A.

1 Stachowiak E. K.

1 Stachowski B.

1 Torres G.

1 2005

1 2000

Wyniki wyszukiwania

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors